EIF4A1 (NM_001416) Human Untagged Clone

CAT#: SC119268

EIF4A1 (untagged)-Human eukaryotic translation initiation factor 4A1 (EIF4A1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF4A1
• Synonyms: DDX2A; EIF-4A; eIF-4A-I; EIF4A; eIF4A-I
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001416, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTGCGAGCCAGGATTCCCGATCCAGAGACAATGGCCCCGATGGGATGGAGCCCGAAGGCGTCATCG
  AGAGTAACTGGAATGAGATTGTTGACAGCTTTGATGACATGAACCTCTCGGAGTCCCTTCTCCGTGGCAT
  CTACGCGTATGGTTTTGAGAAGCCCTCTGCCATCCAGCAGCGAGCCATTCTACCTTGTATCAAGGGTTAT
  GATGTGATTGCTCAAGCCCAATCTGGGACTGGGAAAACGGCCACATTTGCCATATCGATTCTGCAGCAGA
  TTGAATTAGATCTAAAAGCCACCCAGGCCTTGGTCCTAGCACCCACTCGAGAATTGGCTCAGCAGATACA
  GAAGGTGGTCATGGCACTAGGAGACTACATGGGCGCCTCCTGTCACGCCTGTATCGGGGGCACCAACGTG
  CGTGCTGAGGTGCAGAAACTGCAGATGGAAGCTCCCCACATCATCGTGGGTACCCCTGGCCGTGTGTTTG
  ATATGCTTAACCGGAGATACCTGTCCCCCAAATACATCAAGATGTTTGTACTGGATGAAGCTGACGAAAT
  GTTAAGCCGTGGATTCAAGGACCAGATCTATGACATATTCCAAAAGCTCAACAGCAACACCCAGGTAGTT
  TTGCTGTCAGCCACAATGCCTTCTGATGTGCTTGAGGTGACCAAGAAGTTCATGAGGGACCCCATTCGGA
  TTCTTGTCAAGAAGGAAGAGTTGACCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGA
  GTGGAAGCTGGACACACTATGTGACTTGTATGAAACCCTGACCATCACCCAGGCAGTCATCTTCATCAAC
  ACCCGGAGGAAGGTGGACTGGCTCACCGAGAAGATGCATGCTCGAGATTTCACTGTATCCGCCATGCATG
  GAGATATGGACCAAAAGGAACGAGACGTGATTATGAGGGAGTTTCGTTCTGGCTCTAGCAGAGTTTTGAT
  TACCACTGACCTGCTGGCCAGAGGCATTGATGTGCAGCAGGTTTCTTTAGTCATCAACTATGACCTTCCC
  ACCAACAGGGAAAACTATATCCACAGAATCGGTCGAGGTGGACGGTTTGGCCGTAAAGGTGTGGCTATTA
  ACATGGTGACAGAAGAAGACAAGAGGACTCTTCGAGACATTGAGACCTTCTACAACACCTCCATTGAGGA
  AATGCCCCTCAATGTTGCTGACCTCATCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001416 unedited
  GATTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGGATCATGTCTGCGA
  GCCAGGATTCCCGATCCAGAGACATGGCCCCGATGGGATGGAGCCCGAAGGCGTCATCGA
  GAGTAACTGGAATGAGATTGTTGACAGCTTTGATGACATGAACCTCTCGGAGTCCCTTCT
  CCGTGGCATCTACGCCTATGGTTTTGAGAAGCCCTCTGCCATCCAGCAGCGAGCCATTCT
  ACCTTGTATCAAGGGTTATGATGTGATTGCTCAAGCCCAATCTGGGACTGGGAAAACGGC
  CACATTTGCCATATCAATTCTGCAGCAGATTGAATTAGATCTAAAAGCCACCCAGGCCTT
  GGTCCTAGCACCCACTCGAGAATTGGCTCAGCAGATACAGAAGGTGGTCATGGCACTAGG
  AGACTACATGGGCGCCTCCTGTCACGCCTGTATCGGGGGCACCAACGTGCGTGCTGAGGT
  GCAGAAACTGCAGATGGAAGCTCCCCACATCATCGTGGGTACCCCTGGCCGTGTGTTTGA
  TATGCTTAACCGGNAGATACCTGTCCCCCAAATACATCAAGATGTNTTGTACTGGATGAA
  GCTGACGAAATGTTAAGCCGTGGATTCAAGGACCAGATCTATGACATATTCCAAAAGCTC
  AACAGCAACACCCAGGNTAGTTNTTGCTGTCAGCCACAATGCCTTTCTGATGTGCTTGAG
  GTGCCCAAGAAGTTCATGANGGGACCCCATTCNGNATTCTTGTCAGAAGGNAAGAGTTGA
  CCCTGGAGGGTATNCCGCCAGTTCTCCATCAACGTGAACGAANAGANTGGGAACTGGACC
  CACTATGTGACTTGAATGAACCCTGACCATACCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001416 unedited
  CCCTTGANGCCGGCGACCTCGGGGTTGAGACCTACCCCAAATAACAGGTGTCTAAAAGTT
  CCGGTTCCTTCTTTTGCCTCAAAAATGACATTTATTCAAAGAAAAAAATGACAAGATGTC
  CATCCCTTGGCTCCCTTCCCTCCCCCCTCCTGCTGCTCCTCAGCCCCCAGAGATTGAGCC
  CTGGCTGGGGCTGGGTGGCAGGACAGCCCCTCAGATGATGTCAGCAACATTGAGGGGCAT
  TTCCTCAATGGAGGTGTTGTAGAAGGTCTCAATGTCTCGAAGAGTCCTCTTGTCTTCTTC
  TGTCACCATGTTAATAGCCACACCTTTACGGCCAAACCGTCCACCTCGACCGATTCTGTG
  GATATAGTTTTCCCTGTTGGTGGGAAGGTCATAGTTGATGACTAAAGAAACCTGCTGCAC
  ATCAATGCCTCTGGCCAGCAGGTCAGTGGTAATCAAAACTCTGCTAGAGCCAGAACGAAA
  CTCCCTCATAATCACGTCTCGTTCCTTTTGGTCCATATCTCCATGCATGGCGGATACAGT
  GAAATCTCGAGCATGCATCTTCTCGGTGAGCCAGTCCACCTTCCTCCGGGTGTTGATGAA
  GATGACTGCCTGGGTGATGGTCAGGGTTTCATACAAGTCACATAGTGTGTCCAGCTTCCA
  CTCCTCTCGTTCCACGTTGATGTAGAACTGGCGGATACCCTCCAGGGTCAACTCTTCCTT
  CTTGACAAGAATCCGAATGGGGTCCCTCATGAACTTCTTGGTCACCTCAAGCACATCAGA
  AGGCATTGTGGCTGACAGCAAAACTACCTGAGTGTTGCTGNTGAGCTTTTGAATATGTCA
  TAGATCTGGGTCCTTGATCCACGGNCTTACATTTCGTCAGCTTCATCCAGTACAAACATC
  TTGATGTATTTGGGGGACAAGTATCTCCAGTTAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001416
• Insert Size: 1900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001416.1, NP_001407.1
• RefSeq Size: 1894 bp
• RefSeq ORF: 1221 bp
• Locus ID: 1973
• UniProt ID: P60842
• Cytogenetics: 17p13.1
• Domains: DEAD, helicase_C
• Gene Summary: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).