MYD88 (NM_002468) Human Untagged Clone

CAT#: SC118606

MYD88 (untagged)-Human myeloid differentiation primary response gene (88) (MYD88), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MYD88
• Synonyms: IMD68; MYD88D
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002468 edited
  GAATTCGGCACGAGGCGCGCTGAGGCTCCAGGACCGCCCGCCATGGCTGCAGGAGGTCCC
  GGCGCGGGGTCTGCGGCCCCGGTCTCCTCCACATCCTCCCTTCCCCTGGCTGCTCTCAAC
  ATGCGAGTGCGGCGCCGCCTGTCTCTGTTCTTGAACGTGCGGACACAGGTGGCGGCCGAC
  TGGACCGCGCTGGCGGAGGAGATGGACTTTGAGTACTTGGAGATCCGGCAACTGGAGACA
  CAAGCGGACCCCACTGGCAGGCTGCTGGACGCCTGGCAGGGACGCCCTGGCGCCTCTGTA
  GGCCGACTGCTCGAGCTGCTTACCAAGCTGGGCCGCGACGACGTGCTGCTGGAGCTGGGA
  CCCAGCATTGAGGAGGATTGCCAAAAGTATATCTTGAAGCAGCAGCAGGAGGAGGCTGAG
  AAGCCTTTACAGGTGGCCGCTGTAGACAGCAGTGTCCCACGGACAGCAGAGCTGGCGGGC
  ATCACCACACTTGATGACCCCCTGGGGCATATGCCTGAGCGTTTCGATGCCTTCATCTGC
  TATTGCCCCAGCGACATCCAGTTTGTGCAGGAGATGATCCGGCAACTGGAACAGACAAAC
  TATCGACTGAAGTTGTGTGTGTCTGACCGCGATGTCCTGCCTGGCACCTGTGTCTGGTCT
  ATTGCTAGTGAGCTCATCGAAAAGAGGTGCCGCCGGATGGTGGTGGTTGTCTCTGATGAT
  TACCTGCAGAGCAAGGAATGTGACTTCCAGACCAAATTTGCACTCAGCCTCTCTCCAGGT
  GCCCATCAGAAGCGACTGATCCCCATCAAGTACAAGGCAATGAAGAAAGAGTTCCCCAGC
  ATCCTGAGGTTCATCACTGTCTGCGACTACACCAACCCCTGCACCAAATCTTGGTTCTGG
  ACTCGCCTTGCCAAGGCCTTGTCCCTGCCCTGAAGACTGTTCTGAGGCCCTGGGTGTGTG
  TGTATCTGTCTGCCTGTCCATGTACTTCTGCCCTGCCTCCTCCTTTCGTTGTAGGAGGAA
  TCTGTGCTCTACTTACCTCTCAATTCCTGGAGATGCCAACTTCACAGACACGTCTGCAGC
  AGCTGGACATCACATTTCATGTCCTGCATGGAACCAGTGGCTGTGAGTGGCATGTCCACT
  TGCTGGATTATCAGCCAGGACACTATAGAACAGGACCAGCTGAGACTAAGAAGGACCAGC
  AGAGCCAGCTCAGCTCTGAGCCATTCACACATCTTCACCCTCAGTTTCCTCACTTGAGGA
  GTGGGATGGGGAGAACAGAGAGTAGCTGTGTTTGAATCCCTGTAGGAAATGGTGAAGCAT
  AGCTCTGGGTCTCCTGGGGGAGACCAGGCTTGGCTGCGGGAGAGCTGGCTGTTGCTGGAC
  TACATGCTGGCCACTGCTGTGACCACGACACTGCTGGGGCAGCTTCTTCCACAGTGATGC
  CTACTGATGCTTCAGTGCCTCTGCACACCGCCCATTCCACTTCCTCCTTCCCCACAGGGC
  AGGTGGGGAAGCAGTTTGGCCCAGCCCAAGGAGACCCCACCTTGAGCCTTATTTCCTAAT
  GGGTCCACCTCTCATCTGCATCTTTCACACCTCCCAGCTTCTGCCCAACCTTCAGCAGTG
  ACAAGTCCCCAAGAGACTCGCCTGAGCAGCTTGGGCTGCTTTTCATTTCCACCTGTCAGG
  ATGCCTGTGGTCATGCTCTCAGCTCCACCTGGCATGAGAAGGGATCCTGGCCTCTGGCAT
  ATTCATCAAGTATGAGTTCTGGGGATGAGTCACTGTAATGATGTGAGCAGGGAGCCTTCC
  TCCCTGGGCCACCTGCAGAGAGCTTTCCCACCAACTTTGTACCTTGATTGCCTTACAAAG
  TTATTTGTTTACAAACAGCGACCATATAAAAGCCTCCTGCCCCAAAGCTTGTGGGCACAT
  GGGCACATACAGACTCACATACAGACACACACATATATGTACAGACATGTACTCTCACAC
  ACACAGGCACCAGCATACACACGTTTTTCTAGGTACAGCTCCCAGGAACAGCTAGGTGGG
  AAAGTCCCATCACTGAGGGAGCCTAACCATGTCCCTGAACAAAAATTGGGCACTCATCTA
  TTCCTTTTCTCTTGTGTCCCTACTCATTGAAACCAAACTCTGGAAAGGACCCAATGTACC
  AGTATTTATACCTCTAGTGAAGCACAGAGAGAGGAAGAGAGCTGCTTAAACTCACACAAC
  AATGAACTGCAGACACAGCTGTTCTCTCCCTCTCTCCTTCCCAGAGCAATTTATACTTTA
  CCCTCAGGCTGTCCTCTGGGGAGAAGGTGCCATGGTCTTAGGTGTCTGTGCCCCAGGACA
  GACCCTAGGACCCTAAATCCAATAGAAAATGCATATCTTTGCTCCACTTTCAGCCAGGCT
  GGAGCAAGGTACCTTTTCTTAGGATCTTGGGAGGGAATGGATGCCCCTCTCTGCATGATC
  TTGTTGAGGCATTTAGCTGCCATGCACCTGTCCCCCTTTAATACTGGGCATTTTAAAGCC
  ATCTCAAGAGGCATCTTCTACATGTTTTGTACGCATTAAAATAATTTCAAAGATATCTGA
  GAAAAGCCGATATTTGCCATTCTTCCTATATCCTGGAATATATCTTGCATCCTGAGTTTA
  TAATAATAAATAATATTCTACCTTGGAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002468 unedited
  AATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCGCTGAGGCT
  CCAGGACCGCCCGCCATGGCTGCAGGAGGTCCCGGCGCGGGGTCTGCGGCCCCGGTCTCC
  TCCACATCCTCCCTTCCCCTGGCTGCTCTCAACATGCGAGTGCGGCGCCGCCTGTCTCTG
  TTCTTGAACGTGCGGACACAGGTGGCGGCCGACTGGACCGCGCTGGCGGAGGAGATGGAC
  TTTGAGTACTTGGAGATCCGGCAACTGGAGACACAAGCGGACCCCACTGGCAGGCTGCTG
  GACGCCTGGCAGGGACGCCCTGGCGCCTCTGTAGGCCGACTGCTCGAGCTGCTTACCAAG
  CTGGGCCGCGACGACGTGCTGCTGGAGCTGGGACCCAGCATTGAGGAGGATTGCCAAAAG
  TATATCTTGAAGCAGCAGCAGGAGGAGGCTGAGAAGCCTTTACAGGTGGCCGCTGTAGAC
  AGCAGTGTCCCACGGACAGCAGAGCTGGCGGGCATCACCACACTTGATGACCCCCTGGGG
  CATATGCCTGAGCGTTTCGATGCCTTCATCTGCTATTGCCCCAGCGACATCCAGTTTGTG
  CAGGAGATGATCCGGCAACTGGAACAGACAAACTATCGACTGAAGTTGTGTGTGTCTGAC
  CGCGATGTCCTGCCTGGCACCTGTGTCTGGTCTATTGCTAGTGAGCTCATCGAAAAGAGT
  GCCGCCGGATGGTGGTGGTTGTCTCTGATGATTACCTGCAGAGCAAGGAATGTGACTNCC
  AGACCAAATTGCACTCAGCCTCTCTTCAGGTGCCCATCAGAAGCGACTGATCCCCCATCA
  GTACCAGGGCATGAAGAAAGAGCTCCCCAGCATCCTCGAGGTCATCACTGTCTGCGACTA
  CACCAACCCCTGCACCAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002468 unedited
  ATGGCCGCGGCCGCATTCTAGATCGAGTTTTTTTTTTTTTTTTTTTTCCAGGTAGNAAAT
  TATTTATTATTATAAACTCAGGATGCAAGATATATTCCAGGATATAGGAAGAATGGCAAA
  TATCGGCTTTTCTCAAATATCTTTGAAATTATTTTAATGCGTACAAAACATGTAGAAAAT
  GCCTCTTGAGATGGCTTTAAAATGCCCAGTATTAAAGGGGGACAGGTGCATGGCAGCTAA
  ATGCCTCAACAAGATCATGCAGAGAGGGGCATCCATTCCCTCCCAAGATCCTAAGAAAAG
  GTACCTTGCTCCAGCCTGGCTGAAAGTGGAGCAAAGATATGCATTTTCTATTGGATTTAG
  GGTCCTAGGGTCTGTCCTGGGGCACAGACACCTAAGACCATGGCACCTTCTCCCCAGAGG
  ACAGCCTGAGGGTAAAGTATAAATTGCTCTGGGAAGGAGAGAGGGAGAGAACAGCTGTGT
  CTGCAGTTCATTGTTGTGTGAGTTTAAGCAGCTCTCTTCCTCTCTCTGTGCTTCACTAGA
  GGTATAAATACTGGTACATTGGGTCCTTTCCAGAGTTTGGTTTCAATGAGTAGGGACACA
  AGAGAAAAGGAATAGATGAGTGCCCAATTTTTGTTCAGGGACATGGTTAGGCTCCCTCAG
  TGATGGGACTTTCCCACCTAGCTGTTCCTGGGAGCTGTACCTAGAANAACGTGTGTATGC
  TGGTGCCTGTGTGTGTGAGAGTACATGTCTGTACATATATGTNGTGNGTCTGTATGTGAG
  TCTGTATGTGCCCATGTGCCCACAAGCTTTTGGGCANGAGGCTTTATATGGTCGCTGTTT
  GTAACAAATNACTTTGTAGGGCATCAGGTACAAAAGTTGTGGNNAGCTCTCTGCANGTGG
  CCAGGGAGAANGCCTCCTGCTCACATCATACGGGACTCATCCCAGACTATACTGATGATA
  TGCAGAGCCAGNATCCTTTCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002468
• Insert Size: 2550 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002468.2, NP_002459.1
• RefSeq Size: 2862 bp
• RefSeq ORF: 891 bp
• Locus ID: 4615
• UniProt ID: Q99836
• Cytogenetics: 3p22.2
• Domains: TIR, DEATH
• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis, Toll-like receptor signaling pathway
• Gene Summary: This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.