GRIA1 (NM_000827) Human Untagged Clone

CAT#: SC119638

GRIA1 (untagged)-Human glutamate receptor, ionotropic, AMPA 1 (GRIA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRIA1
• Synonyms: GluA1; GLUH1; GLUR1; GLURA; HBGR1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000827 edited
  GAATTCGGCACGAGGGGGAAACAGACAAACCTCACGAAAGGAAGGAAGCAAGCAAGCAAG
  GAAGGAACTGCAGGAGGAAAAGAACAGGCAGAACAGCGAGAAGAATAAAGGGAAAGGGGG
  GGAAACACCAAATCTATGATTGGACCTGGGCTTCTTTTTCGCCAATGCAAAAAGGAATAT
  GCAGCACATTTTTGCCTTCTTCTGCACCGGTTTCCTAGGCGCGGTAGTAGGTGCCAATTT
  CCCCAACAATATCCAGATCGGGGGATTATTTCCAAACCAGCAGTCACAGGAACATGCTGC
  TTTTAGATTTGCTTTGTCGCAACTCACAGAGCCCCCGAAGCTGCTCCCCCAGATTGATAT
  TGTGAACATCAGCGACAGCTTTGAGATGACCTATAGATTCTGTTCCCAGTTCTCCAAAGG
  AGTCTATGCCATCTTTGGGTTTTATGAACGTAGGACTGTCAACATGCTGACCTCCTTTTG
  TGGGGCCCTCCACGTCTGCTTCATTACGCCGAGCTTTCCCGTTGATACATCCAATCAGTT
  TGTCCTTCAGCTGCGCCCTGAACTGCAGGATGCCCTCATCAGCATCATTGACCATTACAA
  GTGGCAGAAATTTGTCTACATTTATGATGCCGACCGGGGCTTATCCGTCCTGCAGAAAGT
  CCTGGATACAGCTGCTGAGAAGAACTGGCAGGTGACAGCAGTCAACATTTTGACAACCAC
  AGAGGAGGGATACCGGATGCTCTTTCAGGACCTGGAGAAGAAAAAGGAGCGGCTGGTGGT
  GGTGGACTGTGAATCAGAACGCCTCAATGCTATCTTGGGCCAGATTATAAAGCTAGAGAA
  GAATGGCATCGGCTACCACTACATTCTTGCAAATCTGGGCTTCATGGACATTGACTTAAA
  CAAATTCAAGGAGAGTGGCGCCAATGTGACAGGTTTCCAGCTGGTGAACTACACAGACAC
  TATTCCGGCCAAGATCATGCAGCAGTGGAAGAATAGTGATGCTCGAGACCACACACGGGT
  GGACTGGAAGAGACCCAAGTACACCTCTGCGCTCACCTACGATGGGGTGAAGGTGATGGC
  TGAGGCTTTCCAGAGCCTGCGGAGGCAGAGAATTGATATATCTCGCCGGGGGAATGCTGG
  GGATTGTCTGGCTAACCCAGCTGTTCCCTGGGGCCAAGGGATCGACATCCAGAGAGCTCT
  GCAGCAGGTGCGATTTGAAGGTTTAACAGGAAACGTGCAGTTTAATGAGAAAGGACGCCG
  GACCAACTACACGCTCCACGTGATTGAAATGAAACATGACGGCATCCGAAAGATTGGTTA
  CTGGAATGAAGATGATAAGTTTGTCCCTGCAGCCACCGATGCCCAAGCTGGGGGCGATAA
  TTCAAGTGTTCAGAACAGAACATACATCGTCACAACAATCCTAGAAGATCCTTATGTGAT
  GCTCAAGAAGAACGCCAATCAGTTTGAGGGCAATGACCGTTACGAGGGCTACTGTGTAGA
  GCTGGCGGCAGAGATTGCCAAGCACGTGGGCTACTCCTACCGTCTGGAGATTGTCAGTGA
  TGGAAAATACGGAGCCCGAGACCCTGACACGAAGGCCTGGAATGGCATGGTGGGAGAGCT
  GGTCTATGGAAGAGCAGATGTGGCTGTGGCTCCCTTAACTATCACTTTGGTCCGGGAAGA
  AGTTATAGATTTCTCCAAACCATTTATGAGTTTGGGGATCTCCATCATGATTAAAAAACC
  ACAGAAATCCAAGCCGGGTGTCTTCTCCTTCCTTGATCCTTTGGCTTATGAGATTTGGAT
  GTGCATTGTTTTTGCCTACATTGGAGTGAGTGTTGTCCTCTTCCTGGTCAGCCGCTTCAG
  TCCCTATGAATGGCACAGTGAAGAGTTTGAGGAAGGACGGGACCAGACAACCAGTGACCA
  GTCCAATGAGTTTGGGATATTCAACAGTTTGTGGTTCTCCCTGGGAGCCTTCATGCAGCA
  AGGATGTGACATTTCTCCCAGGTCCCTGTCTGGTCGCATCGTTGGTGGCGTCTGGTGGTT
  CTTCACCTTAATCATCATCTCCTCATATACAGCCAATCTGGCCGCCTTCCTGACCGTGGA
  GAGGATGGTGTCTCCCATTGAGAGTGCAGAGGACCTAGCGAAGCAGACAGAAATTGCCTA
  CGGGACGCTGGAAGCAGGATCTACTAAGGAGTTCTTCAGGAGGTCTAAAATTGCTGTGTT
  TGAGAAGATGTGGACATACATGAAGTCAGCAGAGCCATCAGTTTTTGTGCGGACCACAGA
  GGAGGGGATGATTCGAGTGAGGAAATCCAAAGGCAAATATGCCTACCTCCTGGAGTCCAC
  CATGAATGAGTACATTGAGCAGCGGAAACCCTGTGACACCATGAAGGTGGGAGGTAACTT
  GGATTCCAAAGGCTATGGCATTGCAACACCCAAGGGGTCTGCCCTGAGAAATCCAGTAAA
  CCTGGCAGTGTTAAAACTGAACGAGCAGGGGCTTTTGGACAAATTGAAAAACAAATGGTG
  GTACGACAAGGGCGAGTGCGGCAGCGGGGGAGGTGATTCCAAGGACAAGACAAGCGCTCT
  GAGCCTCAGCAATGTGGCAGGCGTGTTCTACATCCTGATCGGAGGACTTGGACTAGCCAT
  GCTGGTTGCCTTAATCGAGTTCTGCTACAAATCCCGTAGTGAATCCAAGCGGATGAAGGG
  TTTTTGTTTGATCCCACAGCAATCCATCAACGAAGCCATACGGACATCGACCCTCCCCCG
  CAACAGCGGGGCAGGAGCCAGCAGCGGCGGCAGTGGAGAGAATGGTCGGGTGGTCAGCCA
  TGACTTCCCCAAGTCCATGCAATCGATTCCTTGCATGAGCCACAGTTCAGGGATGCCCTT
  GGGAGCCACGGGATTGTAACTGGAGCAGATGGAGACCCCTTGGGGAGCAGGCTCGGGCTC
  CCCAGCCCCATCCCAAACCCTTCAGTGCCAAAAACAACAACAAAATGAAACGCAACCACC
  ACCAACCACTGCGACCACAAGAAGGATGATTCAACAGGTTTTCCTGAAGAATTGAAAAAC
  CATTTTGCTGTCCCTTTTCCTTTTTTGATGTTCTTTCACCCTTTTCTGTTTGCTAAGTGA
  GGATGAAAAAATAACACTGTACTGCAATAAGGGGAGAGTAACCCTGTCTAATGAAACCTG
  TGTCTCTGAGAGTAGAGTCACTGGAACACTAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000827 unedited
  ACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGNGAAACAGACAAACCTCACGAAA
  GGAAGGAAGCAAGCAAGCAAGGAAGGAACTGCAGGAGGAAAAGAACAGGCAGAACAGCGA
  GAAGAATAAAGGGAAAGGGGGGGAAACACCAAATCTATGATTGGACCTGGGCTTCTTTTT
  CGCCAATGCAAAAAGGAATATGCAGCACATTTTTGCCTTCTTCTGCACCGGTTTCCTAGG
  CGCGGTAGTAGGTGCCAATTTCCCCAACAATATCCAGATCGGGGGATTATTTCCAAACCA
  GCAGTCACAGGAACATGCTGCTTTTAGATTTGCTTTGTCGCAACTCACAGAGCCCCCGAA
  GCTGCTCCCCCAGATTGATATTGTGAACATCAGCGACAGCTTTGAGATGACCTATAGATT
  CTGTTCCCAGTTCTCCAAAGGAGTCTATGCCATCTTTGGGTTTTATGAACGTAGGACTGT
  CAACATGCTGACCTCCTTTTGTGGGGCCCTCCACGTCTGCTTCATTACGCCGAGCTTTCC
  CGTTGATACATCCAATCAGTTTGTCCTTCAGCTGCGCCCTGAACTGCAGGATGCCCTCAT
  CAGCATCATTGACCATTACAAGTGGCAGAAATTTGTCTACATTTATGATGCCGACCGGNG
  CTTATCCGTCCTGCAGAAAGTCCTGNATACAGCTGCTGAGAAGACTGGCANGTGACAGCA
  GTCAACATTTTGACAACCACAGAGGANGGATACCGNATGCTCTTTCANGACCTGNAGAAA
  AAAAGAGCNNGCTGTGGTGGTGGACTGTGAATCAAACGCCCTCATGCTATCTGGGCCAGA
  TATAAGCTAGAGAGAATGGCATCGCTACACTACATTCTGCAAATCTGGCTCATGGACATT
  GACTAACAANTCANGAAGTGCCGCATGTGGAAAGNTNCAGCTNTGAACTAACAGACTATT
  NCGNCAATCATGCACATGAAAATATGAGCTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000827 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGTGTTCCAGTGACTC
  TACTCTCAGAGAACAGGTTTCATTAGACAGGGTTACTCTCCCCTTATTGCAGTACAGTGT
  TATTTTTTCATCCTCACTTAGCAAACAGAAAAGGGTGAAAGAACATCAAAAAAGGAAAAG
  GGACAGCAAAATGGTTTTTCAATTCTTCAGGAAAACCTGTTGAATCATCCTTCTTGTGGT
  CGCAGTGGTTGGTGGTGGTTGCGTTTCATTTTGTTGTTGTTTTTGGCACTGAAGGGTTTG
  GGATGGGGCTGGGGAGCCCGAGCCTGCTCCCCAAGGGGTCTCCATCTGCTCCAGTTACAA
  TCCCGTGGCTCCCAAGGGCATCCCTGAACTGTGGCTCATGCAAGGAATCGATTGCATGGA
  CTTGGGGAAGTCATGGCTGACCACCCGACCATTCTCTCCACTGCCGCCGCTGCTGGCTCC
  TGCCCCGCTGTTGCGGGGGAGGGTCGATGTCCGTATGGCTTCGTTGATGGATTGCTGTGG
  GATCAAACAAAAACCCTTCATCCGCTTGGATTCACTACGGGATTTGTAGCAGAACTCGAT
  TAAGGCAACCAGCATGGCTAGTCCAAGTCCTCCGATCAGGATGTAGAACACGCCTGCCAC
  ATTGCTGAGGCTCAGAGCGCTTGTCTTGTCCTTGGGATCACCTNCCCCGCTGCCGCACTC
  GCCCTTGTCGTACACCATTTGTTTTTCAATTTGTCCAAAAGCCCCTGCTCGTTCAGTTTA
  ACACTGCCAGTTTACTGGATTCTCAGGCAGACCCCTTGNNGTGTGCAATGCATAGCCTTT
  GGAATCCAGTTACCTNCCACCTTCATGTGTCACAAGGTTTCCGCTGCTCATGTACTCATC
  ATGGNGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000827
• Insert Size: 3510 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000827.2, NP_000818.1
• RefSeq Size: 3242 bp
• RefSeq ORF: 2721 bp
• Locus ID: 2890
• UniProt ID: P42261
• Cytogenetics: 5q33.2
• Domains: lig_chan, ANF_receptor
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Long-term depression, Long-term potentiation, Neuroactive ligand-receptor interaction
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes isoform 1.