galectin 9 (LGALS9) (NM_002308) Human Untagged Clone

CAT#: SC118707

LGALS9 (untagged)-Human lectin, galactoside-binding, soluble, 9 (LGALS9), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: galectin 9
• Synonyms: HUAT; LGALS9A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002308 edited
  GAATTCGGCACGAGGGAAAGGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAG
  CAGTTCCCAGGCTCCCTACCTGAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGG
  TCTCCAGGACGGACTTCAGATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAG
  GTTTGCTGTGAACTTTCAGACTGGCTTCAGTGGAAATGACATTGCCTTCCACTTCAACCC
  TCGGTTTGAAGATGGAGGGTACGTGGTGTGCAACACGAGGCAGAACGGAAGCTGGGGGCC
  CGAGGAGAGGAAGACACACATGCCTTTCCAGAAGGGGATGCCCTTTGACCTCTGCTTCCT
  GGTGCAGAGCTCAGATTTCAAGGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCA
  CCGCGTGCCCTTCCACCGTGTGGACACCATCTCCGTCAATGGCTCTGTGCAGCTGTCCTA
  CATCAGCTTCCAGCCTCCCGGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGT
  CATCCACACAGTGCAGAGCGCCCCTGGACAGATGTTCTCTACTCCCGCCATCCCACCTAT
  GATGTACCCCCACCCCGCCTATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTA
  CCCATCCAAGTCCATCCTCCTGTCAGGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACAT
  CAACCTGTGCTCTGGGAACCACATCGCCTTCCACCTGAACCCCCGTTTTGATGAGAATGC
  TGTGGTCCGCAACACCCAGATCGACAACTCCTGGGGGTCTGAGGAGCGAAGTCTGCCCCG
  AAAAATGCCCTTCGTCCGTGGCCAGAGCTTCTCAGTGTGGATCTTGTGTGAAGCTCACTG
  CCTCAAGGTGGCCGTGGATGGTCAGCACCTGTTTGAATACTACCATCGCCTGAGGAACCT
  GCCCACCATCAACAGACTGGAAGTGGGGGGCGACATCCAGCTGACCCATGTGCAGACATA
  GGCGGCTTCCTGGCCCTGGGGCCGGGGGCTGGGGTGTGGGGCAGTCTGGGTCCTCTCATC
  ATCCCCACTTCCCAGGCCCAGCCTTTCCAACCCTGCCTGGGATCTGGGCTTTAATGCAGA
  GGCCATGTCCTTGTCTGGTCCTGCTTCTGGCTACAGCCACCCTGGAACGGAGAAGGCAGC
  TGACGGGGATTGCCTTCCTCAGCCGCAGCAGCACCTGGGGCTCCAGCTGCTGGAATCCTA
  CCATCCCAGGAGGCAGGCACAGCCAGGGAGAGGGGAGGAGTGGGCAGTGAAGATGAAGCC
  CCATGCTCAGTCCCCTCCCATCCCCCACGCAGCTCCACCCCAGTCCCAAGCCACCAGCTG
  TCTGCTCCTGGTGGGAGGTGGCCTCCTCAGCCCCTCCTCTCTGACCTTTAACCTCACTCT
  CACCTTGCACCGTGCACCAACCCTTCACCCCTCCTGGAAAGCAGGCCTGATGGCTTCCCA
  CTGGCCTCCACCACCTGACCAGAGTGTTCTCTTCAGGGGACTGGCTCCTTTCCCAGTGTC
  CTTAAAATAAAGAAATGAAAATGCTTGTTGGCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002308 unedited
  GGTTCANATTTTGTAATACGACTTACTATAGGGCGGCCGCGCAATTCGCACGAGGGNAAA
  GGCAGCGGTGGCCACAGAGGCGGCGGAGAGATGGCCTTCAGCAGTTCCCAGGCTCCCTAC
  CTGAGTCCAGCTGTCCCCTTTTCTGGGACTATTCAAGGAGGTCTCCAGGACGGACTTCAG
  ATCACTGTCAATGGGACCGTTCTCAGCTCCAGTGGAACCAGGTTTGCTGTGAACTTTCAG
  ACTGGCTTCAGTGGAAATGACATTGCCTTCCACTTCAACCCTCGGTTTGAAGATGGAGGG
  TACGTGGTGTGCAACACGAGGCAGAACGGAAGCTGGGGGCCCGAGGAGAGGAAGACACAC
  ATGCCTTTCCAGAAGGGGATGCCCTTTGACCTCTGCTTCCTGGTGCAGAGCTCAGATTTC
  AAGGTGATGGTGAACGGGATCCTCTTCGTGCAGTACTTCCACCGCGTGCCCTTCCACCGT
  GTGGACACCATCTCCGTCAATGGCTCTGTGCAGCTGTCCTACATCAGCTTCCAGCCTCCC
  GGCGTGTGGCCTGCCAACCCGGCTCCCATTACCCAGACAGTCATCCACACAGTGCAGAGC
  GCCCCTGGACAGATGTTCTCTACTCCCGCCATCCCACCTATGATGTACCCCCACCCCGCC
  TATCCGATGCCTTTCATCACCACCATTCTGGGAGGGCTGTACCCATCCAAGTCCATCCTC
  CTGTCANGCACTGTCCTGCCCAGTGCTCAGAGGTTCCACATAAACCTGTGCTCTGGGAAC
  CACATNGCCTTNCACCTGNACCCCCGTTTTGATGAAGATGCTGTTGTCCCGCACACCCAG
  ATCGACAACTCCTGGGGGTCCTGAAGAACGAAGTCTGCCCCGAAAATGCCCTTCGTCCGT
  GGCCAGACTTCTCAGTGTGGATCTTTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002308 unedited
  TGACCGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGCCAACAAGCATTTT
  CATTTCTTTATTTTAAGGACACTGGNGAAAGGAGCCAGTCCCCTGAAGAGAACACTCTGG
  TCAGGTGGTGGAGGCCAGTGGGAAGCCATCAGGCCTGCTTTCCAGGAGGGGTGAAGGGTT
  GGTGCACGGTGCAAGGTGAGAGTGAGGTTAAAGGTCAGAGAGGAGGGGCTGAGGAGGCCA
  CCTCCCACCAGGAGCAGACAGCTGGTGGCTTGGGACTGGGGTGGAGCTGCGTGGGGGATG
  GGAGGGGACTGAGCATGGGGCTTCATCTTCACTGCCCACTCCTCCCCTCTCCCTGGCTGT
  GCCTGCCTCCTGGGATGGTAGGATTCCAGCAGCTGGAGCCCCAGGTGCTGCTGCGGCTGA
  GGAAGGCAATCCCCGTCAGCTGCCTTCTCCGTTCCAGGGTGGCTGTAGCCAGAAGCAGGA
  CCAGACAAGGACATGGCCTCTGCATTAAAGCCCAGATCCCAGGCAGGGTTGGAAAGGCTG
  GGCCTGGGAAGTGGGGATGATGAGAGGACCCANACTGCCCCACACCCCAGCCCCCGGCCC
  CAGGGCCAGGAAGCCGCCTATGTCTGCACATGGGTCAGCTGGATGTCGCCCCCCACTTCC
  AGTCTGTTGATGGTGGGCAGGTTCCTCAGGCGATGGTAGTATTCAAACAGGTGCTGACCA
  TCCACGGCCACCTTNGAGCAGTGAGCTTCACACAAGATCCACACTGAGAAGCTCTGGCCA
  CGGACGAAGGGCATTTTTTCGGGGCAGACTTCGCTTCTCAGACCCCCCAGNAGTGTCGAT
  CTTGGTGTTGCCGACCACAGCATTCTCATCAAAACGGGGGTTTCAGGTGGAAGGCGATGT
  TGTTCCCACACN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002308
• Insert Size: 1700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002308.2, NP_002299.1
• RefSeq Size: 1620 bp
• RefSeq ORF: 972 bp
• Locus ID: 3965
• UniProt ID: O00182
• Cytogenetics: 17q11.2
• Domains: Gal-bind_lectin
• Gene Summary: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) is lacking an internal, in-frame coding exon compared to variant 1, resulting in a shorter isoform (short) missing a 32 aa protein segment compared to isoform long.