Cathepsin V (CTSV) (NM_001333) Human Untagged Clone

SKU SC119311

CTSL2 (untagged)-Human cathepsin L2 (CTSL2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Cathepsin V
• Synonyms: CATL2; CTSL2; CTSU
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001333, the custom clone sequence may differ by one or more nucleotides; ; ; ATGAATCTTTCGCTCGTCCTGGCTGCCTTTTGCTTGGGAATAGCCTCCGCTGTTCCAAAATTTGACCAAA; ATTTGGATACAAAGTGGTACCAGTGGAAGGCAACACACAGAAGATTATATGGCGCGAATGAAGAAGGATG; GAGGAGAGCAGTGTGGGAAAAGAATATGAAAATGATTGAACTGCACAATGGGGAATACAGCCAAGGGAAA; CATGGCTTCACAATGGCCATGAATGCTTTTGGTGACATGACCAATGAAGAATTCAGGCAGATGATGGGTT; GCTTTCGAAACCAGAAATTCAGGAAGGGGAAAGTGTTCCGTGAGCCTCTGTTTCTTGATCTTCCCAAATC; TGTGGATTGGAGAAAGAAAGGCTACGTGACGCCAGTGAAGAATCAGAAACAGTGTGGTTCTTGTTGGGCT; TTTAGTGCGACTGGTGCTCTTGAAGGACAGATGTTCCGGAAAACTGGGAAACTTGTCTCACTGAGCGAGC; AGAATCTGGTGGACTGTTCGCGTCCTCAAGGCAATCAGGGCTGCAATGGTGGCTTCATGGCTAGGGCCTT; CCAGTATGTCAAGGAGAACGGAGGCCTGGACTCTGAGGAATCCTATCCATATGTAGCAGTGGATGAAATC; TGTAAGTACAGACCTGAGAATTCTGTTGCTAATGACACTGGCTTCACAGTGGTCGCACCTGGAAAGGAGA; AGGCCCTGATGAAAGCAGTCGCAACTGTGGGGCCCATCTCCGTTGCTATGGATGCAGGCCATTCGTCCTT; CCAGTTCTACAAATCAGGCATTTATTTTGAACCAGACTGCAGCAGCAAAAACCTGGATCATGGTGTTCTG; GTGGTTGGCTACGGCTTTGAAGGAGCAAATTCGAATAACAGCAAGTATTGGCTCGTCAAAAACAGCTGGG; GTCCAGAATGGGGCTCGAATGGCTATGTAAAAATAGCCAAAGACAAGAACAACCACTGTGGAATCGCCAC; AGCAGCCAGCTACCCCAATGTGTGA; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001333 unedited; GCAGGTTTNGTAACCCCACCTCGAGTGTGGCGTTAAAAACATCCCCCACGAGGTAATCTC; ATTTCTTGTTTGCTGATGGTGCCTGCGCCCCTGCGACGGCTGCTGGTTTTGAAACATGAA; TCTTTCGCTCGTCCTGGCTGCCTTTTGCTTGGGAATAGCCTCCGCTGTTCCAAAATTTGA; CCAAAATTTGGATACAAAGTGGTACCAGTGGATTGTTTACACAGAAGATTATATGGCGCG; AATGAAGAATGATGGAGGAGAGCAGTGTGGGAAAAGAATATGAAAATGATTGAACTGCAC; AATGGGGAATACAGCCAAGGGAAACATGGCTTCACAATGGCCATGAATGCTTTTGGTGAC; ATGACCAATGAAGAATTCATGCAGATGATGGGTTGCTTTCGAAACCAGAAATTCAGGAAG; GGGAAAGTGTTCCGTGAGCCTCTGTTTCTTGATCTTCCCAAATCTGTGGATTGGAGAAAG; AAAGGCTACGTGACGCCAGTGAAGAATCAGAAACAGTGTGGTTCTTGTTGGGCTTTTAGT; GCGACTGGTGCTCTTGAAGGACAGATGTTCCGGATAACTGGGAAACTTGTCTCACTGAGC; GAGCAGAATCTGGTGGACTGTTCGCGTCCTCAAGGCAATCATGGCTGCAATGGTGGCTTC; ATGGCTAGGGCCTTCCAGTATGTCAAGGAGAACGGAGGCCTGGACTCTGAGGAATCCTAT; CCATATGTAGCAGTGGATGATATCTGTGAGTACAGACCTGAGAATTCTGTTGCTAATGAC; ACTGGCTTCACAGTGGTCGCACCTGGAAATGAGTAGGCCCTGATGAAAGCAGTCGCACTG; TGGGGCCCTTCTTCCGTGCTATGGATGCAGGCCATTCGTCCTTCCAGGTCTACAAATCAG; GCATTTATTTTGGACCAGACTGCAGCACTAAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001333 unedited; CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA; TATACGAATTAAAATTTATTTCAAACTGTTTTGTACATCTTTTTAAAAAATGAAAATTCA; AAAGTCTTAAAATTAAGCAATGAGTCTTTGATATCATAAAGCTGGGTATAACAATAATTA; AAGTAGGGGTAACATTTTACCCTTGTAAAAATGTCACAAAATTAAAATCTCAACTTGGAT; CCTCAATGATTCAACTGGTTTATCTTACACAATAAGCGTTTGGTCAGTTTCAAAATAAAA; TTTCCCCAAACATGCTGGCCTTAAGTCCTTCCTCCTCACCATCCATCAGCTCACACATTG; GGGTAGCTGGCTGCTGTGGCGATTCCACAGGGGGTGGTCTTGGCTTTGGCTATTTTTACA; TAGCCATTCGAGCCCCATTCTGGACCCCAGCTGTTTTTGACGAGCCAATACTTGCTGTTA; TTCGAATTTGCTCCTTCAAAGCCGTAGCCAACCACCAGAACACCATGATCCAGGTTTTTG; CTGCTGCAGTCTGGTTCAAAATAAATGCCTGATTTGTAGAACTGGAAGGACGAATGGCCT; GCATCCATAGCAACGGAGATGGGCCCCACAGTTGCGACTGCTTTCATCAGGGCCTTCTCC; TTTCCAGGTGCGACCACTGTGAAGCCAGTGTCATTACCAACAGATTCTAGGTCTGTACTA; CAGATTCATCCCTGCTACTATGGTAGGATTCCTCAGATCCAAGCCTCCGTTTCCTGACTC; TGAAAGCCTACCCTGAAGCCCCTTGCAACCTGATGCCTGGGACCGAACATCACCAATTTG; TTCGTATGAAACAGTTCCAGTTTCGAAAATTGTCTAAGAGCCCGGCGACTAAGCCACAGA; CCCTTGTTGATTTCCGGGGCCGT;
• Restriction Sites: NotI-NotI
• ACCN: NM_001333
• Insert Size: 1350 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001333.2, NP_001324.2
• RefSeq Size: 1496 bp
• RefSeq ORF: 1005 bp
• Locus ID: 1515
• UniProt ID: O60911
• Cytogenetics: 9q22.33
• Domains: Pept_C1
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Lysosome
• Summary: The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2011]; Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.