PBP (PEBP1) (NM_002567) Human Untagged Clone

CAT#: SC118548

PEBP1 (untagged)-Human phosphatidylethanolamine binding protein 1 (PEBP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PBP
• Synonyms: HCNP; HCNPpp; HEL-210; HEL-S-34; HEL-S-96; PBP; PEBP; PEBP-1; RKIP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118548 sequence for NM_002567 edited (data generated by NextGen Sequencing)
  ATGCCGGTGGACCTCAGCAAGTGGTCCGGGCCCTTGAGCCTGCAAGAAGTGGACGAGCAG
  CCGCAGCACCCGCTGCATGTCACCTACGCCGGGGCGGCGGTGGACGAGCTGGGCAAAGTG
  CTGACGCCCACCCAGGTTAAGAATAGACCCACCAGCATTTCGTGGGATGGTCTTGATTCA
  GGGAAGCTCTACACCTTGGTCCTGACAGACCCGGATGCTCCCAGCAGGAAGGATCCCAAA
  TACAGAGAATGGCATCATTTCCTGGTGGTCAACATGAAGGGCAATGACATCAGCAGTGGC
  ACAGTCCTCTCCGATTATGTGGGCTCGGGGCCTCCCAAGGGCACAGGCCTCCACCGCTAT
  GTCTGGCTGGTTTACGAGCAGGACAGGCCGCTAAAGTGTGACGAGCCCATCCTCAGCAAC
  CGATCTGGAGACCACCGTGGCAAATTCAAGGTGGCGTCCTTCCGTAAAAAGTATGAGCTC
  AGGGCCCCGGTGGCTGGCACGTGTTACCAGGCCGAGTGGGATGACTATGTGCCCAAACTG
  TACGAGCAGCTGTCTGGGAAGTAG
  
  Clone variation with respect to NM_002567.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002567 unedited
  ACTGCGATTTGTAACCGACTTACTGATAGGGCGGCCGCGCAATCGGCACGAGGCGCCCGC
  GCCTGGCCTACCGCGGCACTCCCGGCTGCACGCTCTGCTTGGCCTCGCCATGCCGGTGGA
  CCTCAGCAAGTGGTCCGGGCCCTTGAGCCTGCAAGAAGTGGACGAGCAGCCGCAGCACCC
  GCTGCATGTCACCTACGCCGGGGCGGCGGTGGTACTAGCTGGGCAAAGTGCTGACGCCCA
  CCCAGGTTAAGAATAGACCCACCAGCATTTCGTGGGATGGTCTTGATTCAGGGAAGCTCT
  ACACCTTGGTCCTGACAGACCCGGATGCTCCCAGCAGGAAGGATCCCAAATACAGAGAAT
  GGCATCATTTCCTGGTGGTCAACATGAAGGGCAATGACATCAGCAGTGGCACAGTCCTCT
  CCGATTATGTGGGCTCGGGGCCTCCCAAGGGCACAGGCCTCCACCGCTATGTCTGGCTGG
  TTTACGAGCAGGACAGGCCGCTAAAGTGTGACGAGCCCATCCTCAGCAACCGATCTGGAG
  ACCACCGTGGCAAATTCAAGGTGGCGTCCTTCCGTAAAAAGTATGAGCTCAGGGCCCCGG
  TGGCTGGCACGTGTTACCAGGCCGAGTGGGATGACTATGTGCCCAAACTGTACGAGCAGC
  TGTCTGGGAAGTAGGGGGTTAGCTTGCGGACCTGAACTGTCCTGGAGGCCCCAAGCCATG
  TTCCCCAGTTCAGTGTTGCATGTATAATAGATTTCTCCTCTTCCTGCCCNACTTGGCATG
  GGTGAGACCTGACCAGTCAGATGGTAGNTGAGGGTGACTTTTCCTGCTGCCTGGCCTTTA
  TATTTTACTCACTCCTCTGATTATGTTTTGATCAAATTTGACTTCATTTGGGGGGTATTT
  TGGTACTGTGATGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002567 unedited
  GGCAACCTCCAGGTCCAGAAGNAGCACTGGGGCGGGGTCACAGGGATGCCACCCGGGATC
  TGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT
  TTAAACCTCCCTTAAAGATTCTTTGATGCTTTGCTCTATCACTGTAGACCTGGTCTTTTT
  CCCCCCAGTTTTTTCTTTTTTACATTCTGGGTTGCTATTTTCAGATTAATAATTTGATGA
  CCCCATCACAGTACCAAAATACCCCCCAAAATGAAGTTCAAATTTGATCAAAACATAAAT
  CAGAGTGAGTGAGTAAAATTATAAAGGCCAGGCAGCAGGAAAAGTCACCCTCAACTACCA
  TCTGACTGGTCAGGTCTCACCCATGCCAAGGGGGGCAGGAAGAGGAGAAATCTATTATAC
  ATGCAACACTGAACTGGGGAACATGGCTTGGGGCCTCCAGGACAGTTCAGGTCCCCAAGC
  TAACCCCCTACTTCCCAGACAGCTGCTCGTACAGTTTGGGCACATAGTCATCCCACTCGG
  CCTGGTAACACGTGCCAGCCACCGGGGCCCTGAGCTCATACTTTTTACGGAAGGACGCCA
  CCTTGAATTTGCCACGNGTGGTCTCCAGAATCGGTTGCTGAAGGATGGGCTCGTCACACT
  TTANCGGGCCTGTCCTGCTNCGTAACAGCCAACATTACGGGGGGAAGGCCTGGGCCCTTG
  GGAGGCCCCGACCCACATATCGGNAGAGGACCGGGCCACTGGTGATGTCATTGCCCTTCA
  TGTTGACCACAGAAATGATGCCATTCTCTGTATTTGGGATCCTTCCTGCTGGGACATACC
  GGGTCTGTAGGACCAAGGTGTAAAACTTCCTGATCAAGACATCCACGATGGCGGGTGGGC
  TATCTAACCGGGGGNGGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002567
• Insert Size: 1040 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002567.2, NP_002558.1
• RefSeq Size: 1507 bp
• RefSeq ORF: 564 bp
• Locus ID: 5037
• UniProt ID: P30086
• Cytogenetics: 12q24.23
• Domains: PBP
• Gene Summary: This gene encodes a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. The encoded protein can be further processed to form a smaller cleavage product, hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in neural development. This gene has been implicated in numerous human cancers and may act as a metastasis suppressor gene. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Jul 2015]