Cpn10 (HSPE1) (NM_002157) Human Untagged Clone

SKU SC118785

HSPE1 (untagged)-Human heat shock 10kDa protein 1 (chaperonin 10) (HSPE1), nuclear gene encoding mitochondrial protein

Product Data

• Type: Human Untagged Clone
• Target Symbol: Cpn10
• Synonyms: CPN10; EPF; GROES; HSP10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002157 edited; ATGGCAGGACAAGCGTTTAGAAAGTTTCTTCCACTCTTTGACCGAGTATTGGTTGAAAGG; AGTGCTGCTGAAACTGTAACCAAAGGAGGCATTATGCTTCCAGAAAAATCTCAAGGAAAA; GTATTGCAAGCAACAGTAGTCGCTGTTGGATCGGGTTCTAAAGGAAAGGGTGGAGAGATT; CAACCAGTTAGCGTGAAAGTTGGAGATAAAGTTCTTCTCCCAGAATATGGAGGCACCAAA; GTAGTTCTAGATGACAAGGATTATTTCCTATTTAGAGATGGTGACATTCTTGGAAAGTAC; GTAGACTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002157 unedited; ATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGTGCGGCGCTACACTAGAGCAGA; GTACGAGTCTGAGGCGGAGGGAGTAATGGCAGGACAAGCGTTTAGAAAGTTTCTTCCACT; CTTTGACCGAGTATTGGTTGAAAGGAGTGCTGCTGAAACTGTAACCAAAGGAGGCATTAT; GCTTCCAGAAAAATCTCAAGGAAAAGTATTGCAAGCAACAGTAGTCGCTGTTGGATCGGG; TTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGGAGATAAAGTTCT; TCTCCCAGAATATGGAGGCACCAAAGTAGTTCTAGATGACAAGGATTATTTCCTATTTAG; AGATGGTGACATTCTTGGAAAGTACGTAGACTGAAATAAGTCACTATTGAAATGGCATCA; ACATGATGCTGCCCATTCCACTGAAGTTCTGAAATCTTTCGTCATGTAAATAATTTCCAT; ATTTCTCTTTTATAATAAACTAATGATAACTAATGACAAANAAAAAAAAAAAAAAAAAAA; NNCCTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGC; ATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGNAAGTTGCCACTCCAGTGCCCA; CCAGCCTTGTCCTAAAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAT; ATTATGGGGTGAGGGNGGTGGGTATGGAGCAGGGTGCNNAGTGGGAAAACACCTGTAGGN; CCCTGCGGGTCTATTGGGAACAAGCTGAAGTGCAGTGCACAATCTTGGCTCACTGCATCT; CCGCTNCTGGGTTCAGCGATTCTCCTGCTCAGCCTCCGGATTGTTGGGATTCCAGNCTGC; ATGACCAGCTCACTAATTTTGTTTTTTGTAGAAACGGGTTTACCATATGGCCAGCTGTT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002157 unedited; NNTTTCGCTCTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT; TTTTTGGCATTAGTTATCATTAGTTTATTATAAAAGAAAAATTGGAAATTATTTACATGA; CGAAAGATTTCAGAACTTCAGTGGAATGGGCAGCATCATGTTGATGCCATTTCAATAGTG; ACTTATTTCAGTCTACGTACTTTCCAAGAATGTCACCATCTCTAAATAGGAAATAATCCT; TGTCATCTAGAACTACTTTGGTGCCTCCATATTCTGGGAGAAGAACTTTATCTCCAACTT; TCACGCTAACTGGTTGAATCTCTCCACCCTTTCCTTTAGAACCCGATCCAACAGCGACTA; CTGGTGCTTGCAATACTTTTCCTTGAGATTTTTCTGGAAGCATAATGCCTCCTTTGGTTA; CAGTTTCAGCAGCACTCCTTTCAACCAATACTCGGTCAAAGAGTGGAAGAAACTTTCTAA; ACGCTTGTCCTGCCATTACTCCCTCCGCCTCAGACTCGTACTCTGCTCTAGTGTAGCGCC; GCACCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGT; TCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCG; TCAACGGGGCGCGNGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAAC; AAACTCCCATTGACGTCAATGGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTA; TCCACGCCCATTTGGTGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATA; CCTANATGTACTGCCAAGTAGGAAAGTCCCGTTAGGTCATGTACTGGGCATATGCCCAGC; GGCCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_002157
• Insert Size: 630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002157.1, NP_002148.1
• RefSeq Size: 538 bp
• RefSeq ORF: 309 bp
• Locus ID: 3336
• UniProt ID: P61604
• Cytogenetics: 2q33.1
• Domains: cpn10
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Summary: This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011]