EIF4B (NM_001417) Human Untagged Clone

CAT#: SC119269

EIF4B (untagged)-Human eukaryotic translation initiation factor 4B (EIF4B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF4B
• Synonyms: EIF-4B; PRO1843
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119269 sequence for NM_001417 edited (data generated by NextGen Sequencing)
  ATGGCGGCCTCAGCAAAAAAGAAGAATAAGAAGGGGAAGACTATCTCCCTAACAGACTTT
  CTGGCTGAGGATGGGGGTACTGGTGGAGGAAGCACCTATGTTTCCAAACCAGTCAGCTGG
  GCTGATGAAACGGATGACCTGGAAGGAGATGTTTCGACCACTTGGCACAGTAACGATGAC
  GATGTGTATAGGGCGCCTCCAATTGACCGTTCCATCCTTCCCACTGCTCCACGGGCTGCT
  CGGGAACCCAATATCGACCGGAGCCGTCTTCCCAAATCGCCACCCTACACTGCTTTTCTA
  GGAAACCTACCCTATGATGTTACAGAAGAGTCAATTAAGGAATTCTTTCGAGGATTAAAT
  ATCAGTGCAGTGCGTTTACCACGTGAACCCAGCAATCCAGAGAGGTTGAAAGGTTTTGGT
  TATGCTGAATTTGAGGACCTGGATTCCCTGCTCAGTGCCCTGAGTCTCAATGAAGAGTCT
  CTAGGTAACAGGAGAATTCGAGTGGACGTTGCTGATCAAGCACAGGATAAAGACAGGGAT
  GATCGTTCTTTTGGCCGTGATAGAAATCGGGATTCTGACAAAACAGATACAGACTGGAGG
  GCTCGTCCTGCTACAGACAGCTTTGATGACTACCCACCTAGAAGAGGTGATGATAGCTTT
  GGAGACAAGTATCGAGATCGTTATGATTCAGACCGGTATCGGGATGGGTATCGGGATGGG
  TATCGGGATGGCCCACGCCGGGATATGGATCGATATGGTGGCCGGGATCGCTATGATGAC
  CGAGGCAGCAGAGACTATGATAGAGGCTATGATTCCCGGATAGGCAGTGGCAGAAGAGCA
  TTTGGCAGTGGGTATCGCAGGGATGATGACTACAGAGGAGGCGGGGACCGCTATGAAGAC
  CGATATGACAGACGGGATGATCGGTCGTGGAGCTCCAGAGATGATTACTCTCGGGATGAT
  TATAGGCGTGATGATAGAGGTCCCCCCCAAAGACCCAAACTGAATCTAAAGCCTCGGAGT
  ACTCCTAAGGAAGATGATTCCTCTGCTAGTACCTCCCAGTCCACTCGAGCTGCTTCTATC
  TTTGGAGGGGCAAAGCCTGTTGACACAGCTGCTAGAGAAAGAGAAGTAGAAGAACGGCTA
  CAGAAGGAACAAGAGAAGTTGCAGCGTCAGCTGGATGAGCCAAAACTAGAACGACGGCCT
  CGGGAGAGACACCCAAGCTGGCGAAGTGAAGAAACTCAGGAACGGGAACGGTCGAGGACA
  GGAAGTGAGTCATCACAAACTGGGACCTCCACCACATCTAGCAGAAATGCACGAAGGAGA
  GAGAGTGAGAAGTCTCTAGAAAATGAAACACTCAATAAGGAGGAAGATTGCCACTCTCCA
  ACTTCTAAACCTCCCAAACCTGATCAGCCCCTAAAGGTAATGCCAGCCCCTCCACCAAAG
  GAGAATGCTTGGGTGAAGCGAAGTTCTAACCCTCCTGCTCGATCTCAGAGCTCAGACACA
  GAGCAGCAGTCCCCTACAAGTGGTGGGGGAAAAGTAGCTCCAGCTCAACCATCTGAGGAA
  GGACCAGGAAGGAAAGATGAAAATAAAGTAGATGGGATGAATGCCCCAAAAGGCCAAACT
  GGGAACTCTAGCCGTGGTCCAGGAGACGGAGGGAACAGAGACCACTGGAAGGAGTCAGAT
  AGGAAAGATGGCAAAAAGGATCAAGACTCCAGATCTGCACCTGAGCCAAAGAAACCTGAG
  GAAAATCCAGCTTCYAAGTTCAGTTCTGCAAGCAAGTATGCTGCTCTCTCTGTTGATGGT
  GAAGATGAAAATGAGGGAGAAGATTATGCCGAATAG
  
  Clone variation with respect to NM_001417.4
  1755 c=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001417 unedited
  NGGCGTGTCATATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGCTCCC
  AACATGGCGGCCTCAGCAAAAAAGAAGAATAAGAAGGGGAAGACTATCTCCCTAACAGAC
  TTTCTGGCTGAGGATGGGGGTACTGGTGGAGGAAGCACCTATGTTTCCAAACCAGTCAGC
  TGGGCTGATGAAACGGATGACCTGGAAGGAGATGTTTCGACCACTTGGCACAGTAACGAT
  GACGATGTGTATAGGGCGCCTCCAATTGACCGTTCCATCCTTCCCACTGCTCCACGGGCT
  GCTCGGGAACCCAATATCGACCGGAGCCGTCTTCCCAAATCGCCACCCTACACTGCTTTT
  CTAGGAAACCTACCCTATGATGTTACAGAAGAGTCAATTAAGGAATTCTTTCGAGGATTA
  AATATCAGTGCAGTGCGTTTACCACGTGAACCCAGCAATCCAGAGAGGTTGAAAGGTTTT
  GGTTATGCTGAATTTGAGGACCTGGATTCCCTGCTCAGTGCCCTGAGTCTCAATGAAGAG
  TCTCTAGGTAACAGGAGAATTCGAGTGGACGTTGCTGATCAAGCACAGGATAAAGACAGG
  GATGATCGTTCTTTTGGCCGTGATAGAAATCGGGATTCTGACAAAACAGATACAGACTGG
  AGGGCTCGTCCTGCTACAGACAGCTNTGATGACTACCCACCTAGAAGAGGTGATGATAGC
  TNTGGAGACAAGTATCGAGATCGTTATGATTCAGACCGGTATCGGGATGGGTATCGGNAT
  GGGGTATCGGATGGCCCACGCCGGGATATGGATCGATATGGTGGCCCGGNATCGCTATGA
  TGACCCGAGCAGCAGAGACTATGATAGAGGCTATGATTCCCGNATAGCAGTGGCAGAAGA
  GCATTTGNCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001417 unedited
  CTATGTACGCGGCCGCAATCTATAGTCGAGTNTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTAAAGAAAAGGGGGATTTTTATTTTGTCTTGTCGGAATA
  AAGGTTTTGATTTGCTCTCAAATGTCCCAGGGGGGAAAAAACCTAGGAAAAAAACCCCAG
  GATGTAAAGGCCTTTTCGGCATAATCTTCCCCCTCTTTTTCTTCTTCCCCATCAACAAAA
  AAACCACCTTCCTTGCTTGCAAAACTGAACTTGAAAGCTGGATTTTCCTCAGGTTTTTTT
  GGCTCAGGGGCAAATCGGGAGTCTTGACCCTTTTTGCCATCTTTCCTATCTGACTCCTTC
  CAGGGGTCTTTGTCCCCTCCGTCTCCTGAACCACGGTTAAATTTCCCATTTGGGCCTTTG
  GGGGCTTTCACCCCATCAACTTTATTTTCATCTTTCCTTCCGGGCCCTTCCTAAAATGGG
  GGAGCGGGACCTACTTTTCCCCCACCACTTGTAGGGAACTGCTGCTCTGGGTCTGACCTC
  TGAAATCAACCAGGAGGGTTAAAACCTTCCTTTCCCCAACCATTCTCCTTTGGGGGAGGG
  GCGGGCTTTACCTTTAGGGGCTGACCAGGTTGGGAAGGTCTAAAATTCGGAAATGGGCAT
  CCTCCCCCTTTTGGAGGGTTAATTTCCTAGAAACTTTCCCTCCTTTCCTCTTCGGCTTTC
  TGCAAATGGGGGGGAGGCCCCCGTCTGGGATACCCCCTACTTGTCCCAACCGCCCCGTAC
  TCGATTTCTTCCCTACACCATTTGCGTGTACACTCCACGTCCTCTCTTACTACCTCTCTC
  CCCCTCTCCACGCTCTCTTCTCACTTCCTCACCCTCTCACCACCCTCTTTGCCCCATCCT
  TTCTTTTCTTATCCCCACCCCCCCTCCCTATCTCTCTTTCACCCACACCACCCCACTTCC
  TCTCATCCTCTCATACCTCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001417
• Insert Size: 2090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001417.2, NP_001408.2
• RefSeq Size: 3919 bp
• RefSeq ORF: 1836 bp
• Locus ID: 1975
• UniProt ID: P23588
• Cytogenetics: 12q13.13
• Domains: RRM
• Protein Pathways: mTOR signaling pathway
• Gene Summary: Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.