MARK3 (NM_002376) Human Untagged Clone

CAT#: SC118703

MARK3 (untagged)-Human MAP/microtubule affinity-regulating kinase 3 (MARK3), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARK3
• Synonyms: CTAK1; KP78; Par-1a; PAR1A; VIPB
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002376 edited
  ATGTCCACTAGGACCCCATTGCCAACGGTGAATGAACGAGACACTGAAAACCACACGTCA
  CATGGAGATGGGCGTCAAGAAGTTACCTCTCGTACCAGCCGCTCAGGAGCTCGGTGTAGA
  AACTCTATAGCCTCCTGTGCAGATGAACAACCTCACATCGGAAACTACAGACTGTTGAAA
  ACAATCGGCAAGGGGAATTTTGCAAAAGTAAAATTGGCAAGACATATCCTTACAGGCAGA
  GAGGTTGCAATAAAAATAATTGACAAAACTCAGTTGAATCCAACAAGTCTACAAAAGCTC
  TTCAGAGAAGTAAGAATAATGAAGATTTTAAATCATCCCAATATAGTGAAGTTATTCGAA
  GTCATTGAAACTGAAAAAACACTCTACCTAATCATGGAATATGCAAGTGGAGGTGAAGTA
  TTTGACTATTTGGTTGCACATGGCAGGATGAAGGAAAAAGAAGCAAGATCTAAATTTAGA
  CAGATTGTGTCTGCAGTTCAATACTGCCATCAGAAACGGATCGTACATCGAGACCTCAAG
  GCTGAAAATCTATTGTTAGATGCCGATATGAACATTAAAATAGCAGATTTCGGTTTTAGC
  AATGAATTTACTGTTGGCGGTAAACTCGACACGTTTTGTGGCAGTCCTCCATACGCAGCA
  CCTGAGCTCTTCCAGGGCAAGAAATATGACGGGCCAGAAGTGGATGTGTGGAGTCTGGGG
  GTCATTTTATACACACTAGTCAGTGGCTCACTTCCCTTTGATGGGCAAAACCTAAAGGAA
  CTGAGAGAGAGAGTATTAAGAGGGAAATACAGAATTCCCTTCTACATGTCTACAGACTGT
  GAAAACCTTCTCAAACGTTTCCTGGTGCTAAATCCAATTAAACGCGGCACTCTAGAGCAA
  ATCATGAAGGACAGGTGGATCAATGCAGGGCATGAAGAAGATGAACTCAAACCATTTGTT
  GAACCAGAGCTAGACATCTCAGACCAAAAAAGAATAGATATTATGGTGGGAATGGGATAT
  TCACAAGAAGAAATTCAAGAATCTCTTAGTAAGATGAAATACGATGAAATCACAGCTACA
  TATTTGTTATTGGGGAGAAAATCTTCAGAGCTGGATGCTAGTGATTCCAGTTCTAGCAGC
  AATCTTTCACTTGCTAAGGTTAGGCCGAGCAGTGATCTCAACAACAGTACTGGCCAGTCT
  CCTCACCACAAAGTGCAGAGAAGTGTTTCTTCAAGCCAAAAGCAAAGACGCTACAGTGAC
  CATGCTGGACCAGCTATTCCTTCTGTTGTGGCGTATCCGAAAAGGAGTCAGACCAGCACT
  GCAGATAGTGACCTCAAAGAAGATGGAATTTCCTCCCGGAAATCAAGTGGCAGTGCTGTT
  GGAGGAAAGGGAATTGCTCCAGCCAGTCCCATGCTTGGGAATGCAAGTAATCCTAATAAG
  GCGGATATTCCTGAACGCAAGAAAAGCTCCACTGTCCCTAGTAGTAACACAGCATCTGGT
  GGAATGACACGACGAAATACTTATGTTTGCAGTGAGAGAACTACAGCTGATAGACACTCA
  GTGATTCAGAATGGCAAAGAAAACAGCACTATTCCTGATCAGAGAACTCCAGTTGCTTCA
  ACACACAGTATCAGTAGTGCAGCCACCCCAGATCGAATCCGCTTCCCAAGAGGCACTGCC
  AGTCGTAGCACTTTCCACGGCCAGCCCCGGGAACGGCGAACCGCAACATATAATGGCCCT
  CCTGCCTCTCCCAGCCTGTCCCATGAAGCCACACCATTGTCCCAGACTCGAAGCCGAGGC
  TCCACTAATCTCTTTAGTAAATTAACTTCAAAACTCACAAGGAGTCGCAATGTATCTGCT
  GAGCAAAAAGATGAAAACAAAGAAGCAAAGCCTCGATCCCTACGCTTCACCTGGAGCATG
  AAAACCACTAGTTCAATGGATCCCGGGGACATGATGCGGGAAATCCGCAAAGTGTTGGAC
  GCCAATAACTGCGACTATGAGCAGAGGGAGCGCTTCTTGCTCTTCTGCGTCCACGGAGAT
  GGGCACGCGGAGAACCTCGTGCAGTGGGAAATGGAAGTGTGCAAGCTGCCAAGACTGTCT
  CTGAACGGGGTCCGGTTTAAGCGGATATCGGGGACATCCATAGCCTTCAAAAATATTGCT
  TCCAAAATTGCCAATGAGCTAAAGCTGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002376 unedited
  NTTTATAACCCCCGCCCGTTGGCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATA
  TAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGC
  CGCGAATTCGGCACCAGCCACGGCGCCTTTTCGGAACTGCCGTGGACTCGAGGACGCTGG
  TCGCCGGCCTCCTAGGGCTGTGCTGTTTTGTTTTGACCCTCGCATTGTGCAGAATTAAAG
  TGCAGTAAAATGTCCACTAGGACCCCATTGCCAACGGTGAATGAACGAGACACTGAAAAC
  CACACGTCACATGGAGATGGGCGTCAAGAAGTTACCTCTCGTACCAGCCGCTCAGGAGCT
  CGGTGTAGAAACTCTATAGCCTCCTGTGCAGATGAACAACCTCACATCGGAAACTACAGA
  CTGTTGAAAACAATCGGCAAGGGGAATTTTGCAAAAGTAAAATTGGCAAGACATATCCTT
  ACAGGCAGAGAGGTTGCAATAAAAATAATTGACAAAACTCAGTTGAATCCAACAAGTCTA
  CAAAAGCTCTTCAGAGAAGTAAGAATAATGAAGATTTTAAATCATCCCAATATAGTGAAG
  TTATTCGAAGTCATTGAAACTGAAAAAACACTCTACCTAATCATGGAATATGCAAGTGGA
  GGTGAAGTATTTGACTATTTGGTTGCACATGGCAGGATGAAGGAAAAAGAAGCAAGATCT
  AAATTTAGACAGATTGTGTCTGCAGTTCAATACTGCCATCAGAAACGGATCGTACATCGA
  GACCTCAAGGCTGANAATCTATTGTTAGATGCCGATATGAACATTAAAATAGCAGATTTC
  GGTTTTAGCAATGAATTTACTGTTGGCGGTAAACTCGACACGTTTTGTGGCAGTCCTCCC
  TA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002376 unedited
  AATGTGGCTCANACTTCCATTTATGATTTAGTAANAGCAGACGTTATTGCCTAATATTAT
  TCTATACATTTCCATCAGTGTTCAGGAAAACACTTTAATTGCTACTTAATTTACATCATA
  ATCACTGGGTTACAGCTTTAGCTCATTGGCAATTTTGGAAGCAATATTTTTGAAGGCTAT
  GGATGTCCCCGATATCCGCTTAAACCGGACCCCGTTCATATTCAGTCTTGGCAGCTTGCA
  CACTTCCATTTCCCACTGCACGAGGTTCTCCGCGTGCCCATCTCCGTGGACGCAGAAGAG
  CAAGAAGCGCTCCCTCTGCTCATAGTCGCAGTTATTGGCGTCCAACACTTTGCGGATTTC
  CCGCATCATGTCCCCGGGATCCATTGAACTAGTGGTTTTCATGCTCCAGGTGAAGCGTAG
  GGATCGAGGCTTTGCTTCTTTGTTTTCATCTTTTTGCTCAGCAGATACATTGCGACTCCT
  TGTGAGTTTTGAAGTTAATTTACTAAAGAGATTAGTGGAGCCTCGGCTTCGAGTCTGGGA
  CANTGGTGTGGCTTCATGGGACAGGCTGAGAGAGGCAGGAGGGCCATTATATGGTGCGGT
  TCGCCGTTCCCGGAGCTGGCCGTGGAAAGGCTACGAGTGGGATTGCCTTTTGAGAAGCGA
  ATCCAATCTGGGTGGGTGCACTACTGTAACTGAGTGATGAAGCGACTGGGATTCCACGAT
  CAGAAAAATGCTGGATACTTTGGCTGTCGGAATACGGGAGGGCGAAACCGTGTGGTTTAC
  CACAGGCAAGGAGAGATTGGGAGGGGGCAACACCATCTATGACTGGTTCCACTTCAAAGA
  TGGAATGTTTTTTAGCGGGAAGGGAGCGACCCGGACCGAGCCGTGGACTTCCCGGCTGGT
  CCGGCCCGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002376
• Insert Size: 3810 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002376.4, NP_002367.4
• RefSeq Size: 3404 bp
• RefSeq ORF: 2190 bp
• Locus ID: 4140
• UniProt ID: P27448
• Cytogenetics: 14q32.32-q32.33
• Domains: UBA, pkinase, TyrKc, KA1, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (3) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a.