DUSP4 (NM_001394) Human Untagged Clone

CAT#: SC119258

DUSP4 (untagged)-Human dual specificity phosphatase 4 (DUSP4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DUSP4
• Synonyms: HVH2; MKP-2; MKP2; TYP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001394 edited
  GAATTCGGCACGAGGAGGAAGGGGACAGGGAAGAAGAGGCTCTCCCGCGGGAGCCCTTGA
  GGACCAAGTTTGCGGCCACTTCTGCAGGCGTCCCTTCTTAGCTCTCGCCCGCCCCTTTCT
  GCAGCCTAGGCGGCCCGGGTTCTCTTCTCTTCCTCGCGCGCCCAGCCGCCTCGGTTCCCG
  GCGACCATGGTGACGATGGAGGAGCTGCGGGAGATGGACTGCAGTGTGCTCAAAAGGCTG
  ATGAACCGGGACGAGAATGGCGGCGGCGCGGGCGGCAGCGGCAGCCACGGCACCCTGGGG
  CTGCCGAGCGGCGGCAAGTGCCTGCTGCTGGACTGCAGACCGTTCCTGGCGCACAGCGCG
  GGCTACATCCTAGGTTCGGTCAACGTGCGCTGTAACACCATCGTGCGGCGGCGGGCTAAG
  GGCTCCGTGAGCCTGGAGCAGATCCTGCCCGCCGAGGAGGAGGTACGCGCCCGCTTGCGC
  TCCGGCCTCTACTCGGCGGTCATCGTCTACGACGAGCGCAGCCCGCGCGCCGAGAGCCTC
  CGCGAGGACAGCACCGTGTCGCTGGTGGTGCAGGCGCTGCGCCGCAACGCCGAGCGCACC
  GACATCTGCCTGCTCAAAGGCGGCTATGAGAGGTTTTCCTCCGAGTACCCAGAATTCTGT
  TCTAAAACCAAGGCCCTGGCAGCCATCCCACCCCCGGTTCCCCCCAGTGCCACAGAGCCC
  TTGGACCTGGGCTGCAGCTCCTGTGGGACCCCACTACACGACCAGGGGGGTCCTGTGGAG
  ATCCTTCCCTTCCTCTACCTCGGCAGTGCCTACCATGCTGCCCGGAGAGACATGCTGGAC
  GCCCTGGGCATCACGGCTCTGTTGAATGTCTCCTCGGACTGCCCAAACCACTTTGAAGGA
  CACTATCAGTACAAGTGCATCCCAGTGGAAGATAACCACAAGGCCGACATCAGCTCCTGG
  TTCATGGAAGCCATAGAGTACATCGATGCCGTGAAGGACTGCCGTGGGCGCGTGCTGGTG
  CACTGCCAGGCGGGCATCTCGCGGTCGGCCACCATCTGCCTGGCCTACCTGATGATGAAG
  AAACGGGTGAGGCTGGAGGAGGCCTTCGAGTTCGTTAAGCAGCGCCGCAGCATCATCTCG
  CCCAACTTCAGCTTCATGGGGCAGCTGCTGCAGTTCGAGTCCCAGGTGCTGGCCACGTCC
  TGTGCTGCGGAGGCTGCTAGCCCCTCGGGACCCCTGCGGGAGCGGGGCAAGACCCCCGCC
  ACCCCCACCTCGCAGTTCGTCTTCAGCTTTCCGGTCTCCGTGGGCGTGCACTCGGCCCCC
  AGCAGCCTGCCCTACCTGCACAGCCCCATCACCACCTCTCCCAGCTGTTAGAGCCGCCCT
  GGGGGCCCCAGAACCAGAGCTGGCTCCCAGCAAGGGTAGGACGGGCCGCATGCGGGCAGA
  AAGTTGGGACTGAGCAGCTGGGAGCAGGCGACCGAGCTCCTTCCCCATCATTTCTCCTTG
  GCCAACGACGAGGCCAGCCAGAATGGCAATAAGGACTCCGAATACATAATAAAAGCAAAC
  AGAACACTCCAACTTAGAGCAATAACGGCTGCCGCAGCAGCCAGGGAAGACCTTGGTTTG
  GTTTATGTGTCAGTTTCACTTTTCCGATAGAAATTTCTTACCTCATTTTTTTAAGCAGTA
  AGGCTTGAAGTGATGAAACCCACAGATCCTAGCAAATGTGCCCAACCAGCTTTACTAAAG
  GGGGAGGAAGGGAGGGCAAAGGGATGAGAAGACAAGTTTCCCAGAAGTGCCTGGTTCTGT
  GTACTTGTCCCTTTGTTGTCGTTGTCGTAGTTAAAGGAATTTCATTTTTTAAAAGAAAAA
  AAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001394 unedited
  CGGCAAAAACCTTCCGCACGAGGAGGAAGGGGACAGGNGAAGAAGAGGCTCTCCCGCGGG
  AGCCCTTGAGGACCAAGTTTGCGGCCACTTCTGCAGGCGTCCCTTCTTAGCTCTCGCCCG
  CCCCTTTCTGCAGCCTATGCGGCCCGGGTTCTCTTCTCTTCCTCGCGCGCCCAGCCGCCT
  CGGTGCCTGGCGACCATGGTGACGATGGATGAGCTGCGGGAGATGGACTGCAGTGTGCTC
  AAAAGGCTGATGAACCGGGACGAGAATGGCGGCGGCGCGGGCGGCAGCGGCAGCCACGGC
  ACCCTGGGGCTGCCGAGCGGCGGCAAGTGCCTGCTGCTGGACTGCAGACCGTTCCTGGCG
  CACAGCGCGGGCTACATCCTAGGTTCGGTCAACGTGCGCTGTAACACCATCGTGCGGCGG
  CGGGCTAAGGGCTCCGTGAGCCTGGAGCAGATCCTGCCCGCCGAGGAGGAGGTACGCGCC
  CGCTTGCGCTCCGGCCTCTACTCGGCGGTCATCGTCTACGACGAGCGCAGCCCGCGCGCC
  GAGAGCCTCCGCGAGGACAGCACCGTGTCGCTGGTGGTGCAAGCGCTGCGCCGCATCGCC
  GAGCGCACCGACATCTGCCTGCTCAAAGGCGGCTATGAGAGGTTTTCCTCCGAGTACCCA
  GAATTCTGTGTCTAAACCAAGGCCCTGGCAGCCATCCCACCCCNCGGGTTCCCCCAGTGC
  CACAGAGCCCTTGGACCTTGGCTGCAGCTCCTGTGGGACCCCACTACACGACCAGGGGAG
  GTCCCTGTGGAGAATCCTCCCTTCCTCTACCTCGGCAGTGCCTACCATGCCTGCCGGAGA
  GACTGCTGGACCCCTGGGCATACAGCTCTGTGGATGTCTCCTCGCCTGCCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001394 unedited
  CGCGGCCGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTCTTTTAAAAAATGAAATTC
  CTTTAACTACGACAACGACAACAAAGGGACAAGTACACAGAACCAGGCACTTCTGGGAAA
  CTTGTCTTCTCATCCCTTTGCCCTCCCTTCCTCCCCCTTTAGTAAAGCTGGTTGGGCACA
  TTTGCTAGGATCTGTGGGTTTCATCACTTCAAGCCTTACTGCTTAAAAAAATGAGGTAAG
  AAATTTCTATCGGAAAAGTGAAACTGACACATAAACCAAACCAAGGTCTTCCCTGGCTGC
  TGCGGCAGCCGTTATTGCTCTAAGTTGGAGTGTTCTGTTTGCTTTTATTATGTATTCGGA
  GTCCTTATTGCCATTCTGGCTGGCCTCGTCGTTGGCCAAGGAGAAATGATGGGGAAGGAG
  CTCGGTCGCCTGCTCCCAGCTGCTCAGTCCCAACTTTCTGCCCGCATGCGGCCCGTCCTA
  CCCTTGCTGGGAGCCAGCTCTGGTTCTGGGGCCCCCAGGGCGGCTCTAACAGCTGGGAGA
  GGCGGTGATGGGGCTGCGCAGGTAGGGCAGGCTGCTGGGGGCCGAGTGCCCGCCCACGGA
  GACCGGATAGCTGAAGACCAACTGCGAGGTGGGGGTGGCGGGGGCCTTGCCCCGCTCCCC
  AGGGGTCCCGAGGGGCTAGCAGACCTCGCAGCACAGGACGTGGCCAGCACCTGGGACTCG
  AACTGCAGCAGTTGCCCCAGAAGCTGAAGTTGGGCGAAATGATGCTGCGGCGCTGTTAAC
  CAACTCGAAGCCACCTCAACCTCACCCGTTTCTCATTATCAGTAGCCAAGCAAATGGGGC
  CGACGCGATAGCCCGGCTGGCATGCACCACCACGCCCCCCGGGAGACTTCCGGCACGAGG
  ACCCTATGGTCCAAGAACAAGAACGAAGNCGGCCTGAGGTATTCTCCCTGGGAGGCCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001394
• Insert Size: 1850 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001394.5, NP_001385.1
• RefSeq Size: 2498 bp
• RefSeq ORF: 1185 bp
• Locus ID: 1846
• UniProt ID: Q13115
• Cytogenetics: 8p12
• Domains: DSPc, RHOD, PTPc_motif
• Protein Families: Phosphatase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.