KPNB1 (NM_002265) Human Untagged Clone

CAT#: SC118719

KPNB1 (untagged)-Human karyopherin (importin) beta 1 (KPNB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KPNB1
• Synonyms: IMB1; Impnb; IPO1; IPOB; NTF97
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002265 edited
  CCAACCCCCATCCCCAGTTCGAGCCGCCGCCCGAAAGGCCGGGCCGTCGTCTTAGGAGGA
  GTCGCCGCCGCCGCCACCTCCGCCATGGAGCTGATCACCATTCTCGAGAAGACCGTGTCT
  CCCGATCGGCTGGAGCTGGAAGCGGCGCAGAAGTTCCTGGAGCGTGCGGCCGTGGAGAAC
  CTGCCCACTTTCCTTGTGGAACTGTCCAGAGTGCTGGCAAATCCAGGAAACAGTCAGGTT
  GCCAGAGTTGCAGCTGGTCTACAAATCAAGAACTCTTTGACATCTAAAGATCCAGATATC
  AAGGCACAATATCAGCAGAGGTGGCTTGCTATTGATGCTAATGCTCGACGAGAAGTCAAG
  AACTATGTTTTGCAGACATTGGGTACAGAAACTTACCGGCCTAGTTCTGCCTCACAGTGT
  GTGGCTGGTATTGCTTGTGCAGAGATCCCAGTAAACCAGTGGCCAGAACTCATTCCTCAG
  CTGGTGGCCAATGTCACAAACCCCAACAGCACAGAGCACATGAAGGAGTCGACATTGGAA
  GCCATCGGTTATATTTGCCAAGATATAGACCCAGAGCAGCTACAAGATAAATCCAATGAG
  ATTCTGACTGCCATAATCCAGGGGATGAGGAAAGAAGAGCCTAGTAATAATGTGAAGCTA
  GCTGCTACGAATGCACTCCTGAACTCATTGGAGTTCACCAAAGCAAACTTTGATAAAGAG
  TCTGAAAGGCACTTTATTATGCAGGTGGTCTGTGAAGCCACACAGTGTCCAGATACGAGG
  GTACGAGTGGCTGCTTTACAGAATCTGGTGAAGATAATGTCCTTATATTATCAGTACATG
  GAGACATATATGGGTCCTGCTCTTTTTGCAATCACAATCGAAGCAATGAAAAGTGACATT
  GATGAGGTGGCTTTACAAGGGATAGAATTCTGGTCCAATGTCTGTGATGAGGAAATGGAT
  TTGGCCATTGAAGCTTCAGAGGCAGCAGAACAAGGACGGCCCCCTGAGCACACCAGCAAG
  TTTTATGCGAAGGGAGCACTACAGTATCTGGTTCCAATCCTCACACAGACACTAACTAAA
  CAGGACGAAAATGATGATGACGATGACTGGAACCCCTGCAAAGCAGCAGGGGTGTGCCTC
  ATGCTTCTGGCCACCTGCTGTGAAGATGACATTGTCCCACATGTCCTCCCCTTCATTAAA
  GAACACATCAAGAACCCAGATTGGCGGTACCGGGATGCAGCAGTGATGGCTTTTGGTTGT
  ATCTTGGAAGGACCAGAGCCCAGTCAGCTCAAACCACTAGTTATACAGGCTATGCCCACC
  CTAATAGAATTAATGAAAGACCCCAGTGTAGTTGTTCGAGATACAGCTGCATGGACTGTA
  GGCAGAATTTGTGAGCTGCTTCCTGAAGCTGCCATCAATGATGTCTACTTGGCTCCCCTG
  CTACAGTGTCTGATTGAGGGTCTCAGTGCTGAACCCAGAGTGGCTTCAAATGTGTGCTGG
  GCTTTCTCCAGTCTGGCTGAAGCTGCTTATGAAGCTGCAGACGTTGCTGATGATCAGGAA
  GAACCAGCTACTTACTGCTTATCTTCTTCATTTGAACTCATAGTTCAGAAGCTCCTAGAG
  ACTACAGACAGACCTGATGGACACCAGAACAACCTGAGGAGTTCTGCATATGAATCTCTG
  ATGGAAATTGTGAAAAACAGTGCCAAGGATTGTTATCCTGCTGTCCAGAAAACGACTTTG
  GTCATCATGGAACGACTGCAACAGGTTCTTCAGATGGAGTCACATATCCAGAGCACATCC
  GATAGAATCCAGTTCAATGACCTTCAGTCTTTACTCTGTGCAACTCTTCAGAATGTTCTT
  CGGAAAGTGCAACATCAAGATGCTTTGCAGATCTCTGATGTGGTTATGGCCTCCCTGTTA
  AGGATGTTCCAAAGCACAGCTGGGTCTGGGGGAGTACAAGAGGATGCCCTGATGGCAGTT
  AGCACACTGGTGGAAGTGTTGGGTGGTGAATTCCTCAAGTACATGGAGGCCTTTAAACCC
  TTCCTGGGCATTGGATTAAAAAATTATGCTGAATACCAGGTTTGTTTGGCAGCTGTGGGC
  TTAGTGGGAGACTTGTGCCGTGCCCTGCAATCCAACATCATACCTTTCTGTGACGAGGTG
  ATGCAGCTGCTTCTGGAAAATTTGGGGAATGAGAACGTCCACAGGTCTGTGAAGCCGCAG
  ATTCTGTCAGTGTTTGGTGATATTGCCCTTGCTATTGGAGGAGAGTTTAAAAAATACTTA
  GAGGTTGTATTGAATACTCTTCAGCAGGCCTCCCAAGCCCAGGTGGACAAGTCAGACTAT
  GACATGGTGGATTATCTGAATGAGCTAAGGGAAAGCTGCTTGGAAGCCTATACTGGAATC
  GTCCAGGGATTAAAGGGGGATCAGGAGAACGTACACCCGGATGTGATGCTGGTACAACCC
  AGAGTAGAATTTATTCTGTCTTTCATTGACCACATTGCTGGAGATGAGGATCACACAGAT
  GGAGTAGTAGCTTGTGCTGCTGGACTAATAGGGGACTTATGTACAGCATTTGGGAAGGAT
  GTACTGAAATTAGTAGAAGCTAGGCCAATGATCCATGAATTGTTAACTGAAGGGCGGAGA
  TCGAAGACTAACAAAGCAAAAACCCTTGCTACATGGGCAACAAAAGAACTGAGGAAACTG
  AAGAACCAAGCTTGATCTGTTACCATTGGGATGATAACCTGAGGACCCCCACTGGAAATC
  TCCCATCTTTTGAAAAACCTGGAAGTGAGGAGTGTGCACGGATGCTGAATGTTTGGGAAT
  GAGAGGATGAGTGAGTGAGGCTTGAAAACACACCACATTGAAAATCCTGCCACAGCAGCA
  GCCGCAGCCGCCAACAGCAGCGCTGTTAGTGAGCTAAGTAAGCACTGACTTCGTAGAAAA
  CCATAACATCGGCCATCTTGGAAAAGAGAAAAACAATGGAGTTACTTATTTAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002265 unedited
  GGGCACTATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCCAACCCCC
  ATCCCCAGTTCGAGCCGCCGCCCGAAGGCCGGGCCGTCGTCTTAGGAGGAGTCGCCGCCG
  CCGCCACCTCCGCCATGGAGCTGATCACCATTCTCGAGAAGACCGTGTCTCCCGATCGGC
  TGGAGCTGGAAGCGGCGCAGAAGTTCCTGGAGCGTGCGGCCGTGGAGAACCTGCCCACTT
  TCCTTGTGGAACTGTCCAGAGTGCTGGCAAATCCAGGAAACAGTCAGGTTGCCAGAGTTG
  CAGCTGGTCTACAAATCAAGAACTCTTTGACATCTAAAGATCCAGATATCAAGGCACAAT
  ATCAGCAGAGGTGGCTTGCTATTGATGCTAATGCTCGACGAGAAGTCAAGAACTATGTTT
  TGCAGACATTGGGTACAGAAACTTACCGGCCTAGTTCTGCCTCACAGTGTGTGGCTGGTA
  TTGCTTGTGCAGAGATCCCAGTAAACCAGTGGCCAGAACTCATTCCTCAGCTGGTGGCCA
  ATGTCACAAACCCCAACAGCACAGAGCACATGAAGGAGTCGACATTGGAAGCCATCGGTT
  ATATTTGCCAAGATATAGACCCAGAGCAGCTACAAGATAAATCCAATGAGATTCTGACTG
  CCATAATCCAGGGGATGAGGAAAGAAGAGCCTAGTAATAATGTGAAGCTAGCTGCTACGA
  ATGCACTCCTGAACTCATTGGAGTTCACCAAAGCANACTTTGNATAAGAGTCTGAAAGGC
  ACTNTATTATGCAGGTGGGTCTGTGAAGCCACACAGTGTCCAGATACGAGGTACGAGTGG
  GCTGCTTACAGAATCTGGTGAAGNATATGTCCTTATATTATCAGTACATGGAGACATATA
  TGGGTCCTGCTCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002265 unedited
  CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAATAAGTAACTCCATTGTTTTTC
  TCTTTTCCAAGATGGCCGATGTTATGGTTTTCTACGAAGTCAGTGCTTACTTATCTCACT
  AACAGCGCTGCTGTTGGCGGCTGCGGCTGCTGCTGTGGCAGGATTTTCAATGTGGTGTGT
  TTTCAAGCCTCACTCACTCATCCTCTCATTCCCAAACATTCAGCATCCGTGCACACTCCT
  CACTTCCAGGTTTTTCAAAAGATGGGAGATTTCCAGTGGGGGTCCTCAGGTTATCATCCC
  AATGGTAACAGATCAAGCTTGGTTCTTCAGTTTCCTCAGTTCTTTTGTTGCCCATGTAGC
  AAGGGTTTTTGCTTTGTTAGTCTTCGATCTCCGCCCTTCAGTTAACAATTCATGGATCAT
  TGGCCTAGCTTCTACTAATTTCAGTACATCCTTCCCAAATGCTGTACATAAGTCCCCTAT
  TAGTCCAGCAGCACAAGCTACTACTCCATCTGTGTGATCCTCATCTCCAGCAATGTGGTC
  AATGAAAGACAGAATAAATTCTACTCTGGGTTGTACCAGCATCACATCCGGGTGTACGTT
  CTCCTGATCCCCCTTTAATCCCTGGACGATTCCAGTATATGCCTCCAAGCAGCTNTCCCT
  TAGCTCATTCAGATAATCCACCATGTCATAGTCTGACTTGTCCACCTGGGCTAGGGAAGC
  CCGCTGAAAATTATTCAATACAACCCTCTAGTATTCTTTAACTCTCCTCAATAGCAAGGG
  CATCTCACCAAACCCTGACAGAATCTGCGGCTTCACAGACCTGTGGACGTTCTCATTCCC
  CAATTTTCCAAAGCCACTGCTCACCCCTGCCAAAAAGGTTAATGTTGATCGCAAGCCACG
  CCCAATCTTCCACTAACCCCAGCTTGCAAACAAACCGGTATTCAATATATTTTTATTCCA
  TGCCACGAAGGGTTAAGACTCCCTGACTCGAGAATTCCCCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002265
• Insert Size: 3180 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002265.4, NP_002256.2
• RefSeq Size: 4205 bp
• RefSeq ORF: 2631 bp
• Locus ID: 3837
• UniProt ID: Q14974
• Cytogenetics: 17q21.32
• Domains: Armadillo_seg, IBN_NT
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Gene Summary: Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.