GGCX (NM_000821) Human Untagged Clone

CAT#: SC119634

GGCX (untagged)-Human gamma-glutamyl carboxylase (GGCX), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GGCX
• Synonyms: VKCFD1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119634 sequence for NM_000821 edited (data generated by NextGen Sequencing)
  ATGGCGGTGTCTGCCGGGTCCGCGCGGACCTCGCCCAGCTCAGATAAAGTACAGAAAGAC
  AAGGCTGAACTGATCTCAGGGCCCAGGCAGGACAGCCGAATAGGGAAACTCTTGGGTTTT
  GAGTGGACAGATTTGTCCAGTTGGCGGAGGCTGGTGACCCTGCTGAATCGACCAACGGAC
  CCTGCAAGCTTAGCTGTCTTTCGTTTTCTTTTTGGGTTCTTGATGGTGCTAGACATTCCC
  CAGGAGCGGGGGCTCAGCTCTCTGGACCGGAAATACCTTGATGGGCTGGATGTGTGCCGC
  TTCCCCTTGCTGGATGCCCTACGCCCACTGCCACTTGACTGGATGTATCTTGTCTACACC
  ATCATGTTTCTGGGGGCACTGGGCATGATGCTGGGCCTGTGCTACCGGATAAGCTGTGTG
  TTATTCCTGCTGCCATACTGGTATGTGTTTCTCCTGGACAAGACATCATGGAACAACCAC
  TCCTATCTGTATGGGTTGTTGGCCTTTCAGCTAACATTCATGGATGCAAACCACTACTGG
  TCTGTGGACGGTCTGCTGAATGCCCATAGGAGGAATGCCCACGTGCCCCTTTGGAACTAT
  GCAGTGCTCCGTGGCCAGATCTTCATTGTGTACTTCATTGCGGGTGTGAAAAAGCTGGAT
  GCAGACTGGGTTGAAGGCTATTCCATGGAATATTTGTCCCGGCACTGGCTCTTCAGTCCC
  TTCAAACTGCTGTTGTCTGAGGAGCTGACTAGCCTGCTGGTCGTGCACTGGGGTGGGCTG
  CTGCTTGACCTCTCAGCTGGTTTCCTGCTCTTTTTTGATGTCTCAAGATCCATTGGCCTG
  TTCTTTGTGTCCTACTTCCACTGCATGAATTCCCAGCTTTTCAGCATTGGTATGTTCTCC
  TACGTCATGCTGGCCAGCAGCCCTCTCTTCTGCTCCCCTGAGTGGCCTCGGAAGCTGGTG
  TCCTACTGCCCCCAAAGGTTGCAACAACTGTTGCCCCTCAAGGCAGCCCCTCAGCCCAGT
  GTTTCCTGTGTGTATAAGAGGAGCCGGGGCAAAAGTGGCCAGAAGCCAGGGCTGCGCCAT
  CAGCTGGGAGCTGCCTTCACCCTGCTCTACCTCCTGGAGCAGCTATTCCTGCCCTATTCT
  CATTTTCTCACCCAGGGCTATAACAACTGGACAAATGGGCTGTATGGCTATTCCTGGGAC
  ATGATGGTGCACTCCCGTTCCCACCAGCACGTGAAGATCACCTACCGTGATGGCCGCACT
  GGCGAACTGGGCTACCTTAACCCTGGGGTATTTACACAGAGTCGGCGATGGAAGGATCAT
  GCAGACATGCTGAAGCAATATGCCACTTGCCTGAGCCGCCTGCTTCCCAAGTATAATGTC
  ACTGAGCCCCAGATCTACTTTGATATTTGGGTCTCCATCAATGACCGCTTCCAGCAGAGG
  ATTTTTGACCCTCGTGTGGACATCGTGCAGGCCGCTTGGTCACCCTTTCAGCGCACATCC
  TGGGTGCAACCACTCTTGATGGACCTGTCTCCCTGGAGGGCCAAGTTACAGGAAATCAAG
  AGCAGCCTAGACAACCACACTGAGGTGGTCTTCATTGCAGATTTCCCTGGACTGCACTTG
  GAGAATTTTGTGAGTGAAGACCTGGGCAACACTAGCATCCAGCTGCTGCAGGGGGAAGTG
  ACTGTGGAGCTTGTGGCAGAACAGAAGAACCAGACTCTTCGAGAGGGAGAAAAAATGCAG
  TTGCCTGCTGGTGAGTACCATAAGGTGTATACGACATCACCTAGCCCTTCTTGCTACATG
  TACGTCTATGTCAACACTACAGAGCTTGCACTGGAGCAAGACCTGGCATATCTGCAAGAA
  TTAAAGGAAAAGGTGGAGAATGGAAGTGAAACAGGGCCTCTACCCCCAGAGCTGCAGCCT
  CTGTTGGAAGGGGAAGTAAAAGGGGGCCCTGAGCCAACACCTCTGGTTCAGACCTTTCTT
  AGACGCCAACAAAGGCTCCAGGAGATTGAACGCCGGCGAAATACTCCTTTCCATGAGCGA
  TTCTTCCGCTTCTTGTTGCGAAAGCTCTATGTCTTTCGCCGCAGCTTCCTGATGACTTGT
  ATCTCACTTCGAAATCTGATATTAGGCCGTCCTTCCCTGGAGCAGCTGGCCCAGGAGGTG
  ACTTATGCAAACTTGAGACCCTTTGAGGCAGTTGGAGAACTGAATCCCTCAAACACGGAT
  TCTTCACATTCTAATCCTCCTGAGTCAAATCCTGATCCTGTCCACTCAGAGTTCTGA
  
  Clone variation with respect to NM_000821.5
  974 g=>a;1218 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000821 unedited
  NGTTTCAGCATTTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGCCC
  ACCTGCCTCCTCCGCAGAGCAATGGCGGTGTCTGCCGGGTCCGCGCGGACCTCGCCCAGC
  TCAGATAAAGTACAGAAAGACAAGGCTGAACTGATCTCAGGGCCCAGGCAGGACAGCCGA
  ATAGGGAAACTCTTGGGTTTTGAGTGGACAGATTTGTCCAGTTGGCGGAGGCTGGTGACC
  CTGCTGAATCGACCAACGGACCCTGCAAGCTTAGCTGTCTTTCGTTTTCTTTTTGGGTTC
  TTGATGGTGCTAGACATTCCCCAGGAGCGGGGGCTCAGCTCTCTGGACCGGAAATACCTT
  GATGGGCTGGATGTGTGCCGCTTCCCCTTGCTGGATGCCCTACGCCCACTGCCACTTGAC
  TGGATGTATCTTGTCTACACCATCATGTTTCTGGGGGCACTGGGCATGATGCTGGGCCTG
  TGCTACCGGATAAGCTGTGTGTTATTCCTGCTGCCATACTGGTATGTGTTTCTCCTGGAC
  AAGACATCATGGAACAACCACTCCTATCTGTATGGGTTGTTGGCCTTTCAGCTAACATTC
  ATGGATGCAAACCACTACTGGTCTGTGGACGGTCTGCTGAATGCCCATAGGAGGAATGCC
  CACGTGCCCCTTTGGAACTATGCAGTGCTCCGTGGCCAGATCTTCATTGTGTACTTCATT
  GCGGGTGTGAAAAAGCTGGATGCAGACTGGGTTGAAGCTATTCCATGGAATAATTGTCCC
  GCACTGGCTCTTCAGTCCCTTCAACTGCTGTTGTCTGAAGAGCTGACTANCCTGCTGTTC
  GTGCACTGGGGTGGGCTGCTGCTTGACCTCTCAGCTGGTTTCTGCTCTTTNTTGATGCTC
  AAGACCATTGGCCTGTCTTTGGGCCTACTTCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000821 unedited
  CTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGCCACTTTAT
  TTATTTATTTATTTTGAGACGAAGTCTTGCTCTGTTGCCCAGGAGTGCAGTGGCTCGATC
  TCGGCTCACTGCAACCTCCACCTCCTGGGCCCAAGCGATTCTCATGCCTCAGCCTCCTGG
  GTAGTTGGATTACAGGCACCCACCACCACGTCTGGCTAATTTTTGTATTTTTAGTAGAGA
  AGGGGTCTTGCCATGTTGGCCAGGCTGGTCTCGAACTCCTGGCCTCAAGTGATCTGCCCA
  CCTCAGCCTCCCAAAATGCTGGGATTACAGGCGTGAGCCACGGCACCCAGCCCCCAAAAA
  GCCACTTTAAACCTTATCCTAGGAGGACAGTTTCACATTGCGTCTAACCTCTTCCTGGCC
  TCTTAATCTTGGGTTGTTAAATCTTATTTGCTTTATTTCCTTGGTTCCTCTAAGTTGTAA
  TCTCGGAGTTAAAAACAGCTTTAGAACCCCGCCCCCCCAAAAAAAAAAAAAAAAACTTTT
  GAGAATTTTTTTCAAATAAATGTCCATTGCATAGAATGGGTCTGTGACTGGCTGCTTCTA
  CATCTGCACCCAACATCTGGCCCCCTTCAGAACTCTGAGTGGACAGGATCAGGATTTGAC
  TCANGAGGATTAGAATGTGAAGAATCCGTGTTTGAGGGATTCAGTTCTCCAACTGCCTCA
  AAGGGTCTCAAGTTTGCATAAGTCACCTCCTGGGCCAGCTGCCTCCAGGAAGGACGGCCT
  AATATCAGATTTCGAAGTGAGATACAAGTCATCAGGAACCTGCGGCGAAAGAATAGAGCT
  TTCGCAACAGAAGCGGAGAATCGCTCATGGAAAGGAGTATTTTCGCCGCGTTCATCTCCT
  GGAGCCTTTGTTGCGTCTAAGAAAGTCTGACCAGAGGGTTGCCTCAGGNCCCCCTAACTT
  CCCTCCACAGAGCTGCGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000821
• Insert Size: 2940 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000821.3, NP_000812.2
• RefSeq Size: 3236 bp
• RefSeq ORF: 2277 bp
• Locus ID: 2677
• UniProt ID: P38435
• Cytogenetics: 2p11.2
• Domains: VKG_Carbox
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes an integral membrane protein of the rough endoplasmic reticulum that carboxylates glutamate residues of vitamin K-dependent proteins to gamma carboxyl glutamate, a modification that is required for their activity. The vitamin K-dependent protein substrates have a propeptide that binds the enzyme, with carbon dioxide, dioxide, and reduced vitamin K acting as co-substrates. Vitamin K-dependent proteins affect a number of physiologic processes including blood coagulation, prevention of vascular calcification, and inflammation. Allelic variants of this gene have been associated with pseudoxanthoma elasticum-like disorder with associated multiple coagulation factor deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.