PFKP (NM_002627) Human Untagged Clone

CAT#: SC118507

PFKP (untagged)-Human phosphofructokinase, platelet (PFKP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PFKP
• Synonyms: ATP-PFK; PFK-C; PFK-P; PFKF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118507 sequence for NM_002627 edited (data generated by NextGen Sequencing)
  ATGGACGCGGACGACTCCCGGGCCCCCAAGGGCTCCTTGCGGAAGTTCCTGGAGCACCTC
  TCCGGGGCCGGCAAGGCCATCGGCGTGCTGACCAGCGGCGGGGATGCTCAAGGTATGAAC
  GCTGCCGTCCGTGCCGTGGTGCGCATGGGTATCTACGTGGGGGCCAAGGTGTACTTCATC
  TACGAGGGCTACCAGGGCATGGTGGACGGAGGCTCAAACATCGCAGAGGCCGACTGGGAG
  AGTGTCTCCAGCATCCTGCAAGTGGGCGGGACGATCATTGGCAGTGCGCGGTGCCAGGCC
  TTCCGCACGCGGGAAGGCCGCCTGAAGGCTGCTTGCAACCTGCTGCAGCGCGGCATCACC
  AACCTGTGTGTGATCGGCGGGGACGGGAGCCTCACCGGGGCCAACCTCTTCCGGAAGGAG
  TGGAGTGGGCTGCTGGAGGAGCTGGCCAGGAACGGCCAGATCGATAAGGAGGCCGTGCAG
  AAGTACGCCTACCTCAACGTGGTGGGCATGGTGGGCTCCATCGACAATGATTTCTGCGGC
  ACCGACATGACCATCGGCACGGACTCCGCCCTGCACAGGATCATCGAGGTCGTCGACGCC
  ATCATGACCACGGCCCAGAGCCACCAGAGGACCTTCGTTCTGGAGGTGATGGGACGACAC
  TGTGGGTACCTGGCCCTGGTGAGTGCCTTGGCCTGCGGTGCGGACTGGGTGTTCCTTCCA
  GAATCTCCACCAGAGGAAGGCTGGGAGGAGCAGATGTGTGTCAAACTCTCGGAGAACCGT
  GCCCGGAAAAAAAGGCTGAATATTATTATTGTGGCTGAAGGAGCAATTGATACCCAAAAT
  AAACCCATCACCTCTGAGAAAATCAAAGAGCTTGTCGTCACGCAGCTGGGCTATGACACA
  CGTGTGACCATCCTCGGGCACGTGCAGAGAGGAGGGACCCCTTCGGCATTTGACAGGATC
  TTGGCCAGCCGCATGGGAGTGGAGGCAGTCATCGCCTTGCTAGAGGCCACCCCGGACACC
  CCAGCTTGCGTCGTGTCACTGAACGGGAACCACGCCGTGCGCCTGCCGCTGATGGAGTGC
  GTGCAGATGACTCAGGATGTGCAGAAGGCGATGGACGAGAGGAGATTTCAAGATGCGGTT
  CGACTCCGAGGGAGGAGCTTTGCGGGCAACCTGAACACCTACAAGCGACTTGCCATCAAG
  CTGCCGGATGATCAGATCCCAAAGACCAATTGCAACGTAGCTGTCATCAACGTGGGGGCA
  CCCGCGGCTGGGATGAACGCGGCCGTACGCTCAGCTGTGCGCGTGGGCATTGCCGACGGC
  CACAGGATGCTCGCCATCTATGATGGCTTTGACGGCTTCGCCAAGGGCCAGATCAAAGAA
  ATCGGCTGGACAGATGTCGGGGGCTGGACCGGCCAAGGAGGCTCCATTCTTGGGACAAAA
  CGCGTTCTCCCGGGGAAGTACTTGGAAGAGATCGCCACACAGATGCGCACGCACAGCATC
  AACGCGCTGCTGATCATCGGTGGATTCGAGGCCTACCTGGGACTCCTGGAGCTGTCAGCC
  GCCCGGGAGAAGCACGAGGAGTTCTGTGTCCCCATGGTCATGGTTCCCGCTACTGTGTCC
  AACAATGTGCCGGGTTCCGATTTCAGCATCGGGGCAGACACCGCCCTGAACACTATCACC
  GACACCTGCGACCGCATCAAGCAGTCCGCCAGCGGAACCAAGCGGCGCGTGTTCATCATC
  GAGACCATGGGCGGCTACTGTGGCTACCTGGCCAACATGGGGGGGCTCGCGGCTGGAGCT
  GATGCCGCATACATTTTCGAAGAGCCCTTCGACATCAGGGATCTGCAGTCCAACGTGGAG
  CACCTGACGGAGAAAATGAAGACCACCATCCAGAGAGGCCTTGTGCTCAGAAATGAGAGC
  TGCAGTGAAAACTACACCACCGACTTCATTTACCAGCTGTATTCAGAAGAGGGCAAAGGC
  GTGTTTGACTGCAGGAAGAACGTGCTGGGTCACATGCAGCAGGGTGGGGCACCCTCTCCA
  TTTGATAGAAACTTTGGAACCAAAATCTCTGCCAGAGCTATGGAGTGGATCACTGCAAAA
  CTCAAGGAGGCCCGGGGCAGAGGAAAAAAATTTACCACCGATGATTCCATTTGTGTGCTG
  GGAATAAGCAAAAGAAACGTTATTTTTCAACCTGTGGCAGAGCTGAAGAAGCAAACGGAT
  TTTGAGCACAGGATTCCCAAAGAACAGTGGTGGCTCAAGCTACGGCCCCTCATGAAAATC
  CTGGCCAAGTACAAGGCCAGCTATGACGTGTCGGACTCAGGCCAGCTGGAACATGTGCAG
  CCCTGGAGTGTCTGA
  
  Clone variation with respect to NM_002627.4
  951 c=>t;1281 a=>g;1794 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002627 unedited
  GTCAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCCCATTGCC
  TGCTGCGCACCCGGACGTGCGGCTCCCCTCGGCCTCCTCGCCATGGACGCGGACGACTCC
  CGGGCCCCCAGGGCTCCTTGCGGAAGTTCCTGGAGCACCTCTCCGGGGCCGGCAAGGCCA
  TCGGCGTGCTGACCAGCGGCGGGGATGCTCAAGGTATGAACGCTGCCGTCCGTGCCGTGG
  TGCGCATGGGTATCTACGTGGGGGCCAAGGTGTACTTCATCTACGAGGGCTACCAGGGCA
  TGGTGGACGGAGGCTCAAACATCGCAGAGGCCGACTGGGAGAGTGTCTCCAGCATCCTGC
  AAGTGGGCGGGACGATCATTGGCAGTGCGCGGTGCCAGGCCTTCCGCACGCGGGAAGGCC
  GCCTGAAGGCTGCTTGCAACCTGCTGCAGCGCGGCATCACCAACCTGTGTGTGATCGGCG
  GGGACGGGAGCCTCACCGGGGCCAACCTCTTCCGGAAGGAGTGGAGTGGGCTGCTGGAGG
  AGCTGGCCAGGAACGGCCAGATCGATAAGGAGGCCGTGCAGAAGTACGCCTACCTCAACG
  TGGTGGGCATGGTGGGCTCCATCGACAATGATTTCTGCGGCACCGACATGACCATCGGCA
  CGGACTCCGCCCTGCACAGGATCATCGAGGTCGTCGACGCCATCATGACCACGGCCCAGA
  GCCACCAGAGGACCTTCGTTCTGGNAGGTGATGGGACGACACTGTGGGTACCTGGCCCTG
  GTGAGTGCCTTGGCCTGCGGTGCGACTCTGGTTGTTCCTTNNCAGAATCTCCACCANAGA
  ANGNCTGGGAGGAGCANNATGTGTGTCAAACTCTCGGAGAACCGTGCCCGGAAAAAAGGC
  TGATTTTATATTGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002627 unedited
  CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGAGTTGTAGGCAAATGTTTA
  ATTCTGCTCATATGCACATCTGAAAGCATGAGACACACTCCACAGACAGCACGCACTGGG
  GCTGGTGGGGCAGATGGGCACTCGCCGATTAGGTATTAATGTTAATAATACGTGCATAAA
  GTGCTGATAAAATAACTTAAGTGTTACAAAAACAGACAGTCCACGGTGGCTGCAGGCACA
  TGCAGGCGGGACTGGGTCAGACACTCCAGGGCTGCACATGTTCCAGCTGGCCTGAGTCCG
  ACACGTCATAGCTGGCCTTGTACTTGGCCAGGATTTTCATGAGGGGCCGTAGCTTGAGCC
  ACCACTGTTCTTTGGGAATCCTGTGCTCAAAATCCGTTTGCTTCTTCAGCTCTGCCACAG
  GTTGAAAAATAACGTTTCTTTTGCTTATTCCCAGCACACAAATGGAATCATCGGTGGTAA
  ATTTTTTTCCTCTGCCCCGGGCCTCCTTGAGTTTTGCAGTGATCCACTCCATAGCTCTGG
  CAGAGATTTTGGTTCCAAAGTTTCTATCAAATGGAGAGGGTGCCCCACCCTGCTGCATGT
  GACCCAGCACGTTCTTCCTGCAGTCAAACACGCCTTTGCCCTCTTCTGAATACAGCTGGT
  AAATGAAGTCGGTGGTGTAGTTTTCACTGCAGCTCTCATTTCTGAGCACAAGGCCTCTCT
  GGATGGTGGTCTTCATTCTCTCCGTCACGTGCTCCACGTTTGACTGCACATCCCTGATGT
  CNAAAGGCTCTTCCAAATGTATGCGGCATCAGCTCCAGCCGCGAGCCCCCCCATTTTGGC
  CAAGTACCCCAGTTACCGCCCATGTTCTTNATGATGACACCCCCGCTTGGTTCCCTTGCC
  GACTGTTTGATCCGTCCAGGTGTCGGAAATTGTTAGGCCGTGTTTGCCCGATGTGAAATC
  GAACCGGCAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002627
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002627.3, NP_002618.1
• RefSeq Size: 2628 bp
• RefSeq ORF: 2355 bp
• Locus ID: 5214
• UniProt ID: Q01813
• Cytogenetics: 10p15.2
• Domains: PFK
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway
• Gene Summary: This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (1) encodes the longest isoform (1).