IGFBP7 (NM_001553) Human Untagged Clone

CAT#: SC119176

IGFBP7 (untagged)-Human insulin-like growth factor binding protein 7 (IGFBP7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IGFBP7
• Synonyms: AGM; FSTL2; IBP-7; IGFBP-7; IGFBP-7v; IGFBPRP1; MAC25; PSF; RAMSVPS; TAF
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001553 edited
  GAATTCGGCACGAGGCCGCTGCCACCGCACCCCGCCATGGAGCGGCCGTCGCTGCGCGCC
  CTGCTCCTCGGCGCCGCTGGGCTGCTGCTCCTGCTCCTGCCCCTCTCCTCTTCCTCCTCT
  TCGGACACCTGCGGCCCCTGCGAGCCGGCCTCCTGCCCGCCCCTGCCCCCGCTGGGCTGC
  CTGCTGGGCGAGACCCGCGACGCGTGCGGCTGCTGCCCTATGTGCGCCCGCGGCGAGGGC
  GAGCCGTGCGGGGGTGGCGGCGCCGGCAGGGGGTACTGCGCGCCGGGCATGGAGTGCGTG
  AAGAGCCGCAAGAGGCGGAAGGGTAAAGCCGGGGCAGCAGCCGGCGGTCCGGGTGTAAGC
  GGCGTGTGCGTGTGCAAGAGCCGCTACCCGGTGTGCGGCAGCGACGGCACCACCTACCCG
  AGCGGCTGCCAGCTGCGCGCCGCCAGCCAGAGGGCCGAGAGCCGCGGGGAGAAGGCCATC
  ACCCAGGTCAGCAAGGGCACCTGCGAGCAAGGTCCTTCCATAGTGACGCCCCCCAAGGAC
  ATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAGGTCATCGGAATCCCGACA
  CCTGTCCTCATCTGGAACAAGGTAAAAAGGGGTCACTATGGAGTTCAAAGGACAGAACTC
  CTGCCTGGTGACCGGGACAACCTGGCCATTCAGACCCGGGGTGGCCCAGAAAAGCATGAA
  GTAACTGGCTGGGTGCTGGTATCTCCTCTAAGTAAGGAAGATGCTGGAGAATATGAGTGC
  CATGCATCCAATTCCCAAGGACAGGCTTCAGCATCAGCAAAAATTACAGTGGTTGATGCC
  TTACATGAAATACCAGTGAAAAAAGGTGAAGGTGCCGAGCTATAAACCTCCAGAATATTA
  TTAGTCTGCATGGTTAAAAGTAGTCATGGATAACTACATTACCTGTTCTTGCCTAATAAG
  TTTCTTTTAATCCAATCCACTAACACTTTAGTTATATTCACTGGTTTTACACAGAGAAAT
  ACAAAATAAAGATCACACATCAAGACTATCTACAAAAATTTATTATATATTTACAGAAGA
  AAAGCATGCATATCATTAAACAAATAAAATACTTTTTATCACAACACAAAAAAAAAAAAA
  AAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001553 unedited
  NGGGTTTTAGGATTTGTNATCCGACTTCACTATNAGGNNCGGCCGCGCAATTCGGCACGA
  GGCCGCTGCCACCGCACCCCGCCATGGAGCGGCCGTCGCTGCGCGCCCTGCTCCTCGGCG
  CCGCTGGGCTGCTGCTCCTGCTCCTGCCCCTCTCCTCTTCCTCCTCTTCGGACACCTGCG
  GCCCCTGCGAGCCGGCCTCCTGCCCGCCCCTGCCCCCGCTGGGCTGCCTGCTGGGCGAGA
  CCCGCGACGCGTGCGGCTGCTGCCCTATGTGCGCCCGCGGCGAGGGCGAGCCGTGCGGGG
  GTGGCGGCGCCGGCAGGGGGTACTGCGCGCCGGGCATGGAGTGCGTGAAGAGCCGCAAGA
  GGCGGAAGGGTAAAGCCGGGGCAGCAGCCGGCGGTCCGGGTGTAAGCGGCGTGTGCGTGT
  GCAAGAGCCGCTACCCGGTGTGCGGCAGCGACGGCACCACCTACCCGAGCGGCTGCCAGC
  TGCGCGCCGCCAGCCAGAGGGCCGAGAGCCGCGGGGAGAAGGCCATCACCCAGGTCAGCA
  AGGGCACCTGCGAGCAAGGTCCTTCCATAGTGACGCCCCCCAAGGACATCTGGAATGTCA
  CTGGTGCCCAGGTGTACTTGAGCTGTGAGGTCATCGGAATCCCGACACCTGTCCTCATCT
  GGAACAAGGTAAAAAGGGGTCACTATGGAGTTCAAAGGACAGAACTCCTGCCTGGTGACC
  GGGACAACCCTGCCATTCAGACCCGGGGTGGCCCCAGAAAGCATGAAGTAACTGGCTGGG
  TGCTGGTATCTCCTCTAAGTAGGAAGATGCTGNAGAATATGAGTGCCATGCATCCAATTN
  CCAAGGACAGGCTTCAGCATCAGCAAAAATACAGTGGGTGATGCCCTACATGAATAACAG
  TGAAAAAAGTGAAAGTGCCCGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001553 unedited
  TTTAACGGCCTCTATGTACCGCGGCCGCTTTCNAAGATCGGTTTTTTCCTTTTTTTTTTT
  TTTGTGTTGTGATAAAAAGTTTTTATTTGTTTAATGATATGCATGCTTTTCTTCTGTAAA
  TATATAATAAATTTTTGTAGATAGTCTTGATGTGTGATCTTTATTTTGTATTTCTCTGTG
  TAAAACCAGTGAATATAACTAAAGTGTTAGTGGATTGGATTAAAAGAAACTTATTAGGCA
  AGAACAGGTAATGTAGTTATCCATGACTACTTTTAACCATGCAGACTAATAATATTCTGG
  AGGTTTATAGCTCGGCACCTTCACCTTTTTTCACTGGTATTTCATGTAAGGCATCAACCA
  CTGTAATTTTTGCTGATGCTGAAGCCTGTCCTTGGGAATTGGATGCATGGCACTCATATT
  CTCCAGCATCTTCCTTACTTAGAGGAGATACCAGCACCCAGCCAGTTACTTCATGCTTTT
  CTGGGCCACCCCGGGTCTGAATGGCCAGGTTGTCCCGGTCACCAAGCAGGAGTTCTGTCC
  TTTGAACTCCATAGTGACCCCTTTTTACCTTGTTCCAGATGAAGACAGGTGTCGGGATTC
  CGATGACCTCACAGCTCAAGTACACCTGGGCACCAGTGACATTCCAGATGTCCTTGGGGG
  GCGTCACTATGGAAAGACCTTGCTCGCAAGTGCCCTTGCTGACCTGGGTGATGGCCTTCT
  CCCCGCGGCTCTCGGCCCTCTGGCTGGCGGCGCGCATCTGGCAGCCGCTCGGTAAGTGGG
  TGGCCCGTCGCTGCCGCACACCGGGGTAGCGGNCTCTTGCACACGCACACGCCGCTTACA
  CCCTNACCGCCGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001553
• Insert Size: 1200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001553.1, NP_001544.1
• RefSeq Size: 1124 bp
• RefSeq ORF: 849 bp
• Locus ID: 3490
• UniProt ID: Q16270
• Cytogenetics: 4q12
• Domains: IB, kazal, IG
• Protein Families: Secreted Protein
• Gene Summary: This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm (PMID:21835307). [provided by RefSeq, Dec 2011]
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).