C1S (NM_001734) Human Untagged Clone

CAT#: SC119071

C1S (untagged)-Human complement component 1, s subcomponent (C1S), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: C1S
• Synonyms: EDSPD2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001734, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGTGCATTGTCCTGTTTTCACTTTTGGCATGGGTTTATGCTGAGCCTACCATGTATGGGGAGATCC
  TGTCCCCTAACTATCCTCAGGCATATCCCAGTGAGGTAGAGAAATCTTGGGACATAGAAGTTCCTGAAGG
  GTATGGGATTCACCTCTACTTCACCCATCTGGACATTGAGCTGTCAGAGAACTGTGCGTATGACTCAGTG
  CAGATAATCTCAGGAGACACTGAAGAAGGGAGGCTCTGTGGACAGAGGAGCAGTAACAATCCCCACTCTC
  CAATTGTGGAAGAGTTCCAAGTCCCATACAACAAACTCCAGGTGATCTTTAAGTCAGACTTTTCCAATGA
  AGAGCGTTTTACGGGGTTTGCTGCATACTATGTTGCCACAGACATAAATGAATGCACAGATTTTGTAGAT
  GTCCCTTGTAGCCACTTCTGCAACAATTTCATTGGTGGTTACTTCTGCTCCTGCCCCCCGGAATATTTCC
  TCCATGATGACATGAAGAATTGCGGAGTTAATTGCAGTGGGGATGTATTCACTGCACTGATTGGGGAGAT
  TGCAAGTCCCAATTATCCCAAACCATATCCAGAGAACTCAAGGTGTGAATACCAGATCCGGTTGGAGAAA
  GGGTTCCAAGTGGTGGTGACCTTGCGGAGAGAAGATTTTGATGTGGAAGCAGCTGACTCAGCGGGAAACT
  GCCTTGACAGTTTAGTTTTTGTTGCAGGAGATCGGCAATTTGGTCCTTACTGTGGTCATGGATTCCCTGG
  GCCTCTAAATATTGAAACCAAGAGTAATGCTCTTGATATCATCTTCCAAACTGATCTAACAGGGCAAAAA
  AAGGGCTGGAAACTTCGCTATCATGGAGATCCAATGCCCTGCCCTAAGGAAGACACTCCCAATTCTGTTT
  GGGAGCCTGCGAAGGCAAAATATGTCTTTAGAGATGTGGTGCAGATAACCTGTCTGGATGGGTTTGAAGT
  TGTGGAGGGACGTGTTGGTGCAACATCTTTCTATTCGACTTGTCAAAGCAATGGAAAGTGGAGTAATTCC
  AAACTGAAATGTCAACCTGTGGACTGTGGCATTCCTGAATCCATTGAGAATGGTAAAGTTGAAGACCCAG
  AGAGCACTTTGTTTGGTTCTGTCATCCGCTACACTTGTGAGGAGCCATATTACTACATGGAAAATGGAGG
  AGGTGGGGAGTATCACTGTGCTGGTAACGGGAGCTGGGTGAATGAGGTGCTGGGCCCGGAGCTGCCGAAA
  TGTGTTCCAGTCTGTGGAGTCCCCAGAGAACCCTTTGAAGAAAAACAGAGGATAATTGGAGGATCCGATG
  CAGATATTAAAAACTTCCCCTGGCAAGTCTTCTTTGACAACCCATGGGCTGGTGGAGCGCTCATTAATGA
  GTACTGGGTGCTGACGGCTGCTCATGTTGTGGAGGGAAACAGGGAGCCAACAATGTATGTTGGGTCCACC
  TCAGTGCAGACCTCACGGCTGGCAAAATCCAAGATGCTCACTCCTGAGCATGTGTTTATTCATCCGGGAT
  GGAAGCTGCTGGAAGTCCCAGAAGGACGAACCAATTTTGATAATGACATTGCACTGGTGCGGCTGAAAGA
  CCCAGTGAAAATGGGACCCACCGTCTCTCCCATCTGCCTACCAGGCACCTCTTCCGACTACAACCTCATG
  GATGGGGACCTGGGACTGATCTCAGGCTGGGGCCGAACAGAGAAGAGAGATCGTGCTGTTCGCCTCAAGG
  CGGCAAGGTTACCTGTAGCTCCTTTAAGAAAATGCAAAGAAGTGAAAGTGGAGAAACCCACAGCAGATGC
  AGAGGCCTATGTTTTCACTCCTAACATGATCTGTGCTGGAGGAGAGAAGGGCATGGATAGCTGTAAAGGG
  GACAGTGGTGGGGCCTTTGCTGTACAGGATCCCAATGACAAGACCAAATTCTACGCAGCTGGCCTGGTGT
  CCTGGGGGCCCCAGTGTGGGACCTATGGGCTCTACACACGGGTAAAGAACTATGTTGACTGGATAATGAA
  GACTATGCAGGAAAATAGCACCCCCCGTGAGGACTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001734 unedited
  TCAGTAATTTGTAATACGACTCACTATTAGGGCGGCCGCGNAATTCGCACCAGGCTGGCC
  GGAGGTTCCTGCAGAGGAGCGTCAAGGCCCTGTGCTGCTGTCCCTGGGGGCCAGAGGGGT
  TGCCCAGCATGCCCACTGGCAGGAGAGAGGGAACTGACCCACTTGCTCCTACCAGCTTCT
  GAAGGCTCCAAAGTCCGGAGGTGCAGAAAGCCAGGACCAAGATACAGGCAGCTCACCAGG
  GTGGACAAATCGCCAGAGATGTGGTGCATTGTCCTGTTTTCACTTTTGGCATGGGTTTAT
  GCTGAGCCTACCATGTATGGGGAGATCCTGTCCCCTAACTATCCTCAGGCATATCCCAGT
  GAGGTAGAGAAATCTTGGGACATAGAAGTTCCTGAAGGGTATGGGATTCACCTCTACTTC
  ACCCATCTGGACATTGAGCTGTCAGAGAACTGTGCGTATGACTCAGTGCAGATAATCTCA
  GGAGACACTGAAGAAGGGAGGCTCTGTGGACAGAGGAGCAGTAACAATCCCCACTCTCCA
  ATTGTGGAAGAGTTCCAAGTCCCATACAACAAACTCCAGGTGATCTTTAAGTCAGACTTT
  TCCAATGAAGAGCGTTTTACGGAGTTTGCTGCATACTATGTTGCCACAGACATAAATGAA
  TGCACAGATTCTGTAGATGTCCCTTGTAGCCACTTCTGCAACAATTTCATTGGTGGTTAC
  TTCTGCTCCTGCCCCCCGGAATATTTNCTCCATGATGACATGAAGAATTGCGGAGTTAAT
  TGCAGTGGGGATGTATTCACTGCACTGATTGGNGAGATTNGCAGTTCCATTATTCCAAAC
  CTATCCAGAGACTCAAGTGTGAATACAGATCCGTNTGGAGATAGGCTNCAGTGGGGGTGA
  CCTTGCGAGAGATATNTGATGTGGAGCACTGACTCACGGAAACTGCTGACAGTTATTTTT
  GTGCAGAGATCGCATTGGTCCTACGTGTCAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001734 unedited
  NGAGCCATTGGCGATGTGCAACTTCCCAGGTCCAGNAAAGCACTGGGGNAGGGTCACAGG
  GCTGCCACCCGGGTATCTGTTGCAGGAAAACAGCTATGACCGCGGCCGCAATCTAGAGTC
  GAGTTTTTTTTTTTTTTTTTTGCTTATAAACATCCTTTATTGTACATAGACAGTGGATAC
  TGAGAATGATCAAGTAAATGGAATTTTGAACAGGTAAAGAGGAAACAAAGAATTAAGGTA
  TCCCTGTGGAATAGTGCAAGAAAGGAGTGCCCCACCCATAGTGTTATCTACAATAGGTAC
  TCCGGGGAAAGGACCCCAAGGAGTCAGACCACAAATGTATGACCAGCACAATTCTATGAT
  CAAACTCTACCTCTAGCAAGGCGTCTCAACAATCAAGTTCTATTTAAATCATTCGCTCGT
  GTCTTCTTTCAGTCATGATGAAATAATGAGAATATCATAAGGAACAGAAGGTAATGCATT
  GGTCACCACCCTTGGAGAGGCTGGTGGGATGTATCTGGATTAGTCCTCACGGGGGGTGCT
  ATTTTCCTGCATAGTCTTCATTATCCAGTCAACATAGTTCTTTACCCGTGTGTAGAGCCC
  ATAGGTCCCACACTGGGGCCCCCAGGACACCAGGCCAGCTGCGTAGAATTTGGTCTTGTC
  ATTGGGATCCTGTACAGCAAAGGCCCCACCACTGTCCCCTTTACAGCTATCCATGCCCTT
  CTCTCCTCCAGCACAGATCATGTTAGGAGTGAAAACATAGGCCTCTGCATCTGCTGTGGG
  TTTCTCCACTTTCACTTCTTTGCATTTTCTTAAAGGAGCTACAGGTAACCTTGCCGCCTT
  TGAGCGAACAGCACGATCTCTCTTCTCTGTNTCGCCCCAGCCTGAGATCAGTCCCAGGTC
  CCCATCCATGATGTTGTANTCAGAAAGAGTGCCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001734
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001734.2, NP_001725.1
• RefSeq Size: 2714 bp
• RefSeq ORF: 2067 bp
• Locus ID: 716
• UniProt ID: P09871
• Cytogenetics: 12p13.31
• Domains: CCP, CUB, Tryp_SPc, EGF_CA, EGF
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Complement and coagulation cascades, Systemic lupus erythematosus
• Gene Summary: This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same isoform (1).