DDX3 (DDX3X) (NM_001356) Human Untagged Clone

SKU SC119279

DDX3X (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked (DDX3X), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: DDX3
• Synonyms: CAP-Rf; DBX; DDX3; DDX14; HLP2; MRX102; MRXSSB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119279 sequence for NM_001356 edited (data generated by NextGen Sequencing); ATGAGTCATGTGGCAGTGGAAAATGCGCTCGGGCTGGACCAGCAGTTTGCTGGCCTAGAC; CTGAACTCTTCAGATAATCAGAGTGGAGGAAGTACAGCCAGCAAAGGGCGCTATATTCCT; CCTCATTTAAGGAACCGAGAAGCTACTAAAGGTTTCTACGATAAAGACAGTTCAGGGTGG; AGTTCTAGCAAAGATAAGGATGCGTATAGCAGTTTTGGATCTCGTAGTGATTCAAGAGGG; AAGTCTAGCTTCTTCAGTGATCGTGGAAGTGGATCAAGGGGAAGGTTTGATGATCGTGGA; CGGAGTGATTACGATGGCATTGGCAGCCGTGGTGACAGAAGTGGCTTTGGCAAATTTGAA; CGTGGTGGAAACAGTCGCTGGTGTGACAAATCAGATGAAGATGATTGGTCAAAACCACTC; CCACCAAGTGAACGCTTGGAACAGGAACTCTTTTCTGGAGGCAACACTGGGATTAATTTT; GAGAAATACGATGACATTCCAGTTGAGGCAACAGGCAACAACTGTCCTCCACATATTGAA; AGTTTCAGTGATGTTGAGATGGGAGAAATTATCATGGGAAACATTGAGCTTACTCGTTAT; ACTCGCCCAACTCCAGTGCAAAAGCATGCTATTCCTATTATCAAAGAGAAAAGAGACTTG; ATGGCTTGTGCCCAAACAGGGTCTGGAAAAACTGCAGCATTTCTGTTGCCCATCTTGAGT; CAGATTTATTCAGATGGTCCAGGCGAGGCTTTGAGGGCCATGAAGGAAAATGGAAGGTAT; GGGCGCCGCAAACAATACCCAATCTCCTTGGTATTAGCACCAACGAGAGAGTTGGCAGTA; CAGATCTACGAGGAAGCCAGAAAATTTTCATACCGATCTAGAGTTCGTCCTTGCGTGGTT; TATGGTGGTGCCGATATTGGTCAGCAGATTCGAGACTTGGAACGTGGATGCCATTTGTTA; GTAGCCACTCCAGGACGTCTAGTGGATATGATGGAAAGAGGAAAGATTGGATTAGACTTT; TGCAAATACTTGGTGTTAGATGAAGCTGATCGGATGTTGGATATGGGGTTTGAGCCTCAG; ATTCGTAGAATAGTCGAACAAGATACTATGCCTCCAAAGGGTGTCCGCCACACTATGATG; TTTAGTGCTACTTTTCCTAAGGAAATACAGATGCTGGCTCGTGATTTCTTAGATGAATAT; ATCTTCTTGGCTGTAGGAAGAGTTGGCTCTACCTCTGAAAACATCACACAGAAAGTAGTT; TGGGTGGAAGAATCAGACAAACGGTCATTTCTGCTTGACCTCCTAAATGCAACAGGCAAG; GATTCACTGACCTTAGTGTTTGTGGAGACCAAAAAGGGTGCAGATTCTCTGGAGGATTTC; TTATACCATGAAGGATACGCATGTACCAGCATCCATGGAGACCGTTCTCAGAGGGATAGA; GAAGAGGCCCTTCACCAGTTCCGCTCAGGAAAAAGCCCAATTTTAGTGGCTACAGCAGTA; GCAGCAAGAGGACTGGACATTTCAAATGTGAAACATGTTATCAATTTTGACTTGCCAAGT; GATATTGAAGAATATGTACATCGTATTGGTCGTACGGGACGTGTAGGAAACCTTGGCCTG; GCAACCTCATTCTTTAACGAGAGGAACATAAATATTACTAAGGATTTGTTGGATCTTCTT; GTTGAAGCTAAACAAGAAGTGCCGTCTTGGTTAGAAAACATGGCTTATGAACACCACTAC; AAGGGTAGCAGTCGTGGACGTTCTAAGAGTAGCAGATTTAGTGGAGGGTTTGGTGCCAGA; GACTACCGACAAAGTAGCGGTGCCAGCAGTTCCAGCTTCAGCAGCAGCCGCGCAAGCAGC; AGCCGCAGTGGCGGAGGTGGCCACGGTAGCAGCAGAGGATTTGGTGGAGGTGGCTATGGA; GGCTTTTACAACAGTGATGGATATGGAGGAAATTATAACTCCCAGGGGGTTGACTGGTGG; GGTAACTGA; ; Clone variation with respect to NM_001356.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001356 unedited; GGGATATTTGAAAACCACGCACTTTCACTATAGGGCGGCACGCGCAATTCGGCACGAGGC; GTGAGAGGGCCTTCGCGGTGGAACAAACACTCGCTTAGCAGCGGAAGACTCCGAGTTCTC; GGTACTCTTCAGGGATGAGTCATGTGGCAGTGGAAAATGCGCTCGGGCTGGACCAGCAGT; TTGCTGGCCTAGACCTGAACTCTTCAGATAATCAGAGTGGAGGAATTACAGCCAGCAAAG; GGCGCTATATTCCTCCTCATTTAAGGAACCGAGAAGCTACTAAAGGTTTCTACGATAAAG; ACAGTTCAGGGTGGAGTTCTAGCAAAGATAAGGATGCGTATAGCAGTTTTGGATCTCGTA; GTGATTCAAGAGGGAAGTCTAGCTTCTTCAGTGATCGTGGAAGTGGATCAAGGGGAAGGT; TTGATGATCGTGGACGGAGTGATTACGATGGCATTGGCAGCCGTGGTGACAGAAGTGGCT; TTGGCAAATTTGAACGTGGTGGAAACAGTCGCTGGTGTGACAAATCAGATGAAGATGATT; GGTCAAAACCACTCCCACCAAGTGAACGCTTGGAACAGGAACTCTTTTCTGGAGGCAACA; CTGGGATTAATTTTGAGAAATACGATGACATTCCAGTTGAGGCAACAGGCAACAACTGTC; CTCCACATATTGAAAGTTTCAGTGATGTTGAGATGGGAGAAATTATCATGGGAAACATTG; AGCTTACTCGTTATACTCGCCCAACTCCAGTGCAAAAGCATGCTATTCCTATTATCAAAG; AGAAAAGAGACTTGATGGCTTGTGCCCAAACAGGGTCTGGAAAAACTGCAGCATTTCTGG; TGCCCATCTTGAGTCAGATTTATTCAGATGGTCCAGGCGAGGCTTTGAGGCCATGAANGA; NATGNANGTATGGNCGCGCAACATC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001356 unedited; CTGGGCATGGCATGGCAACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGGCAG; CCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTT; TTTTTTTTTTTTTTCAGGCAAGAGCACTTTATTTACACCTCTGAAAATAAACCACAATTG; ACAACAACAAAGGAGACTGTGCAGTTCAACTTTTTATTTTAATAAAATCAGAATATGCAC; AGCACATGCAGTGCTAGCATCTAAACTAATACAATAAGCAATTACTAAAGCTACAGTGAC; TTGAGGTTCAATCTTTACATTCAGCAAGTTTAAATCCTTACATGATTTTTGAAACCTTAT; TAACTGAAAATATTGCTTGCTGATAAAACTTGCTCAAATGCTATTGCTGAATGTACAAGT; CACTGATTATGCCAATACAACAAAGATAACCACATGTTTGAGGATTGCAATGTGCCAGAC; ATTCACTAAAAAAAAAAAAAAAACACACAACTAAACAAAATCCACACAACAAACATCTGA; GAATCTGGACACTTCACATCAGTGTTTTGAAGTGTTTCATTAATATCTTAAGACAAGTAT; ATCAAAACCTTGGCATGATTTAATTTCAAGTCGCCAATTTTGTTTTTACTAACATCAAAA; AGTTATGGCTACACTGCCAACGCCGGGTCTGCTTAATTTGACTTAAGAATTTAATATGAC; TTAAACATTAAAAGCTCCACTCCCCCGAAATATAAAATTTCCCGCGAACGGGGAACATTA; CACATTCAAGTGCCCAGTGTTTTTGAACCTGGCTTTTGGGGCAAGTTTTCCTTAAAATTT; CCAAGAAAGAACGTTTAGGCTTAAAAAATAAAAATTGGCTCAGAGGTCGCAAAATTTAGC; AAAACAACCGGCCAAAGTGTCTGGAGACCTGCGGGCGAAAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001356
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001356.2, NP_001347.2
• RefSeq Size: 5322 bp
• RefSeq ORF: 1989 bp
• Locus ID: 1654
• UniProt ID: O00571
• Cytogenetics: Xp11.4
• Domains: DEAD, helicase_C
• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: RIG-I-like receptor signaling pathway
• Summary: The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).