CCN2 (NM_001901) Human Untagged Clone

CAT#: SC118973

CTGF (untagged)-Human connective tissue growth factor (CTGF)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CCN2
• Synonyms: CTGF; HCS24; IGFBP8; NOV2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118973 sequence for NM_001901 edited (data generated by NextGen Sequencing)
  ATGACCGCCGCCAGTATGGGCCCCGTCCGCGTCGCCTTCGTGGTCCTCCTCGCCCTCTGC
  AGCCGGCCGGCCGTCGGCCAGAACTGCAGCGGGCCGTGCCGGTGCCCGGACGAGCCGGCG
  CCGCGCTGCCCGGCGGGCGTGAGCCTCGTGCTGGACGGCTGCGGCTGCTGCCGCGTCTGC
  GCCAAGCAGCTGGGCGAGCTGTGCACCGAGCGCGACCCCTGCGACCCGCACAAGGGCCTC
  TTCTGTGACTTCGGCTCCCCGGCCAACCGCAAGATCGGCGTGTGCACCGCCAAAGATGGT
  GCTCCCTGCATCTTCGGTGGTACGGTGTACCGCAGCGGAGAGTCCTTCCAGAGCAGCTGC
  AAGTACCAGTGCACGTGCCTGGACGGGGCGGTGGGCTGCATGCCCCTGTGCAGCATGGAC
  GTTCGTCTGCCCAGCCCTGACTGCCCCTTCCCGAGGAGGGTCAAGCTGCCCGGGAAATGC
  TGCGAGGAGTGGGTGTGTGACGAGCCCAAGGACCAAACCGTGGTTGGGCCTGCCCTCGCG
  GCTTACCGACTGGAAGACACGTTTGGCCCAGACCCAACTATGATTAGAGCCAACTGCCTG
  GTCCAGACCACAGAGTGGAGCGCCTGTTCCAAGACCTGTGGGATGGGCATCTCCACCCGG
  GTTACCAATGACAACGCCTCCTGCAGGCTAGAGAAGCAGAGCCGCCTGTGCATGGTCAGG
  CCTTGCGAAGCTGACCTGGAAGAGAACATTAAGAAGGGCAAAAAGTGCATCCGTACTCCC
  AAAATCTCCAAGCCTATCAAGTTTGAGCTTTCTGGCTGCACCAGCATGAAGACATACCGA
  GCTAAATTCTGTGGAGTATGTACCGACGGCCGATGCTGCACCCCCCACAGAACCACCACC
  CTGCCGGTGGAGTTCAAGTGCCCTGACGGCGAGGTCATGAAGAAGAACATGATGTTCATC
  AAGACCTGTGCCTGCCATTACAACTGTCCCGGAGACAATGACATCTTTGAATCGCTGTAC
  TACAGGAAGATGTACGGAGACATGGCATGA
  
  Clone variation with respect to NM_001901.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001901 unedited
  GGGGGGGAGNNNNGTTNCTTCTCNNNNNNNGGGTCGAGAATTGTNATACCACTTATATAG
  GCGGCCGCGNAATTCGCACCGAGGNAAAGNAGACAGCCAGTGCGACTCCACCCTCCAGCT
  CGACGGCAGCCGCCCCGGCCGACAGCCCCGAGACGACAGCCCGGCGCGTCCCGGTCCCCA
  CCTCCGACCACCGCCAGCGCTCCAGGCCCCGCCGCTCCCCGCTCGCCGCCACCGCGCCCT
  CCGCTCCGCCCGCAGTGCCAACCATGACCGCCGCCAGTATGGGCCCCGTCCGCGTCGCCT
  TCGTGGTCCTCCTCGCCCTCTGCAGCCGGCCGGCCGTCGGCCAGAACTGCAGCGGGCCGT
  GCCGGTGCCCGGACGAGCCGGCGCCGCGCTGCCCGGCGGGCGTGAGCCTCGTGCTGGACG
  GCTGCGGCTGCTGCCGCGTCTGCGCCAAGCAGCTGGGCGAGCTGTGCACCGAGCGCGACC
  CCTGCGACCCGCACAAGGGCCTCTTCTGTGACTTCGGCTCCCCGGCCAACCGCAAGATCG
  GCGTGTGCACCGCCAAAGATGGTGCTCCCTGCATCTTCGGTGGTACGGTGTACCGCAGCG
  GAGAGTCCTTCCAGAGCAGCTGCAAGTACCAGTGCACGTGCCTGGACGGNGCGGTGGGCT
  GCATGCCCCTGTGCAGCATGGACGTTCGTCTGCCCAGCCCTGACTGCCCCTTCCCGAGGA
  GGGNTCAGCTGCCCNGGAAATGCTGCNNAGAGTGGGTGTGTGACAAGCCCAGGACCAAAC
  CGTGGNNTGGCCTGCCCCTCGCGCTTACCGACTGGNAGACACGTTTGGNCCCANACCCCA
  CTATGATTANAGCCAACTGCCTGGTCCAGACACAGAGTGGNAGCGCCTGTTTCAAGACTG
  TTGGATGGGCATCTTCCACCCGGGTTACCAATGACAACGCTCCTGCAGGCTANAGAAGCA
  NAGCCGCCTGTGCATGGTCAGGCCTTGCGAANCTGACCTGGAAGAGAACATTTAGAGGCC
  AAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001901 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTAAAAAAAAAAATACCCTTTTTTTTCAACTAAAAAGGGGCAAACATGTAACT
  TTTGGTCACACTTTCAACAAATAAACTGTCCGAAAAACAGTCTTTTCAAAGTAAAAACAA
  ATGCTTCCAGGGGAAAAAACTGATCAGCTTTTTAAAGTCACTCAGTTTTTAATAAAGGCC
  ATTTGTTCATTAAAAGGGAGGCTACCACATTTCCTACCTAAAAATCACCCTGCCAAGGAC
  ACTGATGCCTCCCCTTTGCAAACAATTTGTTTTGACGGACTGTCTTTTTATTTGAGCAAA
  ATTTCCATATAAGTATCCATTTCATGCTTTGAACGATCAAACAAGCTTTACATTCTACCT
  ATGGGGTTCAAAAATTGAGGCTAACATTGAAATATCAAAGCCTTAAAAACAAAAATAAAA
  AGGCCCAAACAACTTTAAATTAACTTAAATAACTGCCCCTATATTTTTTTTTCCCCACAC
  CCCCACCCCCCCAATTTTTACAATATAAATTTTAAAAAATCTGGCTTTGTACAGGCAAAT
  TCACTTGCCACAAGCTGTCCAGTTTATTCGACGGATTTCGATTCCTGACAGGGTCTTTCG
  AACAAAATGTCAGAACTGAATCTGTTGTTTTGACTTAGAGAACACTTGACTAATCCCTTG
  ATGGCTGGAAATGCCACTCTAACTGTACTGGGGCTACAGCCGGTAAGGAACCCCCTAATT
  TTCTTTTAATTACCCTTAAAAGCCGCTTTTACCCGATAAAAGCTTTCGGAGAACTACCTT
  TTTGGGGGCCCCCCCCCTGTTTTAGGCCCCCCTTATATACTTTGGGGCTGGGACAAGGGA
  ATCCCTGGAAGGTTTAACTTTTCCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001901
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001901.1, NP_001892.1
• RefSeq Size: 2312 bp
• RefSeq ORF: 1050 bp
• Locus ID: 1490
• UniProt ID: P29279
• Cytogenetics: 6q23.2
• Domains: IB, tsp_1, VWC, CT, Cys_knot
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]