DAGK (DGKQ) (NM_001347) Human Untagged Clone

CAT#: SC119320

DGKQ (untagged)-Human diacylglycerol kinase, theta 110kDa (DGKQ)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DAGK
• Synonyms: DAGK; DAGK4; DAGK7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001347 edited
  CGAGGCGGCAGTGTAGCGGCGGCTCCTGTACCCCCGGCGCGGACCTAAAGGGGCTCGGGC
  CGCTCGGGCCGGGAATGGCGGCGGCGGCCGAGCCCGGGGCCCGCGCCTGGCTGGGCGGCG
  GCTCCCCGCGCCCCGGCAGCCCGGCCTGCAGCCCCGTGCTGGGCTCAGGAGGCCGCGCGC
  GCCCGGGGCCGGGGCCGGGGCCGGGACCCGAGCGGGCGGGCGTCAGAGCCCCGGGCCCCG
  CTGCCGCGCCGGGACACAGCTTCCGGAAGGTGACGCTCACCAAGCCCACCTTCTGCCACC
  TCTGCTCCGACTTCATCTGGGGGCTGGCCGGCTTCCTGTGCGACGTCTGCAATTTCATGT
  CTCATGAGAAGTGCCTGAAGCACGTGAGGATCCCGTGCACGAGTGTGGCACCCAGCCTGG
  TCCGGGTTCCTGTAGCCCACTGCTTCGGCCCCCGGGGGCTCCACAAGCGCAAGTTCTGTG
  CTGTCTGCCGCAAGGTCCTGGAGGCACCGGCGCTCCACTGCGAAGTGTGTGAGCTGCACC
  TCCACCCAGACTGTGTGCCCTTCGCCTGCAGTGACTGCCGCCAGTGCCACCAGGATGGGC
  ACCAGGATCACGACACCCATCACCACCACTGGCGGGAGGGGAACCTGCCCTCGGGAGCGC
  GCTGCGAGGTCTGCAGGAAGACGTGCGGCTCCTCTGACGTGCTGGCCGGCGTGCGCTGCG
  AGTGGTGCGGGGTCCAGGCGCACTCCCTCTGCTCCGCGGCGCTGGCTCCCGAGTGTGGCT
  TCGGGCGTCTGCGCTCCCTGGTCCTGCCTCCCGCGTGCGTGCGCCTTCTGCCCGGCGGCT
  TCAGCAAGACGCAGAGCTTCCGCATCGTGGAGGCCGCGGAGCCGGGCGAGGGGGGCGACG
  GCGCCGACGGGAGCGCTGCCGTGGGTCCAGGCAGAGAGACACAGGCAACTCCGGAGTCCG
  GGAAGCAAACGCTGAAGATCTTTGATGGCGACGACGCGGTGAGAAGAAGCCAGTTCCGCC
  TCGTTACGGTGTCCCGCCTGGCCGGTGCCGAGGAGGTGCTGGAGGCCGCACTGCGGGCCC
  ACCACATCCCCGAGGACCCTGGCCACCTGGAGCTGTGCCGGCTGCCCCCTTCCTCTCAGG
  CCTGTGACGCCTGGGCTGGGGGCAAGGCTGGGAGTGCTGTGATCTCGGAGGAGGGCAGAA
  GCCCCGGGTCCGGCGAGGCCACGCCAGAGGCCTGGGTCATCCGGGCTCTGCCGCGGGCCC
  AGGAGGTCCTGAAGATCTACCCTGGCTGGCTCAAGGTGGGCGTGGCCTACGTGTCCGTGC
  GAGTGACCCCGAAGAGCACGGCCCGCTCTGTGGTGCTGGAGGTCCTGCCGCTGCTCGGCC
  GCCAGGCCGAGAGTCCCGAGAGCTTCCAGCTGGTGGAGGTGGCGATGGGCTGCAGGCACG
  TCCAGCGGACGATGCTGATGGACGAACAGCCCCTGCTGGACCGGCTACAGGACATCCGGC
  AGATGTCTGTGCGGCAGGTGAGCCAGACGCGGTTCTACGTGGCAGAGAGCAGGGATGTAG
  CCCCGCACGTCTCCCTGTTTGTTGGCGGCCTGCCTCCCGGCCTGTCTCCCGAGGAGTACA
  GCAGCCTGCTGCATGAGGCCGGGGCTACCAAAGCCACCGTGGTGTCCGTGAGTCACATCT
  ACTCCTCCCAAGGCGCGGTAGTGTTGGACGTTGCCTGCTTTGCGGAGGCCGAGCGGCTGT
  ACATGCTGCTGAAGGACATGGCTGTGCGGGGCCGGCTGCTCACTGCCCTGGTGCTCCCCG
  ACCTGCTGCACGCGAAGCTGCCCCCAGACAGCTGTCCCCTCCTTGTGTTCGTGAACCCCA
  AGAGTGGAGGCCTCAAGGGCCGAGACCTGCTCTGCAGCTTCCGGAAGCTACTGAACCCTC
  ATCAGGTCTTCGACCTGACCAACGGAGGTCCTCTTCCCGGGCTCCACCTGTTCTCCCAGG
  TGCCCTGCTTCCGGGTGCTGGTGTGTGGTGGCGATGGCACTGTGGGCTGGGTGCTTGGCG
  CCCTGGAGGAGACACGGTACCGACTGGCCTGCCCGGAGCCTTCTGTGGCCATCCTGCCCC
  TGGGCACAGGGAATGACCTTGGTCGAGTCCTCCGCTGGGGGGCGGGCTACAGCGGCGAGG
  ACCCGTTCTCCGTACTGCTGTCTGTGGACGAGGCCGACGCCGTGCTCATGGACCGCTGGA
  CCATCCTGCTGGATGCCCACGAGGCTGGCAGTGCAGAGAACGACACGGCAGACGCAGAGC
  CCCCCAAGATCGTGCAGATGAGTAACTACTGTGGCATTGGCATCGACGCGGAGCTGAGCC
  TGGACTTCCACCAGGCACGGGAAGAGGAGCCTGGCAAGTTCACAAGCAGGCTGCACAACA
  AGGGTGTGTACGTGCGGGTGGGGCTGCAGAAGATCAGTCACTCTCGGAGCCTGCACAAGC
  AGATCCGGCTGCAGGTGGAGCGGCAGGAGGTGGAGCTGCCCAGTATTGAAGGCCTCATCT
  TCATCAACATCCCCAGCTGGGGCTCGGGGGCCGACCTGTGGGGCTCCGACAGCGACACCA
  GGTTTGAGAAGCCACGCATGGACGACGGGCTGCTGGAGGTTGTGGGCGTGACGGGCGTCG
  TGCACATGGGCCAGGTCCAGGGTGGGCTGCGCTCCGGAATCCGGATTGCCCAGGGTTCCT
  ACTTCCGAGTCACGCTCCTCAAGGCCACCCCGGTGCAGGTGGACGGGGAGCCCTGGGTCC
  AGGCCCCGGGGCACATGATCATCTCAGCTGCTGGCCCTAAGGTGCACATGCTGAGGAAGG
  CCAAGCAGAAGCCGAGGAGGGCCGGGACCACCAGGGATGCCCGGGCGGATGCTGCGCCTG
  CCCCTGAGAGCGATCCTAGGTAGGGGTGGCTGGGGCAGCCCAAGGGCTCGAGCCATCTCT
  GCTCCCGCCAGCCTTGTTTTCAGGTGGTCTGGAGGCAGCTCCACGTCCACACAGTGGCCA
  GCC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001347 unedited
  NGGGTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCGGCAGTG
  TAGCGGCGGCTCCTGTACCCCCGGCGCGGACCTAAAGGGCTCGGGCCGCTCGGGCCGGGA
  ATGGCGGCGGCGGCCGAGCCCGGGGCCCGCGCCTGGCTGGGCGGCGGCTCCCCGCGCCCC
  GGCAGCCCGGCCTGCAGCCCCGTGCTGGGCTCAGGAGGCCGCGCGCGCCCGGGGCCGGGG
  CCGGGGCCGGGACCCGAGCGGGCGGGCGTCAGAGCCCCGGGCCCCGCTGCCGCGCCGGGA
  CACAGCTTCCGGAAGGTGACGCTCACCAAGCCCACCTTCTGCCACCTCTGCTCCGACTTC
  ATCTGGGGGCTGGCCGGCTTCCTGTGCGACGTCTGCAATTTCATGTCTCATGAGAAGTGC
  CTGAAGCACGTGAGGATCCCGTGCACGAGTGTGGCACCCAGCCTGGTCCGGGTTCCTGTA
  GCCCACTGCTTCGGCCCCCGGGGGCTCCACAAGCGCAAGTTCTGTGCTGTCTGCCGCAAG
  GTCCTGGAGGCACCGGCGCTCCACTGCGAAGTGTGTGAGCTGCACCTCCACCCAGACTGT
  GTGCCCTTCGCCTGCAGTGACTGCCGCCAGTGCCACCAGGATGGGCACCAGGATCACGAC
  ACCCATCACCACCACTGGCGGGGAGGGGGAACCTGCCCTCGGGAGCGCGCTGCGAGGTCT
  GCAGGAAGACGTGCGGCTCCTCTGACGTGCTGGGCCGCGTGCGCTGCAAGTGGTGGCGGG
  GTCCAAGCGCACTCCCTCTGCTCCGCGGCGCTGGCTCCCCGAGTGTGGCTTCGGGCGTCT
  GCGCTCCCTGGTCCTGCCTCCCGCGTGCGTGCGCCCNCTGCCCG
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001347 unedited
  TAAGCGGAGTTCCAACANAACCGCGNATGCCGGGNCCACCAGGCATGCCCGGGCGGCTGC
  TGCGCCTGCCCCTGAGAGCGATCCTAGGTAGGGGTGGCTGGGGCAGCCCAAGGCTCGAGC
  CATCTCTGCTCCCGCCAGCCTTGTTTTCAGGTGGTCTGGAGGCAGCTCCACGTCCACACA
  GTGGCCAGCCCTGTGGTCGGACCTGGCTACAGCCCTGACCGCGGCATCTTCCCAGTGACG
  GAGCGGGACTAGCCCTCATCCCATTGGTGACACATGTTGGTGTCACAGCCCTCATCCCAC
  TGGAGACTGGTGTGCGGGTGGCAGTGTTACCCCGTGTCCCGGGTGGGCGCAGGGCTCTGG
  ATGGTGAGCCCTCTGCCCTGTGGGGGCCTGGTCCTCTCCTCAGGGGTGGGCACCCATCCC
  TCTCCCCACTGAGCCCCTTGAGCTGGACAGTGCTGGATGCAGGCCGTCCAGGTGGGTCTG
  ATCTCACTTTGTGCCCCTCGGGCCGTCCCCCTGGCCAGTCCTTCAGAGAGGCAGGCTCCC
  TGGTGTGTCCCTGCCCCAGCAGACACAGGCTGCCAGTCCCTCCAGCCTCACCTCCCCGCC
  CTGGGCAGCAGGTGTGGGCAGCGTGTGAGGCTGCCGGGTGGTGGTGACTGGTGACTTCCT
  TGTGTTCAGGGAAGGGAGAAGGTGCCTTCCCTTGTTTTCTGGCCTTGTTATATACAGATG
  GCAGCTTGGATCTCAGGTACAGCTCCAGGGGCAGGCAGTGCCCAGCTGGACCTGGTGGCC
  CTTTCCTAGTGCCTCTGCTGGGGGGAGGAGACCTCTGTTCACGTGGAGGCTAGGAGGTNC
  TTCAGGTGCTGCCCTGGCAGCACCAAAGTGTGGGCCGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001347
• Insert Size: 5130 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001347.2, NP_001338.2
• RefSeq Size: 4653 bp
• RefSeq ORF: 2829 bp
• Locus ID: 1609
• UniProt ID: P52824
• Cytogenetics: 4p16.3
• Domains: RA, DAGKa, DAGKc, DAG_PE-bind
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008]