EIF4B (NM_001417) Human Untagged Clone

SKU SC119269

EIF4B (untagged)-Human eukaryotic translation initiation factor 4B (EIF4B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: EIF4B
• Synonyms: EIF-4B; PRO1843
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119269 sequence for NM_001417 edited (data generated by NextGen Sequencing); ATGGCGGCCTCAGCAAAAAAGAAGAATAAGAAGGGGAAGACTATCTCCCTAACAGACTTT; CTGGCTGAGGATGGGGGTACTGGTGGAGGAAGCACCTATGTTTCCAAACCAGTCAGCTGG; GCTGATGAAACGGATGACCTGGAAGGAGATGTTTCGACCACTTGGCACAGTAACGATGAC; GATGTGTATAGGGCGCCTCCAATTGACCGTTCCATCCTTCCCACTGCTCCACGGGCTGCT; CGGGAACCCAATATCGACCGGAGCCGTCTTCCCAAATCGCCACCCTACACTGCTTTTCTA; GGAAACCTACCCTATGATGTTACAGAAGAGTCAATTAAGGAATTCTTTCGAGGATTAAAT; ATCAGTGCAGTGCGTTTACCACGTGAACCCAGCAATCCAGAGAGGTTGAAAGGTTTTGGT; TATGCTGAATTTGAGGACCTGGATTCCCTGCTCAGTGCCCTGAGTCTCAATGAAGAGTCT; CTAGGTAACAGGAGAATTCGAGTGGACGTTGCTGATCAAGCACAGGATAAAGACAGGGAT; GATCGTTCTTTTGGCCGTGATAGAAATCGGGATTCTGACAAAACAGATACAGACTGGAGG; GCTCGTCCTGCTACAGACAGCTTTGATGACTACCCACCTAGAAGAGGTGATGATAGCTTT; GGAGACAAGTATCGAGATCGTTATGATTCAGACCGGTATCGGGATGGGTATCGGGATGGG; TATCGGGATGGCCCACGCCGGGATATGGATCGATATGGTGGCCGGGATCGCTATGATGAC; CGAGGCAGCAGAGACTATGATAGAGGCTATGATTCCCGGATAGGCAGTGGCAGAAGAGCA; TTTGGCAGTGGGTATCGCAGGGATGATGACTACAGAGGAGGCGGGGACCGCTATGAAGAC; CGATATGACAGACGGGATGATCGGTCGTGGAGCTCCAGAGATGATTACTCTCGGGATGAT; TATAGGCGTGATGATAGAGGTCCCCCCCAAAGACCCAAACTGAATCTAAAGCCTCGGAGT; ACTCCTAAGGAAGATGATTCCTCTGCTAGTACCTCCCAGTCCACTCGAGCTGCTTCTATC; TTTGGAGGGGCAAAGCCTGTTGACACAGCTGCTAGAGAAAGAGAAGTAGAAGAACGGCTA; CAGAAGGAACAAGAGAAGTTGCAGCGTCAGCTGGATGAGCCAAAACTAGAACGACGGCCT; CGGGAGAGACACCCAAGCTGGCGAAGTGAAGAAACTCAGGAACGGGAACGGTCGAGGACA; GGAAGTGAGTCATCACAAACTGGGACCTCCACCACATCTAGCAGAAATGCACGAAGGAGA; GAGAGTGAGAAGTCTCTAGAAAATGAAACACTCAATAAGGAGGAAGATTGCCACTCTCCA; ACTTCTAAACCTCCCAAACCTGATCAGCCCCTAAAGGTAATGCCAGCCCCTCCACCAAAG; GAGAATGCTTGGGTGAAGCGAAGTTCTAACCCTCCTGCTCGATCTCAGAGCTCAGACACA; GAGCAGCAGTCCCCTACAAGTGGTGGGGGAAAAGTAGCTCCAGCTCAACCATCTGAGGAA; GGACCAGGAAGGAAAGATGAAAATAAAGTAGATGGGATGAATGCCCCAAAAGGCCAAACT; GGGAACTCTAGCCGTGGTCCAGGAGACGGAGGGAACAGAGACCACTGGAAGGAGTCAGAT; AGGAAAGATGGCAAAAAGGATCAAGACTCCAGATCTGCACCTGAGCCAAAGAAACCTGAG; GAAAATCCAGCTTCYAAGTTCAGTTCTGCAAGCAAGTATGCTGCTCTCTCTGTTGATGGT; GAAGATGAAAATGAGGGAGAAGATTATGCCGAATAG; ; Clone variation with respect to NM_001417.4; 1755 c=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001417 unedited; NGGCGTGTCATATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGCTCCC; AACATGGCGGCCTCAGCAAAAAAGAAGAATAAGAAGGGGAAGACTATCTCCCTAACAGAC; TTTCTGGCTGAGGATGGGGGTACTGGTGGAGGAAGCACCTATGTTTCCAAACCAGTCAGC; TGGGCTGATGAAACGGATGACCTGGAAGGAGATGTTTCGACCACTTGGCACAGTAACGAT; GACGATGTGTATAGGGCGCCTCCAATTGACCGTTCCATCCTTCCCACTGCTCCACGGGCT; GCTCGGGAACCCAATATCGACCGGAGCCGTCTTCCCAAATCGCCACCCTACACTGCTTTT; CTAGGAAACCTACCCTATGATGTTACAGAAGAGTCAATTAAGGAATTCTTTCGAGGATTA; AATATCAGTGCAGTGCGTTTACCACGTGAACCCAGCAATCCAGAGAGGTTGAAAGGTTTT; GGTTATGCTGAATTTGAGGACCTGGATTCCCTGCTCAGTGCCCTGAGTCTCAATGAAGAG; TCTCTAGGTAACAGGAGAATTCGAGTGGACGTTGCTGATCAAGCACAGGATAAAGACAGG; GATGATCGTTCTTTTGGCCGTGATAGAAATCGGGATTCTGACAAAACAGATACAGACTGG; AGGGCTCGTCCTGCTACAGACAGCTNTGATGACTACCCACCTAGAAGAGGTGATGATAGC; TNTGGAGACAAGTATCGAGATCGTTATGATTCAGACCGGTATCGGGATGGGTATCGGNAT; GGGGTATCGGATGGCCCACGCCGGGATATGGATCGATATGGTGGCCCGGNATCGCTATGA; TGACCCGAGCAGCAGAGACTATGATAGAGGCTATGATTCCCGNATAGCAGTGGCAGAAGA; GCATTTGNCA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001417 unedited; CTATGTACGCGGCCGCAATCTATAGTCGAGTNTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTAAAGAAAAGGGGGATTTTTATTTTGTCTTGTCGGAATA; AAGGTTTTGATTTGCTCTCAAATGTCCCAGGGGGGAAAAAACCTAGGAAAAAAACCCCAG; GATGTAAAGGCCTTTTCGGCATAATCTTCCCCCTCTTTTTCTTCTTCCCCATCAACAAAA; AAACCACCTTCCTTGCTTGCAAAACTGAACTTGAAAGCTGGATTTTCCTCAGGTTTTTTT; GGCTCAGGGGCAAATCGGGAGTCTTGACCCTTTTTGCCATCTTTCCTATCTGACTCCTTC; CAGGGGTCTTTGTCCCCTCCGTCTCCTGAACCACGGTTAAATTTCCCATTTGGGCCTTTG; GGGGCTTTCACCCCATCAACTTTATTTTCATCTTTCCTTCCGGGCCCTTCCTAAAATGGG; GGAGCGGGACCTACTTTTCCCCCACCACTTGTAGGGAACTGCTGCTCTGGGTCTGACCTC; TGAAATCAACCAGGAGGGTTAAAACCTTCCTTTCCCCAACCATTCTCCTTTGGGGGAGGG; GCGGGCTTTACCTTTAGGGGCTGACCAGGTTGGGAAGGTCTAAAATTCGGAAATGGGCAT; CCTCCCCCTTTTGGAGGGTTAATTTCCTAGAAACTTTCCCTCCTTTCCTCTTCGGCTTTC; TGCAAATGGGGGGGAGGCCCCCGTCTGGGATACCCCCTACTTGTCCCAACCGCCCCGTAC; TCGATTTCTTCCCTACACCATTTGCGTGTACACTCCACGTCCTCTCTTACTACCTCTCTC; CCCCTCTCCACGCTCTCTTCTCACTTCCTCACCCTCTCACCACCCTCTTTGCCCCATCCT; TTCTTTTCTTATCCCCACCCCCCCTCCCTATCTCTCTTTCACCCACACCACCCCACTTCC; TCTCATCCTCTCATACCTCTT;
• Restriction Sites: NotI-NotI
• ACCN: NM_001417
• Insert Size: 2090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001417.2, NP_001408.2
• RefSeq Size: 3919 bp
• RefSeq ORF: 1836 bp
• Locus ID: 1975
• UniProt ID: P23588
• Cytogenetics: 12q13.13
• Domains: RRM
• Protein Pathways: mTOR signaling pathway
• Summary: Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.