CDC6 (NM_001254) Human Untagged Clone
Product Data | |
Type | Human Untagged Clone |
---|---|
Target Symbol | CDC6 |
Synonyms | CDC18L; HsCDC6; HsCDC18; MGORS5 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
Fully Sequenced ORF
>OriGene ORF within SC119336 sequence for NM_001254 edited (data generated by NextGen Sequencing)
ATGCCTCAAACCCGATCCCAGGCACAGGCTACAATCAGTTTTCCAAAAAGGAAGCTGTCT CGGGCATTGAACAAAGCTAAAAACTCCAGTGATGCCAAACTAGAACCAACAAATGTCCAA ACCGTAACCTGTTCTCCTCGTGTAAAAGCCCTGCCTCTCAGCCCCAGGAAACGTCTGGGC GATGACAACCTATGCAACACTCCCCATTTACCTCCTTGTTCTCCACCAAAGCAAGGCAAG AAAGAGAATGGTCCCCCTCACTCACATACACTTAAGGGACGAAGATTGGTATTTGACAAT CAGCTGACAATTAAGTCTCCTAGCAAAAGAGAACTAGCCAAAGTTCACCAAAACAAAATA CTTTCTTCAGTTAGAAAAAGTCAAGAGATCACAACAAATTCTGAGCAGAGATGTCCACTG AAGAAAGAATCTGCATGTGTGAGACTATTCAAGCAAGAAGGCACTTGCTACCAGCAAGCA AAGCTGGTCCTGAACACAGCTGTCCCAGATCGGCTGCCTGCCAGGGAAAGGGAGATGGAT GTCATCAGGAATTTCTTGAGGGAACACATCTGTGGGAAAAAAGCTGGAAGCCTTTACCTT TCTGGTGCTCCTGGAACTGGAAAAACTGCCTGCTTAAGCCGGATTCTGCAAGACCTCAAG AAGGAACTGAAAGGCTTTAAAACTATCATGCTGAATTGCATGTCCTTGAGGACTGCCCAG GCTGTATTCCCAGCTATTGCTCAGGAGATTTGTCAGGAAGAGGTATCCAGGCCAGCTGGG AAGGACATGATGAGGAAATTGGAAAAACATATGACTGCAGAGAAGGGCCCCATGATTGTG TTGGTATTGGACGAGATGGATCAACTGGACAGCAAAGGCCAGGATGTATTGTACACGCTA TTTGAATGGCCATGGCTAAGCAATTCTCACTTGGTGCTGATTGGTATTGCTAATACCCTG GATCTCACAGATAGAATTCTACCTAGGCTTCAAGCTAGAGAAAAATGTAAGCCACAGCTG TTGAACTTCCCACCTTATACCAGAAATCAGATAGTCACTATTTTGCAAGATCGACTTAAT CAGGTATCTAGAGATCAGGTTCTGGACAATGCTGCAGTTCAATTCTGTGCCCGCAAAGTC TCTGCTGTTTCAGGAGATGTTCGCAAAGCACTGGATGTTTGCAGGAGAGCTATTGAAATT GTAGAGTCAGATGTCAAAAGCCAGACTATTCTCAAACCACTGTCTGAATGTAAATCACCT TCTGAGCCTCTGATTCCCAAGAGGGTTGGTCTTATTCACATATCCCAAGTCATCTCAGAA GTTGATGGTAACAGGATGACCTTGAGCCAAGAAGGAGCACAAGATTCCTTCCCTCTTCAG CAGAAGATCTTGGTTTGCTCTTTGATGCTCTTGATCAGGCAGTTGAAAATCAAAGAGGTC ACTCTGGGGAAGTTATATGAAGCCTACAGTAAAGTCTGTCGCAAACAGCAGGTGGCGGCT GTGGACCAGTCAGAGTGTTTGTCACTTTCAGGGCTCTTGGAAGCCAGGGGCATTTTAGGA TTAAAGAGAAACAAGGAAACCCGTTTGACAAAGGTGTTTTTCAAGATTGAAGAGAAAGAA ATAGAACATGCTCTGAAAGATAAAGCTTTAATTGGAAATATCTTAGCTACTGGATTGCCT TAA Clone variation with respect to NM_001254.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_001254 unedited
NCCGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCTTGT GGTGGTGAGTCCGAGAGGCTGCGTGTGAGAGACGTGAGAAGGATCCTGCACTGAGGAGGT GGAAAGAAGAGGATTGCTCGAGGAGGCCTGGGGTCTGTGAGGCAGCGGAGCTGGGTGAAG GCTGCGGGTTCCGGCGAGGCCTGAGCTGTGCTGTCGTCATGCCTCAAACCCGATCCCAGG CACAGGCTACAATCAGTTTTCCAAAAAGGAAGCTGTCTCGGGCATTGAACAAAGCTAAAA ACTCCAGTGATGCCAAACTAGAACCAACAAATGTCCAAACCGTAACCTGTTCTCCTCGTG TAAAAGCCCTGCCTCTCAGCCCCAGGAAACGTCTGGGCGATGACAACCTATGCAACACTC CCCATTTACCTCCTTGTTCTCCACCAAAGCAAGGCAAGAAAGAGAATGGTCCCCCTCACT CACATACACTTAAGGGACGAAGATTGGTATTTGACAATCAGCTGACAATTAAGTCTCCTA GCAAAAGAGAACTAGCCAAAGTTCACCAAAACAAAATACTTTCTTCAGTTAGAAAAAGTC AAGAGATCACAACAAATTCTGAGCAGAGATGTCCACTGAAGAAAGAATCTGCATGTGTGA GACTATTCAAGCAAGAAGGCACTTGCTACCAGCAAGCAAAGCTGGTCCTGAACACAGCTG TCCCAGATCGGCTGCCTGCNCAGGAAAGGGAGATGGATGTCATCAGGAATTTCTTGAGGG AACACATCTGTGGGAAAAAGCTGGAAGCCTTTACCTTTCTGGTGCTCCTGGNACTGGAAA ACTGCCTGCTTAAGCCGNATTCTGNCAGACCTCAAGAAGGAACTGAAAGGCTTTAAACTA TCTGCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_001254 unedited
ACCGCGGGCCGCAATTTANAGTCGAGTTTTTTTTTTTTTTTTTTTGCAACTTGAAAATAA ATATATTCACAATCTGTAGAGTTATAGTCTCCTTATGACCCCAACGCCCTTGGGAGTGCC CAAGAAACTCAACGAGTCTAAAATGCCACAATGTTGTAGTATCTGCACCATGAACATAGT ATACTATACAAAGAAATAAATTCTTTAACCAGTGTCCTCCAAATCTCCATGTGGACTTGC TTGCATTTTTCTGGATCATTTTCATTCTTCTTACCCTAAACTTAAAGTTCTTAACAAAAT ACCTTAATGGCTGAGCATGGTGGCTCACGCCTATAATCCCAGCACTTAGGGAGGCCAAGG TGGGCAGATCACTTGAGGGTCAGGAGTTCAAGACCAGCCTGGCCAACATGGTAAAACCCT GTCTCTACTAAAAATTAAAAAATTACCTGGGCGCGGTGGTGGGCACCTGTAATCCCAGCT ACTCGGGAGGCTGAGGCAAGAGAATCACTTCAACCTGGGAAGCGGGCGCTGTACTGAGCA AAGAACGCGCCATTGCACTCCAGCCTGTGCAACAGGGTGAGACGCGCCTCAAAAACAACA CCACCAAACACACACAACAAAAAAATCCCCACTCATGCTTGAGCCAACGAACAGGCAAAC ATCCACCCATTCCTCTACACAGATCTGTACCCTTGCCAGCACATTGGCTAAAGATACACA AGTGGCACAACCACCGACTGCAACAAAAAAGGCCTTCCTGGTAACCTTTAGGGCACAAAT AGTTACACCCATACATTTTACTCCGTGCTCATTTCAAAGAACCCTACACTAAACACCATT TGGCTCTAAGTCAAAACGGCTAATTTGCTTTCAGGCCCGAAGGGTCAACCCTTAAAGAAA ACTGTACTCTTATATGCCCCCGTACACTTTCCGTGGGGCGAACAAAAACATTACGGCATC CT |
Restriction Sites | NotI-NotI |
ACCN | NM_001254 |
Insert Size | 2870 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_001254.3, NP_001245.1 |
RefSeq Size | 3053 bp |
RefSeq ORF | 1683 bp |
Locus ID | 990 |
UniProt ID | Q99741 |
Cytogenetics | 17q21.2 |
Domains | AAA |
Protein Pathways | Cell cycle |
Summary | The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008] |
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SDS |
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.