GDI2 (NM_001494) Human Untagged Clone

CAT#: SC119204

GDI2 (untagged)-Human GDP dissociation inhibitor 2 (GDI2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GDI2
• Synonyms: HEL-S-46e; RABGDIB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001494, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATGAGGAGTACGACGTGATCGTGCTGGGCACCGGCCTGACGGAATGTATCCTGTCAGGTATAATGT
  CAGTGAATGGCAAGAAAGTTCTTCATATGGATCGAAACCCTTACTACGGAGGAGAGAGTGCATCTATAAC
  ACCATTGGAAGATTTATACAAAAGATTTAAAATACCAGGATCACCACCCGAGTCAATGGGGAGAGGAAGA
  GACTGGAATGTTGACTTGATTCCCAAGTTCCTTATGGCTAATGGTCAGCTGGTTAAGATGCTGCTTTATA
  CAGAGGTAACTCGCTATCTGGATTTTAAAGTGACTGAAGGGAGCTTTGTCTATAAGGGTGGAAAAATCTA
  CAAGGTTCCTTCCACTGAAGCAGAAGCCCTGGCATCTAGCCTAATGGGATTGTTTGAAAAACGTCGCTTC
  AGGAAATTCCTAGTGTATGTTGCCAACTTCGATGAAAAAGATCCAAGAACTTTTGAAGGCATTGATCCTA
  AGAAGACCACAATGCGAGATGTGTATAAGAAATTTGATTTGGGTCAAGACGTTATAGATTTTACTGGTCA
  TGCTCTTGCACTTTACAGAACTGATGATTACTTAGATCAACCGTGTTATGAAACCATTAATAGAATTAAA
  CTTTACAGTGAATCTTTGGCAAGATATGGCAAAAGCCCATACCTTTATCCACTCTATGGCCTTGGAGAAC
  TGCCCCAAGGATTTGCAAGGCTAAGTGCTATTTATGGAGGTACCTATATGCTGAATAAACCCATTGAAGA
  AATCATTGTACAGAATGGAAAAGTAATTGGTGTAAAATCTGAAGGAGAAATTGCTCGCTGTAAGCAGCTC
  ATCTGTGACCCCAGCTACGTAAAAGATCGGGTAGAAAAAGTGGGCCAGGTGATCAGAGTTATTTGCATCC
  TCAGCCACCCCATCAAGAACACCAATGATGCCAACTCCTGCCAGATCATTATTCCACAGAACCAAGTCAA
  TCGAAAGTCAGATATCTACGTCTGCATGATCTCCTTTGCGCACAATGTAGCAGCACAAGGGAAGTACATT
  GCTATAGTTAGTACAACTGTGGAAACCAAGGAGCCTGAGAAGGAAATCAGACCAGCTTTGGAGCTCTTGG
  AACCAATTGAACAGAAATTTGTTAGCATCAGTGACCTCCTGGTACCAAAAGACTTGGGAACAGAAAGCCA
  GATCTTTATTTCCCGCACATATGATGCCACCACTCATTTTGAGACAACGTGTGATGACATTAAAAACATC
  TATAAGAGGATGACAGGATCAGAGTTTGACTTTGAGGAAATGAAGCGCAAGAAGAATGACATCTATGGGG
  AAGACTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001494 unedited
  GGTCAACATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCGCGCCCAA
  NAGCGCCTCCCAGCCTCGTAGGGTGGTCACGGAGCCCCTGCGCCTTTTCCTTGCTCGGGT
  CCTGCGTCCGCGCCTGCCCCGCCATGAATGAGGAGTACGACGTGATCGTGCTGGGCACCG
  GCCTGACGGAATGTATCCTGTCAGGTATAATGTCAGTGAATGGCAAGAAAGTTCTTCATA
  TGGATCGAAACCCTTACTACGGAGGAGAGAGTGCATCTATAACACCATTGGAAGATTTAT
  ACAAAAGATTTAAAATACCAGGATCACCACCCGAGTCAATGGGGAGAGGAAGAGACTGGA
  ATGTTGACTTGATTCCCAAGTTCCTTATGGCTAATGGTCAGCTGGTTAAGATGCTGCTTT
  ATACAGAGGTAACTCGCTATCTGGATTTTAAAGTGACTGAAGGGAGCTTTGTCTATAAGG
  GTGGAAAAATCTACAAGGTTCCTTCCACTGAAGCAGAAGCCCTGGCATCTAGCCTAATGG
  GATTGTTTGAAAAACGTCGCTTCAGGAAATTCCTAGTGTATGTTGCCAACTTCGATGAAA
  AAGATCCAAGAACTTTTGAAGGCATTGATCCTAAGAAGACCACAATGCGAGATGTGTATA
  AGAAATTTGATTTGGGTCAAGACGTTATAGATTTTACTGGTCATGCTCTTGCACTNTACA
  GAACTGATGATTACTTAGATCAACCGTGTTATGAAACCATTAATAGAATTAAACTTTACA
  GTGAATCTTTGGGCAGATATGGCANAAGCCCATACCTTTATCCACTCTATGGCCTTGGAG
  AACTGCCCCAGGATNTGCAGGNNCTAGTGCTATTTATGGAAGGTACCCTATATGCTGATA
  AACCCATTNGAAANTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001494 unedited
  TTGGACGCGGCCGCAATCTAGGATCGAGTTTTTNTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTCCAAAATTTGGCTTTATTTTGCAGTACAAAAATCATTTGGAGCCTTTTTGAAAC
  AAAAGTAAAGGCTCTGTCAAGTCAATACTGCATTGCAGCTTGGCCCACTGAAAAACCCAC
  CCCTGAAATACAAAAGATGCACTACACTTGACCCGCTTTATGTTCGTTTCCTCTCCCCTT
  TTTTTTAATAAACTTTATTAGGTTAAAACACCACATACAGGCTTTCTCCAAATGACTCCC
  TATGTCTGGGGTTGGGTTAAAATTTTATGCCCACATAAACCAAACTTGGGGTTATGCTAT
  TTGGGCCCTGCCATAACTTTTGACTTATTACAAAATATAAGGTCTTAGGGAAAACTTCCG
  GATGCTGTCCCAAACCGGGGAATATTCAATTTAAAATCTCCTGAACCTCAAGAGGACAGA
  TGACACACAACCTGTATGGATCCAGCTCTTGAGCAGCAGCAGCACATAGCTACACTGATT
  AAAAGATTAAAAAAACTGTCTCAAGTTGTCTGTGTCGGTATCCCAAGGTTTGTTTAACTT
  CACTAGAAAAAAAAAAAAAGCCAGTTTGGTTAAATTGAACAGAAGGTGGAAACTGCCAAT
  GCTGAATACCCACTCCATGAAACAAGTTAATAATTAGAAGGTGAAGGGGAGTATTACTGG
  ACAGGGCTCTTATTCTCTCATTTCATTACAAAGCAGCCCTACAATATGATTCATTATAGC
  CATATTGCCAAATTTTAAAGTGNCCTAATACATATAACATGAACTGCTGTTAGCCTCCCC
  AAGATGCATCCTTCTGCGCTAATTTCTAAAGCCAACTCTGACCTGCATCCTTTAAAGAGG
  TNTAATGGCTNCCACGTGCCTCAAAGGAGGGGGGCATATATGCGCGGAAATAAGATCTGC
  TT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001494
• Insert Size: 2380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001494.2, NP_001485.2
• RefSeq Size: 2274 bp
• RefSeq ORF: 1338 bp
• Locus ID: 2665
• UniProt ID: P50395
• Cytogenetics: 10p15.1
• Domains: GDI
• Gene Summary: GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).