RPA70 (RPA1) (NM_002945) Human Untagged Clone

CAT#: SC118289

RPA1 (untagged)-Human replication protein A1, 70kDa (RPA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPA70
• Synonyms: HSSB; MST075; REPA1; RF-A; RP-A; RPA70
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118289 sequence for NM_002945 edited (data generated by NextGen Sequencing)
  ATGGTCGGCCAGCTGAGCGAGGGGGCCATTGCGGCCATCATGCAGAAGGGGGATACAAAC
  ATAAAGCCCATCCTCCAAGTCATCAACATCCGTCCCATTACTACGGGGAATAGTCCGCCG
  CGTTATCGACTGCTCATGAGTGATGGATTGAACACTCTATCCTCTTTCATGTTGGCGACA
  CAGTTGAACCCTCTCGTGGAGGAAGAACAATTGTCCAGCAACTGTGTATGCCAGATTCAC
  AGATTTATTGTGAACACTCTGAAAGACGGAAGGAGAGTAGTTATCTTGATGGAATTAGAA
  GTTTTGAAGTCAGCTGAAGCAGTTGGAGTGAAGATTGGCAATCCAGTGCCCTATAATGAA
  GGACTCGGGCAGCCGCAAGTAGCTCCTCCAGCGCCAGCAGCCAGCCCAGCAGCAAGCAGC
  AGGCCCCAGCCGCAGAATGGAAGCTCGGGAATGGGTTCTACTGTTTCTAAGGCTTATGGT
  GCTTCAAAGACATTTGGAAAAGCTGCAGGTCCCAGCCTGTCACACACTTCTGGGGGAACA
  CAGTCCAAAGTGGTGCCCATTGCCAGCCTCACTCCTTACCAGTCCAAGTGGACCATTTGT
  GCTCGTGTTACCAACAAAAGTCAGATCCGTACCTGGAGCAACTCCCGAGGGGAAGGGAAG
  CTTTTCTCCCTAGAACTGGTTGACGAAAGTGGTGAAATCCGAGCTACAGCTTTCAATGAG
  CAAGTGGACAAGTTCTTTCCTCTTATTGAAGTGAACAAGGTGTATTATTTCTCGAAAGGC
  ACCCTGAAGATTGCTAACAAGCAGTTCACAGCTGTTAAAAATGACTACGAGATGACCTTC
  AATAACGAGACTTCCGTCATGCCCTGTGAGGACGACCATCATTTACCTACGGTTCAGTTT
  GATTTCACGGGGATTGATGACCTCGAGAACAAGTCGAAAGACTCACTTGTAGACATCATC
  GGGATCTGCAAGAGCTATGAAGACGCCACTAAAATCACAGTGAGGTCTAACAACAGAGAA
  GTTGCCAAGAGGAATATCTACTTGATGGACACATCCGGGAAGGTGGTGACTGCTACACTG
  TGGGGGGAAGATGCTGATAAATTTGATGGTTCTAGACAGCCCGTGTTGGCTATCAAAGGA
  GCCCGAGTCTCTGATTTCGGTGGACGGAGCCTCTCCGTGCTGTCTTCAAGCACTATCATT
  GCGAATCCTGACATCCCAGAGGCCTATAAGCTTCGTGGATGGTTTGACGCAGAAGGACAA
  GCCTTAGATGGTGTTTCCATCTCTGATCTAAAGAGCGGCGGAGTCGGAGGGAGTAACACC
  AACTGGAAAACCTTGTATGAGGTCAAATCCGAGAACCTGGGCCAAGGCGACAAGCCGGAC
  TACTTTAGTTCTGTGGCCACAGTGGTGTATCTTCGCAAAGAGAACTGCATGTACCAAGCC
  TGCCCGACTCAGGACTGCAATAAGAAAGTGATTGATCAACAGAATGGATTGTACCGCTGT
  GAGAAGTGCGACACCGAATTTCCCAATTTCAAGTACCGCATGATCCTGTCAGTAAATATT
  GCAGATTTTCAAGAGAATCAGTGGGTGACTTGTTTCCAGGAGTCTGCTGAAGCTATCCTT
  GGACAAAATGCTGCTTATCTTGGGGAATTAAAAGACAAGAATGAACAGGCATTTGAAGAA
  GTTTTCCAGAATGCCAACTTCCGATCTTTCATATTCAGAGTCAGGGTCAAAGTGGAGACC
  TACAACGACGAGTCTCGAATTAAGGCCACTGTGATGGACGTGAAGCCCGTGGACTACAGA
  GAGTATGGCCGAAGGCTGGTCATGAGCATCAGGAGAAGTGCATTGATGTGA
  
  Clone variation with respect to NM_002945.3
  12 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002945 unedited
  TATACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGGGGACCCGGGTGGGGAAGCTG
  GAGCTGTTGCGGGGTCCGCGGGGAAGTCTTGGCGGTGGAGCCATGGTCGGCCAGCTGAGC
  GAGGGGGCCATTGCGGCCATCATGCAGAAGGGGGATACAAACATAAAGCCCATCCTCCAA
  GTCATCAACATCCGTCCCATTACTACGGGGAATAGTCCGCCGCGTTATCGACTGCTCATG
  AGTGATGGATTGAACACTCTATCCTCTTTCATGTTGGCGACACAGTTGAACCCTCTCGTG
  GAGGAAGAACAATTGTCCAGCAACTGTGTATGCCAGATTCACAGATTTATTGTGAACACT
  CTGAAAGACGGAAGGAGAGTAGTTATCTTGATGGATTTAGAAGTTNTGAAGTCAGCTGAA
  GCAGTTGGAGTGNAAGATTGGCAATCCAGTGCCCTATAATGAAGGACTCGGGCAGCCGCA
  AGTAGCTCCTCCAGCGCCAGCAGCCAGCCCAGCAGCAAGCAGCAGGCCCCAGCCGCAGAA
  TGGAAGCTGGGNAAATGGGTTCTACTGTTTCTAGGGCTTATGGTGCTTCAAAGACATTTG
  GAAAGGCTGCAGGTCCCAGCCTGTCACACACTTCTGGNGGAACACAGTCNAAAGTGGTGC
  CCATTGCCAGCCTCACTCCTTACCAGTCCAAAGTGACCCATTTGTGGCTCGTGTTACCAA
  CANAAGTCAAAATCCGTACCTGGAGCACTNCCGAAGGGGAAAAGGGAAAGCTTTTCTCCC
  TAGAACCTGNTGACAAAAAGTGGTGGAAATCCGAGCTACAGCTTTTCAATGAGCAGTGGG
  ACCAAGTTCTTTTCTCTTTTTTGGAGTGAACCAGGGGTGATTATTTTTTCGAAAGGCACC
  CTTGAGAATGCAAACAAGCGTTCACCAGCTGTTAAAATGACTACCAAATGACCTCATACG
  AAACTTCGTCTGCCTTGGAAGACACATATTACTCCGCGGTTAATTGATCTCCGGGGGAAA
  GA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002945 unedited
  GGACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACAACCAAAGATATA
  CTGTTTATTAAAACAAAGCAAGGGAAAACAAAAGGCTTCAAAATCCTTTCCATGTAACAT
  AGCTTTAGTCAAAAAAAACAAAATACATGAATGCAGGAATGTGTACAGGTAAAAAAAGTA
  CGCACTCACCAAGCGAAGCGCAGCATTAATTGAAGTGAAAAGTAAAGAAACGCTTTGTAG
  CCGCTCGGATTTCCTTAGGGGACAGCAAGTCAATGCTTATCAGCGGTCCTCAAAAGAGAC
  AATTTTGATTAATATCAGACCCATCTGACTCAGTCTATTAAACCCTGAAGGAAGGATACT
  CTTCAGATATAGAAGATATGCCTTTGATTAATCATTCTACCCTATTGCCATTCCAAGCAT
  TACCAACTATGTAGCACCTCTAGAAAGAGAGAGCCTTCGAATTCTAGGAAGACACACTTA
  CTAAAATCCCCAGGTGCTCATGACCGCTTCTCAGCTCGCCCCGCTGAGAAGAACCCAAGT
  TGTGAGCGGGATGGNGGGGGGGTTGTGNACACCACTGGAGAAGCAAAAACCTAGTTACAT
  AATTTACTTCATGGTCTGCAGTTAGGGTCAGTGACTTACGACATAATTCCTGCTTGATGA
  TAATGAAATTGACAGAAGNCCTGAGGCAGAGGGAGTGACTGGTCTTGCGCTAGCCACTGG
  CTGACTCCGTAGGCCTGACACAGTCTACACCACAACACCTGAGCGTATTTTCCTTTGCTA
  CCGATGGGGGTCTGANAGCGCACGAGGGNAGGAATTGCTTANTCCACATCAAGAGCCTCT
  GCACTGGGTACTACATGGGATCGTACACGGAGGGGGGAAGAAAATGATTCCTTCTGGCTA
  TTGCAAATTTCGCCCGATTGGCCGTGGTCTTTACATAAAGCAATTTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002945
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002945.2, NP_002936.1
• RefSeq Size: 2824 bp
• RefSeq ORF: 1851 bp
• Locus ID: 6117
• UniProt ID: P27694
• Cytogenetics: 17p13.3
• Domains: tRNA_anti, Rep-A_N
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: DNA replication, Homologous recombination, Mismatch repair, Nucleotide excision repair
• Gene Summary: This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways. [provided by RefSeq, Sep 2017]