uPA (PLAU) (NM_002658) Human Untagged Clone

CAT#: SC118493

PLAU (untagged)-Human plasminogen activator, urokinase (PLAU), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: uPA
• Synonyms: ATF; BDPLT5; QPD; u-PA; UPA; URK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002658 edited
  GAATTCGGCACGAGGGCAGCCCCGGAGCCCGGGCCAGGGTCCACCTGTCCCCGCAGCGCC
  GGCTCGCGCCCTCCTGCCGCAGCCACCGAGCCGCCGTCTAGCGCCCCGACCTCGCCACCA
  TGAGAGCCCTGCTGGCGCGCCTGCTTCTCTGCGTCCTGGTCGTGAGCGACTCCAAAGGCA
  GCAATGAACTTCATCAAGTTCCATCGAACTGTGACTGTCTAAATGGAGGAACATGTGTGT
  CCAACAAGTACTTCTCCAACATTCACTGGTGCAACTGCCCAAAGAAATTCGGAGGGCAGC
  ACTGTGAAATAGATAAGTCAAAAACCTGCTATGAGGGGAATGGTCACTTTTACCGAGGAA
  AGGCCAGCACTGACACCATGGGCCGGCCCTGCCTGCCCTGGAACTCTGCCACTGTCCTTC
  AGCAAACGTACCATGCCCACAGATCTGATGCTCTTCAGCTGGGCCTGGGGAAACATAATT
  ACTGCAGGAACCCAGACAACCGGAGGCGACCCTGGTGCTATGTGCAGGTGGGCCTAAAGC
  CGCTTGTCCAAGAGTGCATGGTGCATGACTGCGCAGATGGAAAAAAGCCCTCCTCTCCTC
  CAGAAGAATTAAAATTTCAGTGTGGCCAAAAGACTCTGAGGCCCCGCTTTAAGATTATTG
  GGGGAGAATTCACCACCATCGAGAACCAGCCCTGGTTTGCGGCCATCTACAGGAGGCACC
  GGGGGGGCTCTGTCACCTACGTGTGTGGAGGCAGCCTCATCAGCCCTTGCTGGGTGATCA
  GCGCCACACACTGCTTCATTGATTACCCAAAGAAGGAGGACTACATCGTCTACCTGGGTC
  GCTCAAGGCTTAACTCCAACACGCAAGGGGAGATGAAGTTTGAGGTGGAAAACCTCATCC
  TACACAAGGACTACAGCGCTGACACGCTTGCTCACCACAACGACATTGCCTTGCTGAAGA
  TCCGTTCCAAGGAGGGCAGGTGTGCGCAGCCATCCCGGACTATACAGACCATCTGCCTGC
  CCTCGATGTATAACGATCCCCAGTTTGGCACAAGCTGTGAGATCACTGGCTTTGGAAAAG
  AGAATTCTACCGACTATCTCTATCCGGAGCAGCTGAAAATGACTGTTGTGAAGCTGATTT
  CCCACCGGGAGTGTCAGCAGCCCCACTACTACGGCTCTGAAGTCACCACCAAAATGCTGT
  GTGCTGCTGACCCACAGTGGAAAACAGATTCCTGCCAGGGAGACTCAGGGGGACCCCTCG
  TCTGTTCCCTCCAAGGCCGCATGACTTTGACTGGAATTGTGAGCTGGGGCCGTGGATGTG
  CCCTGAAGGACAAGCCAGGCGTCTACACGAGAGTCTCACACTTCTTACCCTGGATCCGCA
  GTCACACCAAGGAAGAGAATGGCCTGGCCCTCTGAGGGTCCCCAGGGAGGAAACGGGCAC
  CACCCGCTTTCTTGCTGGTTGTCATTTTTGCAGTAGAGTCATCTCCATCAGCTGTAAGAA
  GAGACTGGGAAGATAGGCTCTGCACAGATGGATTTGCCTGTGCCACCCACCAGGGTGAAC
  GACAATAGCTTTACCCTCAGGCATAGGCCTGGGTGCTGGCTGCCCAGACCCCTCTGGCCA
  GGATGGAGGGGTGGTCCTGACTCAACATGTTACTGACCAGCAACTTGTCTTTTTCTGGAC
  TGAAGCCTGCAGGAGTTAAAAAGGGCAGGGCATCTCCTGTGCATGGGTGAAGGGAGAGCC
  AGCTCCCCCGACGGTGGGCATTTGTGAGGCCCATGGTTGAGAAATGAATAATTTCCCAAT
  TAGGAAGTGTAACAGCTGAGGTCTCTTGAGGGAGCTTAGCCAATGTGGGAGCAGCGGTTT
  GGGGAGCAGAGACACTAACGACTTCAGGGCAGGGCTCTGATATTCCATGAATGTATCAGG
  AAATATATATGTGTGTGTATGTTTGCACACTTGTGTGTGGGCTGTGAGTGTAAGTGTGAG
  TAAGAGCTGGTGTCTGATTGTTAAGTCTAAATATTTCCTTAAACTGTGTGGACTGTGATG
  CCACACAGAGTGGTCTTTCTGGAGAGGTTATAGGTCACTCCTGGGGCCTCTTGGGTCCCC
  CACGTGACAGTGCCTGGGAATGTATTATTCTGCAGCATGACCTGTGACCAGCACTGTCTC
  AGTTTCACTTTCACATAGATGTCCCTTTCTTGGCCAGTTATCCCTTCCTTTTAGCCTAGT
  TCATCCAATCCTCACTGGGTGGGGTGAGGACCACTCCTTACACTGAATATTTATATTTCA
  CTATTTTTATTTATATTTTTGTAATTTTAAATAAAAGTGATCAATAAXXXXXXXXXXXXX
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002658 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAGCCCCGGAGCCC
  GGGCCAGGGTCCACCTGTCCCCGCAGCGCCGGCTCGCGCCCTCCTGCCGCAGCCACCGAG
  CCGCCGTCTAGCGCCCCGACCTCGCCACCATGAGAGCCCTGCTGGCGCGCCTGCTTCTCT
  GCGTCCTGGTCGTGAGCGACTCCAAAGGCAGCAATGAACTTCATCAAGTTCCATCGAACT
  GTGACTGTCTAAATGGAGGAACATGTGTGTCCAACAAGTACTTCTCCAACATTCACTGGT
  GCAACTGCCCAAAGAAATTCGGAGGGCAGCACTGTGAAATAGATAAGTCAAAAACCTGCT
  ATGAGGGGAATGGTCACTTTTACCGAGGAAAGGCCAGCACTGACACCATGGGCCGGCCCT
  GCCTGCCCTGGAACTCTGCCACTGTCCTTCAGCAAACGTACCATGCCCACAGATCTGATG
  CTCTTCAGCTGGGCCTGGGGAAACATAATTACTGCAGGAACCCAGACAACCGGAGGCGAC
  CCTGGTGCTATGTGCAGGTGGGCCTAAAGCCGCTTGTCCAAGAGTGCATGGTGCATGACT
  GCGCAGATGGAAAAAAGCCCTCCTCTCCTCCAGAAGAATTAANATTTCAGTGTGGCCAAA
  AGACTCTGACGCCCCCGCTNTAAGAATATTGGGGGAGAATTCACCACCATCGAGAACCAG
  CCCTGGTTTGCGGNCATCTACAGGAGGCACCGGGNGGGGCTCTGTCACCTACGTGTGTGG
  AGGCAGCCTCATCAGCCCTTGCTGGGTGATCAGCGGCACACACTGCTTCATTGATTACCC
  CAAGAGGAGGACTACATCGTCTACCTGGGTCGCTCAAGGCTTACTCCACACGCAAGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002658 unedited
  GGAGCGCGCGCGCCCCACCACNAAACACACCCCCNNNNNANNNGNATTTTTCGTNAAAAT
  ACACATTTTNTATGNTNCACNNTTTTATTTAAAATTACCAAAAATATTAAANNNAAAAAT
  AGTGAAATATAAATATTCAGTGTAAGGAGTGGTCCTCACCCCACCCAGTGAGGATTGGAT
  GAACTAGGCTAAAAGGAAGGGATAACTGGCCAAGAAAGGGACATCTATGTGAAAGTGAAA
  CTGAGACAGTGCTGGTCACAGGTCATGCTGCAGAATAATACATTCCCAGGCACTGTCACG
  TGGGGGACCCAAGAGGCCCCAGGAGTGACCTATAACCTCTCCAGAAAGACCACTCTGTGT
  GGCATCACAGTCCACACAGTTTAAGGAAATATTTAGACTTAACAATCAGACACCAGCTCT
  TACTCACACTTACACTCACAGCCCACACACAAGTGTGCAAACATACACACACATATATAT
  TTCCTGATACATTCATGGAATATCAGAGCCCTGCCCTGAAGTCGTTAGTGTCTCTGCTCC
  CCAAACCGCTGCTCCCACATTGGCTAAGCTCCCTCAAGAGACCTCAGCTGTTACACTTCC
  TAATTGGGAAATTATTCATTTCTCAACCATGGGCCTCACAAATGCCCACCGTCGGGGGAG
  CTGGCTCTCCCTTCACCCATGCACAGGAGATGCCCTGCCCTTTTTAACTCCTGCAAGCTT
  CAGTCCAGAAAAAGACAAGTTGCTGGTCAGTAACATGTTGAGTCAAGACCACCCCTCCAT
  CCTGGCCAGAGGGGTCTTGGGCAGCCAGCACCCAGGCCTATGCCTGAAGGTAAAGCTATT
  GTCGTTCACCCTGGTGGGTGGGCACAGGCAAATCCATTTGTGGCAAACCCTATCTTCCAA
  ATCTCTTCTTTACAACTGATGGAGTAGACTCTTCTGCAAAAATGACCACCACCAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002658
• Insert Size: 2560 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002658.2, NP_002649.1
• RefSeq Size: 2360 bp
• RefSeq ORF: 1296 bp
• Locus ID: 5328
• UniProt ID: P00749
• Cytogenetics: 10q22.2
• Domains: KR, Tryp_SPc
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protease
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: This gene encodes a secreted serine protease that converts plasminogen to plasmin. The encoded preproprotein is proteolytically processed to generate A and B polypeptide chains. These chains associate via a single disulfide bond to form the catalytically inactive high molecular weight urokinase-type plasminogen activator (HMW-uPA). HMW-uPA can be further processed into the catalytically active low molecular weight urokinase-type plasminogen activator (LMW-uPA). This low molecular weight form does not bind to the urokinase-type plasminogen activator receptor. Mutations in this gene may be associated with Quebec platelet disorder and late-onset Alzheimer's disease. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) encodes the longest isoform (1).