gamma Actin (ACTG1) (NM_001614) Human Untagged Clone

CAT#: SC119154

ACTG1 (untagged)-Human actin, gamma 1 (ACTG1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: gamma Actin
• Synonyms: ACT; ACTG; DFNA20; DFNA26; HEL-176
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119154 sequence for NM_001614 edited (data generated by NextGen Sequencing)
  ATGGAAGAAGAGATCGCCGCGCTGGTCATTGACAATGGCTCCGGCATGTGCAAAGCTGGT
  TTTGCTGGGGACGACGCTCCCCGAGCCGTGTTTCCTTCCATCGTCGGGCGCCCCAGACAC
  CAGGGCGTCATGGTGGGCATGGGCCAGAAGGACTCCTACGTGGGCGACGAGGCCCAGAGC
  AAGCGTGGCATCCTGACCCTGAAGTACCCCATTGAGCATGGCATCGTCACCAACTGGGAC
  GACATGGAGAAGATCTGGCACCACACCTTCTACAACGAGCTGCGCGTGGCCCCGGAGGAG
  CACCCAGTGCTGCTGACCGAGGCCCCCCTGAACCCCAAGGCCAACAGAGAGAAGATGACT
  CAGATTATGTTTGAGACCTTCAACACCCCGGCCATGTACGTGGCCATCCAGGCCGTGCTG
  TCCCTCTACGCCTCTGGGCGCACCACTGGCATTGTCATGGACTCTGGAGACGGGGTCACC
  CACACGGTGCCCATCTACGAGGGCTACGCCCTCCCCCACGCCATCCTGCGTCTGGACCTG
  GCTGGCCGGGACCTGACYGACTACCTCATGAAGATCCTCACTGAGCGAGGCTACAGCTTC
  ACCACCACGGCCGAGCGGGAAATCGTGCGYGACATYAAGGAGAAGCTGTGCTACGTCGCC
  CTGGACTTCGAGCAGGAGATGGCCACCGCCGCATCCTCCTCTTCTCTGGAGAAGAGCTAC
  GAGCTGCCYGAYGGCCAGGTCATCACCATTGGCAATGAGCGGTTCCGGTGTCCGGAGGCG
  CTGTTCCAGCCTTCCTTCCTGGGTATGGAATCTTGCGGCATCCACGAGACYACCTTCAAC
  TCCATCATGAAGTGTGACGTGGACATCCGCAAAGACCTGTACGCCAACACRGTGCTGTCG
  GGCGGCACCACCATGTATCCGGGCATTGCCGACAGGATGCAGAAGGAGATCACCGCCCTG
  GCGCCCAGCACCATGAAGATCAAGATCATCGCACCCCCAGAGCGCAAGTACTCSGTGTGG
  ATCGGYGGCTCCATCCTGGCCTCRCTGTCCACCTTCCAGCAGATGTGGATYAGCAAGCAG
  GAGTACGACGAGTCGGGCCCCTCCATCGTCCACCGCAAATGCTTCTAA
  
  Clone variation with respect to NM_001614.3
  558 c=>y;630 c=>y;636 c=>y;729 c=>y;732 t=>y;831 c=>y;891 g=>r;918 c=>t;1014 g=>s;1026 t=>y;1044 a=>r;1071 t=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001614 unedited
  CATATATTTGTATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGCCAACTCT
  CGCACTCTGTTCTTCCGCCGCTCCGCCGTCGCGTTTCTCTGCCGGTCGCAATGGAAGAAG
  AGATCGCCGCGCTGGTCATTGACAATGGCTCCGGCATGTGCAAAGCTGGTTTTGCTGGGG
  ACGACGCTCCCCGAGCCGTGTTTCCTTCCATCGTCGGGCGCCCCAGACACCAGGGCGTCA
  TGGTGGGCATGGGCCAGAAGGACTCCTACGTGGGCGACGAGGCCCAGAGCAAGCGTGGCA
  TCCTGACCCTGAAGTACCCCATTGAGCATGGCATCGTCACCAACTGGGACGACATGGAGA
  AGATCTGGCACCACACCTTCTACAACGAGCTGCGCGTGGCCCCGGAGGAGCACCCAGTGC
  TGCTGACCGAGGCCCCCCTGAACCCCAAGGCCAACAGAGAGAAGATGACTCAGATTATGT
  TTGAGACCTTCAACACCCCGGCCATGTACGTGGCCATCCAGGCCGTGCTGTCCCTCTACG
  CCTCTGGGCGCACCACTGGCATTGTCATGGACTCTGGAGACGGNGTCACCCACACGGTGC
  CCATCTACGAAGGCTACGCCCTCCCCCACGCCATCCTGCGTCTGGACCTGGCTGGCCGGG
  ACCTGACCGACTACCTCATGAAGATCCTCACTGAGCGAGGCTACAGCTTCACCACCACGG
  CCGAGCGGGAAATCGTGCGCGACATCAAAGGAGAAGCTGTGCTACGTCGCCCCTGGACTT
  CGAGCANGAGATGGCCACCGNCGCATNCTCCTCTTCTCTGGAGAAGAGCTACCAGCTGNC
  CCGATGCCCCAGTCATCACCATTGGNCATGAGCGGGTTCCCGTGTTCCGGAGGCCCTGTT
  CCAGCCTTCCTTCTTGGGTATGGAATTTTGCGGCATTCACGAGAACACCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001614 unedited
  GCCGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGGTTACGGCAGCACTTTTATTTT
  TCCTTACACAATGACGTGTTGCTGGGGCCTAATGTTCTCACATAACAGTAGAAAACCAAA
  ATTTGTTGTCATCTCTTCAAAGAATCGAGAATTGCGTACAAAAAAAACCTTACATAAATT
  AAGAATGAATACATTTACAGGCGTAAATGCAAACCGCTTCCAACTCAAAGCAAGTAACAG
  CCCACGGTGTTCTGGCCAAAGACATCAGCTAAGAAAGGAAACTGGGTCCTACGGCTTGGA
  CTTTCCAACCCTGACAGACCCGCAAGACAAAACAACTGGTTCTTGCCAGCCTCTAGAGAA
  ATCCCAGAACACTCAGCCCTGACACGTTAATACCCTGCACAGATCAGAGGCTGCTGGCCA
  CACAGACTCACCAAGCCACAGACTTGTCTTCCACAAGCACGTTCTTACCTTATCCACGAA
  GTGACCAAGCCACACGTACTAAAGGTTGAACTCAAAGATATGTACAGGGTATTAAACAAA
  TACCAAGGGGAACAGTTAACTTCAATACAAGGTCAAAATCAGCAACAAGTTCTACAATCC
  AGTGCTGATATCAGATACAAGCTTCAAGGACAATTTCTTTTCGAAGGCTTATTCCAGTTT
  CGTGAGGCTAGCATGAAGTGTGTGCATTTGCCAGGGGCAAATTTCTATTTCTCAATTAAC
  CCATGCAGCAAATGCTACGCATCTGCTGAGTCCGTTTAAAGCACTTGCGGTCGACGATTG
  GAGGGCCCGACTCCGCGTACTCCTGCTTGCTATTCCCCATCTGCCTGGAAGTTCACAGTG
  AGGCCAGGATTGAGCCACCGATCCCCACCGAGTCTTGCGCTCTGGGGCTGCAAGATCTTG
  ATCTCATGGGGCTGGCGCCAGGCCGGGATTTCTTTTGTTCCTGCGGCAATGCCGGTACAT
  GTTGGGTCCCCGCAAACCTGTTGGCGCCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001614
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001614.2, NP_001605.1
• RefSeq Size: 1919 bp
• RefSeq ORF: 1128 bp
• Locus ID: 71
• UniProt ID: P63261
• Cytogenetics: 17q25.3
• Domains: ACTIN
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis
• Gene Summary: Actins are highly conserved proteins that are involved in various types of cell motility and in maintenance of the cytoskeleton. Three main groups of actin isoforms have been identified in vertebrate animals: alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Actin gamma 1, encoded by this gene, is a cytoplasmic actin found in all cell types. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss and also with Baraitser-Winter syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.