Perilipin-1 (PLIN1) (NM_002666) Human Untagged Clone

CAT#: SC118500

PLIN1 (untagged)-Human perilipin 1 (PLIN1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Perilipin-1
• Synonyms: FPLD4; PERI; PLIN
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118500 sequence for NM_002666 edited (data generated by NextGen Sequencing)
  ATGGCAGTCAACAAAGGCCTCACCTTGCTGGATGGAGACCTCCCTGAGCAGGAGAATGTG
  CTGCAGCGGGTCCTGCAGCTGCCGGTGGTGAGTGGCACCTGCGAATGCTTCCAGAAGACC
  TACACCAGCACTAAGGAAGCCCACCCCCTGGTGGCCTCTGTGTGCAATGCCTATGAGAAG
  GGCGTGCAGAGCGCCAGTAGCTTGGCTGCCTGGAGCATGGAGCCGGTGGTCCGCAGGCTG
  TCCACCCAGTTCACAGCTGCCAATGAGCTGGCCTGCCGAGGCTTGGACCACCTGGAGGAA
  AAGATCCCCGCCCTCCAGTACCCCCCTGAAAAGATTGCTTCTGAGCTGAAGGACACCATC
  TCCACCCGCCTCCGCAGTGCCAGAAACAGCATCAGCGTTCCCATCGCGAGCACTTCAGAC
  AAGGTCCTGGGGGCCGCTTTGGCCGGGTGCGAGCTTGCCTGGGGGGTGGCCAGAGACACT
  GCGGAATTTGCTGCCAACACTCGAGCTGGCCGACTGGCTTCTGGAGGGGCCGACTTGGCC
  TTGGGCAGCATTGAGAAGGTGGTGGAGTACCTCCTCCCTGCAGACAAGGAAGAGTCAGCC
  CCTGCTCCTGGACACCAGCAAGCCCAGAAGTCTCCCAAGGCCAAGCCAAGCCTCTTGAGC
  AGGGTTGGGGCTCTGACCAACACCCTCTCTCGATACACCGTGCAGACCATGGCCCGGGCC
  CTGGAGCAGGGCCACACCGTGGCCATGTGGATCCCAGGCGTGGTGCCCCTGAGCAGCCTG
  GCCCAGTGGGGTGCCTCAGTGGCCATGCAGGCGGTGTCCCGGCGGAGGAGCGAAGTGCGG
  GTACCCTGGCTGCACAGCCTCGCAGCCGCCCAGGAGGAGGATCATGAGGACCAGACAGAC
  ACGGAGGGAGAGGACACGGAGGAGGAGGAAGAATTGGAGACTGAGGAGAACAAGTTCAGT
  GAGGTAGCAGCCCTGCCAGGCCCTCGAGGCCTCCTGGGTGGTGTGGCACATACCCTGCAG
  AAGACCCTCCAGACCACCATCTCGGCTGTGACATGGGCACCTGCAGCTGTGCTGGGCATG
  GCAGGGAGGGTGCTGCACCTCACACCAGCCCCTGCTGTCTCCTCAACCAAGGGGAGGGCC
  ATGTCCCTATCAGATGCCCTGAAGGGCGTTACTGACAACGTGGTGGACACAGTGGTGCAT
  TACGTGCCGCTCCCCAGGCTGTCGCTGATGGAGCCCGAGAGCGAATTCCGGGACATCGAC
  AACCCACCAGCCGAGGTCGAGCGCCGGGAGGCGGAGCGCAGAGCGTCTGGGGCGCCGTCC
  GCCGGCCCGGAGCCCGCCCCGCGTCTCGCACAGCCCCGCCGCAGCCTGCGCAGCGCGCAG
  AGCCCCGGCGCGCCCCCCGGCCCGGGNNNNNNGGACGAAGTCGCCACGCCCGCAGCGCCG
  CGCCCGGGCTTCCCGGCCGTGCCCCGCGAGAAGCCAAAGCGCAGGGTCAGCGACAGCTTC
  TTCCGGCCCAGCGTCATGGAGCCCATCCTGGGCCGCACGCATTACAGCCAGCTGCGCAAG
  AAGAGCTGA
  
  Clone variation with respect to NM_002666.4
  580 c=>g;1407 c=>n;1408 c=>n;1409 t=>n;1410 g=>n;1411 g=>n;1412 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002666 unedited
  GGGGGTCAGGATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGACAGGA
  ATTGTTTCTGCCTGAGGAGACACTCTGCAGCCTGGGCTCTGTGAGACTGAGGTGGCGGTC
  AGCCGGAGTGAGTGTTGGGGTCCTGGGGCACCTGCCTTACATGGCTTGTTTATGAACATT
  AAAGGGAAGAAGTTGAAGCTTGAGGAGCGAGGATGGCAGTCAACAAAGGCCTCACCTTGC
  TGGATGGAGACCTCCCTGAGCAGGAGAATGTGCTGCAGCGGGTCCTGCAGCTGCCGGTGG
  TGAGTGGCACCTGCGAATGCTTCCAGAAGACCTACACCAGCACTAAGGAAGCCCACCCCC
  TGGTGGCCTCTGTGTGCAATGCCTATGAGAAGGGCGTGCAGAGCGCCAGTAGCTTGGCTG
  CCTGGAGCATGGAGCCGGTGGTCCGCAGGCTGTCCACCCAGTTCACAGCTGCCAATGAGC
  TGGCCTGCCGAGGCTTGGACCACCTGGAGGAAAAGATCCCCGCCCTCCAGTACCCCCCTG
  AAAAGATTGCTTCTGAGCTGAAGGACACCATCTCCACCCGCCTCCGCAGTGCCAGAAACA
  GCATCAGCGTTCCCATCGCGAGCACTTCAGACAAGGTCCTGNGGGCCGCTTTGGCCGGGT
  GCGAGCTTGCCTGNGGGGGTGGCCAGAGACACTGCGGAATTTGCTGCCAACACTCGAGCT
  GGCCGACTGGCTTCTGGAGGGGCCGACTTGGCCTTGGGCAGCATTGAGAAGGTGGNTGGA
  GTACCTNCTCCCTGCAGACAGGAAGAGTCAGNCCCTGCTCCTGGACACCAGCAGCCCAGA
  AGTCTCCCAGGCCAGCCCCAGCTCTGAGCAGGTNTGGGGCTCTGACCACACCTCTCTCGA
  ACAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002666 unedited
  NCTTACTTGNNACCGCGGCCCGCATCTANATCGAGTTTTTTTTTTTTTTTTTTTTGATAA
  GGACATTTATTATAGCATCGTTTGCAGTAGCAAAAAAGAAAAAAAAGGGAAACAACTTAA
  AAGTCCATCATTAGGGAACTAGTTAAATCAAATAAGGCGTATCCATATTATGCAGTAGTT
  ATTATGTGGCCATCAAAAAGAGCGAGTGGGATTTATAATGTGATACAAAAATCTCTCCAA
  GCTGTATTATTAAGTGAAAAAAAGCAGGCATGCGTAACACCCCACAGCTTGTGTAAACAC
  AAGCGGGGAGTGCATACACACGTGCCTGCAGGTGCATAGCCCTGCATACACAAGAGATGG
  CACCGTGGTGGTTTTCAATGAAGGGGAACAGGGGAGCTCGGGGAGAAAGACACACATCCT
  TTTGCAATATTTGAATTCTGTAATTGTATGAATGCATTTTCTAGATTTATCAAATATTAA
  CATTTCGAAGACTAGGGTTGGGGATGAACTGTGGCTATACATAAAGTCTATATATCATCA
  CCATTTTGGTTCCCCAGCATCAAAAGAGTGACTATGCAGGTGAAGGCAGTAATGATGCAA
  ATGGAAATGTGGCTGTGAGCAAGCAGCTGGCTCTACAAAGCACACAGGCCTGGACTCAGC
  CTGTGAAGCGGCGGGTACTCAGAAAGTGACACTAGTATTTTAAATAAACACCCAAGAAGC
  TTTTGCATCTGATTGTCCCTTCAAGTAGCCTGCTGGGAGCCTAGATCATCAGAGGATGTT
  CATCAGAGGGGGAACAGATCATCCTAGATCACANGAGGAGNTCAGTGCTAAGAATGTGTC
  AAAACCTTCTGTCTGGACCTTCANAGTGNTGACAGGNAGTACTCATTCGTGGCANNATAT
  TATCCGCAGAGGCAGAATCTGAAATTTGGAAACNGNNTCAATATCTTTGGCCACAAGTTN
  NGGTAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002666
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002666.3, NP_002657.2
• RefSeq Size: 2922 bp
• RefSeq ORF: 1569 bp
• Locus ID: 5346
• UniProt ID: O60240
• Cytogenetics: 15q26.1
• Domains: perilipin
• Protein Families: Druggable Genome
• Protein Pathways: PPAR signaling pathway
• Gene Summary: The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Feb 2009]
  Transcript Variant: This variant (1) represents the predominant transcript. Transcript variants 1 and 2 encode the same protein.