Desmocollin 3 (DSC3) (NM_001941) Human Untagged Clone

CAT#: SC118960

DSC3 (untagged)-Human desmocollin 3 (DSC3), transcript variant Dsc3a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DSC3
• Synonyms: CDHF3; DSC; DSC1; DSC2; DSC4; HT-CP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001941, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGCCGCTGGGCCCCGGCGCTCCGTGCGCGGAGCCGTCTGCCTGCATCTGCTGCTGACCCTCGTGA
  TCTTCAGTCGTGCTGGTGAAGCCTGCAAAAAGGTGATACTTAATGTACCTTCTAAACTAGAGGCAGACAA
  AATAATTGGCAGAGTTAATTTGGAAGAGTGCTTCAGGTCTGCAGACCTCATCCGGTCAAGTGATCCTGAT
  TTCAGAGTTCTAAATGATGGGTCAGTGTACACAGCCAGGGCTGTTGCGCTGTCTGATAAGAAAAGATCAT
  TTACCATATGGCTTTCTGACAAAAGGAAACAGACACAGAAAGAGGTTACTGTGCTGCTAGAACATCAGAA
  GAAGGTATCGAAGACAAGACACACTAGAGAAACTGTTCTCAGGCGTGCCAAGAGGAGATGGGCACCTATT
  CCTTGCTCTATGCAAGAGAATTCCTTGGGCCCTTTCCCATTGTTTCTTCAACAAGTTGAATCTGATGCAG
  CACAGAACTATACTGTCTTCTACTCAATAAGTGGACGTGGAGTTGATAAAGAACCTTTAAATTTGTTTTA
  TATAGAAAGAGACACTGGAAATCTATTTTGCACTCGGCCTGTGGATCGTGAAGAATATGATGTTTTTGAT
  TTGATTGCTTATGCGTCAACTGCAGATGGATATTCAGCAGATCTGCCCCTCCCACTACCCATCAGGGTAG
  AGGATGAAAATGACAACCACCCTGTTTTCACAGAAGCAATTTATAATTTTGAAGTTTTGGAAAGTAGTAG
  ACCTGGTACTACAGTGGGGGTGGTTTGTGCCACAGACAGAGATGAACCGGACACAATGCATACGCGCCTG
  AAATACAGCATTTTGCAGCAGACACCAAGGTCACCTGGGCTCTTTTCTGTGCATCCCAGCACAGGCGTAA
  TCACCACAGTCTCTCATTATTTGGACAGAGAGGTTGTAGACAAGTACTCATTGATAATGAAAGTACAAGA
  CATGGATGGCCAGTTTTTTGGATTGATAGGCACATCAACTTGTATCATAACAGTAACAGATTCAAATGAT
  AATGCACCCACTTTCAGACAAAATGCTTATGAAGCATTTGTAGAGGAAAATGCATTCAATGTGGAAATCT
  TACGAATACCTATAGAAGATAAGGATTTAATTAACACTGCCAATTGGAGAGTCAATTTTACCATTTTAAA
  GGGAAATGAAAATGGACATTTCAAAATCAGCACAGACAAAGAAACTAATGAAGGTGTTCTTTCTGTTGTA
  AAGCCACTGAATTATGAAGAAAACCGTCAAGTGAACCTGGAAATTGGAGTAAACAATGAAGCGCCATTTG
  CTAGAGATATTCCCAGAGTGACAGCCTTGAACAGAGCCTTGGTTACAGTTCATGTGAGGGATCTGGATGA
  GGGGCCTGAATGCACTCCTGCAGCCCAATATGTGCGGATTAAAGAAAACTTAGCAGTGGGGTCAAAGATC
  AACGGCTATAAGGCATATGACCCCGAAAATAGAAATGGCAATGGTTTAAGGTACAAAAAATTGCATGATC
  CTAAAGGTTGGATCACCATTGATGAAATTTCAGGGTCAATCATAACTTCCAAAATCCTGGATAGGGAGGT
  TGAAACTCCCAAAAATGAGTTGTATAATATTACAGTCCTGGCAATAGACAAAGATGATAGATCATGTACT
  GGAACACTTGCTGTGAACATTGAAGATGTAAATGATAATCCACCAGAAATACTTCAAGAATATGTAGTCA
  TTTGCAAACCAAAAATGGGGTATACCGACATTTTAGCTGTTGATCCTGATGAACCTGTCCATGGAGCTCC
  ATTTTATTTCAGTTTGCCCAATACTTCTCCAGAAATCAGTAGACTGTGGAGCCTCACCAAAGTTAATGAT
  ACAGCTGCCCGTCTTTCATATCAGAAAAATGCTGGATTTCAAGAATATACCATTCCTATTACTGTAAAAG
  ACAGGGCCGGCCAAGCTGCAACAAAATTATTGAGAGTTAATCTGTGTGAATGTACTCATCCAACTCAGTG
  TCGTGCGACTTCAAGGAGTACAGGAGTAATACTTGGAAAATGGGCAATCCTTGCAATATTACTGGGTATA
  GCACTGCTCTTTTCTGTATTGCTAACTTTAGTATGTGGAGTTTTTGGTGCAACTAAAGGGAAACGTTTTC
  CTGAAGATTTAGCACAGCAAAACTTAATTATATCAAACACAGAAGCACCTGGAGACGATAGAGTGTGCTC
  TGCCAATGGATTTATGACCCAAACTACCAACAACTCTAGCCAAGGTTTTTGTGGTACTATGGGATCAGGA
  ATGAAAAATGGAGGGCAGGAAACCATTGAAATGATGAAAGGAGGAAACCAGACCTTGGAATCCTGCCGGG
  GGGCTGGGCATCATCATACCCTGGACTCCTGCAGGGGAGGACACACGGAGGTGGACAACTGCAGATACAC
  TTACTCGGAGTGGCACAGTTTTACTCAACCCCGTCTCGGTGAAAAATTGCATCGATGTAATCAGAATGAA
  GACCGCATGCCATCCCAAGATTATGTCCTCACTTATAACTATGAGGGAAGAGGATCTCCAGCTGGTTCTG
  TGGGCTGCTGCAGTGAAAAGCAGGAAGAAGATGGCCTTGACTTTTTAAATAATTTGGAACCCAAATTTAT
  TACATTAGCAGAAGCATGCACAAAGAGATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001941 unedited
  CCGCCCGTTGACGCATATGGGCGGTAGCGTGTACGGTGGNGAGTCTATATAAGCAGAGCT
  CGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGG
  CACCAGGTCTCGCTCTCGGCACCCTCCCGGCGCCCGCGTTCTCCTGGCCCTGCCCGGCAT
  CCCGATGGCCGCCGCTGGGCCCCGGCGCTCCGTGCGCGGAGCCGTCTGCCTGCATCTGCT
  GCTGACCCTCGTGATCTTCAGTCGTGCTGGTGAAGCCTGCAAAAAGGTGATACTTAATGT
  ACCTTCTAAACTAGAGGCAGACAAAATAATTGGCAGAGTTAATTTGGAAGAGTGCTTCAG
  GTCTGCAGACCTCATCCGGTCAAGTGATCCTGATTTCAGAGTTCTAAATGATGGGTCAGT
  GTACACAGCCAGGGCTGTTGCGCTGTCTGATAAGAAAAGATCATTTACCATATGGCTTTC
  TGACAAAAGGAAACAGACACAGAAAGAGGTTACTGTGCTGCTAGAACATCAGAAGAGGTA
  TCGAAGACAAGACACACTAGAGAAACTGTTCTCANGCGTGCCCAAGAGAGATGGGCACCT
  ATTCCTTGCTCTATGNCAGAGAATTCNNTGGGCCCTTTNCCATTTGTTTCTCAACAGTTG
  AATCTGATGCAGCACAGAACTATACTGTCTTCTACTCAATAGTGGACGTGGAGTTGAAAA
  GAACCTTTTAAATTGNTTTATATAGAAAGAACCCTGGAAATCTATTTGCACTCGGCCTGT
  GNATCCGGAAGAATTGATGTTNTTGAATTGAATGCCTATGCGCAACTGGCAATGGATATC
  CACCAAATCTGCCCTTCCCTTACCCTTCGGTTAAAGAATGAAAAGCAAACCCCCCGGTTT
  CCAAAAAACAATTTTAATTTTGAAATTTTGGAAAANAAAAACCTGTCCTACAGGGGGGGG
  GTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001941 unedited
  CGTGGACCATTGGCGATGGCCACTTCCCAGGNCCAGNATAGCACTGGGGAAGGGTGTCAC
  AGGGCATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGT
  CGAGTTTTTTTTTTTTTTTTTTAAATGTATCCGGTATTTTACTATTACAAACAAAAATCC
  AATGAACATTCTTGAAGACATACACAAAAATAATGGTTACAATAGAAGTTACTGGAATTG
  AAATTTTGGTTCAACCTATATTAAAATGTAAGGCTTTTGATATAGCTAATAGATTTTTGA
  AATGATCAGTCTTAACGTTTGTAGGGGAGCACACTCCTGCATGGGGAAAAGATTCACTGT
  GAAGCACAGAGCACCTTTATGGTTGGATCATCTTGTCATTAAAGTTCAGGCATTATCTAT
  CCTGTAAGTGGCAAAATCAAGACTGCAATATTGCCTGCTTTTCTTTTTAACTCATGTTTT
  CCCTTGACTACACTGGTCCTCAAAGTAAAACCCCTGTGTCAGTGTACTATTCATGGAATA
  CTCTGCCATTATAACCCCCCTTCTAATACTTTTAATACCCGATCAAATTTTATTATACCT
  TATGTATCATACATTCCCTCACCGTAAATCTGCGCTTCAATAACGCGATTTCCTCCCACC
  TTTACTTTTCCTTTTACCTGATCTTCATTTTCTTGCATTCCTCTATCTGCCCTATATATC
  CCCTTTTCTTTTACTCCTCCATTTTTTTGCCCATTTTTCTCTCCTCTACTCCCCACACCC
  CCCCCCTCTTCATTTTTCTCCCATCTATTCTTCCTTTCACCCCTTCACTTATCCCCTATC
  CTATCTTTCCCCGTATTTTCCCTCTCTCCTTACCCCCGTCCCCGTATTTCCTTCTTCCCC
  TTTTCCCCGTTCCTTATCTCATTTTCCCTTCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001941
• Insert Size: 5700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001941.2, NP_001932.1
• RefSeq Size: 5879 bp
• RefSeq ORF: 2691 bp
• Locus ID: 1825
• UniProt ID: Q14574
• Cytogenetics: 18q12.1
• Domains: Cadherin_C_term, CA
• Protein Families: Transmembrane
• Gene Summary: The protein encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in this gene are a cause of hypotrichosis and recurrent skin vesicles disorder. The protein can act as an autoantigen in pemphigus diseases, and it is also considered to be a biomarker for some cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014]
  Transcript Variant: This variant (Dsc3a) represents the shorter transcript but encodes the longer isoform (Dsc3a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.