RPA34 (RPA2) (NM_002946) Human Untagged Clone

CAT#: SC118290

RPA2 (untagged)-Human replication protein A2, 32kDa (RPA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPA34
• Synonyms: REPA2; RP-A p32; RP-A p34; RPA32
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118290 sequence for NM_002946 edited (data generated by NextGen Sequencing)
  ATGTGGAACAGTGGATTCGAAAGCTATGGCAGCTCCTCATACGGGGGAGCCGGCGGCTAC
  ACGCAGTCCCCGGGGGGCTTTGGATCGCCCGCACCTTCTCAAGCCGAAAAGAAATCAAGA
  GCCCGAGCCCAGCACATTGTGCCCTGTACTATATCTCAGCTGCTTTCTGCCACTTTGGTT
  GATGAAGTGTTCAGAATTGGGAATGTTGAGATTTCACAGGTCACTATTGTGGGGATCATC
  AGACATGCAGAGAAGGCTCCAACCAACATTGTTTACAAAATAGATGACATGACAGCTGCA
  CCCATGGACGTTCGCCAGTGGGTTGACACAGATGACACCAGCAGTGAAAACACTGTGGTT
  CCTCCAGAAACATATGTGAAAGTGGCAGGCCACCTGAGATCTTTTCAGAACAAAAAGAGC
  CTGGTAGCCTTTAAGATCATGCCCCTGGAGGATATGAATGAGTTCACCACACATATTCTG
  GAAGTGATCAATGCACACATGGTACTAAGCAAAGCCAACAGCCAGCCCTCAGCAGGGAGA
  GCACCTATCAGCAATCCAGGAATGAGTGAAGCAGGGAACTTTGGTGGGAATAGCTTCATG
  CCAGCAAATGGCCTCACTGTGGCCCAAAACCAGGTGTTGAATTTGATTAAGGCTTGTCCA
  AGACCTGAAGGGTTGAACTTTCAGGATCTCAAGAACCAGCTGAAACACATGTCTGTATCC
  TCAATCAAGCAAGCTGTGGATTTTCTGAGCAATGAGGGGCACATCTATTCTACTGTGGAT
  GATGACCATTTTAAATCCACAGATGCAGAATAA
  
  Clone variation with respect to NM_002946.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002946 unedited
  TGTCGNAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGTGGCGGCCG
  CGTTCTGTGGTTTTCCGCTATTCCCCCANACCCGCACCTTCTCGGCCTCTTTGCGGAGAA
  TCGTGACCAAGATGTGGAACAGTGGATTCGAAAGCTATGGCAGCTCCTCATACGGGGGAG
  CCGGCGGCTACACGCAGTCCCCGGGGGGCTTTGGATCGCCCGCACCTTCTCAAGCCGAAA
  AGAAATCAAGAGCCCGAGCCCAGCACATTGTGCCCTGTACTATATCTCAGCTGCTTTCTG
  CCACTTTGGTTGATGAAGTGTTCAGAATTGGGAATGTTGAGATTTCACAGGTCACTATTG
  TGGGGATCATCAGACATGCAGAGAAGGCTCCAACCAACATTGTTTACAAAATAGATGACA
  TGACAGCTGCACCCATGGACGTTCGCCAGTGGGTTGACACAGATGACACCAGCAGTGAAA
  ACACTGTGGTTCCTCCAGAAACATATGTGAAAGTGGCAGGCCACCTGAGATCTTTTCAGA
  ACAAAAAGAGCCTGGTAGCCTTTAAGATCATGCCCCTGGAGGATATGAATGAGTTCACCA
  CACATATTCTGGAAGTGATCAATGCACACATGGTACTAAGCAAAGCCAACAGCCAGCCCT
  CAGCAGGGAGAGCACCTATCAGCAATCCAGGAATGAGTGAAGCAGGGAACTTTGGTGGGA
  ATAGCTTCATGCCAGCANATGGCCTCACTGTGGCCCANNACCAGTGTTGAAATTGATTAA
  GGCTTGTCCAAGACCTGAAGGGTTGAACTTTCAGGGATCTCAGACCAGCTGAACACATGT
  CTGTATCCTCATCAAGCAGCTGTGGATTTTTGAGCATGAGGGCACATCTATTTACTGTGG
  ATGATGACCATTTAATA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002946 unedited
  CTTGTACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTACAAAAAAACTGTTTTATTAAACATATGACTGTATTTATTTTGTACA
  AAATGCAGTAACACTTTTTCTTTTCCTCTGCCCCTGGGAGGCACTACCGCAAAACTTCAT
  GTTCACAAAAACACACGTCATGGGAAGTGTGTCAAAAAGTCATGACAAGCAGGAAAGGGC
  AATTTTGAAAGCAATGCCCCACAACCAAAGTGGGAAATGAAAACCGGCAGAGGCCAACAG
  GTTTCTTGGCTAGGGCTCTGCCTATAACGCCCTGGACCTGCTCATTTTTCAGCAACAAAC
  ATTTTAAGAAAAAAAACTCCTGAGCACTATGTAAGTCCCTTCCTGGTACCATCCTTACAA
  TTCCATCTATTTAGAGGGTGAAACAAGCTTATTTTTCACTTCCTCAACAAACCCAAATCC
  GTATTGCAACCCTCTCCAATTCCCCACTTCCCTTTGATTTTTATACTATCACCCACATAC
  CCCAGCACAACATTCTATTTTCACAGGTACTTTAAGCGCCAATTTCTTAACCCACCACTC
  ATTGCCAAAAAACCCTTGGCCAAACTTATTCATACCTCGACACCACACCCTTCCACATCT
  ACCCCCCTCACTTACACTACTTCTTGTCCTCAAAATATTACCCTCCTCTTACTCTCTCTC
  GTGCTCTAAAACTGGCCCCCCTCTCACCTCTTATTACTTCTTTCCTTCCACTCCCCCCTC
  TCCCCCACCCTCCCTCCCCCGCAGTGTACCCCCACTCTTTTTCACTCCAGCCCCCCCCAC
  CCCCGACACCCCCACCCCCCCGCCCCCCTCCCTCCCCCCCACCACCTGCAACTCCGCTCC
  CCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002946
• Insert Size: 1630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002946.3, NP_002937.1
• RefSeq Size: 1741 bp
• RefSeq ORF: 813 bp
• Locus ID: 6118
• UniProt ID: P15927
• Cytogenetics: 1p35.3
• Domains: tRNA_anti
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: DNA replication, Homologous recombination, Mismatch repair, Nucleotide excision repair
• Gene Summary: This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]
  Transcript Variant: This variant (1) encodes isoform 1.