CYP11A1 (NM_000781) Human Untagged Clone

CAT#: SC119684

CYP11A1 (untagged)-Human cytochrome P450, family 11, subfamily A, polypeptide 1 (CYP11A1), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP11A1
• Synonyms: CYP11A; CYPXIA1; P450SCC
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000781 edited
  TTCGCACCAGGCAGGTACAGTCACAGCTGTGGGGACAGCATGCTGGCCAAGGGTCTTCCC
  CCACGCTCAGTCCTGGTCAAAGGCTGCCAGACCTTTCTGAGTGCCCCCAGGGAGGGGCTG
  GGGCGTCTCAGGGTGCCCACTGGCGAGGGAGCTGGCATCTCCACCCGCAGTCCTCGCCCC
  TTCAATGAGATCCCCTCTCCTGGTGACAATGGCTGGCTAAACCTGTACCATTTCTGGAGG
  GAGACGGGCACACACAAAGTCCACCTTCACCATGTCCAGAATTTCCAGAAGTATGGCCCG
  ATTTACAGGGAGAAGCTCGGCAACGTGGAGTCGGTTTATGTCATCGACCCTGAAGATGTG
  GCCCTTCTCTTTAAGTCCGAGGGCCCCAACCCAGAACGATTCCTCATCCCGCCCTGGGTC
  GCCTATCACCAGTATTACCAGAGACCCATAGGAGTCCTGTTGAAGAAGTCGGCAGCCTGG
  AAGAAAGACCGGGTGGCCCTGAACCAGGAGGTGATGGCTCCAGAGGCCACCAAGAACTTT
  TTGCCCCTGTTGGATGCAGTGTCTCGGGACTTCGTCAGTGTCCTGCACAGGCGCATCAAG
  AAGGCGGGCTCCGGAAATTACTCGGGGGACATCAGTGATGACCTGTTCCGCTTTGCCTTT
  GAGTCCATCACTAACGTCATTTTTGGGGAGCGCCAGGGGATGCTGGAGGAAGTAGTGAAC
  CCCGAGGCCCAGCGATTCATTGATGCCATCTACCAGATGTTCCACACCAGCGTCCCCATG
  CTCAACCTTCCCCCAGACCTGTTCCGTCTGTTCAGGACCAAGACCTGGAAGGACCATGTG
  GCTGCATGGGACGTGATTTTCAGTAAAGCTGACATATACACCCAGAACTTCTACTGGGAA
  TTGAGACAGAAAGGAAGTGTTCACCACGATTACCGTGGCATCCTCTACAGACTCCTGGGA
  GACAGCAAGATGTCCTTCGAGGACATCAAGGCCAACGTCACAGAGATGCTGGCAGGAGGG
  GTGGACACGACGTCCATGACCCTGCAGTGGCACTTGTATGAGATGGCACGCAACCTGAAG
  GTGCAGGATATGCTGCGGGCAGAGGTCTTGGCTGCGCGGCACCAGGCCCAGGGAGACATG
  GCCACGATGCTACAGCTGGTCCCCCTCCTCAAAGCCAGCATCAAGGAGACACTAAGACTT
  CACCCCATCTCCGTGACCCTGCAGAGATATCTTGTAAATGACTTGGTTCTTCGAGATTAC
  ATGATTCCTGCCAAGACACTGGTGCAAGTGGCCATCTATGCTCTGGGCCGAGAGCCCACC
  TTCTTCTTCGACCCGGAAAATTTTGACCCAACCCGATGGCTGAGCAAAGACAAGAACATC
  ACCTACTTCCGGAACTTGGGCTTTGGCTGGGGTGTGCGGCAGTGTCTGGGACGGCGGATC
  GCTGAGCTAGAGATGACCATCTTCCTCATCAATATGCTGGAGAACTTCAGAGTTGAAATC
  CAACACCTCAGCGATGTGGGCACCACATTCAACCTCATTCTGATGCCTGAAAAGCCCATC
  TCCTTCACCTTCTGGCCCTTTAACCAGGAAGCAACCCAGCAGTGATCAGAGAGGATGGCC
  TGCAGCCACATGGGAGGAAGGCCCAGGGGTGGGGCCCATGGGGTCTCTGCATCTTCAGTC
  GTCTGTCCCAAGTCCTGCTCCTTTCTGCCCAGCCTGCTCAGCAGGTTGAATGGGTTCTCA
  GTGGTCACCTTCCTCAGCTCAGCTGGGCCACTCCTCTTCACCCACCCCAGGAGACAATAA
  ACAGCTGAACCATGAAAAAAAAAAAAAAAAAAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000781 unedited
  TATTTTGTAATACGACTCACTATAGAGGCGGCCGCGAATTCGCACCAGGCAGGTACAGTC
  ACAGCTGTGGGGACAGCATGCTGGCCAAGGGTCTTCCCCCACGCTCAGTCCTGGTCAAAG
  GCTGCCAGACCTTTCTGAGTGCCCCCAGGGAGGGGCTGGGGCGTCTCATGGTGCCCACTG
  GCGAGGGAGCTGGCATCTCCACCCGCAGTCCTCGCCCCTTCAATGAGATCCCCTCTCCTG
  GTGACAATGGCTGGCTAAACCTGTACCATTTCTGGAGGGAGACGGGCACACACAAAGTCC
  ACCTTCACCATGTCCAGAATTTCCAGAAGTATGGCCCGATTTACAGGGAGAAGCTCGGCA
  ACGTGGAGTCGGTTTATGTCATCGACCCTGAAGATGTGGCCCTTCTCTTTAAGTCCGAGG
  GCCCCAACCCAGAACGATTCCTCATCCCGCCCTGNGTCGCCTATCACCAGTATTACCAGA
  GACCCATAGGAGTCCTGTTGAAGAAGTCGGCAGCCTGGAAGAAAGACCGGGTGGCCCTGA
  ACCAGGAGGTGATGGCTCCAGAGGCCACCAAGAACTTTTTGCCCCTGTTGGATGCAGTGT
  CTCGGGACTTCGTCAGTGTCCTGCACAGGCGCATCAAGATAGCGGGCTCCGGATATTACT
  CGGNGGACATCAGTGATGACCTGTTCCGCTNTGCCTTTGAGTCCATCACTAACGTCATTN
  TTGGGGAGCGCCAGGGGATGCTGGNAGAAGTANTGAACCCCGAGGCCAGCGATTCATTGA
  TGCCATCTACCAGATGTTNCACACCAGCGTNCCCATGCTCAACCTTCCCCAGACCCTGTN
  NCGTCTGTCAGGACCAGACCTGNAATGACATGNTGCTGCATGGGNACGTGATTTCAGTAA
  GCTGACTATACACCAGAACTCTACTGGGAATTGAGACGAAGGGANTGTCACACGATACCG
  TGGCATCTCTAGACTCTGGAGAAGCAGATGTCTTCAGGACTAAGGCACGNCANAATCTGC
  GNNAGGTGGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000781 unedited
  NCGCATTGTCACTGTTTTTGTCTTCTGGGGTGGGTNGAANAGAGTGGCCCAGCTGAGCTG
  AGGAAGGTGACCACTGNANAACCCATTCAACCTGCTGAGCAGGCTGGGCAGAAAGGAGCA
  GGACTTGGGACAGACGACTGAAGATGCAGAGACCCCATGGGCCCCACCCCTGGGCCTTCC
  TCCCATGTGGCTGCAGGCCATCCTCTCTGATCACTGCTGGGTTGCTTCCTGGTTAAAGGG
  CCAGAAGGTGAAGGAGATGGGCTTTTCAGGCATCAGAATGAGGTTGAATGTGGTGCCCAC
  ATCGCTGAGGTGTTGGATTTCAACTCTGAAGTTCTCCAGCATATTGATGAGGAAGATGGT
  CATCTCTAGCTCAGCGATCCGCCGTCCCAGACACTGCCGCACACCCCAGCCAAAGCCCAA
  GTTCCGGAAGTAGGTGATGTTCTTGTCTTTGCTCAGCCATCGGGTTGGGTCAAAATTTTC
  CGGGTCGAAGAAGAAGGTGGGCTCTCGGCCCAGAGCATAGATGGCCACTTGCACCAGTGT
  CTTGGCAGGAATCATGTAATCTCGAAGAACCAAGTCATTTACAAGATATCTCTGCAGGGT
  CACGGAGATGGGGTGAAGTCTTAGTGTCTCCTTGATGCTGGCTTTGAGGAGGGGGACCAG
  CTGTAGCATCGTGGCCATGTCTCCCTGGGCCTGGTGCCGCGCAGCCAAGACCTCTGCCCG
  CAGCATATCCTGCACCTTCAGGTTGCGTGCCATCTCATACAAGTGCCACTGCAGGGTCAT
  GGACGTCGTGTCCACCCCTTCTGCCAGCATCTCTGTGACCGTGGCCTTGATGTCCTCGAA
  GACATTTTGCTGTCTCCCAGNAGTCTGTAGAGGATGCCANCGGTAATCGNGGTGAACACT
  TCCTTTCTGTCTCATTNCCAGTAGAAGTCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000781
• Insert Size: 1840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000781.1, NP_000772.1
• RefSeq Size: 1821 bp
• RefSeq ORF: 1566 bp
• Locus ID: 1583
• UniProt ID: P05108
• Cytogenetics: 15q24.1
• Domains: p450
• Protein Families: Druggable Genome, P450
• Protein Pathways: C21-Steroid hormone metabolism, Metabolic pathways
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (a).