MCM3 (NM_002388) Human Untagged Clone

CAT#: SC118658

MCM3 (untagged)-Human minichromosome maintenance complex component 3 (MCM3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCM3
• Synonyms: HCC5; P1-MCM3; P1.h; RLFB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002388, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTCCCAAGGTCCCCGCCTCTTCCTCGAGGTAACCTTTGGTGGCGGGAAGAGTTCGGAAGTTTTCGCG
  CCGGGGTGGAGTCATCCTGGGAACCTCCACGCGACTTTGGTGGAGGTAGTTCTTTGGCAGCGGGCATGGC
  GGGTACCGTGGTGCTGGACGATGTGGAGCTGCGGGAGGCTCAGAGAGATTACCTGGACTTCCTGGACGAC
  GAGGAAGACCAGGGAATTTATCAGAGCAAAGTTCGGGAGCTGATCAGTGACAACCAATACCGGCTGATTG
  TCAATGTGAATGACCTGCGCAGGAAAAACGAGAAGAGGGCTAACCGGCTTCTGAACAATGCCTTTGAGGA
  GCTGGTTGCCTTCCAGCGGGCCTTAAAGGATTTTGTGGCCTCCATTGATGCTACCTATGCCAAGCAGTAT
  GAGGAGTTCTACGTAGGACTGGAAGGCAGCTTTGGCTCCAAGCACGTCTCCCCGCGGACTCTTACCTCCT
  GCTTCCTCAGCTGTGTGGTCTGTGTGGAGGGCATTGTCACTAAATGTTCTCTAGTTCGTCCCAAAGTCGT
  CCGCAGTGTCCACTACTGTCCTGCTACTAAGAAGACCATAGAGCGACGTTATTCTGATCTCACCACCCTG
  GTGGCCTTTCCCTCCAGCTCTGTCTATCCTACCAAGGATGAGGAGAACAATCCCCTTGAGACAGAATATG
  GCCTTTCTGTCTACAAGGATCACCAGACCATCACCATCCAGGAGATGCCGGAGAAGGCCCCAGCCGGCCA
  GCTCCCCCGCTCTGTGGACGTCATTCTGGATGATGACTTGGTGGATAAAGCGAAGCCTGGTGACCGGGTT
  CAGGTGGTGGGAACCTACCGTTGCCTTCCTGGAAAGAAGGGAGGCTACACCTCTGGGACCTTCAGGACTG
  TCCTGATTGCCTGTAATGTTAAGCAGATGAGCAAGGATGCTCAGCCCTCTTTCTCTGCTGAGGATATAGC
  CAAGATCAAGAAGTTCAGTAAAACCCGATCCAAGGATATCTTTGACCAGCTGGCCAAGTCATTGGCCCCA
  AGTATCCATGGGCATGACTATGTCAAGAAAGCAATCCTCTGCTTGCTCTTGGGAGGGGTGGAACGAGACC
  TAGAAAATGGCAGCCACATCCGTGGGGACATCAATATTCTTCTAATAGGAGACCCATCCGTTGCCAAGTC
  TCAGCTTCTGCGGTATGTGCTTTGCACTGCACCCCGAGCTATCCCCACCACTGGCCGGGGCTCCTCTGGA
  GTGGGTCTGACGGCTGCTGTCACCACAGACCAGGAAACAGGAGAGCGCCGTCTGGAAGCAGGGGCCATGG
  TCCTGGCTGACCGAGGCGTGGTTTGCATTGATGAATTTGACAAAATGTCTGACATGGATCGCACAGCCAT
  CCATGAAGTGATGGAGCAGGGTCGAGTGACCATTGCCAAGGCTGGCATCCATGCTCGGCTGAATGCCCGC
  TGCAGTGTTTTGGCAGCTGCCAACCCTGTCTACGGCAGGTATGACCAGTATAAGACTCCAATGGAGAACA
  TTGGGCTACAGGACTCACTGCTGTCACGATTTGACTTGCTCTTCATCATGCTGGATCAGATGGATCCTGA
  GCAGGATCGGGAGATCTCAGACCATGTCCTTCGGATGCACCGTTACAGAGCACCTGGGGAGCAGGATGGC
  GATGCTATGCCCTTGGGTAGTGCTGTGGATATCCTGGCCACAGATGATCCCAACTTTAGCCAGGAAGATC
  AGCAGGACACCCAGATTTATGAGAAGCATGACAACCTTCTACATGGGACCAAGAAGAAAAAGGAGAAGAT
  GGTGAGTGCAGCATTCATGAAGAAGTACATCCATGTGGCCAAAATCATCAAGCCTGTCCTGACACAGGAG
  TCGGCCACCTACATTGCAGAAGAGTATTCACGCCTGCGCAGCCAGGATAGCATGAGCTCAGACACCGCCA
  GGACATCTCCAGTTACAGCCCGAACACTGGAAACTCTGATTCGACTGGCCACAGCCCATGCGAAGGCCCG
  CATGAGCAAGACTGTGGACCTGCAGGATGCAGAGGAAGCTGTGGAGTTGGTCCAGTATGCTTACTTTAAG
  AAGGTTCTGGAGAAGGAGAAGAAACGTAAGAAGCGAAGTGAGGATGAATCAGAGACAGAAGATGAAGAGG
  AGAAAAGCCAAGAGGACCAGGAGCAGAAGAGGAAGAGAAGGAAGACTCGCCAGCCAGATGCCAAAGATGG
  GGATTCATACGACCCCTATGACTTCAGTGACACAGAGGAGGAAATGCCTCAAGTACACACTCCAAAGACG
  GCAGACTCACAGGAGACCAAGGAATCCCAGAAAGTGGAGTTGAGTGAATCCAGGTTGAAGGCATTCAAGG
  TGGCCCTCTTGGATGTGTTCCGGGAAGCTCATGCGCAGTCAATCGGCATGAATCGCCTCACAGAATCCAT
  CAACCGGGACAGCGAAGAGCCCTTCTCTTCAGTTGAGATCCAGGCTGCTCTGAGCAAGATGCAGGATGAC
  AATCAGGTCATGGTGTCTGAGGGCATCATCTTCCTCATCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002388 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCACGCGACTTT
  GGTGGAGGTAGTTCTTTGGCAGCGGGCATGGCGGGTACCGTGGTGCTGGACGATGTGGAG
  CTGCGGGAGGCTCAGAGAGATTACCTGGACTTCCTGGACGACGAGGAAGACCAGGGAATT
  TATCAGAGCAAAGTTCGGGAGCTGATCAGTGACAACCAATACCGGCTGATTGTCAATGTG
  AATGACCTGCGCAGGAAAAACGAGAAGAGGGCTAACCGGCTTCTGAACAATGCCTTTGAG
  GAGCTGGTTGCCTTCCAGCGGGCCTTAAAGGATTTTGTGGCCTCCATTGATGCTACCTAT
  GCCAAGCAGTATGAGGAGTTCTACGTAGGACTGGAAGGCAGCTTTGGCTCCAAGCACGTC
  TCCCCGCGGACTCTTACCTCCTGCTTCCTCAGCTGTGTGGTCTGTGTGGAGGGCATTGTC
  ACTAAATGTTCTCTAGTTCGTCCCAAAGTCGTCCGCAGTGTCCACTACTGTCCTGCTACT
  AAGAAGACCATAGAGCGACGTTATTCTGATCTCACCACCCTGGTGGCCTTTCCCTCCAGC
  TCTGTCTATCCTACCAAGGATGAGGAGAACAATCCCCTTGAGACAGAATATGGCCTTTCT
  GTCTACAAGGATCACCAGACCATCACCATCCAGGAGATGCCGGAGAAGGGCCCCAGCCGG
  CCAGCTCCCCCGCTCTGTGGACGTCATTCTGGATGATGACTTGGTGGATAAGCGAACCTG
  GTGACCGGTTCAGGTGTGGGGAACTACCGGTGNCTTCTGGAAGAGGGAGGCTACACTNTG
  GGACTTTCAGACTGGCCTGATTGCTGTAATGGTACCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002388 unedited
  CTTGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTGTACGAAATGGC
  TAAGTTTATTCAACACTCTCGGAATTCATCTGGATATTGGGTTTGTTTTGTGATACAATA
  CATATTCACCTTAACTGGTGCTACTGCAAAGAAAGCTTTCTTGACCTGCATGACGTGCCT
  CAGAGCTTCTCTCCACCAATTGGAACCACCCAAAGCCTAGTCTAGACCAAAGTGCTCTGG
  AGAAAAAAAACAAAACAAAAAAACAGCAAACAGAAAACAGTTGTGCCCCCAAAAGTACTC
  AGAAGTCATATGTTATTTACAATTGGGTTTGTGTGGGATGGGAAGTAGGGCGGATGAGCC
  AGTGCTTTTGCAATGAAGATGCAATAGTCATTGTCCTCTCCCACTGTCTCCTCTTTCCTC
  ACCCCATGGCAGCTTTCATGACCCATTCCCAAAGGGTCCACCGAGTCCTGAACTCAGCTT
  CATCACCAACATTCCTCGCCTTCAGTTGAATTCAACACTGTTAAGGGAGTAGAGGCAAAG
  ACTTGGGTCAGGGAGAGGGTGGGAAACACAGAACAAACTCTCTCGGCACAACCCAAGTTC
  AGAGACGAGGCCTCCTCAGATGAGGAAGATGATGCCCTCAGACACCATGACCTGATTGTC
  ATCCTGCATCTTGCTCAGAGCAGCCTGGATCTCAACTGAAGAGAANGGGCTCTCGCTGTC
  CCGGNTGATGGGATCTGTGAGGCGATTCATGCCGATTGACTGCGCATGAGCTTCCCGGAA
  CACATNNCAGAGGGCACCTTGNATGCCNTCACCTGNATCACTCACTCCACTTTCTGGATN
  NCTGGNCTCTGTGATCTGCCGCTTTGGATGTGTACTGAGCATTNCTCCTTGNGTACTGAG
  TCTAAGGTCGATGATCCCATCTTGCATCTGCTGGAGGCTNCTTCCTTCTTTTGGTCTGCC
  CCTGGCTTTCTCTTAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002388
• Insert Size: 2840 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002388.3, NP_002379.2
• RefSeq Size: 3143 bp
• RefSeq ORF: 2427 bp
• Locus ID: 4172
• UniProt ID: P25205
• Cytogenetics: 6p12.2
• Domains: MCM, AAA
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Gene Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).