FURIN (NM_002569) Human Untagged Clone

SKU SC118550

FURIN (untagged)-Human furin (paired basic amino acid cleaving enzyme) (FURIN)

Product Data

• Type: Human Untagged Clone
• Target Symbol: FURIN
• Synonyms: FUR; PACE; PCSK3; SPC1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118550 sequence for NM_002569 edited (data generated by NextGen Sequencing); ATGGAGCTGAGGCCCTGGTTGCTATGGGTGGTAGCAGCAACAGGAACCTTGGTCCTGCTA; GCAGCTGATGCTCAGGGCCAGAAGGTCTTCACCAACACGTGGGCTGTGCGCATCCCTGGA; GGCCCAGCGGTGGCCAACAGTGTGGCACGGAAGCATGGGTTCCTCAACCTGGGCCAGATC; TTCGGGGACTATTACCACTTCTGGCATCGAGGAGTGACGAAGCGGTCCCTGTCGCCTCAC; CGCCCGCGGCACAGCCGGCTGCAGAGGGAGCCTCAATGGCTGGAACAGCAGGTGGCAAAG; CGACGGACTAAACGGGACGTGTACCAGGAGCCCACAGACCCCAAGTTTCCTCAGCAGTGG; TACCTGTCTGGTGTCACTCAGCGGGACCTGAATGTGAAGGCGGCCTGGGCGCAGGGCTAC; ACAGGGCACGGCATTGTGGTCTCCATTCTGGACGATGGCATCGAGAAGAACCACCCGGAC; TTGGCAGGCAATTATGATCCTGGGGCCAGTTTTGATGTCAATGACCAGGACCCTGACCCC; CAGCCTCGGTACACACAGATGAATGACAACAGGCACGGCACACGGTGTGCGGGGGAAGTG; GCTGCGGTGGCCAACAACGGTGTCTGTGGTGTAGGTGTGGCCTACAACGCCCGCATTGGA; GGGGTGCGCATGCTGGATGGCGAGGTGACAGATGCAGTGGAGGCACGCTCGCTGGGCCTG; AACCCCAACCACATCCACATCTACAGTGCCAGCTGGGGCCCCGAGGATGACGGCAAGACA; GTGGATGGGCCAGCCCGCCTCGCCGAGGAGGCCTTCTTCCGTGGGGTTAGCCAGGGCCGA; GGGGGGCTGGGCTCCATCTTTGTCTGGGCCTCGGGGAACGGGGGCCGGGAACATGACAGC; TGCAACTGCGACGGCTACACCAACAGTATCTACACGCTGTCCATCAGCAGCGCCACGCAG; TTTGGCAACGTGCCGTGGTACAGCGAGGCCTGCTCGTCCACACTGGCCACGACCTACAGC; AGTGGCAACCAGAATGAGAAGCAGATCGTGACGACTGACTTGCGGCAGAAGTGCACGGAG; TCTCACACGGGCACCTCAGCCTCTGCCCCCTTAGCAGCCGGCATCATTGCTCTCACCCTG; GAGGCCAATAAGAACCTCACATGGCGGGACATGCAACACCTGGTGGTACAGACCTCGAAG; CCAGCCCACCTCAATGCCAACGACTGGGCCACCAATGGTGTGGGCCGGAAAGTGAGCCAC; TCATATGGCTACGGGCTTTTGGACGCAGGCGCCATGGTGGCCCTGGCCCAGAATTGGACC; ACAGTGGCCCCCCAGCGGAAGTGCATCATCGACATCCTCACCGAGCCCAAAGACATCGGG; AAACGGCTCGAGGTGCGGAAGACCGTGACCGCGTGCCTGGGCGAGCCCAACCACATCACT; CGGCTGGAGCACGCTCAGGCGCGGCTCACCCTGTCCTATAATCGCCGTGGCGACCTGGCC; ATCCACCTGGTCAGCCCCATGGGCACCCGCTCCACCCTGCTGGCAGCCAGGCCACATGAC; TACTCCGCAGATGGGTTTAATGACTGGGCCTTCATGACAACTCATTCCTGGGATGAGGAT; CCCTCTGGCGAGTGGGTCCTAGAGATTGAAAACACCAGCGAAGCCAACAACTATGGGACG; CTGACCAAGTTCACCCTCGTACTCTATGGCACCGCCCCTGAGGGGCTGCCCGTACCTCCA; GAAAGCAGTGGCTGCAAGACCCTCACGTCCAGTCAGGCCTGTGTGGTGTGCGAGGAAGGC; TTCTCCCTGCACCAGAAGAGCTGTGTCCAGCACTGCCCTCCAGGCTTCGCCCCCCAAGTC; CTCGATACGCACTATAGCACCGAGAATGACGTGGAGACCATCCGGGCCAGCGTCTGCGCC; CCCTGCCACGCCTCATGTGCCACATGCCAGGGGCCGGCCCTGACAGACTGCCTCAGCTGC; CCCAGCCACGCCTCCTTGGACCCTGTGGAGCAGACTTGCTCCCGGCAAAGCCAGAGCAGC; CGAGAGTCCCCGCCACAGCAGCAGCCACCTCGGCTGCCCCCGGAGGTGGAGGCGGGGCAA; CGGCTGCGGGCAGGGCTGCTGCCCTCACACCTGCCTGAGGTGGTGGCCGGCCTCAGCTGC; GCCTTCATCGTGCTGGTCTTCGTCACTGTCTTCCTGGTCCTGCAGCTGCGCTCTGGCTTT; AGTTTTCGGGGGGTGAAGGTGTACACCATGGACCGTGGCCTCATCTCCTACAAGGGGCTG; CCCCCTGAAGCCTGGCAGGAGGAGTGCCCGTCTGACTCAGAAGAGGACGAGGGCCGGGGC; GAGAGGACCGCCTTTATCAAAGACCAGAGCGCCCTCTGA; ; Clone variation with respect to NM_002569.2; 277 g=>-;278 t=>-;279 a=>-;280 c=>-;281 a=>-;282 g=>-;1851 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002569 unedited; CCCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGA; GCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAAT; TCGGCACGAGGCCAAGGAGACGGGCGCTCCAGGGTCCCAGCCACCTGTCCCCCCCATGGA; GCTGAGGCCCTGGTTGCTATGGGTGGTAGCAGCAACAGGAACCTTGGTCCTGCTAGCAGC; TGATGCTCAGGGCCAGAAGGTCTTCACCAACACGTGGGCTGTGCGCATCCCTGGAGGCCC; AGCGGTGGCCAACAGTGTGGCACGGAAGCATGGGTTCCTCAACCTGGGCCAGATCTTCGG; GGACTATTACCACTTCTGGCATCGAGGAGTGACGAAGCGGTCCCTGTCGCCTCACCGCCC; GCGGCACAGCCGGCTGCAGAGGGAGCCTCAATGGCTGGAACAGCAGGTGGCAAAGCGACG; GACTAAACGGGACGTGTACCAGGAGCCCACAGACCCCAAGTTTCCTCAGCAGTGGTACCT; GTCTGGTGTCACTCAGCGGGACCTGAATGTGAAGGCGGCCTGGGCGCAGGGCTACACAGG; GCACGGCATTGTGGTCTCCATTCTGGACGATGGCATCGAGAAGAACCACCCGGACTTGGC; AGGCAATTATGATCCTGGGGCCAGTTTTGATGTCAATGACCAGGACCCTGACCCCCAGCC; TCGGTACACACAGATGAATGACAACAGGCACGGCACACGGTGTGCGGNGGAAGTGGCTGC; GGTGGCCAACAAACGTGTCTGTGGTGTANGGTGTGGCCCTACACGCCCGCATTGGAGGGG; TGCGCATGCTGGATGGCGAGGTGACAGATGCAGTGGAGGCACGCTCGCTGGGCCTGAACC; CCAACCCATNCCATCTACGTGCCAGCTGGNGCCCGAGGATGACGCAGACATNGATGGGCC; ACCCGCTCGCGAGGAGCCTCTCCGTGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002569 unedited; GGTTTGTTCAGCCCGATCATGGGTTTAAAAAAAACCCATTATCTCATGTCCATTAAATTT; AACCAGCAATGCTGGGCCTGTTTAAATTACAAGAAAAAAATCACTGTGCACCAACCCAGT; ATCTTACAAAACCAGCCGGGCTGGCCACGAGGGCAGGGGGATGGGAGAGGAGGGGGCACC; CCTGGGCAGATGGCTGGGTACCTGGAATGGCTTGGGTAGAAAAGAAAAGGGGACTCCCAG; ATGGGAGGGGACTGATTGTCCTAATGGGAGGGGTGCAAAAGGCACGTCAGATGAGGAGGT; TTGAGGGGGGCATGACAGGTCAGTGCTTTCTCCACCAGGGCACAGTGTTGGGGGGGCTCA; GTGCCACCACCTGCCCACCTCTGGCACTTGGAGAGCCAGTGACCTATGAACCCCCGGCAT; GGCGAGCCCCACTTGAAACACAGATGTCAGCCTGCCCCTCCCACTGGGACCCCCTTTCCT; TTGAGCCCGCCTGGCTGGCCCGCCCTCTTCAGGACTTGGGGGCTCAACTTATTCCTCAAT; TCTTAAGCAACATTGCTGCAAGCCCCCACACCTTAAGGCCAGGGCCAACCAGGGAGGAGC; CAGGCGGGACGGTGGTGGGTGAAAACGTGGGTTGGTCCAGCTGGCTCCCCCCATTTAGAA; TACTAAAACCGAAATCATCCGGCACTCTACGGGGGAAGTATCCCCAGGGTGGAAAAATTT; ATCGCTTAAAACGGGCCCCTTGGGTATCCTCAACCAGGGGGAAAAACCACATATTTGTGG; TGGGGAAGGTCACAAAGAAGGCCGGGCGTGGGGCCATACGCGCGTCTTCCCAACACACCC; CACCCTGGGGGCTTCGGAGTTGTGTGGAGGTCCCAATAAGCCTAGGCGAGCCCGGGCGCC; TAAAAGAAACAGGGTCCATGTGGGCCCCTGTACCGAAGAGAAGGCGGGGTCTCAGAAGAA; GGCGACGCGGTGGTTCAAGCAGACACCACCNACATAAGTGTTCACCCACCACAGTGTATG; CTGTTAGTGGTTGTCGCCACACACGGGGTGGCCCCCACGGGCGATGGGGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002569
• Insert Size: 2385 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002569.2, NP_002560.1
• RefSeq Size: 4180 bp
• RefSeq ORF: 2385 bp
• Locus ID: 5045
• UniProt ID: P09958
• Cytogenetics: 15q26.1
• Domains: FU, Peptidase_S8, P_proprotein
• Protein Families: Druggable Genome, Protease, Transmembrane
• Summary: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. Like other members of this convertase family, the product of this gene specifically cleaves substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140, and may play a role in tumor progression. Unlike SARS-CoV and other coronaviruses, the spike protein of SARS-CoV-2 is thought to be uniquely cleaved by this protease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2020]; Transcript Variant: This variant (1) represents the predominant transcript. Variants 1, 2, and 3 encode the same protein.