DCK (NM_000788) Human Untagged Clone

CAT#: SC119688

DCK (untagged)-Human deoxycytidine kinase (DCK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119688 sequence for NM_000788 edited (data generated by NextGen Sequencing)
  ATGGCCACCCCGCCCAAGAGAAGCTGCCCGTCTTTCTCAGCCAGCTCTGAGGGGACCCGC
  ATCAAGAAAATCTCCATCGAAGGGAACATCGCTGCAGGGAAGTCAACATTTGTGAATATC
  CTTAAACAATTGTGTGAAGATTGGGAAGTGGTTCCTGAACCTGTTGCCAGATGGTGCAAT
  GTTCAAAGTACTCAAGATGAATTTGAGGAACTTACAATGTCTCAGAAAAATGGTGGGAAT
  GTTCTTCAGATGATGTATGAGAAACCTGAACGATGGTCTTTTACCTTCCAAACATATGCC
  TGTCTCAGTCGAATAAGAGCTCAGCTTGCCTCTCTGAATGGCAAGCTCAAAGATGCAGAG
  AAACCTGTATTATTTTTTGAACGATCTGTGTATAGTGACAGGTATATTTTTGCATCTAAT
  TTGTATGAATCTGAATGCATGAATGAGACAGAGTGGACAATTTATCAAGACTGGCATGAC
  TGGATGAATAACCAATTTGGCCAAAGCCTTGAATTGGATGGAATCATTTATCTTCAAGCC
  ACTCCAGAGACATGCTTACATAGAATATATTTACGGGGAAGAAATGAAGAGCAAGGCATT
  CCTCTTGAATATTTAGAGAAGCTTCATTATAAACATGAAAGCTGGCTCCTGCATAGGACA
  CTGAAAACCAACTTCGATTATCTTCAAGAGGTGCCTATCTTAACACTGGATGTTAATGAA
  GACTTTAAAGACAAATATGAAAGTCTGGTTGAAAAGGTCAAAGAGTTTTTGAGTACTTTG
  TGA
  
  Clone variation with respect to NM_000788.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000788 unedited
  GTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCGAGGCTCTGGNGC
  CGCCACAAAACTAAGGAATGGCCACCCCGCCCAAAAGAAGCTGCCCGTCTTTCTCAGCCA
  GCTCTGAGGGGACCCGCATCAAGAAAATCTCCATCGAAGGGAACATCGCTGCAGGGAAGT
  CAACATTTGTGAATATCCTTAAACAATTGTGTGAAGATTGGGAAGTGGTTCCTGAACCTG
  TTGCCAGATGGTGCAATGTTCAAAGTACTCAAGATGAATTTGAGGAACTTACAATGTCTC
  AGAAAAATGGTGGGAATGTTCTTCAGATGATGTATGAGAAACCTGAACGATGGTCTTTTA
  CCTTCCAAACATATGCCTGTCTCAGTCGAATAAGAGCTCAGCTTGCCTCTCTGAATGGCA
  AGCTCAAAGATGCAGAGAAACCTGTATTATTTTTTGAACGATCTGTGTATAGTGACAGGT
  ATATTTTTGCATCTAATTTGTATGAATCTGAATGCATGAATGAGACAGAGTGGACAATTT
  ATCAAGACTGGCATGACTGGATGAATAACCAATTTGGCCAAAGCCTTGAATTGGATGGAA
  TCATTTATCTTCAAGCCACTCCAGAGACATGCTTACATAGAATATATTTACGGNGAAGAA
  ATGAAGAGCAAGGCATTCCTCTTGAATATTTAGAGAAGCTTCATTATAAACATGAAAGCT
  GGCTCCTGCATAGGACACTGAAAACCAACTTCGATTATCTTCAAGAGGTGCCTATCTTAA
  CACTGGATGTTAATGAAGACTCTAAAGACCAATATGAAAGTCTGTNTGAAAGGTCANAGA
  GTTNTTGAGTACTTTGTGATCTTGCTGAAGACTACAGGCAGCCAATGGTTCCAGATACTC
  AGCTTGTGTATCTTCGTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000788 unedited
  ACGCCGCCGCTTTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGTCATGACAAATAAAT
  AATAAATTTTTATTAGCGTCTTTTCAATTCTACAAATTTTAAAAGTCATTATACATTTTT
  AAAGGTTACTAAAAATCACTGAAATATGTCTCCACTTAAAATGGAATCAAAGTAATTTAT
  ACAATTTTCACCTTTAATATTCATAATTTATTTAAAAGTAGAATAAATTTTAATTCTAAA
  ATGATTATTACATCTTTTTAGAACTGAAATTACATTTCAATGAGTGTAGCTCCACTGTAG
  TTCATAAGGTTTATACTTAAGAAATATTTAATTAACAAATTAATTTATACCAAACAAAAA
  CAAACTAAAAAAATGCATACGTATAATGAGACAGTTTATGTATGGTAATTACAGATTTGA
  AATTAAGTCTCCTTTGCAAAGATCAGTGCGTCTGGACTGGTTAATCCACTAAATTATCAT
  CTTTGAATAAGAAACCAAAAACAAAAATGACTTGGAAAGAAAAATAGTAGTTTAAAAGTG
  AAAATGACTCTTCCCCTTATTTTAAAATACATAAAATTTTCTAAAACGTAAAATGGAAAG
  GTTTAATACACAGGAAAAACACTGCACACCTTAACTAAACCAACTAGAAAGAAAATATAT
  TTATATACACGACTAAAATGAGTCCATTCCTTTATTTTACAAATATCCACATAAAATGCT
  TAAAGAAAAATAGTTAAGTGTGGCTCATTAAATTAATGGAATGCTTTCTACGCCTCTGGC
  TTCACTAAATTTATTTCCTATTAACCAAAAATAATGCTTTCAAGGATATTTTCCATATAA
  GAGCACTTCTCATTTTTATGCACCATTATGGCCGGAAAAAAAGAAAAACTTATAAACGCG
  AACCCTTCCCAGCGCAAGCCAACTTCCAACGCAAAAAGTATCTACCGGTGTTGCACAAAA
  AAAGGTTTGG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_000788
• Insert Size: 2400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000788.1, NP_000779.1
• RefSeq Size: 2460 bp
• RefSeq ORF: 783 bp
• Locus ID: 1633
• UniProt ID: P27707
• Cytogenetics: 4q13.3
• Domains: dNK
• Protein Families: Druggable Genome
• Protein Pathways: Purine metabolism, Pyrimidine metabolism
• Gene Summary: Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq, Jul 2008]