Cytokeratin 19 (KRT19) (NM_002276) Human Untagged Clone

CAT#: SC118729

KRT19 (untagged)-Human keratin 19 (KRT19)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cytokeratin 19
• Synonyms: CK19; K1CS; K19
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118729 sequence for NM_002276 edited (data generated by NextGen Sequencing)
  ATGACTTCCTACAGCTATCGCCAGTCGTCGGCCACGTCGTCCTTCGGAGGCCTGGGCGGC
  GGCTCCGTGCGTTTTGGGCCGGGGGTCGCTTTTCGCGCGCCCAGCATTCACGGGGGCTCC
  GGCGGCCGCGGCGTATCCGTGTCCTCCGCCCGCTTTGTGTCCTCGTCCTCCTCGGGGGGC
  TACGGCGGCGGCTACGGCGGCGTCCTGACCGCGTCCGACGGGCTGCTGGCGGGCAACGAG
  AAGCTAACCATGCAGAACCTCAACGACCGCCTGGCCTCCTACCTGGACAAGGTGCGCGCC
  CTGGAGGCGGCCAACGGCGAGCTAGAGGTGAAGATCCGCGACTGGTACCAGAAGCAGGGG
  CCTGGGCCCTCCCGCGACTACAGCCACTACTACACGACCATCCAGGACCTGCGGGACAAG
  ATTCTTGGTGCCACCATTGAGAACTCCAGGATTGTCCTGCAGATCGACAACGCCCGTCTG
  GCTGCAGATGACTTCCGAACCAAGTTTGAGACGGAACAGGCTCTGCGCATGAGCGTGGAG
  GCCGACATCAACGGCCTGCGCAGGGTGCTGGATGAGCTGACCCTGGCCAGGACCGACCTG
  GAGATGCAGATCGAAGGCCTGAAGGAAGAGCTGGCCTACCTGAAGAAGAACCATGAGGAG
  GAAATCAGTACGCTGAGGGGCCAAGTGGGAGGCCAGGTCAGTGTGGAGGTGGATTCCGCT
  CCGGGCACCGATCTCGCCAAGATCCTGAGTGACATGCGAAGCCAATATGAGGTCATGGCC
  GAGCAGAACCGGAAGGATGCTGAAGCCTGGTTCACCAGCCGGACTGAAGAATTGAACCGG
  GAGGTCGCTGGCCACACGGAGCAGCTCCAGATGAGCAGGTCCGAGGTTACTGACCTGCGG
  CGCACCCTTCAGGGTCTTGAGATTGAGCTGCAGTCACAGCTGAGCATGAAAGCTGCCTTG
  GAAGACACACTGGCAGAAACGGAGGCGCGCTTTGGAGCCCAGCTGGCGCATATCCAGGCG
  CTGATCAGCGGTATTGAAGCCCAGCTGGGCGATGTGCGAGCTGATAGTGAGCGGCAGAAT
  CAGGAGTACCAGCGGCTCATGGACATCAAGTCGCGGCTGGAGCAGGAGATTGCCACCTAC
  CGCAGCCTGCTCGAGGGACAGGAAGATCACTACAACAATTTGTCTGCCTCCAAGGTCCTC
  TGA
  
  Clone variation with respect to NM_002276.4
  90 c=>t;179 c=>g;471 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002276 unedited
  GTTCANAATATGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGCAGCTTCT
  GAGACCAGGTTGCTCCGTCCGTGCTCCGCCTCGCCATGACTTCCTACAGCTATCGCCAGT
  CGTCGGCCACGTCGTCCTTCGGAGGCCTGGGCGGCGGCTCCGTGCGTTTTGGGCCGGGGG
  TCGCTTTTCGCGCGCCCAGCATTCACGGGGGCTCCGGCGGCCGCGGCGTATCCGTGTCCT
  CCGCCCGCTTTGTGTCCTCGTCCTCCTCGGGGGGCTACGGCGGCGGCTACGGCGGCGTCC
  TGACCGCGTCCGACGGGCTGCTGGCGGGCAACGAGAAGCTAACCATGCAGAACCTCAACG
  ACCGCCTGGCCTCCTACCTGGACAAGGTGCGCGCCCTGGAGGCGGCCAACGGCGAGCTAG
  AGGTGAAGATCCGCGACTGGTACCAGAAGCAGGGGCCTGGGCCCTCCCGCGACTACAGCC
  ACTACTACACGACCATCCAGGACCTGCGGGACAAGATTCTTGGTGCCACCATTGAGAACT
  CCAGGATTGTCCTGCAGATCGACAACGCCCGTCTGGCTGCAGATGACTTCCGAACCAAGT
  TTGAGACGGAACAGGCTCTGCGCATGAGCGTGGAGGCCGACATCAACGGCCTGCGCAGGG
  TGCTGGATGAGCTGACCCTGGCCAGGACCGACCTGGAGATGCAGATCGAAGGCCTGAAGG
  AAGAGCTGGCCTACCTGAAGAAGACCATGAGGAGGAAACAGTACGCTGAGGGGCCAAGTG
  GGAAGCCAGGTCAGTGTGGAGGTGGATTCCGCTCCGGGCACCGATCTCGCCCAGATCCTG
  AGTGACATGCGAAGCCAATATGATGTCATGGCCGAGCAAAACCGCAAGATGCTGAAGCCT
  GGTTTCACAGCCGGACTGTAAAATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002276 unedited
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTTGGACCATAAAT
  TTTTATTGGCAGGTCAGGAGAAGAGCCGGGGGTAAGGGTCCCTTCCTTCCCATCCCTCTA
  CCCAGAAGACACCCTCCAAAGGACAGCAGAAGCCCCAGAGCCTGCTGCCTCAGAGGACCT
  TGGAGGCAGACAAATTGTTGTAGTGATCTTCCTGTCCCTCGAGCAGGCTGCGGTAGGTGG
  CAATCTCCTGCTCCAGCCGCGACTTGATGTCCATGAGCCGCTGGTACTCCTGATTCTGCC
  GCTCACTATCAGCTCGCACATCGCCCAGCTGGGCTTCAATACCGCTGATCAGCGCCTGGA
  TATGCGCCAGCTGGGCTCCAAAGCGCGCCTCCGTTTCTGCCAGTGTGTCTTCCAAGGCAG
  CTTTCATGCTCAGCTGTGACTGCAGCTCAATCTCAAGACCCTGAAGGGTGCGCCGCAGGT
  CAGTAACCTCGGACCTGCTCATCTGGAGCTGCTCCGTGTGGCCAGCGACCTCCCGGTTCA
  ATTCTTCAGTCCGGCTGGTGAACCAGGCTTCAGCATCCTTCCGGTTCTGCTCGGCCATGA
  CCTCATATTGGCTTCGCATGTCACTCAGGATCTTGGCGAGATCGGTGCCCGGAGCGGAAA
  TCACCTCCACACTGACCTGGCCTCCCACTTGGCCCCTCAGCGTACTGATTTCCTCCTTAT
  GGTTCTTTTTTAGGTAAGCCATCTCTTCCTTTAGGGCCTTCGATCTGGATTCTCAGGTTG
  GGCCCTTGGCAGAGTCTAGTCTATCCACACCCTCGCCACGTCGGTGATGTTCCGCCTCCA
  CCTTATGCCACAAGACTGTTCTCGTTTAAACTGTGTCGGAAAGTCATTTCCGCCATAACG
  CGCGTTGTCTTCTCCGGAAACACCTGTAGCTCTCTAGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002276
• Insert Size: 1270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002276.3, NP_002267.2
• RefSeq Size: 1513 bp
• RefSeq ORF: 1203 bp
• Locus ID: 3880
• UniProt ID: P08727
• Cytogenetics: 17q21.2
• Domains: filament
• Gene Summary: The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]