CIRBP (NM_001280) Human Untagged Clone

CAT#: SC119359

CIRBP (untagged)-Human cold inducible RNA binding protein (CIRBP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CIRBP
• Synonyms: CIRP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001280 edited
  ATGGCATCAGATGAAGGCAAACTTTTTGTTGGAGGGCTGAGTTTTGACACCAATGAGCAG
  TCGCTGGAGCAGGTCTTCTCAAAGTACGGACAGATCTCTGAAGTGGTGGTTGTGAAAGAC
  AGGGAGACCCAGAGATCTCGGGGATTTGGGTTTGTCACCTTTGAGAACATTGACGACGCT
  AAGGATGCCATGATGGCCATGAATGGGAAGTCTGTAGATGGACGGCAGATCCGAGTAGAC
  CAGGCAGGCAAGTCGTCAGACAACCGATCCCGTGGGTACCGTGGTGGCTCTGCCGGGGGC
  CGGGGCTTCTTCCGTGGGGGCCGAGGACGGGGCCGTGGGTTCTCTAGAGGAGGAGGGGAC
  CGAGGCTATGGGGGGAACCGGTTCGAGTCCAGGAGTGGGGGCTACGGAGGCTCCAGAGAC
  TACTATAGCAGCCGGAGTCAGAGTGGTGGCTACAGTGACCGGAGCTCGGGCGGGTCCTAC
  AGAGACAGTTATGACAGTTACGCTACACACAACGAGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001280 unedited
  TGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTCGGGTCGTTGTGGTGCG
  CTGTCTTCCCGCTTGCGTCAGGGACCTGCCCGACTCAGTGGCCGCCATGGCATCAGATGA
  AGGCAAACTTTTTGTTGGAGGGCTGAGTTTTGACACCAATGAGCAGTCGCTGGAGCAGGT
  CTTCTCAAAGTACGGACAGATCTCTGAAGTGGTGGTTGTGAAAGACAGGGAGACCCAGAG
  ATCTCGGGGATTTGGGTTTGTCACCTTTGAGAACATTGACGACGCTTAGGATGCCATGAT
  GGCCATGAATGGGAAGTCTGTAGATGGACGGCAGATCCGAGTAGACCAGGCAGGCAAGTC
  GTCAGACAACCGATCCCGTGGGTACCGTGGTGGCTCTGCCGGGGGCCGGGGCTTCTTCCG
  TGGGGGCCGAGGACGGGGCCGTGGGTTCTCTAGAGGAGGAGGGGACCGAGGCTATGGGGG
  GAACCGGTTCGAGTCCAGGAGTGGGGGCTACGGAGGCTCCAGAGACTACTATAGCAGCCG
  GAGTCAGAGTGGTGGCTACAGTGACCGGAGCTCGGGCGGGTCCTACAGAGACAGTTATGA
  CAGTTACGCTACACACAACGAGTAAAAACCCTTCCTGCTCAAGATCGTCCTTCCAATGGG
  CTGTGTGTTTAAAGATTGTGGGAGCTTCGCTGAACGTTAATGTGTAGTAAATGCACCTCC
  TTGTATTCCCACTTTCGAGTCTTTTTTCGGGTTCTGATCTTGTCAAACCCAGCCTGACCG
  CTTCTGACGCCCGGGATGGCCTCNGTACTAGACTTTCTTTTTAAAGGAAGGGGTTGGTTT
  TTTTTTGGGGGGGTTTTCAAACATTTTGAAAAGCATTTACTTTTTGACCCAGCCATGAGT
  TAAAAGGGGGGGGGGGGGGGGGGGGGNNNNNNNNGGGNNNNNNAAAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001280 unedited
  GCGGCACGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTCATTTAACACGNGTCTTTT
  AATCGGCTTCGTAAAGCCAAAAAAAACTGCGTACAAGAATTCCTCAGCCACTGCAAATAC
  AACATCGATGGAGTATGCGTACGTCTTCCAGTAAAAAAGGACAATATAAATAAACATCTT
  CAAGAACCTCTCAGGGACGTCTGCACGTCTACTGTGGGGCGCACAGCACAGATGGACGCA
  GGGGCTGGGGCTCCAGGGCAGTTGCTGCCACACCCGCCGGGGTCCTACCACACTACACTC
  AGAAACGTCACACGGAGTTGGAAAAAAGCCACAGCTTGCTCAGCTTCCGACAAAGAGCGG
  ATTCACAGTCCGTTCGATCTCAGAGAAGTGAGTGGGGCACCCACTTCATGGGGCCAACCC
  CACTAAAAAAAAAAAGGCAACGCAACCAAAAACTAAAACAAAAACCAAAAACCCACACAA
  CCCCCGATTTTTTTGAAAACTCATGGCTCGTGGTCAAAAAAGTAAATGCTTTTCAAAATG
  TTTTGAAAACCCTCAAAAAAAAACAGCACTTCCTTAAAAAGAAAAGTCTAGTAACGAGGC
  CATCCCGGCGTCAGAAGCGGTCAGGCTGGGTTTGACAAGATCAGAACCGAAATGACTACG
  AAAGTGGGAATACAAGGAGGTGCATTTACTACACATTAACGTTCAGCGAAGCTCCCACAA
  TCTTTAAACACACAGCCATTGGAAGGACGATCTTGAGCAGGAAGGGTTTTTACTCGTTGT
  GTGTAGCGTAACTGTCATAACTGTCTCTGTNAGACCCGNCCCGAGCTCGGTCACTGTAGC
  CACCACTCTGACTNNCGCTGCTATAGTAGTCTCTGGAGCCTNCGTAGCCCCACTCCTGGA
  CTCGNACCGGTNTCCCNCATAGCCTCGGTCCCCTCCTCTCTAGAAAACCCACGCCCGTNN
  CTCGCCCCAGNAAGAGCCCGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001280
• Insert Size: 1260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001280.1, NP_001271.1
• RefSeq Size: 1322 bp
• RefSeq ORF: 519 bp
• Locus ID: 1153
• UniProt ID: Q14011
• Cytogenetics: 19p13.3
• Domains: RRM, RRM_1
• Gene Summary: Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3' UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) uses alternate splice sites at the 3' end, resulting in a novel 3' coding region and 3' UTR, compared to variant 5. It encodes isoform 1, which has a shorter and distinct C-terminus, compared to isoform 3.