Chromogranin A (CHGA) (NM_001275) Human Untagged Clone

SKU SC119356

CHGA (untagged)-Human chromogranin A (parathyroid secretory protein 1) (CHGA)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Chromogranin A
• Synonyms: CGA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119356 sequence for NM_001275 edited (data generated by NextGen Sequencing); ATGCGCTCCGCCGCTGTCCTGGCTCTTCTGCTCTGCGCCGGGCAAGTCACTGCGCTCCCT; GTGAACAGCCCTATGAATAAAGGGGATACCGAGGTGATGAAATGCATCGTTGAGGTCATC; TCCGACACACTTTCCAAGCCCAGCCCCATGCCTGTCAGCCAGGAATGTTTTGAGACACTC; CGAGGAGATGAACGGATCCTTTCCATTCTGAGACATCAGAATTTACTGAAGGAGCTCCAA; GACCTCGCTCTCCAAGGCGCCAAGGAGAGGGCACATCAGCAGAAGAAACACAGCGGTTTT; GAAGATGAACTCTCAGAGGTTCTTGAGAACCAGAGCAGCCAGGCCGAGCTGAAAGAGGCG; GTGGAAGAGCCATCATCCAAGGATGTTATGGAGAAAAGAGAGGATTCCAAGGAGGCAGAG; AAAAGTGGTGAAGCCACAGACGGAGCCAGGCCCCAGGCCCTCCCGGAGCCCATGCAGGAG; TCCAAGGCTGAGGGGAACAATCAGGCCCCTGGGGAGGAAGAGGAGGAGGAGGAGGAGGCC; ACCAACACCCACCCTCCAGCCAGCCTCCCCAGCCAGAAATACCCAGGCCCACAGGCCGAG; GGGGACAGTGAGGGCCTCTCTCAGGGTCTGGTGGACAGAGAGAAGGGCCTGAGTGCAGAG; CCAGGGTGGCAGGCAAAGAGAGAAGAGGAGGAGGAGGAGGAGGAGGAGGCTGAGGCTGGA; GAGGAGGCTGTCCCCGAGGAAGAAGGCCCCACTGTAGTGCTGAACCCCCACCCGAGCCTT; GGCTACAAGGACATCCGGAAAGGCGAGAGTCGGTCGGAGGCTCTGGCTGTGGATGGAGCT; GGGAAGCCTGGGGCTGAGGAGGCTCAGGACCCCGAAGGGAAGGGAGAACAGGAGCACTCC; CAGCAGAAAGAGGAGGAGGAGGAGATGGCAGTGGTCCCGCAAGGCCTCTTCCGGGGTGGG; AAGAGCGGAGAGCTGGAGCAGGAGGAGGAGCGGCTCTCCAAGGAGTGGGAGGACTCCAAA; CGCTGGAGCAAGATGGACCAGCTGGCCAAGGAGCTGACGGCTGAGAAGCGGCTGGAGGGG; CAGGAGGAGGAGGAGGACAACCGGGACAGTTCCATGAAGCTCTCCTTCCGGGCCCGGGCC; TACGGCTTCAGGGGCCCTGGGCCGCAGCTGCGACGAGGCTGGAGGCCATCCTCCCGGGAG; GACAGCCTTGAGGCGGGCCTGCCCCTCCAGGTCCGAGGCTACCCCGAGGAGAAGAAAGAG; GAGGAGGGCAGCGCAAACCGCAGACCAGAGGACCAGGAGCTGGAGAGCCTGTCGGCCATT; GAGGCAGAGCTGGAGAAAGTGGCCCACCAGCTGCAGGCACTACGGCGGGGCTGA; ; Clone variation with respect to NM_001275.3; 792 g=>c;1323 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001275 unedited; GATTTATGTATACGACTTACTATAGTGGCGGCCGCGATTCGGCACGAGGTGCAGTGCTCG; AGCCCCGTGCAGGGGAGCTTGCGGGAGGATCGACCGACAGACGGACGCACGCCGAGGCAC; TGCGCCCCCAGCCCCGCGCCGGTGCCACCGCAGCCCGACCCCGGCCGCCAGTCCAGCCGC; CCCTCGCCCGGTGCCTAGGTGCCCGGCCCCACACCGCCAGCTGCTCGGCGCCCGGGTCCG; CCATGCGCTCCGCCGCTGTCCTGGCTCTTCTGCTCTGCGCCGGGCAAGTCACTGCGCTCC; CTGTGAACAGCCCTATGAATAAAGGGGATACCGAGGTGATGAAATGCATCGTTGAGGTCA; TCTCCGACACACTTTCCAAGCCCAGCCCCATGCCTGTCAGCCAGGAATGTTTTGAGACAC; TCCGAGGAGATGAACGGATCCTTTCCATTCTGAGACATCAGAATTTACTGAAGGAGCTCC; AAGACCTCGCTCTCCAAGGCGCCAAGGAGAGGGCACATCAGCAGAAGAAACACAGCGGTT; TTGAAGATGAACTCTCAGAGGTTCTTGAGAACCAGAGCAGCCAGGCCGAGCTGAAAGAGG; CGGTGGAAGAGCCATCATCCAAGGATGTTATGGAGAAAAGAGAGGATTCCAAGGAGGCAG; AGAAAAGTGGTGAAGCCACAGACGGAGCCCGGCCCAAGCCCTTCCGGAGCCCATGCAGGA; GTCCAAGCCTGAGGGGAACAATCANGCCCCTGGGGAGGAACAAGAGTGAGAGGAGGAGGC; CACCCACACCACCCTTCAGNCAGTCTTCCCAGCCAGATTACCCAGGCCCACAGGCCGAGA; GGACAGGGAGGGCCNTTCTAAGGTCTGGTGGACAGAGAAAGGCCCTGATGCAAAGCCAGG; TGGCAGCAAGAAGAAAGGGGGAGAAGAGTAGTAGGGCTCTAGCCTGAAGGAGGTGTCCCT; AGACAAGGCCCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001275 unedited; GCGTTTCGGGAGACCCANACAGCTTTATTTTTCTTTAGNTAAAGTTCATACAGAATATGT; TACAGTCAGGAGTTCTCAGCTTTCACCAAAGGTCAGAAGTTATTGCAGTTGTGCCCAGTG; GATAGGATGGAGGAAGGGAAACTTCTAGAAAGCTGCCTGTGTTCAGAGAAGGAATCGTCA; ATGTTTAAAGTTGTGGGGCTGCCCTGCAAAGGATGTTCCAGGGCAGGTAGGAGTCAGGAG; TAGGAGACAAGGCGCGTAGGGGGCGATAGGGTGGCCTGGGCTTGGGCAGGCTGGAGGCTG; CCTCCCTACCGGAAGCAGCGTCCGGGCCATCTGGCCAGGACCTGCCAAGGGGACAGCAGA; GCCAGGGCCACAGGGTGCCCTGGGGCCAGCCCTGCCAGCCGGTGTCTCAGCCCCGCCGTA; GTGCCTGCAGCTGGTGGGCCACTTTCTCCAGCTCTGCCTCAATGGCCGACAGGCTCTCCA; GCTCCTGGTCCTCTGGTCTGCGGTTTGCGCTGCCCTCCTCCTCTTTCTTCTCCTCGGGGT; AGCCTCGGACCTGGAGGGGCAGGCCCGCCTCAAGGCTGTCCTCCCGGGAGGATGGCCTCC; AGCCTCGTCGCAGCTGCGGCCCAGGGCCCCTGAAGCCGTAGGCCCGGGCCCGGAAGGAGA; GCTTCATGGAACTGTCCCGGTTGTCCTCCTCCTTCTCCTGCCCCTCCAGCCGCTTCTCAG; CCGTCAGCTCCTTGGCCAGCTGGTCCATCTTGCTCCAGCGTTTGGAGTCCTCCCACTCCT; TGGAGAGCCGNTCCTCCTNCTGCTCCAGCTCTCCGCTCTTTCCACCCCCGNAGAAGCCTT; GCGGGACCACTGNCATTTNCTNCTCCTNCTCTTTCTGCTGGGAGTGCTCCTGTNCTCCCT; TCCTTTCGGGTCTGAGCCTTCTCAGCCCAGCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_001275
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001275.2, NP_001266.1
• RefSeq Size: 1994 bp
• RefSeq ORF: 1374 bp
• Locus ID: 1113
• UniProt ID: P10645
• Cytogenetics: 14q32.12
• Domains: Granin
• Protein Families: Druggable Genome, Secreted Protein
• Summary: The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).