ATP1A2 (NM_000702) Human Untagged Clone

CAT#: SC119715

ATP1A2 (untagged)-Human ATPase, Na+/K+ transporting, alpha 2 polypeptide (ATP1A2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP1A2
• Synonyms: FHM2; MHP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119715 sequence for NM_000702 edited (data generated by NextGen Sequencing)
  ATGGGCCGTGGGGCTGGCCGTGAGTACTCACCTGCCGCCACCACGGCAGAGAATGGGGGC
  GGCAAGAAGAAACAGAAGGAGAAGGAACTGGATGAGCTGAAGAAGGAGGTGGCAATGGAT
  GACCACAAGCTGTCCTTGGATGAGCTGGGCCGCAAATACCAAGTGGACCTGTCCAAGGGC
  CTCACCAACCAGCGGGCTCAGGACGTTCTGGCTCGAGATGGGCCCAACGCCCTCACACCA
  CCTCCCACAACCCCTGAGTGGGTCAAGTTCTGCCGTCAGCTTTTCGGGGGGTTCTCCATC
  CTGCTGTGGATTGGGGCTATCCTCTGCTTCCTGGCCTACGGCATCCAGGCTGCCATGGAG
  GATGAACCATCCAACGACAATCTATATCTGGGTGTGGTGCTGGCAGCTGTGGTCATTGTC
  ACTGGCTGCTTCTCCTACTACCAGGAGGCCAAGAGCTCCAAGATCATGGATTCCTTCAAG
  AACATGGTACCTCAGCAAGCCCTTGTGATCCGGGAGGGAGAGAAGATGCAGATCAACGCA
  GAGGAAGTGGTGGTGGGAGACCTGGTGGAGGTGAAGGGTGGAGACCGCGTCCCTGCTGAC
  CTCCGGATCATCTCTTCTCATGGCTGTAAGGTGGATAACTCATCCTTAACAGGAGAGTCG
  GAGCCCCAGACCCGCTCCCCCGAGTTCACCCATGAGAACCCCCTGGAGACCCGCAATATC
  TGTTTCTTCTCCACCAACTGTGTTGAAGGCACTGCCAGGGGCATTGTGATTGCCACAGGA
  GACCGGACGGTGATGGGCCGCATAGCTACTCTCGCCTCAGGCCTGGAGGTTGGGCGGACA
  CCCATAGCAATGGAGATTGAACACTTCATCCAGCTGATCACAGGGGTCGCTGTATTCCTG
  GGGGTCTCCTTCTTCGTGCTCTCCCTCATCCTGGGCTACAGCTGGCTGGAGGCAGTCATC
  TTCCTCATCGGCATCATAGTGGCCAACGTGCCTGAGGGGCTTCTGGCCACTGTCACTGTG
  TGCCTGACCCTGACAGCCAAGCGCATGGCACGGAAGAACTGCCTGGTGAAGAACCTGGAG
  GCGGTGGAGACGCTGGGCTCCACGTCCACCATCTGCTCGGACAAGACGGGCACCCTCACC
  CAGAACCGCATGACCGTCGCCCACATGTGGTTCGACAACCAAATCCATGAGGCTGACACC
  ACCGAAGATCAGTCTGGGGCCACTTTTGACAAACGATCCCCTACGTGGACGGCCCTGTCT
  CGAATTGCTGGTCTCTGCAACCGCGCCGTCTTCAAGGCAGGACAGGAGAACATCTCCGTG
  TCTAAGCGGGACACAGCTGGTGATGCCTCTGAGTCAGCTCTGCTCAAGTGCATTGAGCTC
  TCCTGTGGCTCAGTGAGGAAAATGAGAGACAGAAACCCCAAGGTGGCAGAGATTCCTTTC
  AACTCTACCAACAAGTACCAGCTGTCTATCCACGAGCGAGAAGACAGCCCCCAGAGCCAC
  GTGCTGGTGATGAAGGGGGCCCCAGAGCGCATTCTGGACCGGTGCTCCACCATCCTGGTG
  CAGGGCAAGGAGATCCCGCTCGACAAGGAGATGCAAGATGCCTTTCAAAATGCCTACATG
  GAGCTGGGGGGACTTGGGGAGCGTGTGCTGGGATTCTGTCAACTGAATCTGCCATCTGGA
  AAGTTTCCTCGGGGCTTCAAATTCGACACGGATGAGCTGAACTTTCCCACGGAGAAGCTT
  TGCTTTGTGGGGCTCATGTCTATGATTGACCCTCCCCGGGCTGCTGTGCCAGATGCTGTG
  GGCAAGTGCCGAAGCGCAGGCATCAAGGTGATCATGGTAACCGGGGATCACCCTATCACA
  GCCAAGGCCATTGCCAAAGGCGTGGGCATCATATCAGAGGGTAACGAGACTGTGGAGGAC
  ATTGCAGCCCGGCTCAACATTCCCATGAGTCAAGTCAACCCCAGAGAAGCCAAGGCATGC
  GTGGTGCACGGCTCTGACCTGAAGGACATGACATCGGAGCAGCTCGATGAGATCCTCAAG
  AACCACACAGAGATCGTCTTTGCTCGAACGTCTCCCCAGCAGAAGCTCATCATTGTGGAG
  GGATGTCAGAGGCAGGGAGCCATTGTGGCCGTGACGGGTGACGGGGTGAACGACTCCCCT
  GCATTGAAGAAGGCTGACATTGGCATTGCCATGGGCATCTCTGGCTCTGACGTCTCTAAG
  CAGGCAGCCGACATGATCCTGCTGGATGACAACTTTGCCTCCATCGTCACGGGGGTGGAG
  GAGGGCCGCCTGATCTTTGACAACTTGAAGAAATCCATCGCCTACACCCTGACCAGCAAC
  ATCCCCGAGATCACCCCCTTCCTGCTGTTCATCATTGCCAACATCCCCCTACCTCTGGGC
  ACTGTGACCATCCTTTGCATTGACCTGGGCACAGATATGGTCCCTGCCATCTCCTTGGCC
  TATGAGGCAGCTGAGAGTGATATCATGAAGCGGCAGCCACGAAACTCCCAGACGGACAAG
  CTGGTGAATGAGAGGCTCATCAGCATGGCCTACGGACAGATCGGGATGATCCAGGCACTG
  GGTGGCTTCTTCACCTACTTTGTGATCCTGGCAGAGAACGGTTTCCTGCCATCACGGCTA
  CTGGGAATCCGCCTCGACTGGGATGACCGGACCATGAATGATCTGGAGGACAGCTATGGA
  CAGGAGTGGACCTATGAGCAGCGGAAGGTGGTGGAGTTCACGTGCCACACGGCATTCTTT
  GCCAGCATCGTGGTGGTGCAGTGGGCTGACCTCATCATCTGCAAGACCCGCCGCAACTCA
  GTCTTCCAGCAGGGCATGAAGAACAAGATCCTGATTTTTGGGCTCCTGGAGGAGACGGCG
  TTGGCTGCCTTTCTCTCTTACTGCCCAGGCATGGGTGTAGCCCTCCGCATGTACCCGCTC
  AAAGTCACCTGGTGGTTCTGCGCCTTCCCCTACAGCCTCCTCATCTTCATCTATGATGAG
  GTCCGAAAGCTCATCCTGCGGCGGTATCCTGGTGGCTGGGTGGAGAAGGAGACATACTAC
  TGA
  
  Clone variation with respect to NM_000702.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000702 unedited
  GGCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCCGAGGTCTCCGACTGTC
  CCAGACGGGCTGGTGTGGGCTTGGGATCCTCCTGGTGACCTCTCCCGCTAAGGTCCCTCA
  GCCACTCTGCCCCAAGATGGGCCGTGGGGCTGGCCGTGAGTACTCACCTGCCGCCACCAC
  GGCAGAGAATGGGGGCGGCAAGAAGAAACAGAAGGAGAAGGAACTGGATGAGCTGAAGAA
  GGAGGTGGCAATGGATGACCACAAGCTGTCCTTGGATGAGCTGGGCCGCAAATACCAAGT
  GGACCTGTCCAAGGGCCTCACCAACCAGCGGGCTCAGGACGTTCTGGCTCGAGATGGGCC
  CAACGCCCTCACACCACCTCCCACAACCCCTGAGTGGGTCAAGTTCTGCCGTCAGCTTTT
  CGGGGGGTTCTCCATCCTGCTGTGGATTGGGGCTATCCTCTGCTTCCTGGCCTACGGCAT
  CCAGGCTGCCATGGAGGATGAACCATCCAACGACAATCTATATCTGGGTGTGGTGCTGGC
  AGCTGTGGTCATTGTCACTGGCTGCTTCTCCTACTACCAGGAGGCCAAGAGCTCCAAGAT
  CATGGATTCCTTCAAGAACATGGTACCTCAGCAAGCCCTTGTGATCCGGGAGGGAGAGAA
  GATGCAGATCAACGCATAGGAAGTGGTGGTGGGAGAACTGGTGGAGGTGAAGGGTGGAGA
  CCGCGTCCCTGCTGACCCCCGGATCACCTCTTTTATGGCTGTAAGGTGGAAACTCATCCT
  TACCAGAGAGTTCGACCCCCAGACCCGTCCCCCGAGTTACCCATGAGAACCCCTGGAGAC
  CCGGCATATCTGGTTTCTTCTCCCCCACTGTGTTGAAAGCCCTGCCCAGGCCTTTGGGAT
  TGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000702 unedited
  CCGCGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTGAGAGGAGTCTCACCCTT
  TCGCCCAGGCTGGAGTGTAATGGCACGATCTCAGCTCACTGCAACCTCTTCCTCACAGGT
  TCAAGCAATTCTCCTGCCTCAGCCTCCTGAGTAGCTGAGATTACAGGCGTGTGCCACCAT
  GACTGGCTAATTTTTTGTACTTTTAGTAGAGACAGGGTTTCACCATGTTGGCCAGGCTCG
  CCTCGAACTCCTGACCTCATGATCCACCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAG
  GCATGAGCCACCGCACCCAGAAAAAGCAAATCTCTTAGTATTTTTCCTCTTGTCCAAAGG
  TTCTGACCATGTTCATGACCTAAGCTTGTCCCTGGAAGCATACATGTCCCTGGGAGACAG
  GAAGGTTCAGGGAACTTCTCCACCCTGATTCTAGCCAATGGCCAGCGAACTCCTTTTCCA
  GGGTTGGCTTGGCAGTGTCGGGGAATGATGCAGGTTCATCGGGATGGGCATGAAAAGAAA
  GGGAATGGAAGAGGCTGGGAAAGAACTTGTGGCCATCCCTTTCAGAACAGCATGGAGCTC
  TGANGACTTAGTATTACAGAAGGTGAAGTGTTATTAAGTACATGAGTGACATCCGGACCT
  TGTGGACTAAGAAGGACCCTACCAAAGGTCAGCGGGGAACTCTGTGCACACAAGGTGGCA
  TTTCTGGAGCCTCTGATTGCCTCTGGCCTGCTCTGCTGCACCCTGAGAGACTGAGTTTGT
  CTGCTGTGGGGGATCTAAAAGTGGACGATAGCTGGATCCCCACTCTATTGGAACTAGTCA
  AAGCCCGGCATGGCCCACCTACTTCTTTTGTGGTCCTCCTCCACCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000702
• Insert Size: 5000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000702.2, NP_000693.1
• RefSeq Size: 5468 bp
• RefSeq ORF: 3063 bp
• Locus ID: 477
• UniProt ID: P50993
• Cytogenetics: 1q23.2
• Domains: E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cardiac muscle contraction
• Gene Summary: The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 2 subunit. Mutations in this gene result in familial basilar or hemiplegic migraines, and in a rare syndrome known as alternating hemiplegia of childhood. [provided by RefSeq, Oct 2008]