BARD1 (NM_000465) Human Untagged Clone

SKU SC119847

BARD1 (untagged)-Human BRCA1 associated RING domain 1 (BARD1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: BARD1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000465 edited; GAATTCGGCACGAGGCTTGGCCGGTTTCGAGTCGCTGACCTGCAGCTTCCCTGTGGTTTC; CCGAGGCCTCCTTGCTTCCCGCTCTCCGAGGAGCCTTTCATCCGAAGGCGGGACGATGCC; GGATAATCGGCAGCCGAGGAACCGGCAGCCGAGGATCCGCTCCGGGAACGAGCCTCGTTC; CGCGCCCGCCATGGAACCGGATGGTCGCGGTGCCTGGGCCCACAGTCGCGCCGCGCTCGA; CCGCCTGGAGAAGCTGCTGCGCTGCTCGCGTTGTACTAACATTCTGAGAGAGCCTGTGTG; TTTAGGAGGATGTGAGCACATCTTCTGTAGTAATTGTGTAAGTGACTGCATTGGAACTGG; ATGTCCAGTGTGTTACACCCCGGCCTGGATACAAGACTTGAAGATAAATAGACAACTGGA; CAGCATGATTCAACTTTGTAGTAAGCTTCGAAATTTGCTACATGACAATGAGCTGTCAGA; TTTGAAAGAAGATAAACCTAGGAAAAGTTTGTTTAATGATGCAGGAAACAAGAAGAATTC; AATTAAAATGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCTTC; AGTGCAAACCCAGCCTGCAATAAAAAAAGATGCAAGTGCTCAGCAAGACTCATATGAATT; TGTTTCCCCAAGTCCTCCTGCAGATGTTTCTGAGAGGGCTAAAAAGGCTTCTGCAAGATC; TGGAAAAAAGCAAAAAAAGAAAACTTTAGCTGAAATCAACCAAAAATGGAATTTAGAGGC; AGAAAAAGAAGATGGTGAATTTGACTCCAAAGAGGAATCTAAGCAAAAGCTGGTATCCTT; CTGTAGCCAACCATCTGTTATCTCCAGTCCTCAGATAAATGGTGAAATAGACTTACTAGC; AAGTGGCTCCTTGACAGAATCTGAATGTTTTGGAAGTTTAACTGAAGTCTCTTTACCATT; GGCTGAGCAAATAGAGTCTCCAGACACTAAGAGCAGGAATGAAGTAGTGACTCCTGAGAA; GGTCTGCAAAAATTATCTTACATCTAAGAAATCTTTGCCATTAGAAAATAATGGAAAACG; TGGCCATCACAATAGACTTTCCAGTCCCATTTCTAAGAGATGTAGAACCAGCATTCTGAG; CACCAGTGGAGATTTTGTTAAGCAAACGGTGCCCTCAGAAAATATACCATTGCCTGAATG; TTCTTCACCACCTTCATGCAAACGTAAAGTTGGTGGTACATCAGGGAGCAAAAACAGTAA; CATGTCCGATGAATTCATTAGTCTTTCACCAGGTACACCACCTTCTACATTAAGTAGTTC; AAGTTACAGGCGAGTGATGTCTAGTCCCTCAGCAATGAAGCTGTTGCCCAATATGGCTGT; GAAAAGAAATCATAGAGGAGAGACTTTGCTCCATATTGCTTCTATTAAGGGCGACATACC; TTCTGTTGAATACCTTTTACAAAATGGAAGTGATCCAAATGTTAAAGACCATGCTGGATG; GACACCATTGCATGAAGCTTGCAATCATGGGCACCTGAAGGTAGTGGAATTATTGCTCCA; GCATAAGGCATTGGTGAACACCACCGGGTATCAAAATGACTCACCACTTCACGATGCAGC; CAAGAATGGGCACGTGGATATAGTCAAGCTGTTACTTTCCTATGGAGCCTCCAGAAATGC; TGTTAATATATTTGGTCTGCGGCCTGTCGATTATACAGATGATGAAAGTATGAAATCGCT; ATTGCTGCTACCAGAGAAGAATGAATCATCCTCAGCTAGCCACTGCTCAGTAATGAACAC; TGGGCAGCGTAGGGATGGACCTCTTGTACTTATAGGCAGTGGGCTGTCTTCAGAACAACA; GAAAATGCTCAGTGAGCTTGCAGTAATTCTTAAGGCTAAAAAATATACTGAGTTTGACAG; TACAGTAACTCATGTTGTTGTTCCTGGTGATGCAGTTCAAAGTACCTTGAAGTGTATGCT; TGGGATTCTCAATGGATGCTGGATTCTAAAATTTGAATGGGTAAAAGCATGTCTACGAAG; AAAAGTATGTGAACAGGAAGAAAAGTATGAAATTCCTGAAGGTCCACGCAGAAGCAGGCT; CAACAGAGAACAGCTGTTGCCAAAGCTGTTTGATGGATGCTACTTCTATTTGTGGGGAAC; CTTCAAACACCATCCAAAGGACAACCTTATTAAGCTCGTCACTGCAGGTGGGGGCCAGAT; CCTCAGTAGAAAGCCCAAGCCAGACAGTGACGTGACTCAGACCATCAATACAGTCGCATA; CCATGCGAGACCCGATTCTGATCAGCGCTTCTGCACACAGTATATCATCTATGAAGATTT; GTGTAATTATCACCCAGAGAGGGTTCGGCAGGGCAAAGTCTGGAAGGCTCCTTCGAGCTG; GTTTATAGACTGTGTGATGTCCTTTGAGTTGCTTCCTCTTGACAGCTGAATATTATACCA; GATGAACATTTCAAATTGAATTTGCACGGTTTGTGAGAGCCCAGTCATTGTACTGTTTTT; AATGTTCACATTTTTACAAATAGGTAGAGTCATTCATATTTGTCTTTGAATCAAAAAAAA; AAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000465 unedited; GGAATTTGTATACGACTCCTATAGGCGGCCGCGAATTCGGCACGAGGCTTGGCCGGTTTC; GAGTCGCTGACCTGCAGCTTCCCTGTGGTTTCCCGAGGCCTCCTTGCTTCCCGCTCTCCG; AGGAGCCTTTCATCCGAAGGCGGGACGATGCCGGATAATCGGCAGCCCGAGAACCGGCAG; CCGAGGATCCGCTCCGGGAACGAGCCTCGTTCCGCGCCCGCCATGGAACCGGATGGTCGC; GGTGCCTGGGCCCACAGTCGCGCCGCGCTCGACCGCCTGGAGAAGCTGCTGCGCTGCTCG; CGTTGTACTAACATTCTGAGAGAGCCTGTGTGTTTAGGAGGATGTGAGCACATCTTCTGT; AGTAATTGTGTAAGTGACTGCATTGGAACTGGATGTCCAGTGTGTTACACCCCGGCCTGG; ATACAAGACTTGAAGATAAATAGACAACTGGACAGCATGATTCAACTTTGTAGTAAGCTT; CGAAATTTGCTACATGACAATGAGCTGTCAGATTTGAAAGAAGATAAACCTAGGAAAAGT; TTGTTTAATGATGCAGGAAACAAGAAGAATTCAATTAAAATGTGGTTTAGCCCTCGAAGT; AAGAAAGTCAGATATGTTGTGAGTAAAGCTTCAGTGCAAACCCAGCCTGCAATAAAAAAA; GATGCAAGTGCTCAGCAAGACTCATATGAATTTGTTTCCCCAAGTCCTCCTGCAGATGTT; TCTGAGAGGGCTAAAAAGGCTTCTGNCAGATCTGGNAAAAAGCANAAAAAGAAACTTTAG; CTGAAATCAACAAAAATGGAATTTAGAGGCAGAAAAAGAGATGGTGAATTTGACTCCCAA; GAGAAATCTAGCAAAGCTGGTATCCTTCTGTAGCCACCATCTGTTATCTCCAGTCCTCAG; ATAAATGGTGAATAGACTACTAGCAGTGGCTCCTGACA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000465 unedited; CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGATTCAAANACAAATATGAAT; GACTCTACCTATTTGTAAAAATGTGAACATTAAAAACAGTACAATGACTGGGCTCTCACA; AACCGTGCAAATTCAATTTGAAATGTTCATCTGGTATAATATTCAGCTGTCAAGAGGAAG; CAACTCAAAGGACATCACACAGTCTATAAACCAGCTCGAAGGAGCCTTCCAGACTTTGCC; CTGCCGAACCCTCTCTGGGTGATAATTACACAAATCTTCATAGATGATATACTGTGTGCA; GAAGCGCTGATCAGAATCGGGTCTCGCATGGTATGCGACTGTATTGATGGTCTGAGTCAC; GTCACTGTCTGGCTTGGGCTTTCTACTGAGGATCTGGCCCCCACCTGCAGTGACGAGCTT; AATAAGGTTGTCCTTTGGATGGTGTTTGAAGGTTCCCCACAAATAGAAGTAGCATCCATC; AAACAGCTTTGGCAACAGCTGTTCTCTGTTGAGCCTGCTTCTGCGTGGACCTTCAGGAAT; TTCATACTTTTCTTCCTGTTCACATACTTTTCTTCGTAGACATGCTTTTACCCATTCAAA; TTTTAGAATCCAGCATCCATTGAGAATCCCAAGCATACACTTCAAGGTACTTTGAACTGC; ATCACCAGGAACAACAACATGAGTTACTGTACTGTCAAACTCAGTATATTTTTTAGCCTT; AAGAATTACTGNCAGCTCACTGAGCATTTTCTGTTGTTCTGAAGACAGCCCACTGCCTAT; AAGTACAAGAGGTCCATCCCTACGCTGCCCAGTGTTCATTACTGAACAGTGGCTAGCTGA; AGATGATTCATTCTTCTCCTGGTAGCAGCAATAGCCGATTCATACCTTTATCATCTGTAT; AAACGACAGCCGCAAACCAATATATAACAGCATTCCTGGAGCTCCATAGAAAGAAAGCTT; GCTTTTTCAGTGCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_000465
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000465.1, NP_000456.1
• RefSeq Size: 2530 bp
• RefSeq ORF: 2334 bp
• Locus ID: 580
• UniProt ID: Q99728
• Cytogenetics: 2q35
• Domains: ANK, BRCT
• Protein Families: Druggable Genome
• Summary: This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]; Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.