Nucleophosmin (NPM1) (NM_002520) Human Untagged Clone

CAT#: SC118573

NPM1 (untagged)-Human nucleophosmin (nucleolar phosphoprotein B23, numatrin) (NPM1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Nucleophosmin
• Synonyms: B23; NPM
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002520 edited
  GAATTCGGCACGAGGCCGTCCTGCGCGGTTGTTCTCTGGAGCAGCGTTCTTTTATCTCCG
  TCCGCCTTCTCTCCTACCTAAGTGCGTGCCGCCACCCGATGGAAGATTCGATGGACATGG
  ACATGAGCCCCCTGAGGCCCCAGAACTATCTTTTCGGTTGTGAACTAAAGGCCGACAAAG
  ATTATCACTTTAAGGTGGATAATGATGAAAATGAGCACCAGTTATCTTTAAGAACGGTCA
  GTTTAGGGGCTGGTGCAAAGGATGAGTTGCACATTGTTGAAGCAGAGGCAATGAATTACG
  AAGGCAGTCCAATTAAAGTAACACTGGCAACTTTGAAAATGTCTGTACAGCCAACGGTTT
  CCCTTGGGGGCTTTGAAATAACACCACCAGTGGTCTTAAGGTTGAAGTGTGGTTCAGGGC
  CAGTGCATATTAGTGGACAGCACTTAGTAGCTGTGGAGGAAGATGCAGAGTCAGAAGATG
  AAGAGGAGGAGGATGTGAAACTCTTAAGTATATCTGGAAAGCGGTCTGCCCCTGGAGGTG
  GTAGCAAGGTTCCACAGAAAAAAGTAAAACTTGCTGCTGATGAAGATGATGACGATGATG
  ATGAAGAGGATGATGATGAAGATGATGATGATGATGATTTTGATGATGAGGAAGCTGAAG
  AAAAAGCGCCAGTGAAGAAATCTATACGAGATACTCCAGCCAAAAATGCACAAAAGTCAA
  ATCAGAATGGAAAAGACTCAAAACCATCATCAACACCAAGATCAAAAGGACAAGAATCCT
  TCAAGAAACAGGAAAAAACTCCTAAAACACCAAAAGGACCTAGTTCTGTAGAAGACATTA
  AAGCAAAAATGCAAGCAAGTATAGAAAAAGGTGGTTCTCTTCCCAAAGTGGAAGCCAAAT
  TCATCAATTATGTGAAGAATTGCTTCCGGATGACTGACCAAGAGGCTATTCAAGATCTCT
  GGCAGTGGAGGAAGTCTCTTTAAGAAAATAGTTTAAACAATTTGTTAAAAAATTTTCCGT
  CTTATTTCATTTCTGTAACAGTTGATATCTGGCTGTCCTTTTTATAATGCAGAGTGAGAA
  CTTTCCCTACCGTGTTTGATAAATGTTGTCCAGGTTCTATTGCCAAGAATGTGTTGTCCA
  AAATGCCGTTTAGTTTTTAAAGATGGAACTCCACCCTTTGCTTGGTTTTAAGTATGTATG
  GAATGTTATGATAGGACATAGTAGTAGCGGTGGTCAGACATGGAAATGGTGGGGAGACAA
  AAATATACATGTGAAATAAAACTCAGTATTTTAATAAAGTAAAAAAAAAAAAAAAAAACT
  CGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002520 unedited
  GATATTTGTATACGACTTACTATAGGGNNCGGCCGCGNAATTCGGCACGAGGCCGTCCTG
  CGCGGTTGTTCTCTGGAGCAGCGTTCTTTTATCTCCGTCCGCCTTCTCTCCTACCTAAGT
  GCGTGCCGCCACCCGATGGAAGATTCGATGGACATGGACATGAGCCCCCTGAGGCCCCAG
  AACTATCTTTTCGGTTGTGAACTAAAGGCCGTACTTTGATTATCACTTTAAGGTGGATAA
  TGATGAAAATGAGCACCAGTTATCTTTAAGAACGGTCAGTTTAGGGGCTGGTGCAAAGGA
  TGAGTTGCACATTGTTGAAGCAGAGGCAATGAATTACGAAGGCAGTCCAATTAAAGTAAC
  ACTGGCAACTTTGAAAATGTCTGTACAGCCAACGGTTTCCCTTGGGGGCTTTGAAATAAC
  ACCACCAGTGGTCTTAAGGTTGAAGTGTGGTTCAGGGCCAGTGCATATTAGTGGACAGCA
  CTTAGTAGCTGTGGAGGAAGATGCAGAGTCAGAAGATGAAGAGGAGGAGGATGTGAAACT
  CTTAAGTATATCTGGAAAGCGGTCTGCCCCTGGAGGTGGTAGCAAGGTTCCACAGAAAAA
  AGTAAAACTTGCTGCTGATGAAGATGATGACGATGATGATGAAGAGGATGATGATGAAGA
  TGATGATGATGATGATTCTGATGATGAGGAAGCTGAAGACAAAGCGCCAGTGAAGAAATC
  TATACGAGATACTCCAGCCAAAAATGCACAAAAGTCAAATCAGAATGGGAAAGACTCANA
  ACCATCATCACCACCCAGATCAAAAGGACAAGAATCCTTCAAGAAACAGGAATAAACTTC
  TAAACACCCAAAGCACCTAGTTCTGTAGAAGACATTTAAAGCAAAATGCAAGCAGTATAG
  AAAAAGGTGGGTCTCCTCCCAAAGGGGGAGCCCAATTCATCATATGTGAAGAATGCTCCC
  GTAGACTGACCANAGCTTN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002520 unedited
  TTTCCCCCCCTCCNCCCCCCCCCCCCNCGTTGCGTTTGCTTTGGACCGCGGCCGCATCCT
  ACGATCGAGTTTTTTTTTTTTTTTTTTACTTTATTAAAAACTGAGTTTTATTCTCACATG
  TATATTTTTGTACTCCCCACCATTTCCATGTCTGACCACCGCTACTACTATGTCCTATCA
  TAACATTCCATACATACTTAAAACCAAGCAAAGGGTGGAGTTCCATCTTTAAAAACTAAA
  CGGCATTTTGGACAACACATTCTTGGCAATAGAACCTGGACAACATTTATCAAACACGGT
  AGGGAAAGTTCTCACTCTGCATTATAAAAAGGACAGTCAGATATCAACTGTTACAGAAAT
  GAAATAAGACGGAAAATTTTTTAACAAATTGTTTAAACTATTTTCTTAAAGAGACTTCCT
  CCACTGCCAGAGATCTTGAATAGCCTCTTGGTCAGTCATCCGGAAGCAATTCTTCACATA
  ATTGATGAATTTGGCTTCCACTTTGGGAAGAGAACCACCTTTTTCTATACTTGCTTGCAT
  TTTTGCTTTAATGTCTTCTACAGAACTAGGTCCTTTTGGTGTTTTAGGAGTTTTTTCCTG
  TTTCTTGAAGGATTCTTGTCCTTTTGATCTTGGCGTTGACGATGGTTTTGAGTCTTTTCC
  ATTCTGATTTGACTTTTGTGCATTTTTGGCTGGAGTATCCTCGATCGATTTCTTCACTGG
  CGCTTTTTCTTCAGCTTTCTCATCATCAAAAANATCATCATCATCACTCTCCACATCATT
  CCTCTTCACCACCATCGGCATCATTCTTCATCAACAGCAAAGTTTTACTTCTTTTTTGGG
  GAACCTTTGCTACAACCTTCAGGGGGCAGAACCGCTTTTTCCAAAAATTATTTAAGGAGT
  TTCCCATCCCTCCCTCCCTCTTTCATCTTCTGAACACTGGCAATCTTCCTCCACAAGATA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002520
• Insert Size: 1310 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002520.4, NP_002511.1
• RefSeq Size: 1350 bp
• RefSeq ORF: 885 bp
• Locus ID: 4869
• UniProt ID: P06748
• Cytogenetics: 5q35.1
• Domains: Nucleoplasmin
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 7 both encode the same isoform (1).