CDK9 (NM_001261) Human Untagged Clone

SKU SC119344

CDK9 (untagged)-Human cyclin-dependent kinase 9 (CDK9)

Product Data

• Type: Human Untagged Clone
• Target Symbol: CDK9
• Synonyms: C-2k; CDC2L4; CTK1; PITALRE; TAK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001261 edited; ATGGCAAAGCAGTACGACTCGGTGGAGTGCCCTTTTTGTGATGAAGTTTCCAAATACGAG; AAGCTCGCCAAGATCGGCCAAGGCACCTTCGGGGAGGTGTTCAAGGCCAGGCACCGCAAG; ACCGGCCAGAAGGTGGCTCTGAAGAAGGTGCTGATGGAAAACGAGAAGGAGGGGTTCCCC; ATTACAGCCTTGCGGGAGATCAAGATCCTTCAGCTTCTAAAACACGAGAATGTGGTCAAC; TTGATTGAGATTTGTCGAACCAAAGCTTCCCCCTATAACCGCTGCAAGGGTAGTATATAC; CTGGTGTTCGACTTCTGCGAGCATGACCTTGCTGGGCTGTTGAGCAATGTTTTGGTCAAG; TTCACGCTGTCTGAGATCAAGAGGGTGATGCAGATGCTGCTTAACGGCCTCTACTACATC; CACAGAAACAAGATCCTGCATAGGGACATGAAGGCTGCTAATGTGCTTATCACTCGTGAT; GGGGTCCTGAAGCTGGCAGACTTTGGGCTGGCCCGGGCCTTCAGCCTGGCCAAGAACAGC; CAGCCCAACCGCTACACCAACCGTGTGGTGACACTCTGGTACCGGCCCCCGGAGCTGTTG; CTCGGGGAGCGGGACTACGGCCCCCCCATTGACCTGTGGGGTGCTGGGTGCATCATGGCA; GAGATGTGGACCCGCAGCCCCATCATGCAGGGCAACACGGAGCAGCACCAACTCGCCCTC; ATCAGTCAGCTCTGCGGCTCCATCACCCCTGAGGTGTGGCCAAACGTGGACAACTATGAG; CTGTACGAAAAGCTGGAGCTGGTCAAGGGCCAGAAGCGGAAGGTGAAGGACAGGCTGAAG; GCCTATGTGCGTGACCCATACGCACTGGACCTCATCGACAAGCTGCTGGTGCTGGACCCT; GCCCAGCGCATCGACAGCGATGACGCCCTCAACCACGACTTCTTCTGGTCCGACCCCATG; CCCTCCGACCTCAAGGGCATGCTCTCCACCCACCTGACGTCCATGTTCGAGTACTTGGCA; CCACCGCGCCGGAAGGGCAGCCAGATCACCCAGCAGTCCACCAACCAGAGTCGCAATCCC; GCCACCACCAACCAGACGGAGTTTGAGCGCGTCTTCTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001261 unedited; TGTAATACGACTCACTATAGGGCGGCCGCNGAATCGGCACGAGGCTCCGGCGCCCCGGCT; CCCCGCGCCCCCGATCGGGGCCGCCGCTAGTAGTGGCGGCGGCGGAGGCGGGGGCAGCGG; CGGCGGCGGCGGAGGCGCCTCTGCAGCTCCGGCTCCCCCTGGCCTCTCGGGAACTACAAG; TCCCAGGGGGCCTGGCGGTGGGCGGCGGGCGGAAGAGGCGGGGTCGGCGCCGCGAGGCCG; GAAGTGGCCGTGGAGGCGGAAGTGGCGCGGCCGCGGAGGGGCCTGGAGTGCGGCGGCGGC; GGGACCCGGAGCAGGAGCGGCGGCAGCAGCGACTGGGGGCGGCGGCGGCGCGTTGGAGGC; GGCCATGGCAAAGCAGTACGACTCGGTGGAGTGCCCTTTTTGTGATGAAGTTTCCAAATA; CGAGAAGCTCGCCAAGATCGGCCAAGGCACCTTCGGGGAGGTGTTCAAGGCCAGGCACCG; CAAGACCGGCCAGAAGGTGGCTCTGAAGAAGGTGCTGATGGAAAACGAGAAGGAGGGGTT; CCCCATTACAGCCTTGCGGGAGATCAAGATCCTTCAGCTTCTAAAACACGAGAATGTGGT; CAACTTGATTGAGATTTGTCGAACCAAAGCTTCCCCCTATAACCGCTGCAAGGGTAGTAT; ATACCCTGGTGTTCGACTTCTGCGAGCATGACCTTGCTGGGCTGTTGAGCAATGTTNTGG; TCAAGTTCACGCTGTCTGAGATCAAGAAGGTGATGCAGATGCTGCTTAACGGCCTCTACT; ACATCCACAGAAACAAGATCCTGCATAGGGACATGAAGGCTGCTAATGTGCTTACACTCG; TGGATGGGGTCCTGAGCTGGCGACTTTGGGCTGGCCCGGNCCTTCACCTGGCAAGAACAG; CAGCCCACCGTACACACCGGTGTGTGACCTCTG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001261 unedited; TACTTGGGGAAAAAATTTACAAAAATAAAATTNCAACTTTTTTAATTCTAACGGAACCAA; ACTGTGCCCAGGAATGTCTCCAAACAATGGAACCAAAAGAAACACAGTAAAATTCTCCTG; TTGAAGAAACCAAGAGTTTGTGCCAGCCCCAGTGTTTCTGAGGGTTAGGCCAAAAACAGC; CCATTCCTGAGGCGAGCCCTGTCCCCACGCAGGGTTCTCAGCCCGAGAATAGGATTGTGG; GTGGGTGAGGGACCTGTCCCCTCTTCCACCACGCCGGGAGCTCTTAGAAAAAGTCACTGG; TGGGCGAGCCCATCCTCCTACCCCATGGAAACCCTCTGGGAACCATCCAGAAAACCAGCA; AGGACAAGACAGCTCCAGTGCCCTCCTGGTCTCTCAGCCAATGCTCCACTCCAGTCCACT; CAGCGGGCTGCTCCCAGAGCCCAGGGTGGGGATTAAATAAAATATGCATGAGAGATATAA; ACTCAAATGCCCTGTCTCCACGATGCAAGTCACATAGCAGAAGAAAAAAAACACAAGAGC; CCTAGTGGCAAGCGCCGGCCCTCAGAAGACGCGCTCAAACTCCGTCTGGTTGGTGGTGGC; GGGATTGCGACTCTGGTTGGTGGACTGCTGGGTGATCTGGCTGCCCTTCCGGCGCGGTGG; TGCCAAGTACTCCAACATGGACGTCAGGTGGGGTGGAGAGCATGCCCTTTGAGTCCGAGG; GCATGGGGTCGGACCAAANAAGTCGTGGTTGAAGGCGTCATCGCTGTCCATGCGCTGGGC; CAGGTCCAACACCACCACCTTTGTCATGAGGGCCAATGCCTATGGGTCACGCCATAAGCC; CTTAAACTGGCCTTTACCTTCCCCTTTTGGCCCTTGACCAGCTCAGCTTTTTCCTCCGCT; CATAAGTGTTCACCTTGGGCCACACCTCCGGGGTGAAGGGAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_001261
• Insert Size: 1910 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001261.2, NP_001252.1
• RefSeq Size: 1791 bp
• RefSeq ORF: 1119 bp
• Locus ID: 1025
• UniProt ID: P50750
• Cytogenetics: 9q34.11
• Domains: pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Summary: The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]