SCF (KITLG) (NM_000899) Human Untagged Clone

CAT#: SC119596

KITLG (untagged)-Human KIT ligand (KITLG), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SCF
• Synonyms: DCUA; DFNA69; FPH2; FPHH; Kitl; KL-1; MGF; SCF; SF; SHEP7; SLF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119596 sequence for NM_000899 edited (data generated by NextGen Sequencing)
  ATGAAGAAGACACAAACTTGGATTCTCACTTGCATTTATCTTCAGCTGCTCCTATTTAAT
  CCTCTCGTCAAAACTGAAGGGATCTGCAGGAATCGTGTGACTAATAATGTAAAAGACGTC
  ACTAAATTGGTGGCAAATCTTCCAAAAGACTACATGATAACCCTCAAATATGTCCCCGGG
  ATGGATGTTTTGCCAAGTCATTGTTGGATAAGCGAGATGGTAGTACAATTGTCAGACAGC
  TTGACTGATCTTCTGGACAAGTTTTCAAATATTTCTGAAGGCTTGAGTAATTATTCCATC
  ATAGACAAACTTGTGAATATAGTGGATGACCTTGTGGAGTGCGTGAAAGAAAACTCATCT
  AAGGATCTAAAAAAATCATTCAAGAGCCCAGAACCCAGGCTCTTTACTCCTGAAGAATTC
  TTTAGAATTTTTAATAGATCCATTGATGCCTTCAAGGACTTTGTAGTGGCATCTGAAACT
  AGTGATTGTGTGGTTTCTTCAACATTAAGTCCTGAGAAAGATTCCAGAGTCAGTGTCACA
  AAACCATTTATGTTACCCCCTGTTGCAGCCAGCTCCCTTAGGAATGACAGCAGTAGCAGT
  AATAGGAAGGCCAAAAATCCCCCTGGAGACTCCAGCCTACACTGGGCAGCCATGGCATTG
  CCAGCATTGTTTTCTCTTATAATTGGCTTTGCTTTTGGAGCCTTATACTGGAAGAAGAGA
  CAGCCAAGTCTTACAAGGGCAGTTGAAAATATACAAATTAATGAAGAGGATAATGAGATA
  AGTATGTTGCAAGAGAAAGAGAGAGAGTTTCAAGAAGTGTAA
  
  Clone variation with respect to NM_000899.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000899 unedited
  GGGTGCAAATTATGTATACGACTCACTTAGGCGGCCGCGAAATTCGCACGAGGGAACAGC
  TAAACGGAGTCGCCACACCACTGTTTGTGCTGGATCGCAGCGCTGCCTTTCCTTATGAAG
  AAGACACAAACTTGGATTCTCACTTGCATTTATCTTCAGCTGCTCCTATTTAATCCTCTC
  GTCAAAACTGAAGGGATCTGCAGGAATCGTGTGACTAATAATGTAAAAGACGTCACTAAA
  TTGGTGGCAAATCTTCCAAAAGACTACATGATAACCCTCAAATATGTCCCCGGGATGGAT
  GTTTTGCCAAGTCATTGTTGGATAAGCGAGATGGTAGTACAATTGTCAGACAGCTTGACT
  GATCTTCTGGACAAGTTTTCAAATATTTCTGAAGGCTTGAGTAATTATTCCATCATAGAC
  AAACTTGTGAATATAGTGGATGACCTTGTGGAGTGCGTGAAAGAAAACTCATCTAAGGAT
  CTAAAAAAATCATTCAAGAGCCCAGAACCCAGGCTCTTTACTCCTGAAGAATTCTTTAGA
  ATTTTTAATAGATCCATTGATGCCTTCAAGGACTTTGTAGTGGCATCTGAAACTAGTGAT
  TGTGTGGTTTCTTCAACATTAAGTCCTGAGAAAGATTCCAGAGTCAGTGTCACAAAACCA
  TTTATGTTACCCCCTGTTGCAGCCAGCTCCCTTANGAATGACAGCAGTAGCAGTAATAGG
  AAGGCCAAAAATCCCCCTGGAGACTCCAGCCTACACTGNGCAGCCATGGCATTGCCAGCA
  TTGTTTTCTCTTATAATTGGCTNTGCTTTTGGAGCCCTATACTTGGAGAAGAGACAGCCA
  AGTCTTACAAGGGCAGTTGAAATATACCAATAATGAAGAGGATATGAGATAAGTTGTTGC
  AAGAAAAGAGAGAGAGTTCAAGAGTGTTATGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000899 unedited
  GGTTTAACTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTGAGAGTGCATAAAATTCTTTATTTAGAACAATAAAGTATATGTATATAT
  CATGTAATTTTAAATTCAAACCTGTATAAAAAAGGGTTGGGACATACACCTATTTTAAAA
  AGTACACATACCGTATATACACATACCCATCACATTTTACAACCTTTTTTATTTAATTAA
  ATTTCAGTCATAATAGAACTATTTAAAATAGTGTCGACATACATTTACATTATTATATGC
  AAACTCCAATCTATGACTGAACTGAGGGTATATCTGCGCATCCATCTAAATTAATACTTT
  TCAAGTCCATTGATTTAAAAAAATGGTGGCAAGTGGACACTATAATCACATATTAACCCT
  TAGAATATAGAATAGAATATTTTGATTGGAAAATTATATTTAGTGAGTATTGTAGCTCAA
  AGATCAAGTACTGGAAAACGGTGTGGTTTTTAGTTTAACAGTTGTTTTAATTTTATTGTT
  AAAAACATCATAAGGATGTAGTTGGAGATGGCAGTTATAAATAAATTTACAATGCTTAGT
  AAACATTTCAGGATCACAAATATCCATTTTAGCATGGATTATTTCTTGAAAAAAACACCT
  TGTTATAAAATGAAAGTACCATTATTTTATTTTTTACAACAAAAGCATTTCAAGACTAGT
  TAATGGGAAGCAGCACCTTATGAATTGGTGATCAAACTCACCAATGAACTATTAGAATTT
  CAATCCGAATGTTTTATTTTAAAGCCCATTTCAAATAATATTGTTTCAAAGAAAAACTGG
  TTACTTTAAATTCTTAAACCCTTTCCCATTTTAAAGGGAATTTAAGAAATCACCCTTTTA
  AATGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000899
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000899.3, NP_000890.1
• RefSeq Size: 5435 bp
• RefSeq ORF: 822 bp
• Locus ID: 4254
• UniProt ID: P21583
• Cytogenetics: 12q21.32
• Domains: SCF
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer
• Gene Summary: This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (b) encodes the longer isoform (b) which contains the primary proteolytic-cleavage site; as a result, isoform b is largely a soluble product. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.