Stromal interaction molecule 1 (STIM1) (NM_003156) Human Untagged Clone

CAT#: SC118136

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STIM1 (untagged)-Human stromal interaction molecule 1 (STIM1)



  "NM_003156" in other vectors (6)

Reconstitution Protocol

USD 942.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol STIM1
Synonyms D11S4896E; GOK; IMD10; STRMK; TAM; TAM1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_003156 edited
GAATTCGGCACGAGGCTTGCCTGGAGACCGTCGGCTGCACTCCCGGGCTCCTGGCTTTGCCTCTGGGATC
CCGAGGTGTCCACATCAGACGCATGTTGACTGAGACCTAGAGTCATGGATGTATGCGTCCGTCTTGCCCT
GTGGCTCCTCTGGGGACTCCTCCTGCACCAGGGCCAGAGCCTCAGCCATAGTCACAGTGAGAAGGCGACA
GGAACCAGCTCGGGGGCCAACTCTGAGGAGTCCACTGCAGCAGAGTTTTGCCGAATTGACAAGCCCCTGT
GTCACAGTGAGGATGAGAAACTCAGCTTCGAGGCAGTCCGTAACATCCACAAACTGATGGACGATGATGC
CAATGGTGATGTGGATGTGGAAGAAAGTGATGAGTTCCTGAGGGAAGACCTCAATTACCATGACCCAACA
GTGAAACACAGCACCTTCCATGGTGAGGATAAGCTCATCAGCGTGGAGGACCTGTGGAAGGCATGGAAGT
CATCAGAAGTATACAATTGGACCGTGGATGAGGTGGTACAGTGGCTGATCACATATGTGGAGCTGCCTCA
GTATGAGGAGACCTTCCGGAAGCTGCAGCTCAGTGGCCATGCCATGCCAAGGCTGGCTGTCACCAACACC
ACCATGACAGGGACTGTGCTGAAGATGACAGACCGGAGTCATCGGCAGAAGCTGCAGCTGAAGGCTCTGG
ATACAGTGCTCTTTGGGCCTCCTCTCTTGACTCGCCATAATCACCTCAAGGACTTCATGCTGGTGGTGTC
TATCGTTATTGGTGTGGGCGGCTGCTGGTTTGCCTATATCCAGAACCGTTACTCCAAGGAGCACATGAAG
AAGATGATGAAGGACTTGGAGGGGTTACACCGAGCTGAGCAGAGTCTGCATGACCTTCAGGAAAGGCTGC
ACAAGGCCCAGGAGGAGCACCGCACAGTGGAGGTGGAGAAGGTCCATCTGGAAAAGAAGCTGCGCGATGA
GATCAACCTTGCTAAGCAGGAAGCCCAGCGGCTGAAGGAGCTGCGGGAGGGTACTGAGAATGAGCGGAGC
CGCCAAAAATATGCTGAGGAGGAGTTGGAGCAGGTTCGGGAGGCCTTGAGGAAAGCAGAGAAGGAGCTAG
AATCTCACAGCTCATGGTATGCTCCAGAGGCCCTTCAGAAGTGGCTGCAGCTGACACATGAGGTGGAGGT
GCAATATTACAACATCAAGAAGCAAAATGCTGAGAAGCAGCTGCTGGTGGCCAAGGAGGGGGCTGAGAAG
ATAAAAAAGAAGAGAAACACACTCTTTGGCACCTTCCACGTGGCCCACAGCTCTTCCCTGGATGATGTAG
ATCATAAAATTCTAACAGCTAAGCAAGCACTGAGCGAGGTGACAGCAGCATTGCGGGAGCGCCTGCACCG
CTGGCAACAGATCGAGATCCTCTGTGGCTTCCAGATTGTCAACAACCCTGGCATCCACTCACTGGTGGCT
GCCCTCAACATAGACCCCAGCTGGATGGGCAGTACACGCCCCAACCCTGCTCACTTCATCATGACTGACG
ACGTGGATGACATGGATGAGGAGATTGTGTCTCCCTTGTCCATGCAGTCCCCTAGCCTGCAGAGCAGTGT
TCGGCAGCGCCTGACGGAGCCACAGCATGGCCTGGGATCTCAGAGGGATTTGACCCATTCCGATTCGGAG
TCCTCCCTCCACATGAGTGACCGCCAGCGTGTGGCCCCCAAACCTCCTCAGATGAGCCGTGCTGCAGACG
AGGCTCTCAATGCCATGACTTCCAATGGCAGCCACCGGCTGATCGAGGGGGTCCACCCAGGGTCTCTGGT
GGAGAAACTGCCTGACAGCCCTGCCCTGGCCAAGAAGGCATTACTGGCGCTGAACCATGGGCTGGACAAG
GCCCACAGCCTGATGGAGCTGAGCCCCTCAGCCCCACCTGGTGGCTCTCCACATTTGGATTCTTCCCGTT
CTCACAGCCCCAGCTCCCCAGACCCAGACACACCATCTCCAGTTGGGGACAGCCGAGCCCTGCAAGCCAG
CCGAAACACACGCATTCCCCACCTGGCTGGCAAGAAGGCTGTGGCTGAGGAGGATAATGGCTCTATTGGC
GAGGAAACAGACTCCAGCCCAGGCCGGAAGAAGTTTCCCCTCAAAATCTTTAAGAAGCCTCTTAAGAAGT
AGGCAGGATGGGGTGGCAGTAAAGGGACAGCTTGTCCTTCCCTGGGTGTTCTGTCTCTCCTTCCCTCCCT
TCCTTCAAGATAACTGGCCCCAAGAGTGGGGCATGGGAAGGGCTGGTCCAGGGGTCTGGGCACTGTACAT
ACCTGCCCCCTCATCCTTGGGTCCTTCATTATTATTTATTAACTGACCACCATGGCCTGCCTGCCCTGCC
TCCGTCCCAACCATGGGCTGCTGCTGTCACTCCCTCTCCACTTCAGTGCATGTCTTAGTTGCTGTTCCCT
CAGCTCCCAGCTCCACCTCTGGGGTTCAGCTTCTGTCTCTGCTGTCCCAGTTTTGAGGTTTGGTTTCTTG
TTTCTGTCTCTTGCTTTCGGGCTCCTCCCTCCCACCACTCCCCAACTTCCCCTAGCAGTTGCAGGGAAGA
TAGGACGAGTAGCTTCTGACATGTGTGCCTCAGATCTGTTCCACCCCACTCACAGTGGTTCTGTTTGCTC
CAGACTGGGGCTAGGGCCTAATCTTTGAAGTTTGTTCTTTGGTATTGATGTGGGTCAGAAGGAGCCTCAT
CCTAATCTCACTCAGGCCTCCAGGGATCCATGGGGGAGTGAAACCAATTCTCAGAGAACAACCCACCAGA
GACTTTTAAAGAGAGGCCAGGCTTGGGAATGGGTTGGGAGAGGCATCTGTTCATTGGAGCATGAGTGGAT
GCCAGAACTGTAGGTTATAAGGCAGTCACTTTTTCTCTCTACTCCCACCCACACCTGCCTCCCTCTTACC
CCTGCTCCCCCACACTGCAGGAGGATTTGTCTCTAAGAGGTGCTGCCCCAAAGCTCCCCAAGCATCAATA
CTCCTAGGGCTCAGGACAAGTGGCTCCCCTGGCCAGGAGAGCCACAGCCATGATACAGGGCTCTTATGGA
GCCCTGGAGTTGTTGGGCAAGGATGCTGTCATTTTTTGAACCAAAAGACAAACAGGTTAAAAGGAAAAAA
AGTAATCTGAATTTCCCAAGTGCCTACGCTGCATATTCCCCTTGTTAGATCCCATTTTCATGTTACTTTG
TAGCCTTGGCCAGAGGCTCAAAAAGGACACAACCAGTTTGGGGAAGGGGTGGCTAAGGAAGATGGTATAG
GTGAAGGCGGCTGTGTGACCACTTTCCCCCACCCTTCCCACCCTCTAGACAACTCTCTCCCTTACCTGTT
TTTGCTATGGCTGTAAAGGTATTTTTCCTCTGCCCCACTCCCTGCCATACCTTTATCCTGGGATCCTATT
TTGGGCCTGGGGTGGGTATACCTGGGGCTGGTCTTAGGAGGGTGCTAGGCTGCAGACTGCCTTGTACTCC
CTGGACACCCTCAAATGGGGTTTTCTGTGTTATTTCATAAAATTCTTTGAAGTCCAATAAAGCATGTAGG
AGATTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
>OriGene 5' read for NM_003156 unedited
NGGGGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTTGC
CTGGAGACCGTCGGCTGCACTCCCGGGCTCCTGGCTTTGCCTCTGGGATCCCGAGGTGTC
CACATCAGACGCATGTTGACTGAGACCTAGAGTCATGGATGTATGCGTCCGTCTTGCCCT
GTGGCTCCTCTGGGGACTCCTCCTGCACCAGGGCCAGAGCCTCAGCCATAGTCACAGTGA
GAAGGCGACAGGAACCAGCTCGGGGGCCAACTCTGAGGAGTCCACTGCAGCAGAGTTTTG
CCGAATTGACAAGCCCCTGTGTCACAGTGAGGATGAGAAACTCAGCTTCGAGGCAGTCCG
TAACATCCACAAACTGATGGACGATGATGCCAATGGTGATGTGGATGTGGAAGAAAGTGA
TGAGTTCCTGAGGGAAGACCTCAATTACCATGACCCAACAGTGAAACACAGCACCTTCCA
TGGTGAGGATAAGCTCATCAGCGTGGAGGACCTGTGGAAGGCATGGAAGTCATCAGAAGT
ATACAATTGGACCGTGGATGAGGTGGTACAGTGGCTGATCACATATGTGGAGCTGCCTCA
GTATGAGGAGACCTTCCGGAAGCTGCAGCTCAGTGGCCATGCCATGCCAAGGCTGGCTGT
CACCAACACCACCATGACAGGGACTGTGCTGAAGATGACAGACCGGAGTCATCGGCAGAA
GCTGCAGCTGANAGCTCTGGATACAGTGCTCTNTGGGCCTNCTCTCTTGACTCGCCATAA
TCACCTCAAGGACTTCATGCTGGNNTGGTGTCTATCGTTATTGGGTGTGGGCCGCTGCTT
GGTTTGCCTATATCCAGAACCGTTACTNCCAGGAGCACATGAANAAAATGATGAAGGACT
TTGGAGGGNTACACCGAGCTA
>OriGene 3' read for NM_003156 unedited
ACTATGGAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTAAAATCTCCTACATGCTTTATTGGACTTCAAAAAATTTTATGAAATAACACAGAAAA
ACCCCATTTGAGGGTGTCCAGGGAGTACAAGGCAGTCTGCAGCCTAGCACCCTCCTAAGA
CCAGCCCCAGGTATACCCACCCCAGGCCCAAAATAGGATCCCAGGATAAAGGTATGGCAG
GGAGTGGGGCAGAGGAAAAATACCTTTACAGCCATAGCAAAAACAGGTAAGGGAGAGAGT
TGTCTAAAGGGTGGGAAGGGTGGGGGAAAGTGGTCACACAGCCGCCTTCACCTATACCAT
CTTCCTTAGCCACCCCTTCCCCAAACTGGTTGTGTCCTTTTTGAGCCTCTGGCCAAGGCT
ACAAAGTAACATGAAAATGGGATCTAACAAGGGGAATATGCAGCGTAGGCACTTGGGAAA
TTCAGATTACTTTTTTTCCTTTTAACCTGTTTGTCTTTTGGTTCAAAAAATGACAGCATC
CTTGCCCAACAACTCCAGGGCTCCATAAGAGCCCTGTATCATGGCTGTGGCTCTCCTGGC
CAGGGGAGCCACTTGTCCTGAGCCCTAGGAGTATTGATGCTTGGGGAGCTTTGGGGCAGC
ACCTCTTAGAGAAAATCCTCCTGCAGTGTGGGGGAGCAGGGGTAANAAGGAGGCAGGTGT
GGGTGGGAGTANAAAGAAAAAGTGACTGCCTTATAACCTACAGTTCTGGCATCCACTCAT
GCTCCAATGAACAAGATGCCTCTCCCAACCCATTTCCAAGCCTGGCCTTTCTTTAAAGTC
TCTGGTGGGGTGTTCTCTGAAAAATGGTTTCACCTCCCCATGGATCCCTGGAAGGCTGAA
TGAAAATAAGAGGAGGCTCCTTCTGACCCCATCAATCCAAAGACAAACTTCAAAAATAGG
CCCTAGCC
Restriction Sites NotI-NotI     
ACCN NM_003156
Insert Size 4000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003156.2, NP_003147.2
RefSeq Size 4039 bp
RefSeq ORF 2058 bp
Locus ID 6786
UniProt ID Q13586
Cytogenetics 11p15.4
Protein Families Transmembrane
Gene Summary This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
Transcript Variant: This variant (2, also known as STIM1S) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.

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