GAP43 (NM_002045) Human Untagged Clone

CAT#: SC118868

GAP43 (untagged)-Human growth associated protein 43 (GAP43), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GAP43
• Synonyms: B-50; GAP-43; PP46
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002045 edited
  GAATTCGGCACGAGGGGAAGAAGGCAAGGGACGAGACAACCATGCTGTGCTGTATGAGAA
  GAACCAAACAGGTTGAAAAAAATGATGACGACCAAAAGATTGAACAAGATGGTATCAAAC
  CAGAAGATAAAGCTCATAAGGCCGCAACCAAAATTCAGGCTAGCTTCCGTGGACACATAA
  CAAGGAAAAAGCTCAAAGGAGAGAAGAAGGATGATGTCCAAGCTGCTGAGGCTGAAGCTA
  ATAAGAAGGATGAAGCCCCTGTTGCCGATGGGGTGGAGAAGAAGGGAGAAGGCACCACTA
  CTGCCGAAGCAGCCCCAGCCACTGGCTCCAAGCCTGATGAGCCCGGCAAAGCAGGAGAAA
  CTCCTTCCGAGGAGAAGAAGGGGGAGGGTGATGCTGCCACAGAGCAGGCAGCCCCCCAGG
  CTCCTGCATCCTCAGAGGAGAAGGCCGGCTCAGCTGAGACAGAAAGTGCCACTAAAGCTT
  CCACTGATAACTCGCCGTCCTCCAAGGCTGAAGATGCCCCAGCCAAGGAGGAGCCTAAAC
  AAGCCGATGTGCCTGCTGCTGTCACTGCTGCTGCTGCCACCACCCCTGCCGCAGAGGATG
  CTGCTGCCAAGGCAACAGCCCAGCCTCCAACGGAGACTGGGGAGAGCAGCCAAGCTGAAG
  AGAACATAGAAGCTGTAGATGAAACCAAACCTAAGGAAAGTGCCCGGCAGGACGAGGGTA
  AAGAAGAGGAACCTGAGGCTGACCAAGAACATGCCTGAACTCTAAGAAATGGCTTTCCAC
  ATCCCCACCCTCCCCTCTCCTGAGCCTGTCTCTCCCTACCCTCTTCTCAGCTCCACTCTG
  AAGTCCCTTCCTGTCCTGCTCACGTCTGTGAGTCTGTCCTTTCCCACCCACTAGCCCTCT
  TTCTCTCTGTGTGGCAAACATTAAAAAAAAAAAAAAAAGCAGGAAAGATCCCAAGTCAAA
  CAGTGTGGCTTAAACATTTTTTGTTTCTTGGTGTTGTTATGGCAAGTTTTTGGTAATGAT
  GATTCAATCATTTGGGGAAATTCTTGCACTGTATCCAAGTTATTTGATCTGGTGCGTGTG
  GCCCTGTGGGAGTCCACTTTCCTCTCTCTCTCTCTCTCTGTTCCAAGTGTGTGTGCAATG
  TTCCGTTCATCTGAGGAGTCCAAAATATCGAGTGAATTCAAAATCATTTTTGTTTTCCTC
  CTTTTCAATGTGATGGAATGAACAAAAAGGAAAAAATTCAAAAAACCCAGTTTGTTTTAA
  AAATAAATAAATAAAGCAAATGTGCCAATTAGCGTAAACTTGCGGCTCTAAGGCTCCTTT
  TTCAACCCGAATATTAATAAATCATGAGAGTAATCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002045 unedited
  GTGGATTTTGTAATACGATTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAAGAAG
  TCAGGGACGAGACAACCATGCTGTGCTGTATGAGAAGAAACCAAACAGGTTGAAAAAAAT
  GATGACGACCAAAAGATTGAACAAGATGGTATCAAACCAGAAGATAAAGCTCATAAGGCC
  GCAACCAAAATTCAGGCTAGCTTCCGTGGACACATAACAAGGAAAAAGCTCAAAGGAGAG
  AAGAAGGATGATGTCCAAGCTGCTGAGGCTGAAGCTAATAAGAAGGATGAAGCCCCTGTT
  GCCGATGGGGTGGAGAAGAAGGGAGAAGGCACCACTACTGCCGAAGCAGCCCCAGCCACT
  GGCTCCAAGCCTGATGAGCCCGGCAAAGCAGGAGAAACTCCTTCCGAGGAGAAGAAGGGG
  GAGGGTGATGCTGCCACAGAGCAGGCAGCCCCCCAGGCTCCTGCATCCTCAGAGGAGAAG
  GCCGGCTCAGCTGAGACAGAAAGTGCCACTAAAGCTTCCACTGATAACTCGCCGTCCTCC
  AAGGCTGAAGATGCCCCAGCCAAGGAGGAGCCTAAACAAGCCGATGTGCCTGCTGCTGTC
  ACTGCTGCTGCTGCCACCACCCCTGCCGCAGAGGATGCTGCTGCCAAGGCAACAGCCCAG
  CCTCCAACGGAGACTGGGGAGAGCAGCCAAGCTGAAGAGACATAGAAGCTGTAGATGAAA
  CCANACCTAAAGAAAGTGCCCCGCAGGACGAGGGTTATGAAGAGGACCCTGAGCTGACCA
  AGACATGCCTGACTCTAAGAAATGGCTTTCACATNCCACCCTCCCTTTCTGAGCCTGGTC
  TCCCTACCCTCTNTCAGCTCACTCTGAAGCCCTTCCGTCCTGCTAAGTTTGTGAGTTTGT
  CCTTTCCACCCACTACCCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002045 unedited
  GCAACTTTCCAGGGCCAGGNAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATC
  TGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT
  TTGATTACTCTCATGATTTATTAATATTCGGGTTGAAAAAGGAGCCTTAGAGCCGCAAGT
  TTACGCTAATTGGCACATTTGCTTTATTTATTTATTTTTAAAACAAACTGGGTTTTTTGA
  ATTTTTTCCTTTTTGTTCATTCCATCACATTGAAAAGGAGGAAAACAAAAATGATTTTGA
  ATTCACTCGATATTTTGGACTCCTCAGATGAACGGAACATTGCACACACACTTGGAACAG
  AGAGAGAGAGAGAGAGGAAAGTGGACTCCCACAGGGCCACACGCACCAGATCAAATAACN
  NNGATACAGTGCAAGAATTTCCCCAAATGATTGAATCATCATTACCAAAAACTTGCCATA
  ACAACACCAAGAAACAAAAAATGTTTAAGCCACACTGTTTGACTTGGGATCTTTCCTGCT
  TTTTTTTTTTTTTTTAATGTTTGCCACACAGAGAGAAAGAGGGCTAGTGGGTGGGAAAGG
  ACAGACTCACAGACGTGAGCAGGACAGGAAGGGACTTCANAGTGGAGCTGAGAAAAGGGT
  AGGGAGAGACAGGCTCANGAGAGGGAGGGTGGGGGATGTGAAAGCCATTTCTTAGAGTTC
  AGGCATGTTCTTGGTCGCCTTTNNNNTCTCTTTTTACCCTCGTCCTGCCGGGCACTTNCT
  TTAGGTTTGGTTCATCTACAGCTTCTATGGTCTCTTTAGCTTGGCTGCTCTCCCCAGTCT
  CGTTGGAAGCTGGGCTGGTGCCTTGGCACCACATCCTCTGCGCAGGGGTGGTGGCACACA
  ACAGTACAGCACAGGCCATCGCTTGNTAGGCTCTNCTGGCTGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002045
• Insert Size: 1470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002045.2, NP_002036.1
• RefSeq Size: 1225 bp
• RefSeq ORF: 717 bp
• Locus ID: 2596
• UniProt ID: P17677
• Cytogenetics: 3q13.31
• Domains: IQ
• Gene Summary: The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an internal exon and has an upstream AUG start codon, as compared to variant 1. The resulting isoform 2 has a shorter and distinct N-terminus, as compared to variant 1.