HIF1 beta (ARNT) (NM_001668) Human Untagged Clone

CAT#: SC119100

ARNT (untagged)-Human aryl hydrocarbon receptor nuclear translocator (ARNT), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HIF1 beta
• Synonyms: bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001668 edited
  GGCGGTCAGGCGCCGCTTCTGGGGAGTGGCCTTTCTTTTCCCCTCCCTCCCGGTTCGGTG
  GCGGCGGCTCCTCCCACTGGGGGGGGGTGGCGCGGCGGCGGTGGCATCTGCGGCCATGGC
  GGCGACTACTGCCAACCCCGAAATGACATCAGATGTACCATCACTGGGTCCAGCCATTGC
  CTCTGGAAACTCTGGACCTGGAATTCAAGGTGGAGGAGCCATTGTCCAGAGGGCTATTAA
  GCGGCGACCAGGGCTGGATTTTGATGATGATGGAGAAGGGAACAGTAAATTTTTGAGGTG
  TGATGATGATCAGATGTCTAACGATAAGGAGCGGTTTGCCAGGTCGGATGATGAGCAGAG
  CTCTGCGGATAAAGAGAGACTTGCCAGGGAAAATCACAGTGAAATTGAACGGCGGCGACG
  GAACAAGATGACAGCCTACATCACAGAACTGTCAGATATGGTACCCACCTGTAGTGCCCT
  GGCTCGAAAACCAGACAAGCTAACCATCTTACGCATGGCAGTTTCTCACATGAAGTCCTT
  GCGGGGAACTGGCAACACATCCACTGATGGCTCCTATAAGCCGTCTTTCCTCACTGATCA
  GGAACTGAAACATTTGATCTTGGAGGCAGCAGATGGCTTTCTGTTTATTGTCTCATGTGA
  GACAGGCAGGGTGGTGTATGTGTCTGACTCCGTGACTCCTGTTTTGAACCAGCCACAGTC
  TGAATGGTTTGGCAGCACACTCTATGATCAGGTGCACCCAGATGATGTGGATAAACTTCG
  TGAGCAGCTTTCCACTTCAGAAAATGCCCTGACAGGGCGTATCCTGGATCTAAAGACTGG
  AACAGTGAAAAAGGAAGGTCAGCAGTCTTCCATGAGAATGTGTATGGGCTCAAGGAGATC
  GTTTATTTGCCGAATGAGGTGTGGCAGTAGCTCTGTGGACCCAGTTTCTGTGAATAGGCT
  GAGCTTTGTGAGGAACAGATGCAGGAATGGACTTGGCTCTGTAAAGGATGGGGAACCTCA
  CTTCGTGGTGGTCCACTGCACAGGCTACATCAAGGCCTGGCCCCCAGCAGGTGTTTCCCT
  CCCAGATGATGACCCAGAGGCTGGCCAGGGAAGCAAGTTTTGCCTAGTGGCCATTGGCAG
  ATTGCAGGTAACTAGTTCTCCCAACTGTACAGACATGAGTAATGTTTGTCAACCAACAGA
  GTTCATCTCCCGACACAACATTGAGGGTATCTTCACTTTTGTGGATCACCGCTGTGTGGC
  TACTGTTGGCTACCAGCCACAGGAACTCTTAGGAAAGAATATTGTAGAATTCTGTCATCC
  TGAAGACCAGCAGCTTCTAAGAGACAGCTTCCAACAGGTAGTGAAATTAAAAGGCCAAGT
  GCTGTCTGTCATGTTCCGGTTCCGGTCTAAGAACCAAGAATGGCTCTGGATGAGAACCAG
  CTCCTTTACTTTCCAGAACCCTTACTCAGATGAAATTGAGTACATCATCTGTACCAACAC
  CAATGTGAAGAACTCTAGCCAAGAACCACGGCCTACACTCTCCAACACAATCCAGAGGCC
  ACAACTAGGTCCCACAGCTAATTTACCCCTGGAGATGGGCTCAGGACAGCTGGCACCCAG
  GCAGCAGCAACAGCAAACAGAATTGGACATGGTACCAGGAAGAGATGGACTGGCCAGCTA
  CAATCATTCCCAGGTGGTTCAGCCTGTGACAACCACAGGACCAGAACACAGCAAGCCCCT
  TGAGAAGTCAGATGGTTTATTTGCCCAGGATAGAGATCCAAGATTTTCAGAAATCTATCA
  CAACATCAATGCGGATCAGAGTAAAGGCATCTCCTCCAGCACTGTCCCTGCCACCCAACA
  GCTATTCTCCCAGGGCAACACATTCCCTCCTACCCCCCGGCCGGCAGAGAATTTCAGGAA
  TAGTGGCCTAGCCCCTCCTGTAACCATTGTCCAGCCATCAGCTTCTGCAGGACAGATGTT
  GGCCCAGATTTCCCGCCACTCCAACCCCACCCAAGGAGCAACCCCAACTTGGACCCCTAC
  TACCCGCTCAGGCTTTTCTGCCCAGCAGGTGGCTACCCAGGCTACTGCTAAGACTCGTAC
  TTCCCAGTTTGGTGTGGGCAGCTTTCAGACTCCATCCTCCTTCAGCTCCATGTCCCTCCC
  TGGTGCCCCAACTGCATCGCCTGGTGCTGCTGCCTACCCTAGTCTCACCAATCGTGGATC
  TAACTTTGCTCCTGAGACTGGACAGACTGCAGGACAATTCCAGACACGGACAGCAGAGGG
  TGTGGGTGTCTGGCCACAGTGGCAGGGCCAGCAGCCTCATCATCGTTCAAGTTCTAGTGA
  GCAACATGTTCAACAACCGCCAGCACAGCAACCTGGCCAGCCTGAGGTCTTCCAGGAGAT
  GCTGTCCATGCTGGGAGATCAGAGCAACAGCTACAACAATGAAGAATTCCCTGATCTAAC
  TATGTTTCCCCCCTTTTCAGAATAGAACTATTGGGGTGAGGATAAGGGGTGGGGGAGAAA
  AAATCACTGTTTGTTTTTAAAAAGCAAATCTTTCTGTAAACAGAATAAAAGTTCCTCTCC
  CTTCCCTTCCCTCACCCCTGACATGTACCCCCTTTCCCTTCTGGCTGTTCCCCTGCTCTG
  TTGCCTCTCTAAGGTAACATTTATAGAAGAAATGGAATGAATCTCCAAGGCTTTTAGGAC
  TGTCTGAAAATTTGAGGCTGGGTGAAGTTAAAACACCTTTCCTTATGTCTCCTGACCTGA
  AATTGTATAGTGTTGATTTGTGCTGAGATCAAGAGGCAGGTTAGAAGAACCTGACATCCA
  CTGTTTGCCTTGGATAGTATGGCTTGTTTTTGGAAAGAAATTCTGAAGAGAGTGGAGGAG
  AGGAGAAATGTCCTCATATTTGAGGACCATGAAACATTGTAGGTATATATGGGGCTTTAG
  CAAGTTTGAGCATAGGCTCTTTTTGCTGCCTGTGAGCAGTCCCTCTGGAAAGAAACATGT
  GAGTAAGTGAGAGAGAGTGTGTGTGTATGTGTGTGTGTGTGTGTGTGTGTGTGCGCACAC
  ATGCTTCTGTATTTCACTCTTTCTCCCTATTAGGGAGTTATGCAAAATTTGTCCCCGATT
  TTACCTTTGTCTTTCTGTGTACTTTTCAAAGAGTCCTAAGGAGTTAAATCTTCCAGGTAT
  TTTCCACTTAGTATTGCAGCCAAAGAATATTTAAATAAACGTCTTTGCTGCGCTTGCAAA
  AAAAAAAAAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001668
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001668.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001668.2, NP_001659.1
• RefSeq Size: 4846 bp
• RefSeq ORF: 2370 bp
• Locus ID: 405
• UniProt ID: P27540
• Cytogenetics: 1q21.3
• Domains: PAS, HLH, PAC
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma
• Gene Summary: This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.