GCLM (NM_002061) Human Untagged Clone

CAT#: SC118877

GCLM (untagged)-Human glutamate-cysteine ligase, modifier subunit (GCLM)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GCLM
• Synonyms: GLCLR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118877 sequence for NM_002061 edited (data generated by NextGen Sequencing)
  ATGGGCACCGACAGCCGCGCGGCCAAGGCGCTCCTGGCGCGGGCCCGCACCCTGCACCTG
  CAGACGGGGAACCTGCTGAACTGGGGCCGCCTGCGGAAGAAGTGCCCGTCCACGCACAGC
  GAGGAGCTTCATGATTGTATCCAAAAAACCTTGAATGAATGGAGTTCCCAAATCAACCCA
  GATTTGGTCAGGGAGTTTCCAGATGTCTTGGAATGCACTGTATCTCATGCAGTAGAAAAG
  ATAAATCCTGATGAAAGAGAAGAAATGAAAGTTTCTGCAAAACTGTTCATTGTAGAATCA
  AACTCTTCATCATCAACTAGAAGTGCAGTTGACATGGCCTGTTCAGTCCTTGGAGTTGCA
  CAGCTGGATTCTGTGATCATTGCTTCACCTCCTATTGAAGATGGAGTTAATCTTTCCTTG
  GAGCATTTACAGCCTTACTGGGAGGAATTAGAAAACTTAGTTCAGAGCAAAAAGATTGTT
  GCCATAGGTACCTCTGATCTAGACAAAACACAGTTGGAACAGCTGTATCAGTGGGCACAG
  GTAAAACCAAATAGTAACCAAGTTAATCTTGCCTCCTGCTGTGTGATGCCACCAGATTTG
  ACTGCATTTGCTAAACAATTTGACATACAGCTGTTGACTCACAATGATCCAAAAGAACTG
  CTTTCTGAAGCAAGTTTCCAAGAAGCTCTTCAGGAAAGCATTCCTGACATTCAAGCGCAC
  GAGTGGGTGCCGCTGTGGCTACTGCGGTATTCGGTCATTGTGAAAAGTAGAGGAATTATC
  AAATCAAAAGGCTACATTTTACAAGCTAAAAGAAGGGGTTCTTAA
  
  Clone variation with respect to NM_002061.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002061 unedited
  GGTTCAAATTTGTATACGACTCATATAGGCGGCCGCCGCGCGGCCGCCTGCCTGCCCGCC
  CGCCCGCAGCCCCTTCGCTGCGCGGCCCCTGGGCGGCCGCTGCCATGGGCACCGACAGCC
  GCGCGGCCAAGGCGCTCCTGGCGCGGGCCCGCACCCTGCACCTGCAGACGGGGAACCTGC
  TGAACTGGGGCCGCCTGCGGAAGAAGTGCCCGTCCACGCACAGCGAGGAGCTTCATGATT
  GTATCCAAAAAACCTTGAATGAATGGAGTTCCCAAATCAACCCAGATTTGGTCAGGGAGT
  TTCCAGATGTCTTGGAATGCACTGTATCTCATGCAGTAGAAAAGATAAATCCTGATGAAA
  GAGAAGAAATGAAAGTTTCTGCAAAACTGTTCATTGTAGAATCAAACTCTTCATCATCAA
  CTAGAAGTGCAGTTGACATGGCCTGTTCAGTCCTTGGAGTTGCACAGCTGGATTCTGTGA
  TCATTGCTTCACCTCCTATTGAAGATGGAGTTAATCTTTCCTTGGAGCATTTACAGCCTT
  ACTGGGAGGAATTAGAAAACTTAGTTCAGAGCAAAAAGATTGTTGCCATAGGTACCTCTG
  ATCTAGACAAAACACAGTTGGAACAGCTGTATCAGTGGGCACAGGTAAAACCAAATAGTA
  ACCAAGTTAATCTTGCCTCCTGCTGTGTGATGCCACCAGATTTGACTGCATTTGCTAAAC
  AATTTGACATACAGCTGTTGACTCACAATGATCCAAAAGAACTGCTTTCTGAAGCAAGTT
  CCAAGAAGCTCTTCAGAAAGCATTCCTGACATTCAGCGCACGAANTGGGTGCCGCTGTGG
  CTACTGGCGTATTTCGGTCATTGNGGAAAGTNNAGGAATATCAATCAAAGGCTACTTNTA
  CAGCTAAGAAAGGGGTCTNAACTGACTAGAGCATACTACTTGGAATNCCTCCATAGANAA
  A
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002061 unedited
  GGGGGCGGGNTGTCCTTCTCCCCCCCGGCAGATTTGAACCCGGCCGCATCTTATGATCGA
  TTTTTTTTTTTTTTTTTTGAAAAGGAGTCTCGCTTTGTCGCCCAGGCTGGAGTGCAGTGA
  TGTGATCTCGGCTCACTGTAACCTCCGCCTCCCGGGTTCAAGNGATTNTCCTGCCTTAGC
  CTCCCAAGTACCTGATATTACAAGCGAGTGCCACCACGCCCAGCCAATTTTTGTATTTTA
  GCCACAGATGCGGCCTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCGCGATC
  TGCCCACCTCCGCCCCACAAAGTGCTGGTATTACAGGTGCCAGCCACTGCGCCCGCACGA
  AATACAACAAATTTTCAAAAGAAAGATTTCACACTATTCAGCGTTACCACACCCCAATTT
  ATACACATAACGATACTTCATAGCGCACATCACCAGCTCATACCCTATCTATTCGCCAAT
  GTTGACGCCCCGAAAACCAATGCACCAATTAACTTACCTGACTTTTACATTTTTATTCCA
  CCTACAAACCTCTCACTCCTACCACTACTCCCCCTACAACACCCCACACCATTATCTTAA
  AACTCGTTCCACCTCTATAAAGTACTGACCCGCAATCAAGCAATTCTTTTTCACCCCCCC
  CATCTGGCCCTTAAACCCCTCCACAACCACCCCACCACACGGGGGAAAAATAAACCTACC
  ACACTTTCACTAAATAAGAATCTAAATTCCTACAATCTAATTGCCCCCTACCTTTTTCTC
  CAACGCTCTCTACGAATTCACAAACATCGCAACAAACCATTTTAATCTTCCCTCCACGCC
  TCTTATTCACCACTCACCCCAAACCAACACCCCTTCCATGTGCATCAACATATCATTATT
  AACCAAATNCCGGATATGCACCGTCCCATAGCACTCTCCTCCCCTTTACCACAACACTCC
  CTACAACTACTACCTTTTCATAAGAGCTGTCTAAGTCCTCTCTCCTCCAAANATAATAAC
  TCTTCGCCTATCTTCTCTCTCTCCCATCACGCCACCACTATCACACAATCAGATCGCGAC
  CTCAAACAATCTNAAAGTGTCCAATCTATATCACACACTCTTCATCAACTCTCTTAACCA
  TAATACACATCTNTCGGAATATACATCACACCTATCCCTCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002061
• Insert Size: 2630 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002061.2, NP_002052.1
• RefSeq Size: 3074 bp
• RefSeq ORF: 825 bp
• Locus ID: 2730
• UniProt ID: P48507
• Cytogenetics: 1p22.1
• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism, Metabolic pathways
• Gene Summary: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).