5 HT 2A (HTR2A) (NM_000621) Human Untagged Clone

CAT#: SC119793

HTR2A (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 2A (HTR2A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: 5 HT 2A
• Synonyms: 5-HT2A; HTR2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119793 sequence for NM_000621 edited (data generated by NextGen Sequencing)
  
  
  ATGGATATTCTTTGTGAAGAAAATACTTCTTTGAGCTCAACTACGAACTCCCTAATGCAATTAAATGATG
  ACAACAGGCTCTACAGTAATGACTTTAACTCCGGAGAAGCTAACACTTCTGATGCATTTAACTGGACAGT
  CGACTCTGAAAATCGAACCAACCTTTCCTGTGAAGGGTGCCTCTCACCGTCGTGTCTCTCCTTACTTCAT
  CTCCAGGAAAAAAACTGGTCTGCTTTACTGACAGCCGTAGTGATTATTCTAACTATTGCTGGAAACATAC
  TCGTCATCATGGCAGTGTCCCTAGAGAAAAAGCTGCAGAATGCCACCAACTATTTCCTGATGTCACTTGC
  CATAGCTGATATGCTGCTGGGTTTCCTTGTCATGCCCGTGTCCATGTTAACCATCCTGTATGGGTACCGG
  TGGCCTCTGCCGAGCAAGCTTTGTGCAGTCTGGATTTACCTGGACGTGCTCTTCTCCACGGCCTCCATCA
  TGCACCTCTGCGCCATCTCGCTGGACCGCTACGTCGCCATCCAGAATCCCATCCACCACAGCCGCTTCAA
  CTCCAGAACTAAGGCATTTCTGAAAATCATTGCTGTTTGGACCATATCAGTAGGTATATCCATGCCAATA
  CCAGTCTTTGGGCTACAGGACGATTCGAAGGTCTTTAAGGAGGGGAGTTGCTTACTCGCCGATGATAACT
  TTGTCCTGATCGGCTCTTTTGTGTCATTTTTCATTCCCTTAACCATCATGGTGATCACCTACTTTCTAAC
  TATCAAGTCACTCCAGAAAGAAGCTACTTTGTGTGTAAGTGATCTTGGCACACGGGCCAAATTAGCTTCT
  TTCAGCTTCCTCCCTCAGAGTTCTTTGTCTTCAGAAAAGCTCTTCCAGCGGTCGATCCATAGGGAGCCAG
  GGTCCTACACAGGCAGGAGGACTATGCAGTCCATCAGCAATGAGCAAAAGGCATGCAAGGTGCTGGGCAT
  CGTCTTCTTCCTGTTTGTGGTGATGTGGTGCCCTTTCTTCATCACAAACATCATGGCCGTCATCTGCAAA
  GAGTCCTGCAATGAGGATGTCATTGGGGCCCTGCTCAATGTGTTTGTTTGGATCGGTTATCTCTCTTCAG
  CAGTCAACCCACTAGTCTACACACTGTTCAACAAGACCTATAGGTCAGCCTTTTCACGGTATATTCAGTG
  TCAGTACAAGGAAAACAAAAAACCATTGCAGTTAATTTTAGTGAACACAATACCGGCTTTGGCCTACAAG
  TCTAGCCAACTTCAAATGGGACAAAAAAAGAATTCAAAGCAAGATGCCAAGACAACAGATAATGACTGCT
  CAATGGTTGCTCTAGGAAAGCAGCATTCTGAAGAGGCTTCTAAAGACAATAGCGACGGAGTGAATGAAAA
  GGTGAGCTGTGTGTGA
  
  Clone variation with respect to NM_000621.4
  74:a=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000621 unedited
  GCANAANAGGGGACTCTACACCAGTTTAATTACTGTGAGAGATGCAGCGAGTCACAGAAT
  AACAAATGTATCTCATGTGTGGACCCTGAAGACAAATGACATTTATCTTCCCGAGCGCTC
  AAAAAAAACCCTGCAACCTCTATGCTAAAAGTTCATTCTGCTTTTTTGTCCTCGGTTTTT
  TTTATTAAAATAATAAAACCAAACAGTGGACTCTCCTAAAATTGTGAATGAAGAAAACTT
  ACAGCCACCACAGTTCAGTTCTTTAACTATCATTGTAATAATGGAAGACAAAAATCCAGC
  CCCGGGAGAACAGCATGTACACCAGCCTCAGTGTTACAGAGTGTGGGTACATCAAGGTGA
  ATGGTGAGCAGAAACTATAACCTGTTAGTCCTTCTACACCTCATCTGCTACAAGTTCTGG
  CTTAGACATGGATATTCTTTGTGAAGAAAATACTTCTTTGAGCTCAACTACGAACTCCCT
  AATGCAATTAAATGATGACAACAGGCTCTACAGTAATGACTTTAACTCCGGAGAAGCTAA
  CACTTCTGATGCATTTAACTGGACAGTCGACTCTGAAAATCGAACCAACCTTTCCTGTGA
  AGGGTGCCTCTCACCGTCGTGTCTCTCCTTACTTCATCTCCAGGAAAAAAACTGGTCTGC
  TTTACTGACAGCCGTAGTGATTATTCTAACTATTGCTGNGAACATACTCGTCATCATGGC
  AGTGTCCCTAGAGAAAAAGCTGCAGAATGCCACCAACTATTTCCTGATGTCACTTGCCAT
  AGCTGATATGCTGCTGGGTTTCCTTGTCATGCCCGTGTCCATGTTAACCATCCTGTATGG
  GTACCCGTGGCCTCTGCCGAGCAGCTTTGTGCAGTCTGGATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000621 unedited
  GCGGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGTGATAGGGTCTCACTCTG
  TTGCCCAGGAGGAAATGGCAGTGGTGTGAACTTGGCTCACTGCAGCCTTGAGCTTCTGGG
  CTCAAGTGATCCTTGCGTCTCAGCCTCCCCTGTAGCTGGGACCACAGTTGTGTGCCACAA
  GGATCAGATAATTTTTTGATTTTTTTTGTAGAGACAGGGAGGTCTCACTTTGTTACCCAG
  GTTGGTCTCAAACTCTTGGCCTCAAGTGATCCTCCCATCTCAGCCTCCCAAAGTGCTAGG
  ATTACATGCACGAGCCTCCGTGCCTGGCCGAGCCATTTCGTTTGAAGGCAAGGATTTTGT
  TTTAATTATTTTTATATTTCTCATATCTAGAAATTTGCTTCACACATAAATGTACACTCA
  ATAGATGATTGTGGAACCAAGCAGAATGGTTTTAATAAATAGTATAAACCGATGGATCTG
  AAAGCATCAGTTTACCTGATGAGTGTCATAGGAAAGTCACCTGCTTTTTACAGAGATGTC
  CTAAGACTCTACCACTGTCAGTAGCCGGAAACCTGAGTTCATCCTCAATCCTGACACAGT
  TACATCAATATACATTTACTGCTCAAAAGAGTGCATGCATGATGCAGTCATTTCACAGCA
  AGTTACCAAATACCTCGATAGTGCTGTTTTAGAAGAGCAATTGCTAGAAAAGCATTTGGT
  AATGGTAAATCATCCTTTTGCTAGTTACCTAGAGGAACAATATTAAACNAGACACACTGG
  AATTCAGAGTAAGCCCAGAAGTGAAGAGAAGCAGCATAGCTATGGATACATTTGAGTCTT
  TTCACTCTGAGATTACTTGAATGCCCAATGCCACACCCTGGATATTTCAAATGTTCTATG
  AAAACAGATGAAAATGGTGCAGTTTTATTAATATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000621
• Insert Size: 3300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_000621.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000621.2, NP_000612.1
• RefSeq Size: 5442 bp
• RefSeq ORF: 1416 bp
• Locus ID: 3356
• UniProt ID: P28223
• Cytogenetics: 13q14.2
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Gap junction, Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes one of the receptors for serotonin, a neurotransmitter with many roles. Mutations in this gene are associated with susceptibility to schizophrenia and obsessive-compulsive disorder, and are also associated with response to the antidepressant citalopram in patients with major depressive disorder (MDD). MDD patients who also have a mutation in intron 2 of this gene show a significantly reduced response to citalopram as this antidepressant downregulates expression of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.