Prostaglandin D Synthase (PTGDS) (NM_000954) Human Untagged Clone

CAT#: SC119558

PTGDS (untagged)-Human prostaglandin D2 synthase 21kDa (brain) (PTGDS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Prostaglandin D Synthase
• Synonyms: L-PGDS; LPGDS; PDS; PGD2; PGDS; PGDS2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119558 sequence for NM_000954 edited (data generated by NextGen Sequencing)
  ATGGCTACTCATCACACGCTGTGGATGGGACTGGCCCTGCTGGGGGTGCTGGGCGACCTG
  CAGGCAGCACCGGAGGCCCAGGTCTCCGTGCAGCCCAACTTCCAGCAGGACAAGTTCCTG
  GGGCGCTGGTTCAGCGCGGGCCTCGCCTCCAACTCGAGCTGGCTCCGGGAGAAGAAGGCG
  GCGTTGTCCATGTGCAAGTCTGTGGTGGCCCCTGCCACGGATGGTGGCCTCAACCTGACC
  TCCACCTTCCTCAGGAAAAACCAGTGTGAGACCCGAACCATGCTGCTGCAGCCCGCGGGG
  TCCCTCGGCTCCTACAGCTACCGGAGTCCCCACTGGGGCAGCACCTACTCCGTGTCAGTG
  GTGGAGACCGACTACGACCAGTACGCGCTGCTGTACAGCCAGGGCAGCAAGGGCCCTGGC
  GAGGACTTCCGCATGGCCACCCTCTACAGCCGAACCCAGACCCCCAGGGCTGAGTTAAAG
  GAGAAATTCACCGCCTTCTGCAAGGCCCAGGGCTTCACAGAGGATACCATTGTCTTCCTG
  CCCCAAACCGATAAGTGCATGACGGAACAATAG
  
  Clone variation with respect to NM_000954.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000954 unedited
  TGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGCTCGCTCTCCCACACC
  ACTGGCACCAGGCCCCGGACACCCGCTCTGCTGCAGGAGAATGGCTACTCATCACACGCT
  GTGGATGGGACTGGCCCTGCTGGGGGTGCTGGGCGACCTGCAGGCAGCACCGGAGGCCCA
  GGTCTCCGTGCAGCCCAACTTCCAGCAGGACAAGTTCCTGGGGCGCTGGTTCAGCGCGGG
  CCTCGCCTCCAACTCGAGCTGGCTCCGGGAGAAGAAGGCGGCGTTGTCCATGTGCAAGTC
  TGTGGTGGCCCCTGCCACGGATGGTGGCCTCAACCTGACCTCCACCTTCCTCAGGAAAAA
  CCAGTGTGAGACCCGAACCATGCTGCTGCAGCCCGCGGGGTCCCTCGGCTCCTACAGCTA
  CCGGAGTCCCCACTGGGGCAGCACCTACTCCGTGTCAGTGGTGGAGACCGACTACGACCA
  GTACGCGCTGCTGTACAGCCAGGGCAGCAAGGGCCCTGGCGAGGACTTCCGCATGGCCAC
  CCTCTACAGCCGAACCCAGACCCCCAGGGCTGAGTTAAAGGAGAAATTCACCGCCTTCTG
  CAAGGCCCAGGGCTTCACAGAGGATACCATTGTCTTCCTGCCCCAAACCGATAAGTGCAT
  GACGGAACAATAGGACTCCCCAGGGCTGAAGCTGGGATCCCGGCCAGCCCAGTGACCCCC
  ACGCTCTGGATGTCTCTGCTCTGGTTCCTTTCCCCGAAGCCCTGCCCCCGCTNCCCGCCA
  AAGCACCCCTGCCCCACTCGGGCTTTATCCTGCACANATAAACTCCGGAAGCAGTCAAAA
  AAAAAAAAAAAAACTCGACTCTAGATTGGGGCCGGGTCATAGCTGTTCCTGAACAGATCC
  GGNTGGCATCCTGGGACCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000954
• Insert Size: 1880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000954.5, NP_000945.3
• RefSeq Size: 837 bp
• RefSeq ORF: 573 bp
• Locus ID: 5730
• UniProt ID: P41222
• Cytogenetics: 9q34.3
• Domains: lipocalin
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways
• Gene Summary: The protein encoded by this gene is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of prostaglandin H2 (PGH2) to postaglandin D2 (PGD2). PGD2 functions as a neuromodulator as well as a trophic factor in the central nervous system. PGD2 is also involved in smooth muscle contraction/relaxation and is a potent inhibitor of platelet aggregation. This gene is preferentially expressed in brain. Studies with transgenic mice overexpressing this gene suggest that this gene may be also involved in the regulation of non-rapid eye movement sleep. [provided by RefSeq, Jul 2008]