GAP43 (NM_002045) Human Untagged Clone

SKU SC118868

GAP43 (untagged)-Human growth associated protein 43 (GAP43), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: GAP43
• Synonyms: B-50; GAP-43; PP46
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002045 edited; GAATTCGGCACGAGGGGAAGAAGGCAAGGGACGAGACAACCATGCTGTGCTGTATGAGAA; GAACCAAACAGGTTGAAAAAAATGATGACGACCAAAAGATTGAACAAGATGGTATCAAAC; CAGAAGATAAAGCTCATAAGGCCGCAACCAAAATTCAGGCTAGCTTCCGTGGACACATAA; CAAGGAAAAAGCTCAAAGGAGAGAAGAAGGATGATGTCCAAGCTGCTGAGGCTGAAGCTA; ATAAGAAGGATGAAGCCCCTGTTGCCGATGGGGTGGAGAAGAAGGGAGAAGGCACCACTA; CTGCCGAAGCAGCCCCAGCCACTGGCTCCAAGCCTGATGAGCCCGGCAAAGCAGGAGAAA; CTCCTTCCGAGGAGAAGAAGGGGGAGGGTGATGCTGCCACAGAGCAGGCAGCCCCCCAGG; CTCCTGCATCCTCAGAGGAGAAGGCCGGCTCAGCTGAGACAGAAAGTGCCACTAAAGCTT; CCACTGATAACTCGCCGTCCTCCAAGGCTGAAGATGCCCCAGCCAAGGAGGAGCCTAAAC; AAGCCGATGTGCCTGCTGCTGTCACTGCTGCTGCTGCCACCACCCCTGCCGCAGAGGATG; CTGCTGCCAAGGCAACAGCCCAGCCTCCAACGGAGACTGGGGAGAGCAGCCAAGCTGAAG; AGAACATAGAAGCTGTAGATGAAACCAAACCTAAGGAAAGTGCCCGGCAGGACGAGGGTA; AAGAAGAGGAACCTGAGGCTGACCAAGAACATGCCTGAACTCTAAGAAATGGCTTTCCAC; ATCCCCACCCTCCCCTCTCCTGAGCCTGTCTCTCCCTACCCTCTTCTCAGCTCCACTCTG; AAGTCCCTTCCTGTCCTGCTCACGTCTGTGAGTCTGTCCTTTCCCACCCACTAGCCCTCT; TTCTCTCTGTGTGGCAAACATTAAAAAAAAAAAAAAAAGCAGGAAAGATCCCAAGTCAAA; CAGTGTGGCTTAAACATTTTTTGTTTCTTGGTGTTGTTATGGCAAGTTTTTGGTAATGAT; GATTCAATCATTTGGGGAAATTCTTGCACTGTATCCAAGTTATTTGATCTGGTGCGTGTG; GCCCTGTGGGAGTCCACTTTCCTCTCTCTCTCTCTCTCTGTTCCAAGTGTGTGTGCAATG; TTCCGTTCATCTGAGGAGTCCAAAATATCGAGTGAATTCAAAATCATTTTTGTTTTCCTC; CTTTTCAATGTGATGGAATGAACAAAAAGGAAAAAATTCAAAAAACCCAGTTTGTTTTAA; AAATAAATAAATAAAGCAAATGTGCCAATTAGCGTAAACTTGCGGCTCTAAGGCTCCTTT; TTCAACCCGAATATTAATAAATCATGAGAGTAATCAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002045 unedited; GTGGATTTTGTAATACGATTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAAGAAG; TCAGGGACGAGACAACCATGCTGTGCTGTATGAGAAGAAACCAAACAGGTTGAAAAAAAT; GATGACGACCAAAAGATTGAACAAGATGGTATCAAACCAGAAGATAAAGCTCATAAGGCC; GCAACCAAAATTCAGGCTAGCTTCCGTGGACACATAACAAGGAAAAAGCTCAAAGGAGAG; AAGAAGGATGATGTCCAAGCTGCTGAGGCTGAAGCTAATAAGAAGGATGAAGCCCCTGTT; GCCGATGGGGTGGAGAAGAAGGGAGAAGGCACCACTACTGCCGAAGCAGCCCCAGCCACT; GGCTCCAAGCCTGATGAGCCCGGCAAAGCAGGAGAAACTCCTTCCGAGGAGAAGAAGGGG; GAGGGTGATGCTGCCACAGAGCAGGCAGCCCCCCAGGCTCCTGCATCCTCAGAGGAGAAG; GCCGGCTCAGCTGAGACAGAAAGTGCCACTAAAGCTTCCACTGATAACTCGCCGTCCTCC; AAGGCTGAAGATGCCCCAGCCAAGGAGGAGCCTAAACAAGCCGATGTGCCTGCTGCTGTC; ACTGCTGCTGCTGCCACCACCCCTGCCGCAGAGGATGCTGCTGCCAAGGCAACAGCCCAG; CCTCCAACGGAGACTGGGGAGAGCAGCCAAGCTGAAGAGACATAGAAGCTGTAGATGAAA; CCANACCTAAAGAAAGTGCCCCGCAGGACGAGGGTTATGAAGAGGACCCTGAGCTGACCA; AGACATGCCTGACTCTAAGAAATGGCTTTCACATNCCACCCTCCCTTTCTGAGCCTGGTC; TCCCTACCCTCTNTCAGCTCACTCTGAAGCCCTTCCGTCCTGCTAAGTTTGTGAGTTTGT; CCTTTCCACCCACTACCCTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002045 unedited; GCAACTTTCCAGGGCCAGGNAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATC; TGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT; TTGATTACTCTCATGATTTATTAATATTCGGGTTGAAAAAGGAGCCTTAGAGCCGCAAGT; TTACGCTAATTGGCACATTTGCTTTATTTATTTATTTTTAAAACAAACTGGGTTTTTTGA; ATTTTTTCCTTTTTGTTCATTCCATCACATTGAAAAGGAGGAAAACAAAAATGATTTTGA; ATTCACTCGATATTTTGGACTCCTCAGATGAACGGAACATTGCACACACACTTGGAACAG; AGAGAGAGAGAGAGAGGAAAGTGGACTCCCACAGGGCCACACGCACCAGATCAAATAACN; NNGATACAGTGCAAGAATTTCCCCAAATGATTGAATCATCATTACCAAAAACTTGCCATA; ACAACACCAAGAAACAAAAAATGTTTAAGCCACACTGTTTGACTTGGGATCTTTCCTGCT; TTTTTTTTTTTTTTTAATGTTTGCCACACAGAGAGAAAGAGGGCTAGTGGGTGGGAAAGG; ACAGACTCACAGACGTGAGCAGGACAGGAAGGGACTTCANAGTGGAGCTGAGAAAAGGGT; AGGGAGAGACAGGCTCANGAGAGGGAGGGTGGGGGATGTGAAAGCCATTTCTTAGAGTTC; AGGCATGTTCTTGGTCGCCTTTNNNNTCTCTTTTTACCCTCGTCCTGCCGGGCACTTNCT; TTAGGTTTGGTTCATCTACAGCTTCTATGGTCTCTTTAGCTTGGCTGCTCTCCCCAGTCT; CGTTGGAAGCTGGGCTGGTGCCTTGGCACCACATCCTCTGCGCAGGGGTGGTGGCACACA; ACAGTACAGCACAGGCCATCGCTTGNTAGGCTCTNCTGGCTGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002045
• Insert Size: 1470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002045.2, NP_002036.1
• RefSeq Size: 1225 bp
• RefSeq ORF: 717 bp
• Locus ID: 2596
• UniProt ID: P17677
• Cytogenetics: 3q13.31
• Domains: IQ
• Summary: The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) lacks an internal exon and has an upstream AUG start codon, as compared to variant 1. The resulting isoform 2 has a shorter and distinct N-terminus, as compared to variant 1.