Glutamine Synthetase (GLUL) (NM_002065) Human Untagged Clone

CAT#: SC118847

GLUL (untagged)-Human glutamate-ammonia ligase (GLUL), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Glutamine Synthetase
• Synonyms: GLNS; GS; PIG43; PIG59
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002065 edited
  GAATTCGGCACGAGGGCGGAGCGTGTGAGCAGTACTGCGGCCTCCTCTCCTCTCCTAACC
  TCGCTCTCGCGGCCTAGCTTTACCCGCCCGCCTGCTCGGCGACCAGAACACCTTCCACCA
  TGACCACCTCAGCAAGTTCCCACTTAAATAAAGGCATCAAGCAGGTGTACATGTCCCTGC
  CTCAGGGTGAGAAAGTCCAGGCCATGTATATCTGGATCGATGGTACTGGAGAAGGACTGC
  GCTGCAAGACCCGGACCCTGGACAGTGAGCCCAAGTGTGTGGAAGAGTTGCCTGAGTGGA
  ATTTCGATGGCTCTAGTACTTTACAGTCTGAGGGTTCCAACAGTGACATGTATCTCGTGC
  CTGCTGCCATGTTTCGGGACCCCTTCCGTAAGGACCCTAACAAGCTGGTGTTATGTGAAG
  TTTTCAAGTACAATCGAAGGCCTGCAGAGACCAATTTGAGGCACACCTGTAAACGGATAA
  TGGACATGGTGAGCAACCAGCACCCCTGGTTTGGCATGGAGCAGGAGTATACCCTCATGG
  GGACAGATGGGCACCCCTTTGGTTGGCCTTCCAACGGCTTCCCAGGGCCCCAGGGTCCAT
  ATTACTGTGGTGTGGGAGCAGACAGAGCCTATGGCAGGGACATCGTGGAGGCCCATTACC
  GGGCCTGCTTGTATGCTGGAGTCAAGATTGCGGGGACTAATGCCGAGGTCATGCCTGCCC
  AGTGGGAATTTCAGATTGGACCTTGTGAAGGAATCAGCATGGGAGATCATCTCTGGGTGG
  CCCGTTTCATCTTGCATCGTGTGTGTGAAGACTTTGGAGTGATAGCAACCTTTGATCCTA
  AGCCCATTCCTGGGAACTGGAATGGTGCAGGCTGCCATACCAACTTCAGCACCAAGGCCA
  TGCGGGAGGAGAATGGTCTGAAGTACATCGAGGAGGCCATTGAGAAACTAAGCAAGCGGC
  ACCAGTACCACATCCGTGCCTATGATCCCAAGGGAGGCCTGGACAATGCCCGACGTCTAA
  CTGGATTCCATGAAACCTCCAACATCAACGACTTTTCTGCTGGTGTAGCCAATCGTAGCG
  CCAGCATACGCATTCCCCGGACTGTTGGCCAGGAGAAGAAGGGTTACTTTGAAGATCGTC
  GCCCCTCTGCCAACTGCGACCCCTTTTCGGTGACAGAAGCCCTCATCCGCACGTGTCTTC
  TCAATGAAACCGGCGATGAGCCCTTCCAGTACAAAAATTAAGTGGACTAGACCTCCAGCT
  GTTGAGCCCCTCCTAGTTCTTCATCCCACTCCAACTCTTCCCCCTCTCCCAGTTGTCCCG
  ATTGTAACTCAAAGGGTGGAATATCAAGGTCGTTTTTTTCATTCCAAAAAAAAAAAAAAA
  AAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002065 unedited
  ATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCGGAGCGTGTGAGC
  AGTACTGCGGCCTCCTCTCCTCTCCTAACCTCGCTCTCGCGGCCTAGCTTTACCCGCCCG
  CCTGCTCGGCGACCAGAACACCTTCCACCATGACCACCTCAGCAAGTTCCCACTTAAATA
  AAGGCATCAAGCAGGTGTACATGTCCCTGCCTCAGGGTGAGAAAGTCCAGGCCATGTATA
  TCTGGATCGATGGTACTGGAGAAGGACTGCGCTGCAAGACCCGGACCCTGGACAGTGAGC
  CCAAGTGTGTGGAAGAGTTGCCTGAGTGGAATTTCGATGGCTCTAGTACTTTACAGTCTG
  AGGGTTCCAACAGTGACATGTATCTCGTGCCTGCTGCCATGTTTCGGGACCCCTTCCGTA
  AGGACCCTAACAAGCTGGTGTTATGTGAAGTTTTCAAGTACAATCGAAGGCCTGCAGAGA
  CCAATTTGAGGCACACCTGTAAACGGATAATGGACATGGTGAGCAACCAGCACCCCTGGT
  TTGGCATGGAGCAGGAGTATACCCTCATGGGGACAGATGGGCACCCCTTTGGTTGGCCTT
  CCAACGGCTTCCCAGGGCCCCAGGGTCCATATTACTGTGGTGTGGGAGCAGACAGAGCCT
  ATGGCAGGGACATCGTGGAGGCCCATTACCGGGCCTGCTTGTATGCTGGAGTCAAGATTG
  CGGNGAACTAATGCCGAGTCATGCCTGCCCAGTGGGAATTTCAGATTGGACCTTGTGAAG
  GAATCAGCATGGGAGATCATCTCTGGGTGGCCCGTTTCATCTTGCATCGTGTGTGTGAAG
  AACTTGGAGTGATAGCACCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002065 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGNTTTTTTTTTTTTTTTTTTTTTTGGAATGAAAAA
  AACGACCTTGATATTCCACCCTTTGAGTTACAATCGGGACAACTGGGAGAGGGGGAAGAG
  TTGGAGTGGGATGAAAAACTAGGAGGGGCTCAACAGCTGGAGGTCTAGTCCACTTAATTT
  TTGTACTGGAAGGGCTCATCGCCGGTTTCATTGAGAAGACACGTGCGGATGAGGGCTTCT
  GTCACCGAAAAGGGGTCGCAGTTGGCAGAGGGGCGACGATCTTCAAAGTAACCCTTCTTC
  TCCTGGCCAACAGTCCGGGGAATGCGTATGCTGGCGCTACCATTGGCTACACCAGCAGAA
  AAGTCGTTGATGTTGGAGGTTTCATGGAATCCAGTTAGACGTCGGGCATTGTCCAGGCCT
  CCCTTGGGATCATAGGCACGGATGTGGTACTGGTGCCGCTTGCTTAGTTTCTCAATGGCC
  TCCTCGATGTACTTCAGACCATTCTCCTCCCGCATGGCCTTGGTGCTGAAGTTGGTATGG
  CAGCCTGCACCATTCCAGTTCCCAGGAATGGGCTTAGGATCAAAGGTTGCTATCACTCCA
  AAGTCTTCACACACACGATGCAAGATGAAACGGGCCACCCAGAGATGATCTNCCATGCTG
  ATTCCTTCACAAGGTCCAATCTGAAATTCCACTGGGCAGGCATGACCTCCGCATTAGTCC
  CCGCAATCTTGACTCCAGCATACAAGCAAGCCCGGTAATGGGCCCTCCACGATGTCCCTG
  GCCTAGGCTCTGCCTGCTCCACACCCCAGTAATTGGACCCTGGGGCCCTGGAAGCCCCTC
  GGAGGCCACCCAAGGGGTGCCATCTGGCCCACTGAGGGTACTCCTGTCCTGCCAAACACG
  GGGGCCGGTGGCTACCTGTCCTTATCCGTTACGGGGGCCTAAATGGGCTTGAGGCCTCAT
  TGGCCTGAAACTTCCATACACCACTGGTAGGGCCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002065
• Insert Size: 1430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002065.4, NP_002056.2
• RefSeq Size: 3499 bp
• RefSeq ORF: 1122 bp
• Locus ID: 2752
• UniProt ID: P15104
• Cytogenetics: 1q25.3
• Domains: gln-synt, gln-synt_N
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways, Nitrogen metabolism
• Gene Summary: The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
  Transcript Variant: This variant (1) represents the longest transcript. Both variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.