AMFR (NM_001144) Human Untagged Clone

CAT#: SC119417

AMFR (untagged)-Human autocrine motility factor receptor (AMFR)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMFR
• Synonyms: GP78; RNF45
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119417 sequence for NM_001144 edited (data generated by NextGen Sequencing)
  ATGCCGCTGCTCTTCCTCGAGCGCTTCCCCTGGCCCAGCCTCCGCACCTACACGGGCCTC
  AGCGGCCTGGCCCTGCTGGGCACCATCATCAGCGCCTACCGCGCGCTCAGCCAGCCCGAG
  GCCGGCCCCGGCGAGCCGGACCAGCTAACGGCCTCGCTGCAGCCTGAGCCGCCGGCGCCC
  GCCCGGCCGAGCGCCGGGGGACCCCGGGCCCGCGATGTGGCCCAGTACCTGCTCTCAGAC
  AGCCTCTTCGTGTGGGTTCTAGTAAATACCGCTTGCTGTGTTTTGATGTTGGTGGCTAAG
  CTCATCCAGTGTATTGTGTTTGGCCCTCTTCGAGTGAGTGAGAGACAGCATCTCAAAGAC
  AAATTTTGGAATTTTATTTTCTACAAGTTCATTTTCATCTTTGGTGTGCTGAATGTCCAG
  ACAGTGGAAGAGGTGGTCATGTGGTGCCTCTGGTTTGCCGGACTTGTCTTTCTGCACCTG
  ATGGTTCAGCTCTGCAAGGATCGATTTGAATATCTTTCCTTCTCGCCCACCACGCCGATG
  AGCAGCCACGGTCGAGTCCTGTCCCTGTTGGTTGCCATGCTGCTTTCCTGCTGTGGACTG
  GCGGCCGTCTGCTCCATCACCGGCTACACCCACGGAATGCACACCTTGGCTTTCATGGCT
  GCAGAGTCTCTTCTTGTGACAGTGAGGACTGCTCATGTGATTTTACGATACGTAATTCAC
  CTCTGGGACCTCAACCACGAAGGGACGTGGGAAGGAAAGGGGACGTATGTCTATTACACA
  GACTTTGTCATGGAGCTCACTCTCCTGTCCCTGGACCTCATGCACCATATTCACATGTTG
  TTATTTGGCAACATCTGGTTATCCATGGCCAGCCTGGTCATCTTTATGCAGCTGCGTTAC
  CTGTTTCATGAGGTGCAACGTCGAATTCGTCGGCACAAGAACTATCTACGTGTGGTTGGA
  AACATGGAGGCCAGGTTTGCAGTTGCAACTCCAGAGGAGCTGGCTGTCAACAATGACGAC
  TGTGCCATCTGTTGGGACTCCATGCAGGCTGCGCGGAAACTGCCCTGTGGACATCTTTTC
  CACAACTCCTGTCTTCGTTCCTGGCTAGAACAAGACACCTCCTGTCCAACATGCAGAATG
  TCTCTTAATATTGCCGACAATAATCGTGTCAGGGAAGAACATCAAGGAGAGAACTTGGAT
  GAGAATTTGGTTCCTGTAGCAGCAGCCGAAGGGAGACCTCGCTTAAACCAACACAATCAC
  TTCTTCCATTTCGATGGGTCTCGGATTGCGAGCTGGCTGCCGAGTTTTTCGGTTGAAGTG
  ATGCACACCACCAACATTCTTGGCATTACGCAGGCCAGCAACTCCCAGCTCAATGCAATG
  GCTCATCAGATTCAAGAGATGTTTCCCCAGGTTCCATACCATCTGGTACTGCAGGACCTC
  CAGCTGACACGCTCAGTTGAAATAACAACAGACAATATTTTAGAAGGACGGATTCAAGTA
  CCTTTTCCTACACAGCGGTCAGATAGCATCAGACCTGCATTGAACAGTCCTGTGGAAAGG
  CCAAGCAGTGACCAGGAAGAGGGAGAAACTTCTGCTCAGACCGAGCGTGTGCCACTGGAC
  CTCAGTCCTCGCCTGGAGGAGACGCTGGACTTCGGCGAGGTGGAAGTGGAGCCCAGTGAG
  GTGGAAGACTTCGAGGCTCGTGGGAGCCGCTTCTCCAAGTCTGCTGATGAGAGACAGCGC
  ATGCTGGTGCAGCGTAAGGACGAACTCCTCCAGCAAGCTCGCAAACGTTTCTTGAACAAA
  AGTTCTGAAGATGATGCGGCCTCAGAGAGCTTCCTCCCCTCGGAAGGTGCGTCCTCTGAC
  CCCGTGACCCTGCGTCGAAGGATGCTGGCTGCCGCCGCGGAACGGAGGCTTCAGAAGCAG
  CAGACCTCCTAG
  
  Clone variation with respect to NM_001144.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001144 unedited
  GTGCAAATTTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACCAGCCGCCAT
  GCCGCTGCTCTTCCTCGAGCGCTTCCCCTGGCCCAGCCTCCGCACCTACACGGGCCTCAG
  CGGCCTGGCCCTGCTGGGCACCATCATCAGCGCCTACCGCGCGCTCAGCCAGCCCGAGGC
  CGGCCCCGGCGAGCCGGACCAGCTAACGGCCTCGCTGCAGCCTGAGCCGCCGGCGCCCGC
  CCGGCCGAGCGCCGGGGGACCCCGGGCCCGCGATGTGGCCCAGTACCTGCTCTCAGACAG
  CCTCTTCGTGTGGGTTCTAGTAAATACCGCTTGCTGTGTTTTGATGTTGGTGGCTAAGCT
  CATCCAGTGTATTGTGTTTGGCCCTCTTCGAGTGAGTGAGAGACAGCATCTCAAAGACAA
  ATTTTGGAATTTTATTTTCTACAAGTTCATTTTCATCTTTGGTGTGCTGAATGTCCAGAC
  AGTGGAAGAGGTGGTCATGTGGTGCCTCTGGTTTGCCGGACTTGTCTTTCTGCACCTGAT
  GGTTCAGCTCTGCAAGGATCGATTTGAATATCTTTCCTTCTCGCCCACCACGCCGATGAG
  CAGCCACGGTCGAGTCCTGTCCCTGTTGGTTGCCATGCTGCTTTCCTGCTGTGGACTGGC
  GGCCGTCTGCTCCATCACCGGCTACACCCACGGAATGCACACCTTGGCTTTCATGGCTGC
  AGAGTCTCTTCTTGTGACAGTGAGGACTGCTCATGTGATTNTACGATACGTAATTCACCT
  CTGGGACCTCAACCACGAAGGGNACGTGGGAAGAAAGGGGACGTATGTCTATACACAGAC
  TTTGTCATGGAGCTCACTCTCCTGTCCCTGGGACTCATGCCCCATATTCACATGGTTGTN
  ATTTGCCACATCTTGGTTATCCATGGCCAGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001144
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001144.3, NP_001135.3
• RefSeq Size: 3453 bp
• RefSeq ORF: 1932 bp
• Locus ID: 267
• UniProt ID: Q9UKV5
• Cytogenetics: 16q13
• Domains: RING, CUE
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012]
  Transcript Variant: This variant (1) is the longer transcript and encodes the longer isoform (a).