GAD67 (GAD1) (NM_000817) Human Untagged Clone

SKU SC119631

GAD1 (untagged)-Human glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67

Product Data

• Type: Human Untagged Clone
• Target Symbol: GAD67
• Synonyms: CPSQ1; DEE89; GAD; SCP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119631 sequence for NM_000817 edited (data generated by NextGen Sequencing); ATGGCGTCTTCGACCCCATCTTCGTCCGCAACCTCCTCGAACGCGGGAGCGGACCCCAAT; ACCACTAACCTGCGCCCCACAACGTACGATACCTGGTGCGGCGTGGCCCACGGATGCACC; AGAAAACTGGGGCTCAAGATCTGCGGCTTCTTGCAAAGGACCAACAGCCTGGAAGAGAAG; AGTCGCCTTGTGAGTGCCTTCAAGGAGAGGCAATCCTCCAAGAACCTGCTTTCCTGTGAA; AACAGCGACCGGGATGCCCGCTTCCGGCGCACAGAGACTGACTTCTCTAATCTGTTTGCT; AGAGATCTGCTTCCGGCTAAGAACGGTGAGGAGCAAACCGTGCAATTCCTCCTGGAAGTG; GTGGACATACTCCTCAACTATGTCCGCAAGACATTTGATCGCTCCACCAAGGTGCTGGAC; TTTCATCACCCACACCAGTTGCTGGAAGGCATGGAGGGCTTCAACTTGGAGCTCTCTGAC; CACCCCGAGTCCCTGGAGCAGATCCTGGTTGACTGCAGAGACACCTTGAAGTATGGGGTT; CGCACAGGTCATCCTCGATTTTTCAACCAGCTCTCCACTGGATTGGATATTATTGGCCTA; GCTGGAGAATGGCTGACATCAACGGCCAATACCAACATGTTTACATATGAAATTGCACCA; GTGTTTGTCCTCATGGAACAAATAACACTTAAGAAGATGAGAGAGATAGTTGGATGGTCA; AGTAAAGATGGTGATGGGATATTTTCTCCTGGGGGCGCCATATCCAACATGTACAGCATC; ATGGCTGCTCGCTACAAGTACTTCCCGGAAGTTAAGACAAAGGGCATGGCGGCTGTGCCT; AAACTGGTCCTCTTCACCTCAGAACAGAGTCACTATTCCATAAAGAAAGCTGGGGCTGCA; CTTGGCTTTGGAACTGACAATGTGATTTTGATAAAGTGCAATGAAAGGGGGAAAATAATT; CCAGCTGATTTTGAGGCAAAAATTCTTGAAGCCAAACAGAAGGGATATGTTCCCTTTTAT; GTCAATGCAACTGCTGGCACGACTGTTTATGGAGCTTTTGATCCGATACAAGAGATTGCA; GATATATGTGAGAAATATAACCTTTGGTTGCATGTCGATGCTGCCTGGGGAGGTGGGCTG; CTCATGTCCAGGAAGCACCGCCATAAACTCAACGGCATAGAAAGGGCCAACTCAGTCACC; TGGAACCCTCACAAGATGATGGGCGTGCTGTTGCAGTGCTCTGCCATTCTCGTCAAGGAA; AAGGGTATACTCCAAGGATGCAACCAGATGTGTGCAGGATACCTCTTCCAGCCAGACAAG; CAGTATGATGTCTCCTACGACACCGGGGACAAGGCAATTCAGTGTGGCCGCCACGTGGAT; ATCTTCAAGTTCTGGCTGATGTGGAAAGCAAAGGGCACAGTGGGATTTGAAAACCAGATC; AACAAATGCCTGGAACTGGCTGAATACCTCTATGCCAAGATTAAAAACAGAGAAGAATTT; GAGATGGTTTTCAATGGCGAGCCTGAGCACACAAACGTCTGTTTTTGGTATATTCCACAA; AGCCTCAGGGGTGTGCCAGACAGCCCTCAACGACGGGAAAAGCTACACAAGGTGGCTCCA; AAAATCAAAGCCCTGATGATGGAGTCAGGTACGACCATGGTTGGCTACCAGCCCCAAGGG; GACAAGGCCAACTTCTTCCGGATGGTCATCTCCAACCCAGCCGCTACCCAGTCTGACATT; GACTTCCTCATTGAGGAGATAGAAAGACTGGGCCAGGATCTGTAA; ; Clone variation with respect to NM_000817.2; 111 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000817 unedited; GCTCTTGCCCGCCCGTCGCCTCTAAGGGCGGTAGGCGTGTACGGTGGGNAGGTCTATATA; AGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCG; CGAATTCGGCACGAGGCGCGTGCGAGAGGGAACTAGCGAGAACGAGGAAGCAGCTGGAGG; TGACGCCGGGCAGATTACGCCTGTCAGGGCCGAGCCGAGCGGATCGCTGGGCGCTGTGCA; GAGGAAAGGCGGGAGTGCCCGGCTCGCTGTCGCAGAGCCGAGCCTGTTTCTGCGCCGGAC; CAGTCGAGGACTCTGGACAGTAGAGGCCCCGGGACGACCGAGCTGATGGCGTCTTCGACC; CCATCTTCGTCCGCAACCTCCTCGAACGCGGGAGCGGACCCCAATACCACTAACCTGCGC; CCCACAACGTACGATACCTGGTGCGGCGTGGCCCACGGATGCACCAGAAAACTGGGGCTC; AAGATCTGCGGCTTCTTGCAAAGGACCAACAGCCTGGAAGAGAAGAGTCGCCTTGTGAGT; GCCTTCAAGGAGAGGCAATCCTCCAAGAACCTGCTTTCCTGTGAAAACAGCGACCGGGAT; GCCCGCTTCCGGCGCACAGAGACTGACTTCTCTAATCTGTTTGCTAGAGATCTGCTTCCG; GCTAAGAACGGTGAGGAGCAAACCGTGCAATTCCTCCTGGAAGTGGTGGACATACTCCTC; AACTATGTCCGCAAGACATTTGATCGCTCCACCAAGGTGCTGGACTTTCATCACCCACAC; CAGTTGCTGGAAGGCATGGGAGGCTTCAACTTGGAGCTCTCTGACCACCCCGAGTCCCTG; GAGCAGATCCTGGTTGACTGCAGAGACACCTTGAAGTATGGAGTTCGCACAGGTCATCCT; CGATTTTTCAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000817 unedited; CGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGTTCAAATTCTCCATGGAGA; ATATTTTTATTTATTTATATTCTTCTTGCATACTCTAAAACGCACAGTGTGAATTTCCCT; TCTTCAAAAACAGTTGTGAGCCTGGTCACTTTATCTGAGATCTTCAGAAGGTCTTCGGAA; ATGTTGCCTTAGGTTTCAGCTAAGCGAGTCACAGAGATTGGTCATCAACTACTACAAAAT; TACAGGAGATCTAGTTATTATCCAACAGTTGCTAGAAAAGCATTGAGAACACCAGACGTA; CAGCCAGACAGAGAGCGTAAGAGAAGAGCAGATGCTATTTAAGTAACAGGATTTCCATAT; ATATGACTTTATGTACAATCTTAGAATTCTCTTACAGACAGTAAAATGCTTAAATCATTA; TTTTAAGGGCACTCTAATATAAATACAAATCTCACTAATTTTAATCTTTTCTCTTTGGCT; CACAAATGCACGAAAATACCATAAACAGTTTTCAGAACTTACTTCATATATAAATAAGAT; ACATTACAAATGGGAAGAAAATACAAGACGCCTCTTTAACATGGTACATCTCTGAATATA; AATAAGCAAATATTTCATTGACCTACAATTAAACAATCATTGAAATCATGACACATAGTC; TCCCCCTCCCACCACCAAGGTCAGACAACAGGAATTTACACAATCTACACAAATTAATCT; CTAAAAATACAGCCATAGTACACCTGGGTACATGACTCTGCTACTATTTTCCCCTACAGG; ATACTAATGGGGNAGGGNTGTGACTGTCATTGGNNGGNNGTTTTTCTCATCCATCATCAC; TTCAGGTGGAGAAACGCTGACAGTGAGGTCGATTCTTACCCAACAGCATTCCTTTGGCCN; NCTAGACATGGCGTTTGAAAAACAGTTNCCTTGGAGTGTTGACAANCTGGNTGGCNCATA; GTTANCTCCTCCGTAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_000817
• Insert Size: 3270 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000817.2, NP_000808.2
• RefSeq Size: 3488 bp
• RefSeq ORF: 1785 bp
• Locus ID: 2571
• UniProt ID: Q99259
• Cytogenetics: 2q31.1
• Domains: pyridoxal_deC
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, beta-Alanine metabolism, Butanoate metabolism, Metabolic pathways, Taurine and hypotaurine metabolism, Type I diabetes mellitus
• Summary: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]; Transcript Variant: Transcript variant GAD67 represents the full-length and predominant form of this gene.