PLCG 2 (PLCG2) (NM_002661) Human Untagged Clone

CAT#: SC118495

PLCG2 (untagged)-Human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLCG 2
• Synonyms: APLAID; FCAS3; PLC-gamma-2; PLC-IV
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118495 sequence for NM_002661 edited (data generated by NextGen Sequencing)
  ATGTCCACCACGGTCAATGTAGATTCCCTTGCGGAATATGAGAAGAGCCAGATCAAGAGA
  GCCCTGGAGCTGGGGACGGTGATGACTGTGTTCAGCTTCCGCAAGTCCACCCCCGAGCGG
  AGAACCGTCCAGGTGATCATGGAGACGCGGCAGGTGGCCTGGAGCAAGACCGCCGACAAG
  ATCGAGGGCTTCTTGGATATCATGGAAATAAAAGAAATCCGCCCAGGGAAGAACTCCAAA
  GATTTCGAGCGAGCAAAAGCAGTTCGCCAGAAAGAAGACTGCTGCTTCACCATCCTATAT
  GGCACTCAGTTCGTCCTCAGCACGCTCAGCTTGGCAGCTGACTCTAAAGAGGATGCAGTT
  AACTGGCTCTCTGGCTTGAAAATCTTACACCAGGAAGCGATGAATGCGTCCACGCCCACC
  ATTATCGAGAGTTGGCTGAGAAAGCAGATATATTCTGTGGATCAAACCAGAAGAAACAGC
  ATCAGTCTCCGAGAGTTGAAGACCATCTTGCCCCTGATCAACTTTAAAGTGAGCAGTGCC
  AAGTTCCTTAAAGATAAGTTTGTGGAAATAGGAGCACACAAAGATGAGCTCAGCTTTGAA
  CAGTTCCATCTCTTCTATAAAAAACTTATGTTTGAACAGCAAAAATCGATTCTCGATGAA
  TTCAAAAAGGATTCGTCCGTGTTCATCCTGGGGAACACTGACAGGCCGGATGCCTCTGCT
  GTTTACCTGCATGACTTCCAGAGGTTTCTCATACATGAACAGCAGGAGCATTGGGCTCAG
  GATCTGAACAAAGTCCGTGAGCGGATGACAAAGTTCATTGATGACACCATGCGTGAAACT
  GCTGAGCCTTTCTTGTTTGTGGATGAGTTCCTCACGTACCTGTTTTCACGAGAAAACAGC
  ATCTGGGATGAGAAGTATGACGCGGTGGACATGCAGGACATGAACAACCCCCTGTCTCAT
  TACTGGATCTCCTCGTCACATAACACGTACCTTACAGGTGACCAGCTGCGGAGCGAGTCG
  TCCCCAGAAGCTTACATCCGCTGCCTGCGCATGGGCTGTCGCTGCATTGAACTGGACTGC
  TGGGACGGGCCCGATGGGAAGCCGGTCATCTACCATGGCTGGACGCGGACTACCAAGATC
  AAGTTTGATGACGTCGTGCAGGCCATCAAAGACCACGCCTTTGTTACCTCGAGCTTCCCA
  GTGATCCTGTCCATCGAGGAGCACTGCAGCGTGGAGCAACAGCGTCACATGGCCAAGGCC
  TTCAAGGAAGTATTTGGCGACCTGCTGTTGACGAAGCCCACGGAGGCCAGTGCTGACCAG
  CTGCCCTCGCCCAGCCAGCTGCGGGAGAAGATCATCATCAAGCATAAGAAGCTGGGCCCC
  CGAGGCGATGTGGATGTCAACATGGAGGACAAGAAGGACGAACACAAGCAACAGGGGGAG
  CTGTACATGTGGGATTCCATTGACCAGAAATGGACTCGGCACTACTGCGCCATTGCTGAT
  GCCAAGCTGTCCTTCAGTGATGACATTGAACAGACTATGGAGGAGGAAGTGCCCCAGGAT
  ATACCCCCTACAGAACTACATTTTGGGGAGAAATGGTTCCACAAGAAGGTGGAGAAGAGG
  ACGAGTGCCGAGAAGTTGCTGCAGGAATACTGCATGGAGACGGGGGGCAAGGATGGCACC
  TTCCTGGTTCGGGAGAGCGAGACCTTCCCCAATGACTACACCCTGTCCTTCTGGCGGTCA
  GGCCGGGTCCAGCACTGCCGGATCCGCTCCACCATGGAGGGCGGGACCCTGAAATACTAC
  TTGACTGACAACCTCACCTTCAGCAGCATCTATGCCCTCATCCAGCACTACCGCGAGACG
  CACCTGCGCTGCGCCGAGTTCGAGCTGCGGCTCACGGACCCTGTGCCCAACCCCAACCCC
  CACGAGTCCAAGCCGTGGTACTATGACAGCCTGAGCCGCGGAGAGGCAGAGGACATGCTG
  ATGAGGATTCCCCGGGACGGGGCCTTCCTGATCCGGAAGCGAGAGGGGAGCGACTCCTAT
  GCCATCACCTTCAGGGCTAGGGGCAAGGTAAAGCATTGTCGCATCAACCGGGACGGCCGG
  CACTTTGTGCTGGGGACCTCCGCCTATTTTGAGAGTCTGGTGGAGCTCGTCAGTTACTAC
  GAGAAGCATTCACTCTACCGAAAGATGAGACTGCGCTACCCCGTGACCCCCGAGCTCCTG
  GAGCGCTACAATATGGAAAGAGATATAAACTCCCTCTACGACGTCAGCAGAATGTATGTG
  GATCCCAGTGAAATCAATCCGTCCATGCCTCAGAGAACCGTGAAAGCTCTGTATGACTAC
  AAAGCCAAGCGAAGCGATGAGCTGAGCTTCTGCCGTGGTGCCCTCATCCACAATGTCTCC
  AAGGAGCCCGGGGGCTGGTGGAAAGGAGACTATGGAACCAGGATCCAGCAGTACTTCCCA
  TCCAACTACGTCGAGGACATCTCAACTGCAGACTTCGAGGAGCTAGAAAAGCAGATTATT
  GAAGACAATCCCTTAGGGTCTCTTTGCAGAGGAATATTGGACCTCAATACCTATAACGTC
  GTGAAAGCCCCTCAGGGAAAAAACCAGAAGTCCTTTGTCTTCATCCTGGAGCCCAAGCAG
  CAGGGCGATCCTCCGGTGGAGTTTGCCACAGACAGGGTGGAGGAGCTCTTTGAGTGGTTT
  CAGAGCATCCGAGAGATCACCTGGAAGATTGACACCAAGGAGAACAACATGAAGTACTGG
  GAGAAGAACCAGTCCATCGCCATCGAGCTCTCTGACCTGGTTGTCTACTGCAAACCAACC
  AGCAAAACCAAGGACAACTTAGAAAATCCTGACTTCCGAGAAATCCGCTCCTTTGTGGAG
  ACGAAGGCTGACAGCATCATCAGACAGAAGCCCGTCGACCTCCTGAAGTACAATCAAAAG
  GGCCTGACCCGCGTCTACCCAAAGGGACAAAGAGTTGACTCTTCAAACTACGACCCCTTC
  CGCCTCTGGCTGTGCGGTTCTCAGATGGTGGCACTCAATTTCCAGACGGCAGATAAGTAC
  ATGCAGATGAATCACGCATTGTTTTCTCTCAATGGGCGCACGGGCTACGTTCTGCAGCCT
  GAGAGCATGAGGACAGAGAAATATGACCCGATGCCACCCGAGTCCCAGAGGAAGATCCTG
  ATGACGCTGACAGTCAAGGTTCTCGGTGCTCGCCATCTCCCCAAACTTGGACGAAGTATT
  GCCTGTCCCTTTGTAGAAGTGGAGATCTGTGGAGCCGAGTATGACAACAACAAGTTCAAG
  ACGACGGTTGTGAATGATAATGGCCTCAGCCCTATCTGGGCTCCAACACAGGAGAAGGTG
  ACATTTGAAATTTATGACCCAAACCTGGCATTTCTGCGCTTTGTGGTTTATGAAGAAGAT
  ATGTTCAGCGATCCCAACTTTCTTGCTCATGCCACTTACCCCATTAAAGCAGTCAAATCA
  GGATTCAGGTCCGTTCCTCTGAAGAATGGGTACAGCGAGGACATAGAGCTGGCTTCCCTC
  CTGGTTTTCTGTGAGATGCGGCCAGTCCTGGAGAGCGAAGAGGAACTTTACTCCTCCTGT
  CGCCAGCTGAGGAGGCGGCAAGAAGAACTGAACAACCAGCTCTTTCTGTATGACACACAC
  CAGAACTTGCGCAATGCCAACCGGGATGCCCTGGTTAAAGAGTTCAGTGTTAATGAGAAC
  CAGCTCCAGCTGTACCAGGAGAAATGCAACAAGAGGTTAAGAGAGAAGAGAGTCAGCAAC
  AGCAAGTTTTACTCATAG
  
  Clone variation with respect to NM_002661.3
  1149 c=>t;1497 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002661 unedited
  TTACCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC
  AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG
  AATTCGGCACGAGGCCGATTCCTTCCTTCTCCCTGGAGCGGCCGACAATGTCCACCACGG
  TCAATGTAGATTCCCTTGCGGAATATGAGAAGAGCCAGATCAAGAGAGCCCTGGAGCTGG
  GGACGGTGATGACTGTGTTCAGCTTCCGCAAGTCCACCCCCGAGCGGAGAACCGTCCAGG
  TGATCATGGAGACGCGGCAGGTGGCCTGGAGCAAGACCGCCGACAAGATCGAGGGCTTCT
  TGGATATCATGGAAATAAAAGAAATCCGCCCAGGGAAGAACTCCAAAGATTTCGAGCGAG
  CAAAAGCAGTTCGCCAGAAAGAAGACTGCTGCTTCACCATCCTATATGGCACTCAGTTCG
  TCCTCAGCACGCTCAGCTTGGCAGCTGACTCTAAAGAGGATGCAGTTAACTGGCTCTCTG
  GCTTGAAAATCTTACACCAGGAAGCGATGAATGCGTCCACGCCCACCATTATCGAGAGTT
  GGCTGAGAAAGCAGATATATTCTGTGGATCAAACCAGAAGAAACAGCATCAGTCTCCGAG
  AGTTGAAGACCATCTTGCCCCTGATCAACTNTAAAGTGAGCAGTGCCAAGTTCCTTAAAG
  ATAAGTTTGTGGAAATAGGAGCACACCAAAGATGAGCTCAGCTTGAACAGTTCCATCTCT
  TCTATAAAAAACTATGTTTGAACAGCANAATCGATTCTCGATGAATTCANAAGGATNCGT
  CCGTGNTCATCCTGNGGGACACTGACAGGCCGNATGCTCTGCTGTTAACTGCATGACTNN
  CAGAGGNTNCTCATACATGACAGCCAGAGCATGGGCTTCAGATCTGACA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002661 unedited
  NNGGGTATTTTATGNACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTCAA
  GTTTTCATTGCTTTTATTCAATGAGGTTTGGAACATGAGAGGCCAAAAATGAGAGCACAT
  TTTGGGGAATCCTCTATAAAGAATATAAGTTAAGAAAGTTGTCCAAGATGAAAATAATAG
  GAAATTACCCCACCTCATGCCAGTTGGGTATAAAAAAATGTCCTTGATGGCAGGCTTGAA
  GAAAAGATGTCACAGATTAGCGTCTTCCCAGAGTGTGAATAGGGCACGTTCTCCTACATG
  CAAACACACATGCGCACACACACAATACCCTTACACACATACCCCAGCTTCTATGAGTAA
  AACTTGCTGTTGCTGACTCTCTTCTCTCTTAACCTCTTGTTGCATTTCTCCTGGTACAGC
  TGGAGCTGGTTCTCATTAACACTGAACTCTTTAACCAGGGCATCCCGGTTGGCATTGCGC
  AAGTTCTGGTGTGTGTCATACAGAAAGAGCTGGTTGTTCAGTTCTTCTTGCCGCCTCCTC
  AGCTGGCGACAGGAGGAGTAAAGTTCCTCTTCGCTCTCCAGGACTGGCCGCATCTCACAG
  AAAACCAGGAGGGAAGCCAGCTCTATGTCCTCGCTGTACCCATTCTTCAGAGGAACGGAC
  CTGAATCCTGATTTGACTGCTTTAATGGGGTAAGTGGCATGAGCAAGAAAGTTGGGATCG
  CTGAACATATCTTCTTCATAAACCACAAAGCGCAGAAATGCCAGGTTTGGGTCATAAATT
  TCAAATGTCACCTTCTCCTGTGTTGGAGCCCAGATAGGGCTGAGGCCATTATCATTCACA
  ACCGTCGTCTTGAACTTGTTGTTGTCATACTCGGGCTCACAGATCTCCACTTCTACAAGG
  GACAGGCATN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002661
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002661.1, NP_002652.1
• RefSeq Size: 4242 bp
• RefSeq ORF: 3759 bp
• Locus ID: 5336
• UniProt ID: P16885
• Cytogenetics: 16q23.3
• Domains: C2, PI-PLC-X, SH2, SH3, PI-PLC-Y, PH
• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway, Calcium signaling pathway, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Glioma, Inositol phosphate metabolism, Leukocyte transendothelial migration, Metabolic pathways, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, VEGF signaling pathway, Vibrio cholerae infection
• Gene Summary: The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. [provided by RefSeq, Mar 2014]