Cpn10 (HSPE1) (NM_002157) Human Untagged Clone

CAT#: SC118785

HSPE1 (untagged)-Human heat shock 10kDa protein 1 (chaperonin 10) (HSPE1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cpn10
• Synonyms: CPN10; EPF; GROES; HSP10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002157 edited
  ATGGCAGGACAAGCGTTTAGAAAGTTTCTTCCACTCTTTGACCGAGTATTGGTTGAAAGG
  AGTGCTGCTGAAACTGTAACCAAAGGAGGCATTATGCTTCCAGAAAAATCTCAAGGAAAA
  GTATTGCAAGCAACAGTAGTCGCTGTTGGATCGGGTTCTAAAGGAAAGGGTGGAGAGATT
  CAACCAGTTAGCGTGAAAGTTGGAGATAAAGTTCTTCTCCCAGAATATGGAGGCACCAAA
  GTAGTTCTAGATGACAAGGATTATTTCCTATTTAGAGATGGTGACATTCTTGGAAAGTAC
  GTAGACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002157 unedited
  ATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGTGCGGCGCTACACTAGAGCAGA
  GTACGAGTCTGAGGCGGAGGGAGTAATGGCAGGACAAGCGTTTAGAAAGTTTCTTCCACT
  CTTTGACCGAGTATTGGTTGAAAGGAGTGCTGCTGAAACTGTAACCAAAGGAGGCATTAT
  GCTTCCAGAAAAATCTCAAGGAAAAGTATTGCAAGCAACAGTAGTCGCTGTTGGATCGGG
  TTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGGAGATAAAGTTCT
  TCTCCCAGAATATGGAGGCACCAAAGTAGTTCTAGATGACAAGGATTATTTCCTATTTAG
  AGATGGTGACATTCTTGGAAAGTACGTAGACTGAAATAAGTCACTATTGAAATGGCATCA
  ACATGATGCTGCCCATTCCACTGAAGTTCTGAAATCTTTCGTCATGTAAATAATTTCCAT
  ATTTCTCTTTTATAATAAACTAATGATAACTAATGACAAANAAAAAAAAAAAAAAAAAAA
  NNCCTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGC
  ATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGNAAGTTGCCACTCCAGTGCCCA
  CCAGCCTTGTCCTAAAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAT
  ATTATGGGGTGAGGGNGGTGGGTATGGAGCAGGGTGCNNAGTGGGAAAACACCTGTAGGN
  CCCTGCGGGTCTATTGGGAACAAGCTGAAGTGCAGTGCACAATCTTGGCTCACTGCATCT
  CCGCTNCTGGGTTCAGCGATTCTCCTGCTCAGCCTCCGGATTGTTGGGATTCCAGNCTGC
  ATGACCAGCTCACTAATTTTGTTTTTTGTAGAAACGGGTTTACCATATGGCCAGCTGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002157 unedited
  NNTTTCGCTCTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT
  TTTTTGGCATTAGTTATCATTAGTTTATTATAAAAGAAAAATTGGAAATTATTTACATGA
  CGAAAGATTTCAGAACTTCAGTGGAATGGGCAGCATCATGTTGATGCCATTTCAATAGTG
  ACTTATTTCAGTCTACGTACTTTCCAAGAATGTCACCATCTCTAAATAGGAAATAATCCT
  TGTCATCTAGAACTACTTTGGTGCCTCCATATTCTGGGAGAAGAACTTTATCTCCAACTT
  TCACGCTAACTGGTTGAATCTCTCCACCCTTTCCTTTAGAACCCGATCCAACAGCGACTA
  CTGGTGCTTGCAATACTTTTCCTTGAGATTTTTCTGGAAGCATAATGCCTCCTTTGGTTA
  CAGTTTCAGCAGCACTCCTTTCAACCAATACTCGGTCAAAGAGTGGAAGAAACTTTCTAA
  ACGCTTGTCCTGCCATTACTCCCTCCGCCTCAGACTCGTACTCTGCTCTAGTGTAGCGCC
  GCACCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGT
  TCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCG
  TCAACGGGGCGCGNGTTATTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAAC
  AAACTCCCATTGACGTCAATGGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTA
  TCCACGCCCATTTGGTGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATA
  CCTANATGTACTGCCAAGTAGGAAAGTCCCGTTAGGTCATGTACTGGGCATATGCCCAGC
  GGCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002157
• Insert Size: 630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002157.1, NP_002148.1
• RefSeq Size: 538 bp
• RefSeq ORF: 309 bp
• Locus ID: 3336
• UniProt ID: P61604
• Cytogenetics: 2q33.1
• Domains: cpn10
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Gene Summary: This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011]