PSMD7 (NM_002811) Human Untagged Clone

CAT#: SC118382

PSMD7 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (PSMD7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMD7
• Synonyms: MOV34; P40; Rpn8; S12
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118382 sequence for NM_002811 edited (data generated by NextGen Sequencing)
  ATGCCGGAGCTGGCAGTGCAGAAGGTGGTGGTCCACCCCCTGGTGCTGCTCAGTGTGGTG
  GATCATTTCAACCGAATCGGCAAGGTTGGAAACCAGAAGCGTGTTGTTGGTGTGCTTTTG
  GGGTCATGGCAAAAGAAAGTACTTGATGTATCGAACAGTTTTGCAGTTCCTTTTGATGAA
  GATGACAAAGACGATTCTGTATGGTTTTTAGACCATGATTATTTGGAAAACATGTATGGA
  ATGTTTAAGAAAGTCAATGCCAGGGAAAGAATAGTTGGCTGGTACCACACAGGCCCTAAA
  CTACACAAGAATGACATTGCCATCAACGAACTCATGAAAAGATACTGTCCTAATTCCGTA
  TTGGTCATCATTGATGTGAAGCCGAAGGACCTAGGGCTGCCTACAGAAGCGTACATTTCA
  GTGGAAGAAGTCCATGATGATGGAACTCCAACCTCGAAAACATTTGAACACGTGACCAGT
  GAAATTGGAGCAGAGGAAGCTGAGGAAGTTGGAGTTGAACACTTGTTACGAGATATCAAA
  GACACGACGGTGGGCACTCTGTCCCAGCGGATCACAAACCAGGTCCATGGTTTGAAGGGA
  CTGAACTCCAAGCTTCTGGATATCAGGAGCTACCTGGAAAAAGTCGCCACAGGCAAGCTG
  CCCATCAACCACCAGATCATCTACCAGCTGCAGGACGTCTTCAACCTGCTGCCAGATGTC
  AGCCTGCAGGAGTTCGTCAAGGCCTTTTACCTGAAGACCAATGACCAGATGGTGGTAGTG
  TACTTGGCCTCGCTGATCCGTTCCGTGGTCGCCCTGCACAACCTCATCAACAACAAGATT
  GCCAACCGGGATGCAGAGAAGAAAGAAGGGCAGGAGAAAGAAGAGAGCAAAAAGGATAGG
  AAAGAGGACAAGGAGAAAGATAAAGATAAGGAAAAGAGTGATGTAAAGAAAGAGGAGAAA
  AAGGAGAAAAAGTAA
  
  Clone variation with respect to NM_002811.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002811 unedited
  TCGGATTTTGTAATACGACTTACTATTAGGGCGGCCGCGAATTCGCACCAGGGTGACCGC
  TACTGCTGCCGGTGTTTGCGTGTGGCAGGGAGCCAGGCCTGGCGAGCGGGGTGTGTCGCG
  ATGCCGGAGCTGGCAGTGCAGAAGGTGGTGGTCCACCCCCTGGTGCTGCTCAGTGTGGTG
  GATCATTTCAACCGAATCGGCAAGGTTGGAAACCAGAAGCGTGTTGTTGGTGTGCTTTTG
  GGGTCATGGCAAAAGAAAGTACTTGATGTATCGAACAGTTTTGCAGTTCCTTTTGATGAA
  GATGACAAAGACGATTCTGTATGGTTTTTAGACCATGATTATTTGGAAAACATGTATGGA
  ATGTTTAAGAAAGTCAATGCCAGGGAAAGAATAGTTGGCTGGTACCACACAGGCCCTAAA
  CTACACAAGAATGACATTGCCATCAACGAACTCATGAAAAGATACTGTCCTAATTCCGTA
  TTGGTCATCATTGATGTGAAGCCGAAGGACCTAGGGCTGCCTACAGAAGCGTACATTTCA
  GTGGAAGAAGTCCATGATGATGGAACTCCAACCTCGAAAACATTTGAACACGTGACCAGT
  GAAATTGGAGCAGAGGAAGCTGAGGAAGTTGGAGTTGAACACTTGTTACGAGATATCAAA
  GACACGACGGTGGGCACTCTGTCCCAGCGGATCACAAACCAGGTCCATGGTTTGAAGGGA
  CTGAACTNCAGCTTCTGGATATCANGAGCTACCNTGNAAAAGTCGCCACAGGCAAGCTGC
  CCATCAACCACCAGATCATCTACCAGCTGCAGGACGTCNTCAACCTGCTGCCAGATGTCA
  GCCTGCNAGAGTTCGTCAAGGCCCTTTACCTGNAGACCATGACCAGTGGGGGTAGTGTAC
  CTGGCCTCGCTGATCCGTTCGTGGTCGCCTGNCCAACTCATAACAACAGATGCCAACCGG
  ATGCAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002811
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002811.3, NP_002802.2
• RefSeq Size: 1678 bp
• RefSeq ORF: 975 bp
• Locus ID: 5713
• UniProt ID: P51665
• Cytogenetics: 16q23.1
• Domains: JAB_MPN
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 17. [provided by RefSeq, Jul 2008]