CD13 (ANPEP) (NM_001150) Human Untagged Clone

CAT#: SC119422

ANPEP (untagged)-Human alanyl (membrane) aminopeptidase (ANPEP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD13
• Synonyms: APN; CD13; GP150; LAP1; P150; PEPN
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001150 edited
  GAATTCGGCACGAGCCAGTGCTGGAGGGGCAGGGACGGCGGCGGCGCAGCTCGGAACCCG
  CCAGGGTCCAGGGTCCAGGTTCCAGCGCCCGGCGGCCCAGCTCTCAAGCAGATCAATGCA
  ATGCCACCTGGCCGCTTGCTTTGCCCACTGGGCCTACAGCCGGAAGCCTGCCCTTCAGCC
  CTCGGGCCTGATCCCAGGCCGCCTGCAGCCTGTAACCAGACACTGTTTGCTTCCAGCAGG
  CACCCCCCGAGCCCAGCTCCACACACCGTTCCTGGATCTCCTCTCCCCAGGCGGAGCGTG
  CCCCTGCCCAGTCCAGCGACCTTCGCCTGTTGGAGCCCTGGTTAATTTTTGCCCAGTCTG
  CCTGTTGTGGGGCTCCTCCCCTTTGGGGATATAAGCCCGGCCTGGGGCTGCTCCGTTCTC
  TGCCTGGCCTGAGGCTCCCTGAGCCGCCTCCCCACCATCACCATGGCCAAGGGCTTCTAT
  ATTTCCAAGTCCCTGGGCATCCTGGGGATCCTCCTGGGCGTGGCAGCCGTGTGCACAATC
  ATCGCACTGTCAGTGGTGTACTCCCAGGAGAAGAACAAGAACGCCAACAGCTCCCCCGTG
  GCCTCCACCACCCCGTCCGCCTCAGCCACCACCAACCCCGCCTCGGCCACCACCTTGGAC
  CAAAGTAAAGCGTGGAATCGTTACCGCCTCCCCAACACGCTGAAACCCGATTCCTACCAG
  GTGACGCTGAGACCGTACCTCACCCCCAATGACAGGGGCCTGTACGTTTTTAAGGGCTCC
  AGCACCGTCCGTTTCACCTGCAAGGAGGCCACTGATGTCATCATCATCCACAGCAAGAAG
  CTCAACTACACCCTCAGCCAGGGGCACAGGGTGGTCCTGCGTGGTGTGGGAGGCTCCCAG
  CCCCCCGACATTGACAAGACTGAGCTGGTGGAGCCCACCGAGTACCTGGTGGTGCACCTC
  AAGGGCTCCCTGGTGAAGGACAGCCAGTATGAGATGGACAGCGAGTTCGAGGGGGAGTTG
  GCAGATGACCTGGCGGGCTTCTACCGCAGCGAGTACATGGAGGGCAATGTCAGAAAGGTG
  GTGGCCACTACACAGATGCAGGCTGCAGATGCCCGGAAGTCCTTCCCATGCTTCGATGAG
  CCGGCCATGAAGGCCGAGTTCAACATCACGCTTATCCACCCCAAGGACCTGACAGCCCTG
  TCCAACATGCTTCCCAAAGGTCCCAGCACCCCACTTCCAGAAGACCCCAACTGGAATGTC
  ACTGAGTTCCACACCACGCCCAAGATGTCCACGTACTTGCTGGCCTTCATTGTCAGTGAG
  TTCGACTACGTGGAGAAGCAGGCATCCAATGGTGTCTTGATCCGGATCTGGGCCCGGCCC
  AGTGCCATTGCGGTGGGCCACGGCGATTATGCCCTTAACGTGACAGGCCCCATCCTTAAC
  TTCTTTGCTGGTCATTATGACACACCCTACCCACTCCCAAAATCAGACCAGATTGGCCTG
  CCAGACTTCAACGCCGGCGCCATGGAGAACTGGGGACTGGTGACCTACCGGGAGAACTCC
  CTGCTGTTCGACCCCCTGTCCTCCTCCAGCAGCAACAAGGAGCGGGTGGTCACTGTGATT
  GCTCATGAGCTGGCCCACCAGTGGTTCGGGAACCTGGTGACCATAGAGTGGTGGAATGAC
  CTGTGGCTGAACGAGGGCTTCGCCTCCTACGTGGAGTACCTGGGTGCTGACTATGCGGAG
  CCCACCTGGAACTTGAAAGACCTCATGGTGCTGAATGATGTGTACCGCGTGATGGCAGTG
  GATGCACTGGCCTCCTCCCACCCGCTGTCCACACCCGCCTCGGAGATCAACACGCCGGCC
  CAGATCAGTGAGCTGTTTGACGCCATCTCCTACAGCAAGGGCGCCTCAGTCCTCAGGATG
  CTCTCCAGCTTCCTGTCCGAGGACGTATTCAAGCAGGGCCTGGCGTCCTACCTCCACACC
  TTTGCCTACCAGAACACCATCTACCTGAACCTGTGGGACCACCTGCAGGAGGCTGTGAAC
  AACCGGTCCATCCAACTCCCCACCACCGTGCGGGACATCATGAACCGCTGGACCCTGCAG
  ATGGGCTTCCCGGTCATCACGGTGGATACCAGCACGGGGACCCTTTCCCAGGAGCACTTC
  CTCCTTGACCCCGATTCCAATGTTACCCGCCCCTCAGAATTCAACTACGTGTGGATTGTG
  CCCATCACATCCATCAGAGATGGCAGACAGCAGCAGGACTACTGGCTGATAGATGTAAGA
  GCCCAGAACGATCTCTTCAGCACATCAGGCAATGAGTGGGTCCTGCTGAACCTCAATGTG
  ACGGGCTATTACCGGGTGAACTACGACGAAGAGAACTGGAGGAAGATTCAGACTCAGCTG
  CAGAGAGACCACTCGGCCATCCCTGTCATCAATCGGGCACAGATCATTAATGACGCCTTC
  AACCTGGCCAGTGCCCATAAGGTCCCTGTCACTCTGGCGCTGAACAACACCCTCTTCCTG
  ATTGAAGAGAGACAGTACATGCCCTGGGAGGCCGCCCTGAGCAGCCTGAGCTACTTCAAG
  CTCATGTTTGACCGCTCCGAGGTCTATGGCCCCATGAAGAACTACCTGAAGAAGCAGGTC
  ACACCCCTCTTCATTCACTTCAGAAATAATACCAACAACTGGAGGGAGATCCCAGAAAAC
  CTGATGGACCAGTACAGCGAGGTTAATGCCATCAGCACCGCCTGCTCCAACGGAGTTCCA
  GAGTGTGAGGAGATGGTCTCTGGCCTTTTCAAGCAGTGGATGGAGAACCCCAATAATAAC
  CCGATCCACCCCAACCTGCGGTCCACCGTCTACTGCAACGCTATCGCCCAGGGCGGGGAG
  GAGGAGTGGGACTTCGCCTGGGAGCAGTTCCGAAATGCCACACTGGTCAATGAGGCTGAC
  AAGCTCCGGGCAGCCCTGGCCTGCAGCAAAGAGTTGTGGATCCTGAACAGGTACCTGAGC
  TACACCCTGAACCCGGACTTAATCCGGAAGCAGGACGCCACCTCTACCATCATCAGCATT
  ACCAACAACGTCATTGGGCAAGGTCTGGTCTGGGACTTTGTCCAGAGCAACTGGAAGAAG
  CTTTTTAACGATTATGGTGGTGGCTCGTTCTCCTTCTCCAACCTCATCCAGGCAGTGACA
  CGACGATTCTCCACCGAGTATGAGCTGCAGCAGCTGGAGCAGTTCAAGAAGGACAACGAG
  GAAACAGGCTTCGGCTCAGGCACCCGGGCCCTGGAGCAAGCCCTGGAGAAGACGAAAGCC
  AACATCAAGTGGGTGAAGGAGAACAAGGAGGTGGTGCTCCAGTGGTTCACAGAAAACAGC
  AAATAGTCCCCAGCCCTTGAAGTCACCCGGCCCCGATGCAAGGTGCCCACATGTGTCCAT
  CCCAGCGGCTGGTGCAGGGCCTCCATTCCTGGAGCCCGAGGCACCAGTGTCCTCCCCTCA
  AGGACAAAGTCTCCAGCCCACGTTCTCTCTGCCTGTGAGCCAGTCTAGTTCCTGATGACC
  CAGGCTGCCTGAGCACCTCCCAGCCCCTGCCCCTCATGCCAACCCCGCCCTAGGCCTGGC
  ATGGCACCTGTCGCCCAGTGCCCTGGGGCTGATCTCAGGGAAGCCCAGCTCCAGGGCCAG
  ATGAGCAGAAGCTCTCGATGGACAATGAACGGCCTTGCTGGGGGCCGCCCTGTACCCTCT
  TTCACCTTTCCCTAAAGACCCTAAATCTGAGGAATCAACAGGGCAGCAGATCTGTATATT
  TTTTTCTAAGAGAAAATGTAAATAAAGGATTTCTAGATGAAAAAAAAAAAAAAAAAAAAC
  TCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001150 unedited
  GGGCGGGGGGNANAANACANTCNCCCCCCCGGGTTCAAAATTGTATACGATCATATAGGG
  CGGCCGCGAATCGGCACGAGCCAGTGCTGGAGGGGCAGGGACGGCGGCGGCGCAGCTCGG
  AACCCGCCAGGGTCCAGGGTCCAGGTTCCAGCGCCCGGCGGCCCAGCTCTCAAGCAGATC
  AATGCAATGCCACCTGGCCGCTTGCTTTGCCCACTGGGCCTACAGCCGGAAGCCTGCCCT
  TCAGCCCTCGGGCCTGATCCCAGGCCGCCTGCAGCCTGTAACCAGACACTGTTTGCTTCC
  AGCAGGCACCCCCCGAGCCCAGCTCCACACACCGTTCCTGGATCTCCTCTCCCCAGGCGG
  AGCGTGCCCCTGCCCAGTCCAGCGACCTTCGCCTGTTGGAGCCCTGGTTAATTTTTGCCC
  AGTCTGCCTGTTGTGGGGCTCCTCCCCTTTGGGGATATAAGCCCGGCCTGGGGCTGCTCC
  GTTCTCTGCCTGGCCTGAGGCTCCCTGAGCCGCCTCCCCACCATCACCATGGCCAAGGGC
  TTCTATATTTCCAAGTCCCTGGGCATCCTGGGGATCCTCCTGGGCGTGGCAGCCGTGTGC
  ACAATCATCGCACTGTCAGTGGTGTACTCCCAGGAGAAGAACAAGAACGCCAACAGCTCC
  CCCGTGGCCTCCACCACCCCGTCCGCCTCAGCCACCACCAACCCCGCCTCGGCCACCACC
  TTGGNACCAAAGTAAGCGTGGAATCGTTACCGCCTCCCCACACGCTGAAACCCGATTCCT
  ACCAGGTGACGCTGAGACCGTACCTCACCCCCCATGACAGGGGCCTGTACGTTNNTAAGG
  GCTNCAGCACCGTCCGTTTCACCTGCAGGGAGGCCACTGATGTCATCATCATCCCACAGC
  AGAAGCTCAACTACACCCTCANCAGGGGCACAGGGTGGTTCTGCGTGGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001150 unedited
  CGGGGCCNNNNNNNCCTTCNNNNANNAAGATTTGAACCCGGCCGCATTCTANGATCGGTT
  TTTTTTTTTTTTTTTTTTCATCAAAAAACCTTTATTTACATTTTCTCTTAGAAAAAAAAT
  ACAGATCTGCTGCCCTGTTGATTCCTCAAATTTAGGGTCTTTAGGGAAAGGTGAAAGAGG
  GTACAGGGCGGCCCCCAGCAAGGCCGTTCATTGTCCATCGAGAGCTTCTGCTCATCTGGC
  CCTGGAGCTGGGCTTCCCTGAGATCAGCCCCAGGGCACTGGGCGACAGGTGCCATGCCAG
  GCCTAGGGCGGGGTTGGCATGAGGGGCAGGGGCTGGGAGGTGCTCAGGCAGCCTGGGTCA
  TCAGGAACTAGACTGGCTCACAGGCAGAGAGAACGTGGGCTGGAGACTTTGTCCTTGAGG
  GGAGGACACTGGTGCCTCGGGCTCCAGGAATGGAGGCCCTGCACCAGCCGCTGGGATGGA
  CACATGTGGGCACCTTGCATCGGGGCCGGGTGACTTCAAGGGCTGGGGACTATTTGCTGT
  TTTCTGTGAACCACTGGAGCACCACCTCCTTGTTCTCCTTCACCCACTTGATGTTGGCTT
  TCGTCTTCTCCAGGGCTTGCTCCAGGGCCCGGGTGCCTGAGCCGAAGCCTGTTTCCTCGT
  TGNCCTTCTTGAACTGCTCCAGCTGCTGCAGCTCATACCTCGTGGAGAATCGTCGTGTCA
  CTGCCTGAATGAGGNTGGAGAAGGAGAACGAGCCACCACCATATCGTTAAAAGCTTCTTN
  CAGTTGCTCTGGNACAAGTCCNAGACCAGACTTGCCCATGACGNTGNTNGTAATGCTGAT
  GATGGGAAAGGTGGCGTCCTGCTTCCCGGATTAGTCCGGNTNCNGGGTGTAGCTCAGGTA
  CCCTGTCAGGATCCACCACTCTTTGCTGCAGCCAGGGCTTGCCNGGACTTGCCACCTCAT
  GAACACTGTGGCATTTCGGACTGCTCAAGGCGAATCCCACTCTCCTCCCGCCTGGGCGAT
  G
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001150
• Insert Size: 3750 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001150.1, NP_001141.1
• RefSeq Size: 3494 bp
• RefSeq ORF: 2904 bp
• Locus ID: 290
• UniProt ID: P15144
• Cytogenetics: 15q26.1
• Domains: Peptidase_M1
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease, Transmembrane
• Protein Pathways: Glutathione metabolism, Hematopoietic cell lineage, Metabolic pathways, Renin-angiotensin system
• Gene Summary: Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. This membrane-bound zinc metalloprotease is known to serve as a receptor for the HCoV-229E alphacoronavirus as well as other non-human coronaviruses. This gene has also been shown to promote angiogenesis, tumor growth, and metastasis and defects in this gene are associated with various types of leukemia and lymphoma. [provided by RefSeq, Apr 2020]